Agenda Item 65/36 - Annexure - 32

BBIT307L Plant Biotechnology L T P C

3 0 0 3
Pre-requisite BBIT207L, BBIT207P Syllabus version
1.0
Course Objectives
1. Explain the plant tissue culture methods.
2. Build knowledge of biotechnological tools which help in modifying plants suited to
agriculture.
3. Elaborate the production of biopharmaceuticals in plants.

Course Outcomes
1. Develop key concepts on genome organization in plants.
2. Apply plant tissue culture to develop genetically engineered plants.
3. Analyze the various components involved in developing transgenic plants.
4. Elaborate the production of new biomolecules in plant using transgenic technology.
5. Compare and apply molecular marker technology in plant breeding.

Module:1 Plant Genome Organization and Expression 5 hours

Introduction to gene structure and gene expression, Regulation of gene expression; Protein
targeting; Genome size and organization; Chloroplast and Mitochondrial genome.
Module:2 Plant Tissue Culture 6 hours
Plasticity and totipotency; Culture environment, Culture media, Plant growth regulators and
their applications; Culture types, Regeneration (somatic embryogenesis and organogenesis)
and hardening; Applications of plant tissue culture.
Module:3 Techniques for Plant Transformation 6 hours
Plant transformation - Direct methods (particle bombardment, PEG mediated transformation,
Electroporation, Silicon carbide fibre), Indirect methods (Agrobacterium, hairy root and in
planta transformation).
Module:4 Vectors for Plant Transformation 6 hours
Binary vectors, Gateway cloning and RNAi vectors, Promoters, Terminators, Markers
involved in designing an expression cassette, Reporter genes, Gene silencing, Clean gene
technology, Plastid transformation.
Module:5 Transgenes for Herbicide, Pest and Disease Tolerance 6 hours
Herbicide tolerance (Glyphosate, Phosphinothricin, Imidazolinone), Pest resistance (Cry
gene, copy nature strategy), Disease resistance (BASF potato).
Module:6 Transgenes for Stress Tolerance and High Crop Yield 6 hours
Stress tolerance (Biotic and Abiotic stress), Crop yield and quality (flavr savr tomato, golden
rice).
Module:7 Molecular Farming & Molecular Markers 5 hours
Carbohydrates and lipids (Starch, Polyfructans and Bioplastics), Proteins (Hirudin and
Insulin), Custom made antibodies, Edible vaccines, Production of secondary metabolites.
RAPD, RFLP, AFLP, SSR, ISSR, QTL mapping, Marker assisted selection and map based
cloning.
Module:8 Contemporary Issues 5 hours

Total Lecture hours: 45 hours

Text Book(s)
1. Plant Biotechnology: The Genetic Manipulation of Plants by Adrian Slater, Scott N W,
Fowler M, 2nd Edition, 2015, Oxford University Press, New Delhi, India.
Reference Book(s)

Proceedings of the 65th Academic Council (17.03.2022) 677

 Agenda Item 65/36 - Annexure - 32

1. Plant Biotechnology: Principles and Applications by Malik Zainul Abdin, Usha Kiran, M.
Kamaluddin, Athar Ali, 1st Edition, 2017, Springer Nature, Singapore.
2. Plant Biotechnology: Recent Advancements and Developments by Suresh Kumar
Gahlawat, Raj Kumar Salar, Priyanka Siwach, Joginder Singh Duhan, Suresh Kumar
and Pawan Kaur, 1st Edition, 2017, Springer Nature, Singapore.
Mode of Evaluation: CAT, Assignment, Quiz and FAT

Recommended by Board of Studies 18-02-2022

Approved by Academic Council No. 65 Date 17-03-2022

Proceedings of the 65th Academic Council (17.03.2022) 678