Standard [PNP] (μM) Trial 1(A) Trial 2 (A)

Standard 1 10 0.039 0.039

Standard 2 20 0.075 0.075
Standard 3 30 0.127 0.127
Standard 4 50 0.201 0.201
Standard 5 75 0.314 0.314
Unknown 0.332 0.332

Standard [PNP] (μM) Average Ab. Standard Dev.

Standard 1 10 0.039 0
Standard 2 20 0.075 0
Standard 3 30 0.127 0
Standard 4 50 0.201 3.399349889E-17
Standard 5 75 0.314 0
Unknown 0.3316666666667 0.0005773502692
Trial 3 (A)
0.039
0.075
0.127
0.201
0.314
0.331

x y
0 0.332
80.285 0.332
80.285 0

PNP Concentration Vs Absorbance

0.35
f(x) = 0.00422686567164179 x − 0.00519402985074624
R² = 0.998272364908395 5; 0.314
0.3

0.25

0.2 4; 0.201 Standard Sample

Absorbance

Linear (Standard Sample)

0.15 Unknown Sample
3; 0.127
0.1
2; 0.075
0.05
1; 0.039

0
0 10 20 30 40 50 60 70 80 90
PNP Concentration
Standard Sample
Linear (Standard Sample)
Unknown Sample
pH Trial 1(A) Trial 2 (A) Trial 3 (A)
4 0.07 0.06 0.06
6 0.038 0.038 0.038
7 0.207 0.207 0.207
7.64 0.351 0.351 0.351
8 0.367 0.368 0.368
8.43 0.458 0.458 0.458
9 0.388 0.388 0.388
10 0.443 0.443 0.443
Mid Point = 0.21 Pka =

pH Vs Absorbance
0.500
0.450
0.400
0.350
Absorbance

0.300
0.250
0.200
0.150
0.100
0.050
0.000
3 4 5 6 7 8 9 10
pH Values
 Standard
Average Ab.
Deviation
0.063 0.006
0.038 0.000
0.207 0.000
0.351 0.000
0.368 0.001
0.458 0.000
0.388 0.000
0.443 0.000
6.706

sorbance

7 8 9 10 11
H Values