SUPPLEMENTAL FIGURES AND LEGENDS

Supplemental movie legend

Supplemental Movie S1. A representative approach-contact-retraction cycle of a PMN moving inside a
micropipette. A WM9 melanoma cell was held steadily by a micropipette on the right side.

Supplemental figures
A 1.0
Adhesion Probability Pa (%) PMN vs. 110 U/ml TNF--stimulated WM9
PMN vs. Quiescent WM9
0.8

0.6

0.4

0.2
Nonspecific

0.0
0 5 10 15 20 25
Contact Duration (s)

B 1.0
PMN vs. 300 U/ml TNF--stimulated HPMEC
Adhesion Probability Pa (%)

PMN vs. Quiescent HPMEC

0.8

0.6

0.4

0.2

0.0 Nonspecific
0 5 10 15 20 25
Contact Duration (s)

Supplemental Fig. S1. Binding curves of PMN-WM9 (A) and PMN-HPMEC (B) adhesions when WM9
or HPMEC cells were quiescent (squares) or pre-incubated by TNF-(cycles). Data were binned per
contact event upon similar contact durations and presented as the means ± SEs of contact durations. Also
plotted were the predictions fitted using Eq. 1 (solid lines) while the dashed lines represented nonspecific
binding, obtained by fitting Eq. 1 to nonspecific data (not shown for the sake of clarity).

1
 A 1.0 B 1.0
PMN vs. Quiescent WM9 PMN vs. 110 U/ml TNF--stimulated WM9
Adhesion Probability Pt (%)

Adhesion Probability Pt (%)

0.8 0.8

0.6 0.6

0.4 0.4

0.2 0.2
Nonspecific Nonspecific

0.0 0.0
0 5 10 15 20 25 0 5 10 15 20 25
Contact Duration (s) Contact Duration (s)

C 1.0 D 1.0
PMN vs. Quiescent HPMEC PMN vs. 300 U/ml TNF--stimulated HPMEC
Adhesion Probability Pt (%)

Adhesion Probability Pt (%)

0.8 0.8

0.6 0.6

0.4 0.4

0.2 0.2

0.0 Nonspecific 0.0 Nonspecific

0 5 10 15 20 25 0 5 10 15 20 25
Contact Duration (s) Contact Duration (s)

E 1.0
PMN vs. Quiescent MDA-MB-231
Adhesion Probability Pt (%)

0.8

0.6

0.4

0.2

Nonspecific
0.0
0 5 10 15 20 25
Contact Duration (s)

Supplemental Fig. S2. The 95% confidence intervals (dashed lines) of the best fitted curves (solid
lines) from experimental data (points) for PMN vs. quiescent (A) and TNF--stimulated WM9 (B), PMN
vs. quiescent and TNF--stimulated HPMEC (D), and PMN vs. quiescent MDA-MB-231 (E). The
nonspecific bindings were presented by grey lines.