Final Full Guideline Book ICAVESS 2021

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GUIDELINE BOOK
THE 1ST INTERNATIONAL CONFERENCE OF ADVANCED VETERINARY
SCIENCE AND TECHNOLOGIES FOR SUSTAINABLE DEVELOPMENT
(1ST ICAVESS)
Presented by :
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia

Office : Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta (55281)
Email : icavess@ugm.ac.id
Contact : +6281296860904
Asian Association of Veterinary Schools (AAVS)

Office : secretary@aavs.jpn.org
OIE Regional Representation for Asia and the Pacific

Office : asia.pacific@oie.int
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SCIENTIFIC COMMITTEE
Prof. Dr. Siti Isrina Oktavia Salasia, DVM. Faculty of Veterinary Medicine, Universitas
Gadjah Mada
Dr. med.vet. Liliana Machado Ribeiro da Institute of Parasitology, Justus Liebig University
Silva Giessen, Giessen, Germany
Prof. Teguh Budipitojo, DVM., M.P., Ph.D Department of Anatomy, Faculty of Veterinary
Medicine, Universitas Gadjah Mada
Tamara Muñoz-Caro, DVM, M.Sc,. Ph.D Universidad Santo Tomás, Chile
Noer Kasanah, S.Si., M.Si., Apt., Ph.D Department of Fisheries Faculty of Agriculture,
Universitas Gadjah Mada
Prof. Dr.med.vet.. Dr. habil. Carlos Institute of Parasitology, Justus Liebig University
Hermosilla, DVM., DipEVPC Giessen
Prof. Tety Hartatik, S.Pt., Ph.D. Department of Animal Breeding and
Reproduction, Faculty of Animal Science,
Prof. Michael P Reichel VS, Dipl VPH, Dipl Adjunct Professor
VTM, MVSc, MBA, Dr med vet, PhD, Department of Population Medicine & Diagnostic
FACVSc, FRCVS Science, College of Veterinary Medicine, Cornell
University, Ithaca, New York, USA
Dr. Widagdo Sri Nugroho, DVM., M.P. Department of Epidemiology and Public Health,
Faculty of Veterinary Medicine, Gadjah Mada
University
PD Dr. rer. nat. habil. Sonja Kleinertz DAAD Long Term Lecturer, Germany
IPB University, Indonesia
Dr. Tri Rini Nuringtyas, S.Si, M.Sc Departement of Biochemistry, Faculty of Biology,
Universitas Gadjah Mada, Indonesia
Assistant Prof. Dr. Chenpop Sawamangke, Veterinary Stem Cell and Bioengineering Center
DVM., M.Sc, Ph.D (VSCBC), Faculty of Veterinary Science,
Chulalongkorn University
Prof. Dr. Wayan Tunas Artama, DVM. Head of the One Health Collaborating Centre,
Indonesia
Prof. Masatoshi Hori, DVM., Ph.D Department of Veterinary Medical Sciences,
Graduate School of Agriculture and Life Sciences,
The University of Tokyo
April Hari Wardhana, SKH, M.Si, PhD Indonesian Research Center for Veterinary
Science, Bogor, Indonesia
Dr. agr. Ir. Sigit Prastowo, S.Pt., M.Si., Department of Animal Science, Universitas
IPM. Sebelas Maret, Surakarta Indonesia
Dr. Gholib, S.Pt, M.Si Faculty of Veterinary Medicine Syiah Kuala
University
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ORGANIZING COMMITTEE
FACULTY OF VETERINARY MEDICINE

Division Name
Chairman Dr. Widagdo Sri Nugroho, DVM., M. P
Secretary I Dr. Vista Budiariati, DVM., M.Si
Secretary II Siti Hanifah, S.E.
Finance Wienarni, A.Md
Yekti Basuki, A.Md
Publication Erif Maha Nugraha Setyawan, DVM., M.Sc., Ph.D
Dr. Agustina Dwi Wijayanti, DVM., M. P
Prof. Dr. Aris Haryanto, DVM, M.Si
Dyah Ayu Widiasih, DVM., Ph.D
Endah Choiriyah, S.IP., M.Si
Public Relation and Promotion Marla Anggita, DVM., M.Sc
Okti Herawati, DVM., M.Sc
Event Dinar Arifianto, DVM., M.Sc
Arvendi Rachma Jadi, DVM., M.Sc
Topas Wicaksono Priyo Jr., DVM., M.Sc
Dwi Sunu Datrianto, DVM., M.Sc
Makruf Arif, DVM., M.Sc
Yonathan Alvin Maruli Asi Sihotang, DVM.
Amanda Yonica Poetri Faradifa, S.K.H.
Logistic and Equipment Bangun Murdianjati S.T
Handi Kurniawan, S.Kom
ASIAN ASSOCIATION OF VETERINARY SCHOOLS (AAVS)

Prof. Masatoshi Hori, DVM., Ph.D
Dr. Takeshi Haga, DVM., Ph.D
Dr. Maho Urabe (OIE Staff Supporting AAVS)
OIE REGIONAL REPRESENTATION FOR ASIA AND THE PACIFIC

Dr. Maho Urabe
Dr. Asako Endo Ozawa
Dr. Marina Fujii (Intern)
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TABLE OF CONTENTS
COVER PAGE ........................................................................................................... 2
SCIENTIFIC COMMITTEE .......................................................................................... 3
ORGANIZING COMMITTEE ...................................................................................... 4
WELCOME ADDRESS RECTOR UGM ........................................................................ 6
WELCOME ADDRESS DEAN OF FACULTY OF VETERINARY MEDICINE .................... 8
WELCOME ADDRESS PRESIDENT OF AAVS ............................................................. 10
SCHEDULE AT GLANCE ............................................................................................ 12
THEMATIC SESSION CLASS ...................................................................................... 18
ABSTRACTS (Zoonosis, Emerging Diseases, and Transboundary Diseases) ........... 43
ABSTRACTS (Biodiversity and Wildlife Conservation) ............................................ 65
ABSTRACTS (Livestock and Food Biosafety) ........................................................... 92
ABSTRACTS (Bioengineering and Biotechnology) ................................................... 122
ABSTRACTS (Sustainable and Renewable Energy from Animal Manure) ............... 144
ABSTRACTS (Advanced Veterinary Pharmacology and Sustainable Agriculture) ... 152
ABSTRACTS (Veterinary Medicine and Science) ..................................................... 164
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WELCOME ADDRESS
RECTOR OF UNIVERSITAS GADJAH MADA
Dear distinguished speakers, participants, ladies, and gentlemen,

On behalf of Universitas Gadjah Mada, I warmly welcome all of you today at the
1st International Conference of Advanced Veterinary Science and Technologies for
Sustainable Development (ICAVESS) 2021, organized by the Faculty of Veterinary
Medicine, Universitas Gadjah Mada.
I would like to take this opportunity to congratulate the Faculty of Veterinary
Medicine UGM in collaboration with the Asian Association of Veterinary School
Association (AAVS) for organizing this international conference online.
I am proud to introduce Universitas Gadjah Mada briefly to all of you. Universitas Gadjah
Mada is the oldest and the largest university in Indonesia and the best University in
Indonesia 2020 (According to The Ministry of Education and Culture of the Republic of
Indonesia). Since its early establishment, UGM is the place to learn for the young
generation from various regions in Indonesia with their distinctive culture and local
languages.
UGM is implementing an educopolis area, a step taken by universities in creating
a conducive environment for the continuity of the learning process. These efforts are
pursued in the context of developing multidisciplinary collaborations and responding to
ecological issues to achieve the university's vision. UGM has engaged in a dedicated
ecological development program by restricting vehicles from entering the campus,
constructing new "green" dormitories on campus, and reducing pollution.
The COVID-19 pandemic has caused changes in human life around the world.
UGM is also working hard to control the outbreak by producing various innovative
diagnostic tools such as the GeNose C19 and various research involving multiple
disciplines. This shows that scientific collaboration is a necessity for present life and the
future.
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We may take the best of advanced sciences and technologies nowadays, but
avoiding environmental degradation is mandatory to guarantee natural resources
inherited by our succeeding generation. The 1st ICAVESS concerns on the global change
of climate and anthropogenic factors which are unavoidably lead to significant changes
in diseases distribution worldwide such as COVID-19. In this context, the bridge of
multidisciplinary studies is needed not only as a responsive reaction but also to develop
sustainable strategies that may spread across continent and administrative boundaries.
The concept of sustainable development is mainly inspiring ICAVESS as "the
development that meets the needs of the present without compromising future
generations' ability to meet their own needs". For the 1st ICAVESS 2021, the general
theme is "Synergizing Sciences for Sustainable Animal, Human and Environmental
Qualities in Global Change".
We are hopeful ICAVESS will significantly inspire other academicians or
researchers to achieve an even more significant impact in life sciences. UGM is proud to
be leading the way in facilitating the interdisciplinary research dissemination of cutting-
edge information. We are pleased to conduct this conference, even-though this
conference holds virtually. Still, we hope it could be strengthening our institutional
collaboration, especially in veterinary, life sciences, and the environment.
Finally, I wish you a pleasant and enjoyable conference.
Thank you very much for your attention.
Prof. Ir. Panut Mulyono, M.Eng., D.Eng.
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WELCOME ADDRESS
DEAN OF FACULTY OF VETERINARY MEDICINE UGM
On behalf of the Dean of Faculty of Vet Medicine UGM and the President of the
Indonesian Association of Veterinary Schools (IAVS), I would like to wish you a warm
welcome to the "First International Conference of Advanced Veterinary Science and
Technologies for Sustainable Development 2021" (1st ICAVESS 2021). This conference
collaboration with the Asian Association of Veterinary Schools (AAVS).
It is truly an honor and privilege for us to have the opportunity to speak and
welcome you all to this wonderful occasion. Even though the world is still facing crises
due to disruption caused by COVID-19, we are grateful for organizing our first ICAVESS
and having the opportunity to share it with you all through the virtual system.
The 1st ICAVESS 2021 offers the theme of "Synergizing Sciences for Sustainable
Animal, Human and Environmental Qualities in Global Change". In this event, we invited
eight prominent scientists in their respective fields from Japan, Germany, USA, Thailand,
Malaysia, Korea, Indonesia, and the OIE Sub-Regional Representative for South-East
Asia. The speakers will share the recent advances in veterinary science and technology
and concern the global climate change and significant disease distribution worldwide.
This conference also certainly marks a special occasion for veterinary school
deans – an opportunity to discuss and share information, possible collaboration in
educational improvements in terms of the standardization system, globalization, and
challenges to Veterinary Education in Asia. I am sure that networking and collaboration
will be established among us, strengthening the future excellent veterinary education,
research, and technology for Indonesia and the world.
I would like to express my sincere appreciation to the committees who have
made the conference runs even in a virtual way. This event is the first ICAVESS
conference that is conducted on a digital platform in line with the social distancing
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norms due to the COVID-19 pandemic.
We sincerely hope that the symposium and all related activities will also produce
a positive impact and benefit for veterinary education, research, and possible
synergizing sciences for the sustainable animal, human and environmental qualities in
the world.
Thank you for joining the conference.
I do hope you will also take the time to enjoy this conference.
Prof. Dr. Siti Isrina Oktavia Salasia, DVM
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WELCOME ADDRESS
PRESIDENT OF ASIAN ASSOCIATION OF VETERINARY SCHOOLS (AAVS)
I am Masatoshi Hori, Head of the Department of Veterinary Medical Science, the

University of Tokyo and, also I am a President of Asian Association of Veterinary Schools
(AAVS).
Congratulations on hosting the 1st International Conferences of Advanced
Veterinary Sciences and Technology for Sustainable Development (1st ICAVESS). I would
like to express my deepest gratitude to the Dean of the Faculty of Veterinary Medicine
Gadjah Mada University and also the Organizing Committee in this meeting. Due to
COVID-19 pandemic, the event was postponed last summer. This time, it will be held
online. But, the fact the ICAVESS will be hosted is due to the effort of committee
members involved. Thank you again.
By the way, I am myself had never been visited Indonesia and was looking for
visiting Indonesia at this time. Because, my wife, she grew up in Jakarta as a child for 6
or 7 years. She often told me about Indonesia’s greatness especially nature, food, and
kind humanity for me. So, this time I will be participating by an online system. I will
definitely like to visit Indonesia and Gadjah Mada University. Dr. Koichi Sato, the Dean
of faculty of veterinary medicine of Yamaguchi University, is an important collaborator
for Gadjah Mada University. At the same time, he is my longtime friend. So I’m thinking
of having him escort me.
In recent 20 years, the role that veterinarians should play in the world is
increasing year by year. There is an urgent need for veterinary education, veterinary
research, and veterinary human resource development in Asia, such as infectious
disease problems, food safety, food security, drug-resistant bacteria, and so on.
At the United Nations Summit in September 2015, the "2030 Agenda for
Sustainable Development" was unanimously adopted by the member states. So it is the
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Sustainable Development Goals (SDGs), the international goal for a sustainable and
better planet by 2030. SDGs are important for global conservation including humankind.
I have high hopes that this ICAVESS meeting and AAVS will become one of the core and
contribute to SDGs mediated by veterinarians in the future.
Finally, in the last 1 year and 4 months, many people around the world have died
of SARS-CoV-2 infection. I would like to conclude my greeting with my condolences. And
I expect that we will build a new world order with Corona and move forward together
for the future.
Prof. Masatoshi Hori, DVM., Ph.D.
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SCHEDULE AT GLANCE
The 1st International Conference of Advanced Veterinary Science and
Technologies for Sustainable Development (1st ICAVESS 2021)
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta
DAY 1: Sunday, March 28th 2021

Platform: Zoom Meeting
Zoom meeting link: http://ugm.id/ICAVESSPlenary1
(Meeting ID: 860 8180 5689; Passcode: ICAVESS01)
Youtube Livestreaming link: http://ugm.id/ICAVESS2021D1
TIME (GMT+7) PROGRAM PIC

Registration & Opening
Organizing committee
(OC) ICAVESS
07.30-07.45 Registration (Host accepts participants
to enter the
room)
MC :
Welcome Greeting
Kharisma Dewi, DVM.
National Indonesia Antheme (Indonesia MC : Kharisma Dewi,
Raya) DVM. and Host
- Dean of FoVM UGM
07.45-08.20 Welcome and opening speech - President of AAVS
- Rector of UGM
Traditional Dance MC : Kharisma Dewi,
Klana Topeng DVM. and Host
MC :
Opening and schedule of the day
Kharisma Dewi, DVM.
Plenary Session Day 1
Prof. Michael P. Reichel
(Population Medicine and
Diagnostic, Australia) Moderator:
08.20-09.00 “Global impact of infectious diseases Prof. Teguh Budipitojo,
(40 Minutes) challenges and opportunities, DVM, M.P., Ph.D
African Swine Fever and
Coronavirus on point”
(30’ presentation 10’ discussion)
09.00-09.15 Session break all host and co host
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Prof. Carlos Hermosilla
(Justus Liebig Universitaet Giessen,
Germany)
09.15-09.55
“Host-pathogen interaction and Moderator:
(40 Minutes)
arise of neglected tropical diseases Prof. Teguh Budipitojo,
due to global change” DVM, M.P., Ph.D
(30’ presentation; 10’ discussion)
Assistant Prof. Dr. Chenpop
Sawangmake (Veterinary Stem Cell and
Bioengineering Innovation Center
(VSCBIC); Faculty of Veterinary
09.55-10.35 Science, Chulalongkorn University,
(40 Minutes) Thailand)
“Stem cells in vet: from science to
future hold to make a better world”
(30’ presentation; 10’ discussion)
10.35-10.40 Photo Session & Closing of Plenary MC :
Session 1 Kharisma Dewi, DVM.
AAVS Vet Education Session
Asian Association of Veterinary Schools
Moderator:
(AAVS) Vet Education session
Dr. Omar, AAVS Vice
1) Introduction by Session Chair
President
(Dr. Omar, AAVS Vice
President (5 Minutes))
Invited AFKHI (Indonesia
2) Professor Dato' Dr Mohd
Assoc of Vet
Hair bin Bejo (Faculty of
School) Dean to join
10.40-12.00 Veterinary Medicine,
Globalisation and
(80 Minutes) Universiti Putra Malaysia
Challenges to
(30 minutes)
Veterinary Education in
3) Pan-Dong Ryu, DVM, PhD
Asia
College of Veterinary
Veterinary education and
Medicine Seoul National
accreditation of
University
veterinary schools in Asia
(30 minutes)
(Live)
Q&A session (15 minutes)
Closing of AAVS Vet Education Session MC :
Kharisma Dewi, DVM.
12.00-13.00 BREAK all host and co host
Parallel Session
13.00-16.00 Thematic session Moderator, Cohost,
(Parallel zoom rooms) Organizing committee
16.00-16.10 Closing 1st ICAVESS 2021 DAY 1 (OC) ICAVESS
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DAY 2: Monday, March 29th 2021
Platform: Zoom Meeting
Zoom meeting link: http://ugm.id/ICAVESSPlenary2
(Meeting ID: 820 5389 9239; Passcode: ICAVESS02)
Youtube Livestreaming link: http://ugm.id/ICAVESS2021D2
TIME
PROGRAM PIC
(GMT+7)
Registration & Opening
07.30-08.00 Registration Organizing committee (OC) ICAVESS
MC :
08.00-08.10 Opening, schedule of the day
Kharisma Dewi, DVM.
Plenary Session Day 2
Prof. Masatoshi Hori
(Graduate School of Agriculture
and Life, Uni Tokyo, Japan) Moderator:
08.10-08.50 “Multifunctional antioxidant Dyah Ayu Widiasih, DVM,
(40 Minutes) phytochemical, γ-oryzanol Ph.D
extracted from rice bran”
08.50-09.00 Session break all host and co host
PD Dr. rer. nat. habil. Sonja
Kleinertz
“Ecological Relevance of Marine
09.00-09.40 Fish Parasites: Modern
(40 Minutes) Perspectives in Fisheries and
Ecosystem Approach Moderator:
(30’ presentation 10’ discussion) Dyah Ayu Widiasih, DVM,
Ph.D
09.40-10.20 Prof. Wayan Tunas Artama
(40 Minutes) (Head of One Health Collaborating
Centre, Indonesia)
“Current situation of zoonotic
diseases in Indonesia”
Closing of Plenary Session 1 MC : Kharisma Dewi, DVM.
AAVS-OIE-UGM Session
Dr. Motohiro Hirouchi Moderator
Opening remarks on behalf of AAVS & Dr. Motohiro Horiuchi
10.20-10.25
Brief introduction of the session Dean of Faculty of Veterinary
Medicine, Hokkaido University
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Dr. Ronella Abila, OIE
(Presentation “Experience in SEA to Invited AFKHI (Indonesia Assoc of
10.25-10.40 improve veterinary curricula using the Vet
OIE Guidelines”) School) Dean to join
Experience sharing by AAVS Members

(Part 1) “Collaboration between
Dr. Tongkorn Meeyam international
10.40-11.00
(Chiang Mai University, Thailand) organizations and veterinary
Dr. Le Quang Thong education
(Nong Lam University, Vietnam) establishments in Asia”
11.00-11.10 Q&A
WHO (Dr. Gyanendra Gongal)
FAO (Dr. Filip Claes)
OIE (Dr. Maho Urabe)
11.10-11.25
(Brief introduction on OIE/FAO/WHO
Reference Centers and other scientific
activities with VEEs)
Experience sharing by AAVS Members
(Part 2)
Dr. Naoaki Yokoyama
11.25-11.45
(Obihiro University, Japan)
Dr. Rungtip Chuanchuen
(Chulalongkorn University, Thailand)
11.45-11.55 Q&A
Dr. Hirofumi Kugita, OIE
Prof. Dr. Siti Isrina Oktavia Salasia,
11.55-12.00
DVM, Dean of FVM UGM
(Closing remarks)
Closing of AAVS-OIE-UGM Session MC :
Kharisma Dewi, DVM.
12.00-13.00 BREAK all host and co host
Thematic session Moderator, Cohost,
13.00-15.00
(Parallel zoom rooms) Organizing committee (OC) ICAVESS
16.00-16.10 1st ICAVESS 2021 closing ceremony
1. Closing Dance “Mangastuti”
2. Announcement of Best Presenter In MC : Kharisma Dewi, DVM. and
Parallel Session Class Host
3. Closing remark by AAVS representative
4. Closing remark by Dean of Faculty
Veterinary Medicine, Universitas
Gadjah Mada
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MEETING ROOMS
Day 1 1st ICAVESS 2021 Thematic Session Class
(Time 13.00-16.00 (GMT+7))
Room Meeting ID Pass Meeting link
code
1 867 7395 3869 ClassA http://ugm.id/icavessA
2 820 5389 9239 ClassB http://ugm.id/icavessB
3 953 6909 9650 ClassC http://ugm.id/icavessC
4 858 2869 4411 ClassD http://ugm.id/icavessD
5 889 5599 1151 ClassE http://ugm.id/icavessE
6 899 2522 5643 ClassF http://ugm.id/icavessF
Day 2 1st ICAVESS 2021 Thematic Session Class

(Time 13.00-15.00 (GMT+7))
Room Meeting ID Pass Meeting link

code
1 833 2761 6214 ClassG http://ugm.id/icavessG
2 864 8550 1587 ClassH http://ugm.id/icavessH
3 944 1187 6812 ClassI http://ugm.id/icavessI
4 826 1058 2535 ClassJ http://ugm.id/icavessJ
5 870 7124 5944 ClassK http://ugm.id/icavessK
6 849 1040 1432 ClassL http://ugm.id/icavessL
*Presenter should be join the parallel room 15 minutes

before the first presentation begins (12.45 Jakarta time/GMT+7)
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DAY 1: Sunday, 28th March 2021
Room 1 ClassA
Moderator 1 Putu Cri Devischa G, DVM, M.Sc
Moderator 2 Anggi Muhtar Pratama, DVM, M.Sc
Cohost1 Dwi Sunu, DVM, M.Sc
CoHost2 Amanda Y, SKH
Meeting Link http://ugm.id/icavessA
Meeting ID 867 7395 3869
Passcode ClassA
No. Time Paper Title Name Presenter

ID ID
1 13.00-13.08 38-008 Acute leptospirosis in working dogs in Jakarta Ambar A-001

after the flood using serological and molecular Retnowati
tests
2 13.09-13.16 38-014 Trematode and Nematode gastrointestinal Fitrine A-002

infestations in livestock from different Ekawasti
geographical regions in Indonesia
3 13.17-13.24 38-107 The Study of Immunity Duration Against Ambar A-003

Leptospirosis and Rabies Commercial Vaccination Retnowati
in Working Dogs
13.25-13.31 Q and A session
4 13.32-13.40 38-049 A Brief Discovery of the Plerocercoid Larvae of Gloria A-004

Spirometra sp. on Rice Frog from Local Traders in Animalesto
Bogor
5 13.41-13.49 38-072 Sero-surveillance and potential risk factors Saifur A-005

associated with avian influenza subtype H9N2 in Rehman
Backyard Poultry in the Desert Thal of District
Bhakkar Punjab Pakistan
6 13.50-13.58 38-073 African Swine Fever (ASF) : Threat of Extinction to Jonathan A-006
Nias Local Pig Farm Anugrah
Lase
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7 14.06-14.14 38-088 Detection and Genetic Variation of Leptospira sp. Tito A-007
in Cattle from the Slaughterhouse of Kediri and Suprayoga
Yogyakarta
8 14.15-14.23 38-089 Diversity of rodent species and its potency as the Herawati A-008
vector on transmitting rodent borne parasitic Yuwono
disease in households
9 14.24-14.32 38-096 The in Vitro Study of Potential Green Tea Leaves Bambang A-009
(Camellia sinensis) Water Extract as Anti Avian Sutrisno
Poxvirus on Egg Chicken
10 14.40-14.48 38-102 Phylogenetic and Genetic Diversity of Pathogenic Farida Dwi A-010
Leptospira from Leptospirosis Cases in Demak Handayani
Regency-Central Java and DKI Jakarta
11 14.49-14.57 38-094 Effect of D-galactose on Weight Gain in Animal Dwi G1-016

Model of Aging Datrianto
12 14.58-15.06 38-095 Dermatophytosis in Cats: Clinical Signs and Sitarina G1-017

Identification of Etiological Agent Widyarini
13 15.14-15.22 38-097 Re-Epithelization and Density Of Collagen Fibers Tri Wahyu G1-018
On Wound Healing Of Mice’s Skin (Mus Pangestining
musculus) That Treated With Combination Of sih
Chitosan Membrane and Eel (Monopterus albus)
Mucous
14 15.23-15.31 38-099 Detection of Ovine Herpesvirus-2 in Clinical Cases Harimurti G1-019

of Sheep-Associated Malignant Catarrhal Fever in Nuradji
Balinese Cattle and In Apparantly Healthy Sheep
in East Nusa Tenggara
15 15.32-15.40 38-104 Antibacterial Activity of Lactobacillus spp. Allysa G1-020

Bacteriocin Against Resistant Bacteria: A Review. Shavira
15.48-15.50 Closing
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DAY 1, Sunday, 28th March 2021
Room 2 ClassB
Moderator 1 Dr. med. Vet. Hevy Wihadmadyatami, DVM, M.Sc
Moderator 2 Atika Marwa, DVM
Cohost1 Makruf Arif, DVM, M.Sc
CoHost2 Warih Nugrahani, DVM
Meeting Link http://ugm.id/icavessB
Meeting ID 820 5389 9239
Passcode ClassB
No. Time Paper Title Name Presenter

ID ID
1 13.00-13.08 38-013 Diversity of Macro invertebrate as an Evaluation Pranatasari B-001

of Surface Water Pollution Index on Various Land Susanti
Uses in Upstream Bengawan Solo Watershed
2 13.09-13.16 38-015 Infanticide in Javan Slow Loris: A Case Study in Pangda B-002
Captivity Sopha
Sushadi
3 13.17-13.24 38-024 The Biological Variation, the Index of Yeremia YS B-003

Individuality, and the Reference Change Value for
Haematological and Blood Chemical Analytes in
Ferrets (Mustela putorius furo)
4 13.32-13.40 38-027 The Proboscis monkey conservation in Suwi Tri Atmoko B-004
River, East Kalimantan, Indonesia
5 13.41-13.49 38-037 Ex-situ Exploration of Cemani Chicken in Balai Tatan B-005

Penelitian Ternak (Balitnak), Bogor-West Java Kostaman
6 13.50-13.58 38-043 Diversity of Echinoderms in Intertidal Zone of Salma Dewi B-006

Krakal Beach, Gunungkidul, Yogyakarta Pratita
7 14.06-14.14 38-053 The Locomotion Study of Bekantan (Nasalis Hery B-007

larvatus) at Curiak Island, South, Kalimantan Wijayanto
8 14.15-14.23 38-063 Micromorphology Digestive Tract of imor Lizard Eman B-008

(Varanus timorensis) Kausyn
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9 14.24-14.32 38-064 Histochemical Study of The Esophagus and Mahfud B-009
Stomach of Water Monitor Lizard (Varanus Mahfud
salvator)
10 14.40-14.48 38-070 Potential freshwater crab Parathelpusa convexa Wahyu Tri B-010
(De Man, 1879), as an exotic pet Jayanto
11 14.49-14.57 38-071 Antibiotic Resistance from Different Locality of Yenny Niken B-011
Wild Captured Coura amboinensis Larasati
12 14.58-15.06 38-074 Ichthyodiversity of Nglambor Beach’s Intertidal Rizka Apriani B-012

Zone and Coastal Area, Gunung Kidul, Putri
Yogyakarta
13 15.14-15.22 38-076 The Anatomy of Sunda Porcupine (Hystrix Dian Bekti B-013
javanica) Adrenal Gland Hadi
Masithoh
14 15.23-15.31 38-086 Correlation Between Foot Circumference and Marcel B-014

Body Length, Shoulder Height, and Pelvic Width Wirabrata
in Sumatran Elephants (Elephas Maximus
Sumatranus)
15 15.32-15.40 38-092 Dynamics of Diversity Decapods in the Intertidal Wahyu B-015

Zone at Porok Beach, Gunungkidul, Special Febriani
Region of Yogyakarta, Indonesia
15.48-15.50 Closing
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Room 3 ClassC
Moderator 1 Deny Setyo Wibowo, DVM
Moderator 2 Khesara Sastrin PN, DVM, M.Sc
Cohost1 Yonathan Alvin Sihotang, DVM
CoHost2 Anastasia Diva, DVM
Meeting Link http://ugm.id/icavessC
Meeting ID 953 6909 9650
Passcode ClassC
Paper Presenter
No. Time Title Name
ID ID
Cadmium Levels in Muscle Tissue of Three

Maman
1 13.00-13.08 38-006 Commercial Fish Species Caught from Jakarta C-001
Rumanta
Bay, Indonesia
Identification of Physical Properties, Nutritional Trisnawati

2 13.09-13.16 38-012 C-002
Content and Sensory Arab Chicken and Senskub Wayan
Antimicrobial resistance profile of local isolates

3 13.17-13.24 38-016 Tati Ariyanti C-003
Escherichia coli O157: H7 in Cattle
Widya
The inbreeding case of Gembrong goat at Tumbu
4 13.32-13.40 38-021 Pintaka Bayu C-004
village, Indonesia
Putra
Supplementation of Herbals on the Production

Novita
5 13.41-13.49 38-030 Performance and Gastrointestinal Health in Layer C-005
Ardiarini
Hens
Detection and Testing of Antibiotic Resistance of

Teuku Reza
6 13.50-13.58 38-033 Escherichia coli O157:H7 Isolated from Aceh C-006
Ferasyi
Cattle, Farm Workers, and Water Resources
Macroscopic Pathomorphology of Intravitality

the Slaughter Incision Wound: Reconstructing
7 14.06-14.14 38-036 Eva Yulianti C-007
the Case of "yesterday's dead chicken" through
Veterinary Forensic Pathology Analysis
21
Akhmad
Effect of Transportation on Blood Hematologic
8 14.15-14.23 38-066 Abror As C-008
Profile of Local Indonesian Sheep
Sidiqi
Supply Chain and Potential Pathogens in Frog Sugiyono

9 14.24-14.32 38-080 C-009
Meat from Indonesia Saputra
Prediction of Body Weight from Body

10 14.40-14.48 38-083 Alex Ibrahim C-010
Measurements for Female Batur Sheep
Performance of Broiler Chickens Supplemented Yenny Niken

11 14.49-14.57 38-090 C-011
with Porang Glucomannan and Bacillus subtilis Larasati
The Increase of Ruminant Population Based On

Yenny
12 14.58-15.06 38-098 The Potential of Agricultural Waste as Animal C-012
Yusriani
Feed in Aceh Tamiang District
The Tenderness Quality of Sinjai Superior

Kampong Chickens’ Meat by Using Marinated of Azmi
13 15.14-15.22 38-108 C-013
Pineapple Rind Powder at Different Marinated Mangalisu
Time
The Effect of Antibiotic Growth Promoters (AGP)

Marla
14 15.23-15.31 38-110 on Antibiotic Resistance and the Digestive C-014
Anggita
System of Broiler Chicken in Sleman, Yogyakarta
The Study of Pregnancy Rates (PR), Service per

Conception (S/C), Calving Rate (CR) and the
Agung
15 15.32-15.40 38-114 Incidence of Dystocia in Peranakan Ongole (PO) C-015
Budiyanto
Cows That Inseminated by Various of Frozen
Semen Breeds
15.48-15.50 Closing
22
Room 4 ClassD
Moderator 1 M. Th. Khrisdiana Putri, DVM, M.P, Ph.D
Moderator 2 Andre Firmansyah, DVM
Cohost1 Arvendi Rachma Jadi, M.Sc
CoHost2 Aryo Rais, S.K.H
Meeting Link http://ugm.id/icavessD
Meeting ID 858 2869 4411
Passcode ClassD
Paper Presenter
No. Time ID Title Name ID
Virus Isolation from Culex tritaeniorhynchus, Astri Nur

1 13.00-13.08 38-011 the Primary Vector of Japanese Encephalitis, D-001
Faizah
Using NIID-CTR Cell Lines
Dyah
2 13.09-13.16 38-017 Detection of Babesiosis in Cattle and Buffalo Haryuningtyas D-002
Using duplex PCR and Thin Blood smear Sawitri
Polymorphism of CSN1S1 (g.12164G>A) and Anneke

3 13.17-13.24 38-025 CSN2 (g.8913 C>A) Genes in Pure and Cross D-003
Anggraeni
Dairy Goats
Exploration of Growth Family (GH, GHRH and

Anneke
4 13.32-13.40 38-026 PIT-1) Genes Polymorphisms of Local Swamp D-004
Buffalo for Productivity Improvement in Anggraeni
North Tapanuli Regency, North Sumatra
The Antimicrobial Activity of leaf and fruit Patricius
5 13.41-13.49 38-028 crude extract of Calabash (Crescentia cujete D-005
Atmodjo
L)
Lalu
6 13.50-13.58 38-042 Potential of Polylactic-Co-Glycolic Acid (PLGA) Unsunnidhal D-006
for Delivery DNA Vaccine Model pEGFP-C1 Unsunnidhal
Novita
7 14.06-14.14 38-044 Microorganism on Leather Defects: producing D-007
protease industry in the future Kurniawati
Identification of IGF1 and MSTN genes of Rony Marsyal

8 14.15-14.23 38-052 Lakor Goat from Lakor island, Southwest D-008
Kunda
Maluku Regency encoded growth trait
23
Ruby
9 14.24-14.32 38-056 Antagonistic Activity of Marine Bacteria from D-009
Karimun Island, Indonesia Setiawan
Molecular study of bone morphogenetic

10 14.40-14.48 38-060 protein-15 (BMP-15) gene in Sumba Ongole Cynthia Gaina D-010
cattle, Sumba island
Evaluation Nutrient Content of Liquid Organic Rachmad
11 14.49-14.57 38-034 Fertilizer of Rabbit’s (Oryctolagus cuniculus)
Dharmawan E-001
Urine Fermented by Various Bio-activator
Siti Fatimah
12 14.58-15.06 38-051 Potential of goat manure as organic fertilizer
in North Sumatera Batubara E-002
Tahara Dilla
13 15.14-15.22 38-029 Phytochemical Compound and Acute Toxicity F-001
Test of Carica papaya Leaves Santi
The Effect of Colistin Administration as

Aisah Nurul
14 15.23-15.31 38-084 Medicated Feed on Alanine Aminotransferase F-002
and Creatinine Level in Broiler Infected with Fitri
Escherichia coli
Efficacy of Aloe Vera (Aloe barbadensis) Allan Jeffrey
15 15.32-15.40 38-112 Extracts as Extender for Cryopreservation of F-003
Francia
Sheep (Ovis aries) Semen
15.48-15.50 Closing
24
Room 5 ClassE
Moderator 1 Dinar Arifianto, DVM, M.Sc
Moderator 2 Alfarisa Nururrozi, DVM, M.Sc
Cohost1 Topas Wicaksono Priyo Jr. DVM, M.Sc
CoHost2 Qolby Aghna, SKH
Meeting Link http://ugm.id/icavessE
Meeting ID 889 5599 1151
Passcode ClassE
Paper Presenter
No. Time ID Title Name ID
The Effect of Daun Wungu [Graptophyllum

1 13.00-13.08 pictum (L.) Griff] Ethanol Extract on Listijani
38-022 G1-001
Glucose Tolerance and Pancreas Islets of Suhargo
Langerhans in Ovariectomized Mice
Antimicrobial Resistance in Coagulase
2 13.09-13.16 Negative Staphylococci Isolated from Widodo
38-023 G1-002
Subclinical Mastitis in Ettawa Crossbred Suwito
Goat (PE) in Yogyakarta, Indonesia
Review: Canine Dermatophytosis in
3 13.17-13.24 38-031 Indonesia, a Tropical Country, Compared Yun Fen Tan G1-003
to the Non-tropical Countries
Fish Disease Identification of Fresh Water Yuli

4 13.32-13.40 38-035 fish in the Yogyakarta Special Region, Purwandari G1-004
Indonesia Kristianingrum
A Publication of Escherichia coli in Drh. Lailatul

5 13.41-13.49 38-038 Veterinary Medicine : Insights from Maghfiroh G1-005
Scientometric Analysis (2011-2020)
Comparison of Guinea Pigs Health Raised Laksmindra
6 13.50-13.58 38-039 in Uncontrolled and Controlled G1-006
Fitria
Environments
Mapping The Plague Focus Area with

7 14.06-14.14 38-040 Temporal-spatial Analysis Based on The Rian Saputra G1-007
Body Flea Index in Boyolali District
25
Case Report: Treatment of Feline Indarjulianto
8 14.15-14.23 38-041 Panleukopenia, Otitis and Helminthiasis in Soedarmanto G1-008
a Cat
The Effects Of Natural Aromatase Blockers
9 14.24-14.32 On The Testicle Weight, Size Of Wattle Pudji Astuti
38-048 G1-009
And Histopatological Of Testis In Bangkok
Chicken
Case Report: Diagnosis and Treatment of Indarjulianto

10 14.40-14.48 38-054
Enteritis Caused by Bacterial in a Dog Soedarmanto G1-010
A Review: The Prevalence of Indarjulianto

11 14.49-14.57 38-055 Dermatophytosis on Cats in Indonesia and Soedarmanto G1-011
Turkey
12 14.58-15.06 Moderate Intensity Activity As An Anti- Aditya Candra G1-012
38-058
Fatigue Agent
Neutrophil and Lymphocyte (N/L) Ratio of

Gestation and Lactation Sapera Goats at Anggitya
13 15.14-15.22 38-059 G1-013
Kambing Farm, Sukoharjo, Ngaglik, Nareswari
Sleman
Marchie
14 15.23-15.31 Effect of PGF2α Dosage on Estrus Astrid Da
38-075 G1-014
Synchronization in Sows Costa
Ultrasonography in Leopard Gecko Febri Rizki

15 15.32-15.40 38-085
(Eublepharis macularius) Abdurahman G1-015
15.48-15.50 Closing
26
Room 6 ClassF
Moderator 1 Aria Ika Septana, DVM, M.VPH.
Moderator 2 Antasiswa Windraningtyas R, M.Sc, DVM, M.Sc
Cohost1 Reza Pratama Putra. DVM, M.Sc
CoHost2 Yosua Kristian Adi, DVM, M.Sc
Meeting Link http://ugm.id/icavessF
Meeting ID 899 2522 5643
Passcode ClassF
Paper
No. Time ID Title Name Presenter ID
First Report for Isolation and

1 13.00-13.08 Identification of Avirulent Strain of Indarjulianto G2-001
38-062
Bacillus anthracis from Environmental Soedarmanto
Samples in Central Java, Indonesia
Dian Bekti
The Distribution and
2 13.09-13.16 38-078 Mucopolysaccharides Type in Sunda Hadi G2-003
Porcupine (Hystrix javanica) Placenta Masithoh
Duration of Imunity Against Leptospirosis Ambar

3 13.17-13.24 38-107 and Rabies Commercial Vaccination in G2-004
Retnowati
Working Dogs
Molecular characterization and

4 13.32-13.40 phylogenetic analysis of TevAT1 gene in Moh Mirza G2-005
38-109
Trypanosoma evansi isolated from Nuryady
buffaloes in Ngawi district, Indonesia
Molecular Identification of Trypanosoma Lintang
5 13.41-13.49 38-117 sp. Derived from Cat in Yogyakarta Using G2-006
Firdausy
The Internal Transcriber Spacer-1 (ITS-1)
Detection of Spirometra spp. in Edible Regina
6 13.50-13.58 38-133 Frogs Sold at Camiling, Mayantoc, and G2-007
Villarama
Santa Ignacia Public Markets
Characterization Of Gen Coding Fusion

7 14.06-14.14 Of Newcastle Disease Virus Infected Natif
38-139 G2-008
Chicken (Gallus gallus domesticus) in Maha Kirana
Surabaya
27
Survey of Pet Owners’ Knowledge,
Attitude and Perception on Antimicrobial
8 14.15-14.23 38-141 Resistance and Compliance to Antibiotic Arifah Abdul G2-009
Prescription in West Coast Division of Kadir
Sabah, Malaysia
Ni Luh Putu
Subchronic Toxicity of New Medicine
9 14.24-14.32 38-144 Candidate (+)-2,2’-Epicytoskyrin A for Rischa G2-010
Mice Phadmacanty
The Effect of Heat and Disinfectants on Harimurti

10 14.40-14.48 38-149 G2-011
Infectious Bursal Disease Virus Nuradji
The Effect Of Commercial Detergents On Harimurti

11 14.49-14.57 38-150 G2-012
Newcastle Disease Virus Nuradji
Case Report: Isosporiasis in a Domestic Dodik

12 14.58-15.06 38-151 G2-013
Cat Prasetyo
Mucin heterogeneity in biliary tract of Woro Danur

13 15.14-15.22 38-152 hamster and mouse models for G2-014
Wendo
Opisthorchis viverrini infection
Isolation, Identification of Bacteria from
Vulva Swab of African Pygmy Hedgehog
14 15.23-15.31 38-153 (Atelerix albiventris) and Javanica Anindya Dwi G2-015
Porcupine (Hystrix javanica) and Ash-Santri
Antimicrobial Susceptibility Test
15.40-15.50 Closing
28
DAY 2: Monday, 29th March 2021
Room 1 ClassG
Moderator 1 Khesara Sastrin PN, DVM, M.Sc
Moderator 2 Deny Setyo Wibowo, DVM
Cohost1 Dwi Sunu, DVM, MSc
CoHost2 Amanda Y, SKH
Meeting Link http://ugm.id/icavessG
Meeting ID 833 2761 6214
Passcode ClassG
Presenter
No. Time Paper ID Title Name
ID
Release ratio and mating

competitiveness of sterile male Culex
1 13.00-13.08 38-106 quinquefasciatus (Culicidae): Preliminary A-011
Tri
test of filariasis vector control using Ramadhani
sterile insect technique
Seroprevalence of Japanese Encephalitis Muhammad
2 13.09-13.16 38-111 Virus in Pigs and Cows in Tangerang Umar Riandi A-012
Regency
Tripanosomiasis in Rats in Banjarnegara
3 13.17-13.24 38-116 District and Potential Impact for Public Tri Wijayanti A-013
Health
The Pattern of vector control in malaria Wigati

4 13.32-13.40 38-120
endemic areas of Central Java Province Marsongko A-014
Oral Rabies Vaccination: The New Hope Wahid Fakhri

5 13.41-13.49 38-125 A-015
of Rabies Eradication in Indonesia Husein
Sero-Prevalence Of Johne’s Disease In

6 13.50-13.58 Selected Goat Farms In Tarlac, Lavina Gracia A-016
38-132
Philippines Using Enzyme-Linked Ramirez
Immunosorbent Assay Test
Ecohealth Approach in Anticipating

7 14.06-14.14 38-137 Japanese Encephalitis in Tulungagung Bina Ikawati A-017
District, East Java Province
Case Control Study, Risk Factors and Its Dini
8 14.15-14.23 38-187 Association of Anthrax in South Sulawesi Yudianingtyas A-018
and West Sulawesi Provinces, Indonesia
29
Surveillance of Antimicrobial Dion
9 14.24-14.32 Susceptibility Test on Several Methicillin Adiriesta A-019
38-188
Resistant Staphyloccus aureus derived Dewananda
from Human Medical Cases
Molecular Screening for Chlamydia spp.

10 14.40-14.48 and Salmonella sp. from faecal sample of Marla
38-190 A-020
Sparrows (Passer domesticus) in Anggita
Yogyakarta, Indonesia
Isolation, Identification, and
Antimicrobial Sensitivity Test of Bacteria
11 14.49-14.57 38-154 Isolated from The Rectal Swab of African Astari Kelana G2-016
Pygmy Hedgehog (Atelerix albiventris) Hanindyani
and Javan Porcupine (Hystrix javanica)
Study on Parturisi of Beef Cows in
12 14.58-15.06 38-156 Smallholder Farm: Stages, Placental G2-017
Sri Gustari
Weight, and Placentomes
A Comparison Of Three
13 15.14-15.22 Spectrophotometric Methods For Protein
38-157 G2-018
Concentration Determination Of Soluble Zul Azmi
Trypanosoma Antigen
Identification of Lactobacillus spp. on
14 15.23-15.31 Basis Morphological, Physiological, and
38-161 G2-019
Biochemical Characteristic from Jawa Dini Ludfiani
Super Chicken Excreta
Ocimum sanctum Linn. Ethanolic Extract
15 15.32-15.40 prevents apoptosis on the in-vitro model
38-165 G2-020
neurodegeneration induce by Dinda Aliffia
Thrymethiltin
15.48-15.50 Closing
30
Room 2 ClassH
Moderator 1 Alfarisa Nururrozi, DVM, M.Sc
Moderator 2 Dinar Arifianto, DVM, M.Sc
Cohost1 Makruf Arif, DVM, M.Sc
CoHost2 Warih Nugrahani, DVM
Meeting Link http://ugm.id/icavessH
Meeting ID 864 8550 1587
Passcode ClassH
Presenter
No. Time Paper ID Title Name ID
Species distribution update of Mansonia Sidiq Setyo

1 13.00-13.08 38-101 mosquitoes (Diptera: Culicidae) in Nugroho B-016
Indonesia
Study of Glicoconjugate Distribution on
2 13.09-13.16 38-124 Parotid and Submandibular Glands of Ariana Ariana B-017
Sugar Glider (Petaurus breviceps)
The Differences of Body Measurements
3 13.17-13.24 in Sumatran Elephant (Elephas maximus Deny Setyo B-018
38-134
sumatranus) on Different Captivity Wibowo
Management and Sex
Diversity and Distribution of Mollusca in Yunita Fera

4 13.32-13.40 38-142 the Intertidal Zone of Nglambor Beach, Rahmawati B-019
Gunung Kidul, Yogyakarta
Invertebrate Community Similarity in Yoga Putra

5 13.41-13.49 38-145 B-020
Nglambor Intertidal Zone, Indonesia Aliyani
Morphological Study on the Lingua of Felix Chahya

6 13.50-13.58 38-164 Asian Palm Civet (Parodoxurus Eka Saputra B-021
hermaphroditus)
Morphological Study of the Lingual Golda

7 14.06-14.14 38-167 Papillae of Jentink’s Flying Squirrel B-022
Saragih
(Hylopetes platyurus)
Morphological Study on the Lingua of Angelina
8 14.15-14.23 Domesticated Aonyx cinereus (Asian Kusuma
38-173 B-023
small-clawed otter) in Yogyakarta, Anjani
Indonesia
31
Ayudha
Plumage colours Stability in Inbreed Bahana
9 14.24-14.32 38-175 B-024
Pelung Chicken Ilham
Perdamaian
Gastropod Diversity in Intertidal Zone of

10 14.40-14.48 38-178 Porok Beach, Gunungkidul, Yogyakarta, Lu'lu'ul Aulia B-025
Indonesia
Morphology Study of the Callosciurus Stanislaus
11 14.49-14.57 notatus tongue by Scanning Electron Ivan Davin
38-183 B-026
Microscopy (SEM) and Light Microscopy Pradipta
(LM)
Sialic Acid Glycan Histochemistry in Arvendi
12 14.58-15.06 38-192 Respiratory Mucosa of Common Palm Rachma Jadi B-027
Civet (Paradoxurus hermaphroditus)
The Cytotoxic Activity of Pine Needles Annise

13 15.14-15.22 38-129 Ethanolic Extract of Pinus merkusii on Proboningrat F-004
HeLa Cell Lines
Effects of probiotic and sodium Aria Ika
14 15.23-15.31 38-131 diformate on productivity and immunity F-005
Septana
of broiler chickens
15.40-15.50 Closing
32
Room 3 ClassI
Moderator 1 Atika Marwa, DVM
Moderator 2 Dr. med. Vet. Hevy Wihadmadyatami, DVM, M.Sc
Cohost1 Yonathan Alvin Sihotang, DVM
CoHost2 Anastasia Diva, DVM
Meeting Link http://ugm.id/icavessI
Meeting ID 944 1187 6812
Passcode ClassI
Presenter
Efficiency the Utilization of folow up Rahmi

1 13.00-13.08 38-119 palm plants foor increasing Production C-016
Wahyuni
Beef Cattle in District Pasaman Barat
Erif Maha
2 13.09-13.16 Placenta Expulsion-time on Different Nugraha C-017
38-121
Age and Breed Cows Setyawan
Antibiotic Resistance of Salmonella Sp.

3 13.17-13.24 Isolated From Broilers in Poultry Santi Lestari C-018
38-127
Slaughterhouses in the Special Region of
Yogyakarta
Relationship Between Sheep Farmer’s

Characteristics with the Animal and Alek
4 13.32-13.40 38-135 Environmental Health Management C-019
Ibrahim
Aspects in Batur Village, Banjarnegara,
Indonesia
Farmer and Farm Characteristics
Associations with the Knowledge for Alek
5 13.41-13.49 38-143 C-020
Sheep Estrus Detection in Batur Village, Ibrahim
Banjarnegara, Indonesia
Stress Response of Bali Bulls Subjected Irkham

6 13.50-13.58 38-147
To Shipping Transport Widiyono C-021
Probiotics and Herbs Combination in Thomy

7 14.06-14.14 38-148 Commercial Feed Additives as Growth C-022
Nahak
Promoter in Broiler Chicken
33
Biodiversity Of Local Feeds Availability Anastasia
8 14.15-14.23 38-155 As A Nutritional Source To Increase Bali C-023
Utami
Cattle Productivity In Bangli District, Bali
Effect of candidate feed additive from
combination of Pinus merkusii (jungh. &
de vriese)) and Melaleuca leucadendra Asih
9 14.24-14.32 38-162
(l.) essential oil on kinetic of gas Kurniawati C-024
production and emitted methane from
ruminal fermentation in vitro
Water Holding Capacity of Muscles of Fhaisol Mat

10 14.40-14.48 38-163 Swamp Buffalo Raised with and without C-025
Amin
a Proper Herd Health Program
Evaluation of Sperm DNA Fragmentation Kurniawan

11 14.49-14.57 38-180 in Several Species by TUNEL Assay as an Dwi C-026
Advanced Semen Quality Parameter Prihantoko
Most Probably Number (MPN) of Gilang

12 14.58-15.06 38-195 Escherichia coli in milk from individual C-027
Pandu
and grouped dairy farms
Detection of Oxytetracycline Antibiotic Yatri

13 15.14-15.22 38-196 C-028
Residue in Milk by Bioassay Method Drastini
Chicken Meat Hygienic Standards Aris

14 15.23-15.31 38-045 Evaluation In Traditional Markets Of Purwantoro C-029
Kulon Progo Regency, Indonesia
15.40-15.50 Closing
34
Room 4 ClassJ
Moderator 1 Andre Firmansyah, DVM
Moderator 2 M. Th. Khrisdiana Putri, DVM, M.P, Ph.D
Cohost1 Arvendi Rachma Jadi, M.Sc
CoHost2 Aryo Rais, S.K.H
Meeting Link http://ugm.id/icavessJ
Meeting ID 826 1058 2535
Passcode ClassJ
No. Time Paper ID Title Name Presenter ID
Effect of Biofertilizer and Salinity

Nadhifa
1 13.00-13.08 Stress on Amaranthus tricolor L.
38-067 Athaya D-011
Growth and Total Leaf Chlorophyll
Khairunnisa
Content
Exploration Of Secondary Metabolite
Compounds Of Melon Fruit (Cucumis
2 13.09-13.16 Slamet
38-069 melo L. 'GMP') Extract Using Liquid D-012
Widiyanto
Chromatograph Mass Spectrometry
(Lcms) Method
Full-length 16S rRNA Gene Sequencing
3 13.17-13.24 to Detect Zoonotic Pathogens in Sugiyono
38-082 D-013
Reptiles Using Oxford Nanopore Saputra
MinION
The Development And Optimization Of

Primer Sets Used To Study The
4 13.32-13.40 Herjuno Ari
38-091 Relative Expression Of Androgen D-014
Nugroho
Receptor Gene In Turkey (Melleagris
Gallopavo)
Optimization Of Different Primers In
5 13.41-13.49 The Detection Of Lactoferrin Gene Allen Paul
38-100 D-015
From Different Breeds Of Goats Guillermo
Through Polymerase Chain Reaction
Recombinant Protein Of Sag1 And
6 13.50-13.58 Didik Tulus
38-126 Native Protein For Toxoplasmosis D-016
Subekti
Detection
Restriction fragment length

7 14.06-14.14 Fitrine
38-130 polymorphism (RFLP) analysis genes of D-017
Ekawasti
Toxoplasma gondii using enzym DdeI
35
Muhammad
8 14.15-14.23 The Effectiveness of API® 20NE Kit for
38-140 Ibrahim D-018
Pasteurella multocida Identification
Desem
Toxoplasmosis Molecular Detection
9 14.24-14.32 Using Polymerase Chain Reaction with Aris
38-189 D-019
327 bp Primer Designed Based on Purwantoro
Toxoplasma gondii B1 Gene
evaluation Of The Utilization of GRA7

10 14.40-14.48 Didik Tulus
38-198 & AND SAG1 Genes To Predict D-020
Subekti
Virulence Of Toxoplasma Gondii
Farmers Empowerment through
11 14.49-14.57 Cynthia
38-061 managing organic waste as fertilizer in E-003
Dewi Gaina
Camplong II Village, Kupang Regency
Effects of Biofertilizer Application on
12 14.58-15.06 Growth Rate and Chlorophyll Content Okky Shavira
38-065 E-004
of Spinach (Amaranthus tricolor L.) in Riesty
Salinity Stress Condition
The Utilization Of Agricultural And

13 15.14-15.22 Livestock Waste And The Effect On Damasus
38-068 E-005
New Rice Varieties Yields On Rainfed Riyanto
Rice Field Of Ponjong-Gunungkidul
Sustainability and feasibility
assessments of nanobubble aeration
14 15.23-15.31 38-081 technology in environment-economic- Lily Susanti E-006
socio aspects of Penaeus vannamei
shrimp farming
Utilizing Livestock Manure by
15 15.32-15.40 Anaerobic Fermentation to Produce Aris
38-200 E-007
Organic Fertilizer for Cultivating Low Purwantoro
Glycemic Rice
15.48-15.50 Closing
36
Room 5 ClassK
Moderator 1 Antasiswa Windraningtyas R, M.Sc, DVM, M.Sc
Moderator 2 Aria Ika Septana, DVM, MVPH
Cohost1 Topas Wicaksono Priyo Jr. DVM, M.Sc
CoHost2 Qolby Aghna, SKH
Meeting Link http://ugm.id/icavessK
Meeting ID 870 7124 5944
Passcode ClassK
Presenter
Study of calving interval (CI) and post

1 13.00-13.08 partum estrus (EPP) in beef cattle Wisnu Krisna
38-105 G1-021
based on age, recording status and Nanditya
breed factors
Prevalence of Maedi-visna (Ovine
2 13.09-13.16 Progressive Pneumonia) in Selected
38-113 Lordy Plaza G1-022
Sheep Farms in Tarlac using Enzyme-
Linked Immunosorbent Assay (ELISA)
Level of Alanin Aminotransferase (ALT)
and Aspartate Aminotransferase (AST)
3 13.17-13.24 38-115 Mus musculus Given a Compound of Anggi Pratama G1-023
Temulawak, Turmeric, Kencur, and
Ginger
Comparison of growth, scrotal

4 13.32-13.40 biometry, libido status and semen Mohammed
38-118 G1-024
quality of Malaysian Kedah-Kelantan Sirajul Islam
(KK) and KK ×Brangus bulls at puberty
Effect of Storage Time and
5 13.41-13.49 Temperature on Haematological Dorothea
38-122 G1-025
Parameters of Dogs using The VetScan Megarani
HM5 Analyzer
Diagnosis of Mycoplasma Infection
6 13.50-13.58 Annalie
38-123 Using ImmunoComb Assay in Free G1-026
Paragas
Range Chicken
7 14.06-14.14 Efficiency of Different Natural Fibers as

38-128 Krestel Joy Isla G1-027
an Alternative Non-Absorbable Surgical
37
Suture Material in Rabbit (Oryctolagus
cuniculus)
Bovine Umbilical Vein Endothelial Cells
Gregorius
8 14.15-14.23 Conditioned Medium Prevent Neuronal
38-182 Viktor G1-028
Cell Apoptotic on The In Vitro Model
Lembang
Neurodegeneration
9 14.24-14.32 Investigation of Chlamydophilosis from Madarina
38-185 G1-029
naturally infected cats Wasissa
APPLICATION OF BLOOD COCKLE

10 14.40-14.48 38-193 (Anadara granosa) SHELLS PASTE ON Asfarina, G1-030
RAT BONE FRACTURE Z.M.R
The Effectiveness Of Commercial
11 14.49-14.57 38-197 Bleach And Disinfectants On Bacillus Rahmat Setya G1-031
antrachis Spore Adji
In Vitro Anthelmintic Activity of Clove
12 14.58-15.06 38-136 Leaf Extract (Syzygium aromaticum) Santika F-006
Against Ascaridia galli Anggrahini
Comparative Characteristic Study from

13 15.14-15.22 38-138 Bone Marrow-derived Mesenchymal Medania F-007
Stem Cells Purwaningrum
14 15.23-15.31 Effect of Natural Aromatase Blocker Rizki Fitrawan
38-146 F-008
Towards Adult Pelung Chicken Yuneldi
15.40-15.50 Closing
38
Room 6 ClassL
Moderator 1 Anggi Muhtar P, DVM, M.Sc.
Moderator 2 Putu Cri Devischa G, DVM, M.Sc
Cohost1 Reza Pratama Putra. DVM. M.Sc
CoHost2 Yosua Kristian Adi, DVM, M.Sc
Meeting Link http://ugm.id/icavessL
Meeting ID 849 1040 1432
Passcode ClassL
Presenter
The Effect Of Curcuminoid Temulawak

1 13.00-13.08 Extract In White Rats Which Inducted
38-166 Trini Susmiati G2-021
With Atherogenic Feed Toward Total
Plasma Cholesterol Levels
Antimicrobial Sensitivity Pattern of
Staphylococcus sp, Bacillus cereus,
2 13.09-13.16 Burkholderia mallei, Acinetobacter Marla
38-168 G2-022
calcoaceticus baumanii complex, and Anggita
Nesseria sp Isolated from Dogs with
Dermatitis
Hematology Profile of Equine Non-
3 13.17-13.24 Dinar
38-170 specific Colic Case in Yogyakarta G2-023
Arifianto
Province
The ability of Ocimum sanctum Linn.

4 13.32-13.40 ethanolic extract as A Neuroprotectant Dinda Ayu
38-171 G2-024
on the in vitro model Ramadhani
neurodegenerative (SHSY5Y)
PCR Detection of Avibacterium
5 13.41-13.49 paragallinarum from Layers in Sleman
38-172 Ima Fauziah G2-025
District, Special Region of Yogyakarta,
Indonesia
Association of Canine External Otitis Dion
6 13.50-13.58 38-176 Cytological Findings and Its Causative Adiriesta G2-026
Agents: a Mapping Review Dewananda
7 14.06-14.14 In vitro Anthelminthic Activity of

38-177 Zein Baihaqi G2-027
Aqueous Extract from Sengon Laut
39
(Paraserianthes falcataria) Seeds
Against Haemonchus contortus
Analysis of Blood Draining
8 14.15-14.23 Completeness on Femoral (drumstick) Koekoeh
38-181 G2-028
Broiler Chickens Colorimetric Based by Santoso
Digital Image Processing
Effectiveness Of Povidone Iodine And
9 14.24-14.32 Erif Maha
38-191 Antibiotic Treatment On Postpartum G2-029
Nugraha
Reproductive Disorders In Beef Cattle
The Mice’s Hematological Effect of Christin

10 14.40-14.48 38-194 given the Staphylococcus aureus and Marganingsih G2-030
Persea americana Santosa
Chemotherapeutic Ability of Ocimum
11 14.49-14.57 Ulayatul
38-174 sanctum ethanolic extracton the F-009
Kustiati
Human Lung Adenocarcinoma (A549)
Bovine Umbilical Vein Endothelial Cells
Conditioned Medium (BUVEC-CM) as
12 14.58-15.06 Vinny
38-184 Anti-Inflammatory Agent on the in-vitro F-010
Larasati
Model Neurodegeneration Induced by
TMT
Conditioned Medium of E17 Rat Brain
13 15.07--15.15 Cells Induced Differentiation of Primary Vista
38-199 F-011
Colony of Mice Blastocyst into Neuron- Budiariati
Like Cells
15.20-15.50 Closing
40
41
Acute leptospirosis in working dogs in Jakarta after the flood using
serological and molecular tests
Ambar Retnowati1,*, Agustin Indrawati2 , Upik Kesumawati Hadi2 ,Safika2 ,Uus

Saepuloh3, Susan M.Noor4 and Pratitis S.Wibowo5
1DoctoralStudent of Specialization Medical Microbiology, Health Faculty Veterinary

Medicine, IPB University, Bogor
2Departement of Veterinary Infection Diseases and Veterinary Public Health Faculty
Veterinary Medicine, IPB University, Bogor

3Primate Research Center, IPB University, Bogor
4Indonesian Reseaarch Centre for Veterinary Science,Bogor
5
Jakarta Veterinary Hospital, Jakarta
*Corresponding author: ambarretnowati@yahoo.com
ABSTRACT
Leptospira infections in dogs by serovar Ichterohaemorrhagie and Canicola cause acute

or subacute hepatitis and kidney disorders. Samples of urine, serum and blood were
collected from 4 dogs at the Jakarta animal hospital who showed clinical symptoms of
leptospira during January 2020 after the flood. Only dogs with working dogs that show
symptoms of azotemia with a marked increase in BUN levels and creatinine increases.
Samples collected were tested (Microscopic Aglutination Test-MAT) and (Polymerase
Chain reaction-PCR) and squensing analysis was performed. From the results of the
study showed that average dogs was infected by leptospira interrogran serovar bataviae
in third dogs and one dog was infected by leptospira interrogran serovar
ichterohaemoragica and javanica with the MAT Test. The primary PCR used for Lep Gl (5
'CTG AAT CGC TGT ATA AAA GT) and lep G2 (5' GGA AAA CAA ATG GTC GGA AG). The
resulting amplicon is 285 bp targeting the LIPs60 region gene. Positive PCR test results
were followed by sequencing to see the order of acid base formation. Determination of
Leptospira species by comparing sequences of research results with sequences from
GenBank using the BLAST program (http: //www.ncbi.nlm. Nih.gov/BLAST). The
phylogeny tree was compiled with Mega 7 software
Keywords: leptospira, working dog, MAT, PCR, phylogeny tree
42
Trematode and nematode gastrointestinal infestations in livestock from
different geographical regions in Indonesia
Ekawasti Fitrine1 , Wardhana AH1, Sawitri DH1 and martindah Eny1
1Indonesia Research Center for Veterinary Science
ABSTRACT
Parasitic infestation (Helmhintiasis) remains a major factor which disturbs animal health
and causes huge economic losses in livestock industry of Indonesia, particularly
infestation of both trematodes and nematodes. However, helminthiasis is considered as
a neglected disease by farmers. Investigation of the disease to access its prevalence in
the field are also restricted due to limited financial support. The aim of the study was to
identify the level of trematode (Fasciola sp. and Paramphistomum sp.) and
gastrointestinal nematode (Strongyl) infestations at some farms (cattle, buffaloes,
horses and pigs) from different geographical areas in Indonesia. A total 376 of fecal
samples was collected from some farms located in Malang (Java Island), Banten (Java
Island), Waingapu (Sumba Island) and Denpasar (Bali Island). The fecal samples were
examined using the floating method with saturated NaCl solution (whitlock test) for
nematode and sediment method with methylene blue 1% for trematode investigations.
The data were descriptively analyzed. The results revealed that the percentage of
helminth infestation among examined areas were relatively similar (62.7 – 68.9%) with
various range of trematode and nematode infestations for 7.8 – 58.6% and 18.6 – 68.6%,
respectively. In term of species of helminth infested the livestock, the highest infestation
of Fasciolla sp was detected in cattle raised in Malang (44.1%) and for Paramphistomum
sp. was found in buffaloes raised in Banten (45,00%). In addition, the highest infestation
of Strongyl (nematodes) was disclosed in pig raised in Denpasar (68.6%). Distribution of
helminthiasis prevalence was considerably high at some farms in the examined areas in
Indonesia. Accordingly, helminthiasis control needs to be concerned in order to improve
the livestock productivity.
Keywords: Trematode, nematoda, gastrointestinal, Indonesia
43
Duration of imunity against leptospirosis and rabies commercial
vaccination in working dogs
Ambar Retnowati1, Agustin Indrawati2, Upik Kesumawati Hadi2, Safika2, Susan

M.Noor3
1DoctoralStudent of Specialization Medical Microbiology, Health Faculty Veterinary


3Indonesian Research Centre for Veterinary Science,Bogor
ABSTRACT
Working dogs are dogs that interact very closely with humans so that it is feared that
disease transmission from animals or humans (zoonosis) will occur. Zoonotic diseases
that get attention are Rabies or Leptospirosis. As one of the precautions taken for these
working dogs is a vaccination program. This study aims to determine the level of
protection of dogs against the commercial combo rabies vaccine containing a
combination of rabies leptospirosis, distemper, hepatitis, parvovirus and parainfluenza
carried out in the K9 working dog unit of the Directorate of Customs and Excise, Ministry
of Finance, Lido. The research was conducted on 10 working dogs more than 1 year old.
The examination included observation of post-vaccination symptoms using serum
samples taken after one month, 4 months and 7 months of vaccination against rabies
and leptospirosis. The results of Leptospirosis serological examination using the
Microscopic Agglutination Test (MAT) method showed that the presence of
agglutination on ichterohaemorragie serovars with antibody titers equal to1:100 or
more in the first month with a protection of 70% while the protection was 30% at the
fourth month and 20% at month 1. The seventh month with a titer of 1:100. All samples
did not show agglutination against serovar canicola at 7 months post vaccination but at
1 and 4 months post vaccination showed 10% protective results. In this study also found
a cross reaction against non-vaccine serovars, namely serovar celledoni and javanica.
The test results using Enzyme Linked Immunosorbent Assay (ELISA) against rabies in this
study showed that dogs vaccinated after one month provided 100%, 90% protection for
4 months after vaccination and decreased to 20% after 7 months. This study illustrates
that the study of the duration of immunity to the combo Rabies vaccine in the field is
still above the vaccination coverage in at-risk dog populations and endemic areas. The
emergence of other serovars on leptospirosis agent examination is due to cross-
reactions and it is necessary to watch out for whether there is an increase in titer against
non-vaccine serovars.
Keywords: Immunity, Leptospirosis, Rabies, Vaccination, Dogs
44
A brief discovery of the plerocercoid larvae of spirometra sp. on rice frog
from local traders in bogor
Gloria Animalesto1, Kartika Dewi 1
1Zoology
Division, Research Center for Biology-Indonesian Institute of Sciences (LIPI),
Gedung Widyasatwaloka, Jl. Raya Jakarta-Bogor Km 46, Cibinong, West Java, Indonesia,
16911
ABSTRACT
Plerocercoid larvae of Spirometra tapeworms is the cause of a zoonotic disease named

sparganosis. This research showed the presence of these larvae found in hind leg
muscles of Fejervarya cancrivora and F. limnocharis. All of the hosts were obtained from
local traders in Bogor. A total of 67 rice frogs were collected consisting of 8 F. cancrivora
and 59 F. limnocharis. The results showed that 5 rice frogs (2 F. cancrivora and 3 F.
limnocharis) were infected by Spirometra sp. larvae. Those plerocercoid larvae were
measured, then observed by light and scanning electron microscopes. The detailed
morphology observation clearly showed the anterior extremity, plerocercoid body and
its microtriches.
Keywords: Plerocercoid larvae, Spirometra sp., Rice frog, Bogor
45
Sero-surveillance and potential risk factors associated with avian
influenza subtype h9n2 in backyard poultry in the desert thal of District
Bhakkar Punjab Pakistan
Saifur Rehman1 Mamoona Chaudhry2 Fedik Abdul Rantam1* Mustofa Helmi

Effendi1
1Laboratory of virology and immunology division of microbiology, Faculty of

Veterinary Medicine Universitas Airlangga, Surabaya Indonesia
2. Department of Epidemiology and Public Health University of Veterinary and Animal
Sciences Lahore, Pakistan

*Corresponding author: fedik-a-r@fkh.unair.ac.id
Abstract
Avian influenza is an important zoonotic disease of poultry and mammals including

humans. AIV subtype H9 is consider endemic in Pakistan. This study was aimed to
determine the seroprevalence and potential risk factors associated with transmission of
avian influenza infection in backyard poultry A cross-sectional study was conducted from
December 2018 to May 2019 in desert areas of four tehsil of district Bhakkar. After
proper restraining, disinfect the venipuncture site and 3ml blood was collected from the
brachial vein by using a hypodermic needle from unvaccinated, and health backyard
poultry. A complete predesigned questionnaire was filled in written form.
Haemagglutination-inhibition test was used for the detection of serum antibodies. Chi-
square test of independence and logistic regression were performed using SPSS
software. Out of the 240 samples 105 were positive from all four tehsil. The overall
seroprevalence was 43%. The mean antibody titer of four different tehsil was following
86, 44, 26, and 38 respectively. The presence of dog and cat in the home showed a
significant association with seropositivity of H9 having P-value, 0.026, and 0.043
respectively. AIV subtype H9 has high seroprevalence in the backyard poultry of deserts
thal of district Bhakkar Pakistan. To reduce the prevalence preventive measure such as
biosecurity and monitoring should be improved.
Keywords: Backyard Poultry, Bhakkar, Desert Thal, Pakistan, Seroprevalence.
46
African Swine Fever (ASF) : threat of extinction to nias local pig farm
JonathanAnugrahLase1, VerikaArmansyahMendrofa 2, DianLestari2, and

NovitaArdiarini1
1Balai Pengkajian Teknologi Pertanian (BPTP) Maluku Utara, Sofifi, Indonesia

2Departemen Ilmu Produksi dan Teknologi Peternakan, IPB University, Bogor,
Indonesia
3 Universitas Muhammadiyah Kotabumi, Lampung, Indonesia
ABSTRACT
African Swine Fever (ASF) is a disease that infects pigs. it is known that ASF is caused by
a virus that belongs to the Asfarvididae family and has 23 ASFV genotypes. Common
symptoms caused by ASF attacks on pigs are bleeding in the feces and ears as well as
sudden weakness of livestock, unable to stand, and death of livestock. ASF virus is not
zoonotic so it does not affect human health. This virus has entered Indonesian territory,
including the Nias archipelago, where most of the people cultivate local pigs. The ASF
outbreak on local pigs in Nias resulted in the death of many pigs. This paper discusses
the spread and death of local pigs due to ASF attacks in Nias. Data compiled from the
field and the Animal Husbandry Office in 2020 in the Nias islands, it is known that the
pigs that died due to the ASF outbreak reached 120,570 heads. Meanwhile, the fact
found are that the handling of infected pigs cannot be done optimally, because until
now, effective treatment and vaccination for the treatment and prevention of ASF
infection has not been found. Therefore, in order to prevent the spread of the plague
from becoming more widespread, the current methods that can be applied are the
application of biosecurity in the cattle sheds, isolation of infected livestock and the role
of the government in regulating the entry and exit route for pigs in the Nias region.
Keywords: ASF, Virus, Local Pig, Nias
47
Detection and genetic variation of leptospira sp. in cattle from the
slaughterhouse of Kediri and Yogyakarta
Tito Suprayoga1, Kurniasih Kurniasih2
1Faculty
of Veterinary Medicine, University of Gadjah Mada, Jl. Fauna No. 2,
Karangmalang, Yogyakarta 55281, Indonesia
2Departement of Pathology, Faculty of Veterinary Medicine, University of Gadjah
Mada, Jl. Fauna No.2, Karangmalang, Yogyakarta 55281, Indonesia
ABSTRACT
Leptospirosis is a zoonotic disease caused by Leptospira sp. whose has reservoirs rat,
dog, pig, cattle, and goat. The information about Leptospira species/serovar which
infects cattle in Indonesia is limited, makes it urgent to identify species or serovar
existing to improve prevention and control strategy of leptospirosis. Total sample was
28 cattle, 18 Yogyakarta and 10 Kediri. Serological MAT was carried out on 15 Yogyakarta
cattle serum which reacted with antigen Leptospira sp. serovar bangkinang,
grippotyphosa, icterohaemorrhagiae, canicola, pyrogenes, hardjo, hebdomadis,
pomona, djasiman, robinsoni, bataviae, mini, sarmin, manhao and rama, and showed
that 5 cows from Yogyakarta were seropositive to Leptospira interrogans serovar
Bangkinang. Kidney from 15 Yogyakarta cattle and 10 Kediri cattle were evaluated using
PCR targeting the LipL32 gene, showed 5 cattles from Yogyakarta and 1 cattle from Kediri
were positively infected with Leptospira interrogans. Positive kidney samples infected
with Leptospira sp. were Gram-stained, showed 2 kidneys from Yogyakarta and 1 kidney
from Kediri were infected with Leptospira sp. with rod-shaped stained purple in the
tubule lumen. Interstitial nephritis, perivasculitis, glomerulonephritis, nephrosis and
fibrosis were observed in cattle kidney organs that were positively infected with
Leptospira interrogans. Can be concluded that serologically and molecularly 33% (5/15)
of leptospirosis in cattle in RPH Yogyakarta was caused by Leptospira interrogans serovar
Bangkinang and molecularly 10% (1/10) of cattle slaughtered in Kediri were infected by
Leptospira interrogans. The genetic variation of Leptospira sp. in cattle between Kediri
and Yogyakarta is very small at 0,2-0,6%.
Keywords: Cattle, Histopathology, Leptospira sp., MAT, PCR.
48
Diversity of rodent species and its potency as the vector on transmitting
rodent borne parasitic disease in households
Nur ‘Aini Herawati1, Sudarmaji2
1Indonesian Legumes and Tuber Crops Research Institute, Jl. Raya Kendalpayak No. 66,
Segaran, Kendalpayak, Kec. Pakisaji, Malang – East Java 65162
2AIAT Yogyakarta
ABSTRACT
Rodent species has been known as the major pest in agricultural sector leading to
economic looses. They are also identified being the vector of several rodent borne
zoonotic diseases. However, a specific study on observing their diversity in households
closed to lowland irrigated agroecosistem which corresponds to its role on distributing
those diseases is very limited. To address this issue, a field study was performed during
the 2019-2020 cropping season in two sites (Yogyakarta and West Java) which
categorized as the rice production center. Trapping was conducted consecutively for 13-
30 days using 65-75 snap traps. Roasted coconut and salty fish was used to attract
rodent from surrounding habitat. We found three rodent species (Rattus argentiventer,
Rattus tanezumi, Bandicota indica) and an insectivore small mammal (Suncus murinus)
from 1,950 trap nights in West Java. On the other hand, by setting of 900 trap nights in
Yogyakarta indicated less diversed rodent with only one species (Rattus tanezumi) and
the same insectivore species found. All captured animals were dissected and observed
for the endoparasites. We obtained two species of parasites were potent to transmit
those parasites to the local people if they do not apply a good health practice.
Keywords: rodent, diversity, trapping, parasite
49
The in-vitro study of potential green tea leaves (camellia sinensis) water
extract as anti avian poxvirus on egg chicken egg
Bambang Sutrisno, R. Wasito, Kurniasih, Sitarina Widyarini, Yuli Purwandari

Kristianingrum, Sugiyono
Departement of Pathology, Faculty of Veterinary Medicine, University of Gadjah Mada
ABSTRACT
The purpose of this study was to determine the antiviral effects of green tea water
extract on avian poxvirus in vitro on embryonated chicken eggs by looking at
macroscopic lesion scores and histopathological examination of chorioallantois
membrane organs. This study used 50 semi SPF embryonated eggs aged 9-11 days which
were randomly divided into 5 groups, namely group A, group B, group C, group D and
group E, each 10 semi SPF embryonated eggs. Group A, eggs inoculated with avianpox
virus in the dose of 4,5x10-3 pock/ml and green tea water extract 5% each by 0.2 cc,
Group B eggs inoculated with avianpox virus in the dose of 4,5x10 -3 pock/ml and green
tea water extract 10% each by 0.2 cc, Group C, eggs inoculated avianpox virus in the
dose of 4,5x10-3 pock/ml and green tea water extract 15% each by 0.2 cc, Group D, eggs
inoculated with avianpox virus in the dose of 4,5x10-3 pock/ml and green tea water
extract 20% each by 0.2 cc and Group E, eggs inoculated with avian poxvirus in the dose
of 4,5x10-3 pock/ml and PBS solution by 0.2 cc (as Control), the implementation of
inoculation is carried out in a sterile laminar flow cabinet. The eggs are put into the
hatching machine, candeling is done every day to see whether the eggs are still alive or
not. Then after the day of 6, all the eggs were opened to see macroscopic lesions in the
form of pock formation on the chorioallantois membrane. The entire chorioallantois
membrane was washed with PBS solution and immediately put into each container
giving 10% formalin buffer, for further histopathological processing. Analysis of the
results using ANOVA statistics with the IBM SPSS version 22 program showed that the
number of pock formations in the groups given a green tea dose of 5% and 10%
decreased, although not significantly different (P> 0.05), whereas in the group given
green tea water extract of 15% and 20% decreased significantly (P <0.05).
Histopathological examination of pock formation showed that the positive control group
of the corioallantois membrane ectodermal layer had thicker thickening than the group
given water extract of green tea. The thickening is due to hypertrophy, epithelial
hyperplasia and experiencing hydropic degeneration in which there is a Bollinger body
that is relatively large compared to the green tea group. Conclusion, the administration
of green tea water extract in various doses of 9-day-old embryonated chicken eggs that
have been infected with avianpox virus can suppress the development of avianpox virus.
Keywords: In vitro study, Potential, Camellia sinensis, avianpox virus, embryonated egg
and suppress
50
Phylogenetic and genetic diversity of pathogenic leptospira from
leptospirosis cases in demak Regency-Central Java and DKI Jakarta
Farida Handayani1, Aldiana Astuti2, Endang Burni3, Zainal Khoirudin3, Arum Sih
Joharina1, Ayu Pradipta1, Dimas B. W. Putro1, Wiwik Trapsilowati1, Aryani Pujiyanti1,
Esti Rahardiningtyas1, Arief Mulyono1, Tika Fiona Sari1, Arief Nugroho1, M.Choirul
Hidajat1, Bernadus Yuliadi1, Rendro Wiyanto1, Nurhidayati1, Ristiyanto1, Muhammad
Hussein Gasem4, Tri Wibawa2
1Institute for Vector and Reservoir Control Research and Development (IVRCRD)/Balai
Besar Penelitian Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga, National Institute
of Health Research and Development (NIHRD), The Ministry of Health Republic of
Indonesia
2Department of Microbiology, Faculty of Medicine, Public Health and Nursing, Gadjah
Mada University, Yogyakarta, Indonesia

3Zoonoses Sub-Directorate, the Directorate General of Disease Prevention and Control,
the Ministry of Health, Republic of Indonesia

4Dr. Kariadi Hospital, Semarang, Indonesia / Center for Tropical and Infectious Diseases
(CENTRID), Faculty of Medicine, Diponegoro University, Semarang, Indonesia

*Corresponding author: twibawa@ugm.ac.id
ABSTRACT
Leptospirosis is a worldwide zoonotic diseases, caused by pathogenic leptospires that

infect animals and humans in varying severity. The genetic variations and diversity of the
pathogens contribute to the disease manifestation. One of the tools to study the
molecular epidemiology and evolutionary as well as the population genetics is by
Multilocus Sequence Typing (MLST) methods. The method is the most widespread
genotyping methodology for pathogenic Leptospira. The aim of this study was to
investigate the genetic variation of pathogenic Leptospira in patients from Demak
Regency, Central Java and DKI Jakarta. 108 archived samples from Demak and Jakarta
had been used. The genetic variation was identified by phylogenetic analysis and MLST
method. The analysis genetic variation was performed using MEGA 7 and SEAVIEW4
software. The results showed that from 108 samples we analyzed, 3 samples were
positive in six different genes and indicated that samples from Demak related to L.
interrogans and L. kirschneri meanwhile sample from Jakarta were in different clusters
with Leptospira isolates but related to Leptospira interrogans based on LipL32 gene.
There were several genetic variations of pathogenic leptospira circulates in Demak and
Jakarta which were correspond to L. interrogans, L. kirschneri and L. borgpetersenii.
Keywords: Leptospirosis, pathogenic Leptospira, genetic variations, phylogenetic, MLST
51
Release ratio and mating competitiveness of sterile male Culex
quinquefasciatus (Culicidae): preliminary test of filariasis vector control
using sterile insect technique
Tri Ramadhani1, Upik Kesumawati Hadi2, Susi Soviana2, Tri Wijayanti1, and Asnan
Prastawa1
1Banjarnegara of Health Research and Development, e-mail: 3rdhani@gmail.com

2Entomology Laboratory, Department of Parasitology and Medical Entomology, Faculty
of Veterinary Medicine – Bogor Agricultural University, Dramaga-Bogor, Jl Agatis
Kampus IPB Darmaga, Bogor
ABSTRACT
Sterile Insect Technique (SIT) was an alternative to integrated vector control in filariasis
endemic area. Before applied to the field need to be tested first on a laboratory test.
Irradiation effects will reduce the competitiveness of mating and this can be overcome
by increasing the ratio of sterile male mosquitoes effectively and efficiently. In
laboratory test used the size of cage 30cmx30cmx30cm to test the competitiveness of
male mosquitoes sterile with normal males, different ratios are 1:1; .3:1 and 5:1. Sterile
male mosquitoes were found able to compete with unerirized males to marry normal
females. There is a significantly different ratio of males irradiated and non irradiated to
mating competitiveness. The higher the male ratio irradiation the higher the
competitiveness of the mating. The results of this laboratory test can be used as a basis
for further tests on semi-field and limited field
Keywords: competitiveness mating, SIT, Culex quinquefasciatus, filariasis
52
Seroprevalence of Japanese encephalitis virus in pigs and cows in
Tangerang Regency
Muhammad Umar Riandi1 , Mara Ipa1 , Tri Wahono1, Joni Hendri1, Subangkit2, and
Triwibono Ambargarjito3
1Loka Litbang Kesehatan Pangandaran, Jalan Raya Pangandaran Km. 3, West Java,
Indonesia
2Research and Development Center for Biomedical and Basic Health Technology,
National Insitute of Health Research and Development, Jakarta, Indonesia

3Center for Disease Vector and Reservoir Research and Development Salatiga, Central
Java, Indonesia
ABSTRACT
Japanese encephalitis is a zoonotic vector-borne disease in humans and animals in the

Asia Pacific. Although the number of cases of JE in humans is low, Indonesia is considered
an endemic area for JE. The purpose of this study was to evaluate the seroprevalence of
JE in pigs and cattle in Tangerang Regency as the area with the largest pig population in
Banten Province. There were 101 samples of pig serum and 110 samples of cow serum
from several farms during June - July 2019. Characteristics of management and the farm
environment were recorded for further descriptive analysis. All serum samples were
tested by JEV Specific IgG ELISA and the seroprevalence results were 6.38% in pigs and
7.44% in cows. The follow-up PCR test for porcine and bovine serum found none of the
samples with the JE virus. Based on seroprevalence results and livestock management
characteristics, we suspect that the JE antibody in reactive pig serum was inherited from
their sow before imported from outside the Tangerang district. We suggest the need for
JE virus control in breeding farms in the area of origin of pigs that enter the Tangerang
Regency area, considering that the introduction of the virus can occur through the
transportation of sows or piglets between regions.
Keywords: Japanese encephalitis, seroprevalence, pig, cattle
53
Tripanosomiasis in rats in Banjarnegara District and potential impact for
public health
Tri Wijayanti1 Novia Tri Astuti2, Nuri Alfino Qur’ana3, Jarohman Raharjo4, Bina
Ikawati5, Tri Ramadhani6
1-6 BanjarnegaraHealth Research and Development Unit, National Institute of Health

Research and Development, Ministry of Health Republic of Indonesia
ABSTRACT
Trypanosomiasis is a zoonotic disease caused by Trypanosoma sp. a protozoan parasite

that has flagellum. It has the potential to cause emerging diseases. Generally,
Trypanosoma infection is caused by T. evansi which causes Surra disease and T. cruzi
which causes Chagas disease. Trypanosoma lewisi has been considered a natural
protozoan in mice, not pathogenic to humans but in recent years it has been reported
in humans. This study aims to analyze trypanosomiasis in mice. Research was an
observational with a descriptive approach, conducted in Banjarnegara Banjarnegara on
July - December 2020. Samples were rat’s blood that caught on rats survey in main
market of Banjarnegara District. Blood samples were made thin smear then they were
stained with Giemsa. Microscopically examination with magnification of 1000x used to
get Trypanosoma. There were 157 rats caught during the study consisting of 131 Rattus
norvegicus (brown rats) and 26 R. tanezumi. Totally, 28 rats (16,57%) were positive
Trypanosoma lewisi. Trypanosomiasis in R. norvegicus was 18.3% and R. tanezumi
15.38%. Therefore, there is a need to increase the awareness of these diseases'
transmission.
Keywords: Trypanosoma, rats, Banjarnegara District, Rattus norvegicus, Rattus

tanezumi
54
The pattern of vector control in malaria endemic areas of Central Java
Province
Wigati, R.A.1*, Sidiq Setyo Nugroho2, Anggi Septia Irawan3, Triwibowo AG4
1,2,3,4 The Institute for Disease Vector and Reservoir Research and Development,
NIHRD, Salatiga
*Corresponding author : ajeng0102@gmail.com
ABSTRACT.
In Indonesia, malaria is still a public health problem.To guarantee malaria cases remain
low and not increase again, also to support malaria elimination programme, a research
on the pattern of malaria vector control in malaria endemic areas of Central Java
Province has been conducted from August to December 2016, through searching reports
and articles of malaria vector control. Malaria eradication efforts were carried out not
only the curative treatment, but also preventive action, by controlling of vector. Malaria
vector control in Central Java Province from 2011 to 2015, including; insecticide-treated
mosquito nets, larvae fish spreading, repellent use, indoor residual spraying, and
larviciding. There were several obstacles of malaria vector control, namely: increasing a
number of breeding places, population movements from malaria endemic areas, a
presence of imported malaria cases, geographic condition areas, mindset and behavior
of people who have not been supportive, policy differences in governance, and
insufficient control budgets. Malaria is always present in Central Java Province, because
of differences in vector control programme, mode of control, time and budget. Malaria
vector control policies depend on legislation, local regulation, cross-sectoral budget,
also support and contribution.
Keywords: pattern, vector control, malaria endemic area
55
Oral rabies vaccination: the new hope of rabies eradication in Indonesia
Pebi Purwo Suseno1, Wahid Fakhri Husein2,3,4, Yuni Yupiana1, Andri Jatikusumah2,
Ahmad Gozali2, Ratmoko Eko Saputro2, Arif Wicaksono1, Farida Camelia Zenal2, and
Fadjar Sumping Tjatur Rasa1
1Directorateof Animal Health, Directorate General of Livestock and Animal Health

Services, Ministry of Agriculture, Jakarta, Indonesia
2Emergency Center for Transboundary Animal Diseases, Food and Agriculture
Organization of the United Nations, Jakarta, Indonesia

3Institute of Tropical Medicine, Antwerp, Belgium
4University of Pretoria, Pretoria, South Africa
ABSTRACT
Rabies is endemic in Indonesia with 26 of 34 provinces are infected. Although certain

islands are historically free of dog rabies, newly infected areas have emerged in the last
15 years: Bali, Nias, Larat, and Sumbawa. Mass dog vaccination (MDV) has been chosen
to be main measure to control rabies in Indonesia and has been demonstrated to be
effective in reducing rabies cases in canine and humans in Bali. However, delivering
vaccination in free-roaming dog population is a big challenge. In addition, turnover of
dog population potentially risks the maintenance of vaccination coverage. Oral rabies
vaccination (ORV) has been proved successful in achieving high vaccination coverage in
free roaming dog populations in India, Philippines, and Thailand during field study as
well as eradicating fox mediated rabies in Western Europe. A combination of ORV for
unowned free-roaming dogs and existing MDV practices in confined dog population is a
promising option to eradicate rabies in Indonesia. Here, we discuss about the
perspectives regarding the role of ORV on rabies control and eradication programmes,
as well as factors need to be considered related to socio-cultural characteristics for ORV
implementation. We also provide recommendations on the use of ORV on field level.
Keywords: rabies, free-roaming dog, oral rabies vaccination, mass dog vaccination.
56
Sero-prevalence of johne’s disease in selected goat farms in tarlac,
philippines using enzyme-linked immunosorbent
Harold R. Suba1, Lordy P. Plaza2, Charlie D. Lacayanga2 and Lavina Gracia M. Ramirez1
1JuniorResearcher, College of Veterinary Medicine, Tarlac Agricultural University,

Camiling, Tarlac, Philippines 2306
2Faculty Researcher, College of Veterinary Medicine, Tarlac Agricultural University,
Camiling, Tarlac, Philippines 2306
ABSTRACT
The study was conducted to determine the seroprevalence of Johne’s Disease in

selected goat farms in Tarlac, Philippines using Enzyme-Link Immunosorbent Assay
(ELISA). This also aimed to establish a baseline record for Mycobacterium
paratuberculosis in the herd and to relate the results of ELISA test to the descriptions of
physical examination. A total of 93 goats from the two selected farms were used
regardless of age, sex and breed. The blood samples were collected and brought to the
Tarlac Agricultural University Artificial Insemination Laboratory for antibody testing
using IDEXX MAP Antibody test kit. Interpretation of results is based on the standard
procedure provided by the manufacturer of the antibody test kit. The obtained results
were analyzed using percentage analysis of descriptive statistics. Of the 93 goat samples,
91 (98%) tested positive and two (2%) was resulted to false positive of antibodies against
Mycobacterium tuberculosis. The false positive subjects exhibited coughing, colds,
diarrhea and both are emaciated while the negative animals were all apparently healthy.
Hence, the seroprevalence of Mycobacterium tuberculosis among goat population in
selected goat farms from Tarlac is 2% which was interpreted as suspected.
Keywords: ELISA, Johne’s Disease, Mycobacterium paratuberculosis, Goat,

Seroprevalence
57
Ecohealth approach in anticipating japanese encephalitis in Tulungagung
District, East Java Province
Bina Ikawati1 , Tri Wijayanti1, Dyah Widiastuti1 , Nova Pramestuti1, Tri Isnani1 ,and Tri
Ramadhani1
1Banjarnegara Health Research and Development Unit, National Institute of Health

Research and Development, Ministry of Health Republic of Indonesia Selamanik St
Num 16 A
ABSTRACT
Japanese-B-encephalitis (JE) is a disease caused by the Japanese encephalitis virus from

the Flavivirus group, the Flaviviridae family. In Indonesia, JE cases have not been widely
reported in both animals and humans, although encephalitis in humans is common.
Yamanaka reported positive for anti-JE antibodies in 96 (6%) pigs in Tulungagung
District. Environmental elements need to be identified to support the establishment of
a healthy ecosystem to minimize the potential of JE transmission. This study aims to
identify the environment that can support JE transmission and conceptualize possible
approaches. The method is carried out by conducting environmental observations of
risky environmental conditions in April-November 2016 and searching for supporting
literature. The results showed that there were many puddles in the environment of
chicken and pig farms. Besides that, there were many open puddles of household waste
in the residential areas. This place is the habitat for Culex Sp. mosquitoes. Another
research reported 19.05% of pigs who were positive for Ab JE in Tulungagung District.
Environmental improvement can be done by cultivating fish that is resistant to dirty
water, for example, catfish in ponds in chicken and pig farms and the construction of
closed disposal facilities.
Keywords: ecohealth, Japanese encephalitis, Tulungagung, environment, pig
58
Case control study, risk factors and its association of anthrax in South
Sulawesi and West Sulawesi Provinces, Indonesia
Dini Wahyu Yudianingtyas1,2, Bambang Sumiarto3 , Heru Susetya3, Mo Salman4 and

Risman Mangidi2
1Graduate Student of Veterinary Science, Faculty of Veterinary Medicine, Universitas

Gadjah Mada, Yogyakarta, Indonesia
2Disease Investigation Center Maros, South Sulawesi, Indonesia
3Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas

4Animal Population Health Institute, College of Veterinary Medicine and Biomedical
Science, Colorado State University, Fort Collins, United State of America
ABSTRACT
Anthrax is one of strategic zoonotic disease in Sulawesi island, particularly in South

Sulawesi and West Sulawesi Provinces. Anthrax became endemic disease and spread
into most of districts/ cities in South Sulawesi Province and first outbreak in West
Sulawesi occured in 2016. This retrospective case control study identified and defined
potential risk factors associated to anthrax outbreak in South Sulawesi and West
Sulawesi Province. Anthrax cases confirmation data were obtained from Disease
Investigation Center Maros database during period 2014 – 2020. A matched case control
study design was used to identified potential risk factors (sociodemographics, host,
management, and environment predictor variables) using semi structure questionnaire.
Odds ratios (OR) were used to determine the association between the disease and the
variables studied. Total 224 farms, 75 case farms and 145 control farms from eight
districts/ city were enrolled in this study. Factors that associated to anthrax outbreak
were animal source/ replacement, vaccination, farming system, feeding system,
drinking water system, disposal system, and season.Study findings will be presented to
livestock officer, stake holder decision maker for better prevention and control
strategies toward anthrax disease occurences
Keywords: anthrax, case - control, risk - factor, Sulawesi
59
Surveillance of antimicrobial susceptibility test on several methicillin
resistant staphyloccus aureus derived from human medical cases
Dewananda Dion Adiriesta1, Wasissa Madarina1, Lestari Fajar Budi2, Putra Andani Eka3
and Salasia Siti Isrina Oktavia1
1 Universitas
Gadjah Mada, College of Veterinary Medicine, Clinical Pathology
Department, 55201, Yogyakarta, Indonesia
2Chulalongkorn University, Inter-Department of Biomedical Sciences, Bangkok,
Thailand
3Universitas Andalas, Universitas Andalas Hospital, College of Medicine, Padang,
Indonesia
ABSTRACT
Staphylocccus aureus is a Gram positive bacteria which causes pneumonia, mastitis,

meningitis, urinary tract infections and skin infection diseases. The emergence of
Methicillin Resistant Staphylococcus aureus (MRSA) as highly pathogenic strain in both
veterinary and human medicine is currently a growing global problem. The aim of this
research is to determine the type of antibiotic that are appropriate and effective in
treating diseases caused by MRSA. The susceptibility test was carried out by the diffusion
method using Mueller Hinton Agar (MHA) and against 6 various antibiotic discs. The
samples used for this test were 5 molecularly confirmed Methicillin Resistant
Staphylococcus aureus isolated from human medical cases from Universitas Andalas
Hospital. The results of the measured resistance zone formed were interpreted with a
standard interpretation table of the Kirby-Bauer. In 5 MRSA samples: all of them are
categorically resistant to ampicillin, penicillin, and cefoxitin. Four out of 5 isolates are
also resistant to erythromycin and oxacillin. It was concluded that the only effective
antibiotic used in the experiment againts MRSA was tetracycline.
Keywords: Staphylococcus, antimicrobial resistance, MRSA, Kirby-Bauer Test
60
Molecular screening for chlamydia spp. and salmonella sp. from faecal
sample of sparrows (passer domesticus) in Yogyakarta, Indonesia
Marla Anggita1*, Okti Herawati1, Sidna Artanto1,2
1Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah

Mada, Yogyakarta 55281, Indonesia
2 Department of Infection and Immunology, Faculty of Veterinary Medicine, Utrecht
University, Netherland
ABSTRACT
Wild birds is one of the reservoir agent of some of various zoonotic diseases. The study
was aim to see the potential of sparrow as the reservoir agent of Salmonella sp. and
Chlamydia spp using polymerase chain reaction (PCR) method. We detected the MOMP
gene of Chlamydia spp. and invA gene of Salmonella sp. from faecal sample of sparrows
(Passer domesticus) in local area of Yogyakarta, Indonesia. A total of 30 faecal dropping
samples were collected from sparrows. DNA was extracted from the faecal samples,
then amplified by PCR for the target genes. The amplicons were electrophorized to see
the visualization of DNA on the agarose gel. The result showed the prevalence of the
positive result of Salmonella sp. was 3,3% and the Chlamydia spp. was 70%. The study
indicate that sparrows can spread zoonotic pathogens and this necessitates monitoring
for the epidemiologic status of these pathogens among birds, also applying the
appropriate intervention measures to prevent the transmission of zoonotic diseases
from birds to humans
Keywords: sparrow, Salmonella sp., Chlamydia spp., zoonoses
61
62
Diversity of macro invertebrate as an evaluation of surface water
pollution index on various land uses in upstream bengawan solo
watershed
Pranatasari Dyah Susanti, Nining Wahyuningrum, Rahardyan Nugroho Adi and

Pamungkas Buana Putra 1
1Watershed Management Technology Centre (WMTC)

Jl. A.Yani-Pabelan PO BOX 295 Surakarta 57101
ABSTRACT
Evaluation of the level of surface water pollution is important to determine the health
of an aquatic ecosystem. Diversity of macro invertebrates can be used as a bio-indicator
of the level of water pollution. This study aims to determine the diversity of macro
invertebrates as an evaluation of surface water pollution index in various land uses. The
research method used was a survey by observing macro invertebrates in different land
uses. The observed land uses were forest, paddy field and settlement with 4 replications.
The research location is in the upstream Bengawan Solo watershed, covering 6 sub-
watersheds (Keduang, Nguter, Alang, Upper Solo, Tirtomoyo and Wuryantoro). The
results of the study with the Shannon index showed that the highest diversity index in
forest land use was 2.05 while the lowest in settlements was 1.06. Meanwhile the
highest surface water quality index value in the forest is 3.1 (very clean) while the lowest
is in settlements with a water quality index value of 2 (dirty). Based on the results of
correlation and regression analysis it can be seen the correlation value of macro
invertebrate diversity with a surface water pollution index is 0.778 with the formula Y =
0.967 + 0.954 X.
Keywords: macro invertebrates, diversity, water pollution index
63
Infanticide in Javan slow loris: a case study in captivity
Pangda Sopha Sushadi1*, Wirdateti1 , Raden Taufiq Purna Nugraha1 , Ni Luh Putu
Rischa Phadmacanty1 , and Yulianto1
1IndonesianInstitute of Sciences, Zoology Division-Research Center for Biology, Jln.

Raya Jakarta-Bogor Km.46, Cibinong, Kab.Bogor, 16911, Indonesia
*Corresponding author: pang001@lipi.go.id
ABSTRACT
Over the past decade, the population of Javan slow lorises (Nycticebus javanicus) in
nature has continued to decline. Various conservation efforts by the government and
NGOs have been carried out, but have not yet yielded significant results. Many studies
have highlighted the impact of poaching and forestland conversion as a cause, but the
behavior of N.javanicus in nature, especially mother-infant interactions, is poorly
studied. We investigated the case of a 5-day-old Javan slow loris baby named Monda,
found dead in captivity. Monda was born in captivity and impounded with its mother
and an adult male. She was found dead decapitated with a sign of injury on the right
arm. Microscopic faecal examination of the other lorises showed hair and bone
fragments in the adult male feces. This opens up the possibility of cannibalism in
N.javanicus in nature, which has been encountered but may not be recorded.
Keywords: Nycticebus javanicus, behavior, infant, cannibalism, captivity.
64
The biological variation, the index of individuality, and the reference
change value for haematological and blood chemical analytes in ferrets
(Mustela putorius furo)
Yeremia Yobelanno Sitompul1, Toby Knowles2, and Michelle Barrows3
1Faculty of Veterinary Medicine, University of Nusa Cendana, Kupang City, East Nusa
Tenggara, Indonesia
2School of Veterinary Science, University of Bristol, Langford, Bristol, United Kingdom
3Bristol Zoo Gardens, Clifton, Bristol, United Kingdom
ABSTRACT
Population-based RIs of haematological and blood chemical tests are not sensitive
enough to identify clinically significant changes in individuals when the intraindividual
variation is lower than the interindividual variation. Understanding biological variation
is important before establishing or using currently published reference intervals (RIs) for
comparisons. This is the first study to investigate the biological variation of
haematological and biochemical analytes and calculate the reference change values
(RCVs) in ferrets. This retrospective study analysed blood test results of 13 ferrets that
have been tested every year for seven years. Intraindividual and interindividual
coefficients of variation (CV) for each analyte were calculated using restricted maximum
likelihood (REML) before determining the index of individuality and the RCV. The results
of this study indicate that population-based RIs of MCH, calcium, BUN, ALT, ALP, total
protein, albumin, and globulin had high individuality should be used with caution. It is
still appropriate to use population-based RIs to evaluate RBC count, HGB, HCT, MCV,
MCH, MCHC, WBC count, lymphocytes, monocytes, neutrophils, eosinophils, basophils,
sodium, potassium, calcium, phosphate, glucose, creatinine and amylase.
Keywords: Mustela putorius furo, biological variation, coefficient of variation, index of

individuality, reference change value
65
The proboscis monkey conservation in suwi river, East Kalimantan,
Indonesia
Tri Atmoko*1, 2 and Mukhlisi1
1 Research and Development Institute of Natural Resources Conservation Technology

Jl. Soekarno Hatta Km 38, PO BOX 578, Balikpapan 76112, Indonesia
2 Graduated School of Primatology, IPB University
Jl. Lodaya II, Bogor, West Java, Indonesia

* E-mail: three.atmoko@gmail.com
ABSTRACT
Suwi River was part of Suwi-Mesangat wetland in East Kutai District, East Kalimantan.
This area a habitat of endangered animals, proboscis monkey (Nasalis larvatus), and
siam crocodile (Crocodilus siamensis). The proboscis monkey was an umbrella species in
this area. Unfortunately, several oil palm companies were operated and only remaining
narrow riverbank habitat. Local people have carried out illegal hunting was changed of
proboscis monkey behavior and decreased population. The monkeys more sensitive to
human presence and they are do not use anymore sleeping trees at the first layer from
the riverbank. We estimated approximately 300 monkeys had been hunted until the
year 2013. Nowadays, the habitat is composed of riverine plants species, such as prupuk
(Mallotus sumatranus; IV=121.84%), bungur (Lagerstroemia speciosa; IV=37.10%), and
rengas (Gluta renghas; IV=34.76%). At least 12 monkey groups were distributed in this
area, but the population unknown. The effort of proboscis monkey habitat protection
was made by developing essential ecosystem areas (Kawasan Ekosistem Essential/KEE)
Suwi-Mesangat. Federal and local government, NGO, local community, palm oil
company joined and committed together to protect the KEE Suwi Mesangat area.
Keywords: Proboscis monkey, Suwi River, habitat, vegetation analysis, distributed
66
Ex-situ exploration of cemani chicken in Balai Penelitian Ternak
(BALITNAK), Bogor-West Java
Tatan Kostaman, Soni Sopiyana, Isbandi, and Tiurma Pasaribu
Balai Penelitian Ternak, Jl Veteran III, Bogor 16720, West Java, Indonesia
ABSTRACT
Cemani chicken is the rarest chicken in the world and generally in Indonesia is associated
with medication and ritual ceremonies. The purpose of the research to determine the
performance of Cemani chickens that are kept outside their habitat. A total of 10 hens
kept in litter cages was observed in the exploration. The hens were given commercial
feed and drinking water was given ad libitum. The observed variable was hen day
production (%), first egg weight (g), egg quality, and growth (g). All data obtained were
analyzed descriptively. The results showed that hen day production (%) during 6 months
of production increased, from 14.96% at the beginning of production to 48.97%. The
first egg weight was 31.31 ± 1.43 g with the egg quality is almost the same as the quality
of other local/native chicken eggs. The average body weight of DOC was 25.13 g/head,
8 weeks 839.94 g/head (♂) and 759.98 g/head (♀), and 20 weeks 1537.29 g/head (♂)
and 1455.18 g/head (♀). The observed from the data obtained, the performance of
Cemani chicken in Balitnak showed hen day production, first egg weight, and DOC
weight was slightly lower, but for 20-weeks bodyweight, growth was relatively higher.
Keywords: exploration, ex-situ, performance, Cemani chicken
67
Diversity of echinoderms in intertidal zone of Krakal beach, Gunungkidul,
Yogyakarta
Salma D. Pratita1 , Afifah N. Hidayah1 , Nadya S. Salsabila1 , Amelia R. Alifiah1 ,

Muhammad F Almunawar1 , Safira R. D. Setyasari1 , Vykra A. Firdiana1 , and Rury
Eprilurahman2
1Marine Study Club, Faculty of Biology, Universitas Gadjah Mada Jl. Teknika Selatan,
Sekip Utara, Yogyakarta, 55281, Indonesia
2Laboratorium of Animal Systematics, Faculty of Biology, Universitas Gadjah Mada, Jl.
Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ABSTRACT
Krakal Beach is one of beaches located in Gunungkidul, Yogyakarta. The Krakal Beach
has dominant substrate of dead coral in the intertidal zone. The intertidal characteristics
of Krakal Beach is one suitable structure for echinoderms life. However, research on
diversity of echinoderms in there is lacking. The purpose of this research is to know the
diversity of echinoderms in the intertidal zone of Krakal Beach, Gunungkidul,
Yogyakarta. Research on diversity is quite important because it can be used to determine
the habitat, distribution, and diversity of a species in this location. Sampling was carried
out on November, 22nd 2020. The method used is purposive random sampling method
along the coastal-line in the intertidal zone. The results of this research are identified
and classified in each class. Based on the results, there are seven species from class
Echinoidea, two species from class Ophiuroidea, and one species from class
Holothuroidea. Species from class Echinoidea there are Diadema setosum, Diadema
antillarum, Tripneustes gratilla, Tripneustes ventricosus, Echinotrix calamaris,
Echinometra mathaei, and Stomopneustes variolaris. The species from class
Ophiuroidea there are Ophiocoma scolopendrina, and Ophiocoma erinaceus. While
species from class Holothuroidea is Actynopyga echinities.
Keywords: diversity, echinoderms, Krakal Beach
68
The locomotion study of bekantan (nasalis larvatus) at Curiak Island,
South, Kalimantan
Hery Wijayanto1,*, Rini Widayanti2, Arvendi Rachma Jadi1, Slamet Raharjo 3, Amalia
Rezeki4, Alfian5, Aldy Heryanoor5
1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Jl.

Fauna No. 2, Caturtunggal, Depok, Sleman, Yogyakarta 55281, Indonesia, Tel. +62-274-
560862
2Department of Biochemistry, Faculty of Veterinary Medicine, Universitas Gadjah
Mada. Jl. Fauna No. 2, Caturtunggal, Depok, Sleman, Yogyakarta 55281, Indonesia, Tel.
+62-274-560862,
3Departmenet of Internal Medicine, Universitas Gadjah Mada, Kuningan, Yogyakarta,
55281 Indonesia.
4Sahabat Bekantan Indonesia, South Kalimantan, Indonesia.
5 Biology Study Program, Faculty of Science, Lambung Magkurat University,
Banjarmasin, South Kalimamtan

Corresponding author: herykh@ugm.ac.id
ABSTRACT
Indonesia is home for 62 species of non-human primate (NHP), 5 species out of them
are endemic. One of those endemic species is Bekantan (Nasalis larvatus), distributed in
Kalimantan Island. Bekantan have unique social relationship on the group. The alfa-male
dominancy is related with the nose zise. Bigger nose of male bekantan is more dominant
in the group. Other unique fact of bekantan is their ability in locomotion. From our
previous study reported that bekantan intermembral index is about 90. By such index,
bekantan is hind limb dominant animals. The unique of bekantan extremities is also
found as digital webbing among the finger. The only NHP have digital webbing, which
support their movement in the water. Animals with hind limb dominant are generally
bipedal with clinging and leaping movement ability on the trees. However, our
observation was found that bekantan have all capabilities to move, on the ground and
on the trees they walk not only bipedally, but quadripedally as well. Based on our study,
bekantan also have positive correlation between the lenght of femur with body size.
Unfortunately, during two months observation, we did not catch any bekantan water
activities. We hypotheses they avoid enter the water caused by the water predator. At
Curiak Island, we found there are many monitor lizards around and hunt particularly
infant and juvenile of bekantan.
Keywords: Bekantan, Curiak Island, movement ability
69
Micromorphology digestive tract of timor lizard (varanus timorensis)
Germanus K Franus, Mahfud, Nur R. Adawiyah Mahmud*
Department of Biology Education, Faculty of Teacher Training and Education,

University of Muhammadiyah Kupang
Email: emankausyn@gmail.com
Email: mahfud.aph@gmail.com
*Email: nuradawiyah836@gmail.com
ABSTRACT
The objective of this research is to determine the micromorphology of digestive tract of

endemic lizard in timor island, that known as timor lizard (varanus timorensis). The
sample here is digestive system of adult of v. Timorensis. Varanus timorensis was
anesthetized and perfused by using paraformaldehyde 4% as fixation solution. Then,
followed by making histology preparation and stained with hematoxylin eosin (he). The
observations were made of the wall structures of digestive tract of v. Timorensis. I.e.
Esophagus, stomach, and intestine. The results show that the digestive tract of v.
Timorensis generally consist of four layers, i.e. Mucosa, submucosa, tunica muscularis,
and serosa. The esophagus has a rather long protrusion to lumen. The protrusions in
three parts of esophagus wall have different size. The protrusion in cranial part was
longer and thick than in the esophagus wall in medial and caudal parts. In the stomach,
the mucosa protrusion was larger than in esophagus. The tunica mucosa in the stomach
protrude into the lumen with two different forms, namely, form like straight truncus and
in apical part is widened like fungiform. The intestine of v. Timorensis was divided in six
sections, and microscopic observation in these six parts show the difference in tissue
structure. Histology section in section i of intestine is intermediate channel with small
size and short which was located in the behind of stomach. The tissue structure of
intestine section i have similar structure to pylorus in stomach. Intestine sections ii to v
have same tissue structures that consist of mucosa layer, submucosa, tunica muscularis
and serosa. The mucosa layer was protruding high to lumen of intestine or that
commonly called as villi.
Keywords: varanus timorensis, digestive tract, micromorphology
70
Histochemical study of the esophagus and stomach of
water monitor lizard (Varanus Salvator)
Mahfud1*, Ernawati1, Adi Winarto2 , Nur R. Adawiyah Mahmud1
Department of Biology Education, Faculty of Teacher Training and Education,

University of Muhammadiyah Kupang
3Laboratory of Histology Faculty of Veterinary Medicine, Institute of Agricultural Bogor
Email: mahfud.aph@gmail.com
ABSTRACT
In order to succeed in conservation program of water monitor lizard, the knowledge

about of the biological data, behavior, and habitat suitable were needed. One of the
important data to be studied is data on the digestive tract, especially the histological
data of esophagus and stomach. The materials used in this research are digestive tract
of water monitor lizard, especially esophagus and stomach organs. The animals were
anesthetized, exsanguinated and fixed using paraformaldehyde 4% by perfusion
method. The obtained tissue samples were then prepared for histological evaluation.
Paraffin method with section thickness 3-4 µm then stained using hematoxylin-eosin
(HE) and Casson's Trichrome (MT), alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS).
The results showed that the esophageal mucosal wall was composed by pseudostratified
columnar epithelium, not keratinization. The esophageal mucosa is supported by the
lamina propia, connective tissue and muscle layer. The muscle layer consisted of
longitudinal layers toward the caudal. The stomach wall consists of three layers: mucosal
layer, lining the gland, and muscle layer. Mucosal layer was composed by
pseudostratified columnar epithelium, lining the gland consisted of glandular cells.
Muscle layer consisted of inner circular layers. The result of AB pH 2.5 and PAS staining
showed mucosal epithelium and gastric lumen are secreted high concentration of
neutral mucopolysaccharides and not secreted acidic mucopolysaccharide.
Keywords: digestion, esophagus, stomach, water monitor lizard
71
Potential freshwater crab parathelpusa convexa (de man, 1879), as an
exotic pet
Wahyu Tri Jayanto 1 , Hurul Ein 1 , Luthfia Uswatun Khasanah 1 , Wahyu Febriani1 and
Laksmindra Fitria 2
1Undergraduate student at Departement of Tropical Biology, Faculty of Biology,

Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
2Laboratory of Animal Physiology, Departement of Tropical Biology, Faculty of Biology,
Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ABSTRACT
Crabs have been known inhabiting diverse habitat. Some species can be found in the
sea, while some others living in freshwater, even in land (terrestrial). Some crabs have
unique appearance with complexion or peculiar patterns on their body parts. The aim
of this research was to explore the potential of crabs as exotic pets through the study of
their biological profile. Samples were collected following purposive random sampling
method from December 29 th 2020 to January 21 st, 2021, located in one of rice fields
at Yogyakarta province. Samples were determined based on morphological characters
and their behaviors are then observed for the safety as pets. Result showed that the
sample was identified as Parathelphusa convexa (De Man, 1879). This species is
categorized as data deficient (DD) in conservation status, however we found it a lot in
rice field areas. We consider this species is potential as exotic pet due to its attractive
appearance and docile behaviors.
Keywords: Biological profile, Crab, Exotic pet, Freshwater biodiversity, Morphology
72
Antibiotic resistance from different locality of wild Captured Coura
amboinensis
Okti Herawati1 , Dorothea Vera Megarani2 , Aria Ika Septana3, Nuri Rahma Nuranisa4,
Atma Aulia Rusmonoputri4 , Dian Bekti Hadi Masithoh4 and Riefqy Pradipta Baihaqie5

Mada, Indonesia
2Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Indonesia
3Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Indonesia
4Undergraduate Student, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Indonesia
5Doctoral Student, Program Study Sain Veteriner, Faculty of Veterinary Medicine,

6 West Java Province Animal Hospital
ABSTRACT
Antibiotic resistance is increasing both of human and animals worldwide. The spread of
antibiotic resistant has been reported in natural environment. Turtles have been
proposed as bio-indicator of contaminated effluents. Up to now, a few studies evaluated
the antibiotic resistant flora of Coura amboinensis. This study aim to identify profile of
antibiotic resistance in different locality of Coura amboinensis. This study examined 20
cloacal swab from 4 different locality of Coura amboinensis (Sumatera, Kalimantan,
Sumbawa and Sulawesi). Conventional bacteriological methods led to the isolation of
bacterial commonly as opportunistic pathogens (i.e. Eschericia coli). Bacterial isolates
tested with 8 antibiotics (gentamycin 10μg, colistin 50μg, amoxicillin 3μg, ampicillin
10μg, cloramphenicol 30μg, ciprofloxacin 5 μg, oxitetracycline 30μg dan streptomycin
10μg). The result was analyzed descriptively and showed variations in resistance.
Resistance to colistin was the highest in three locality except Coura ambeinensis locality
Kalimantan. Resistance to Amphicilin was highest in this locality. The presence of
antibiotic resistance in Coura amboinensis indicates that environmental antibiotic
resistance is widespread in bacterial flora obtained from Coura amboinensis.
Keywords: antibiotic, resistance, Coura amboinensis, locality
73
Ichthyodiversity of Nglambor beach’s intertidal zone and coastal area,
Gunung Kidul, Yogyakarta
Rizka Apriani Putri1.* Yunita Fera Rahmawati 1 , and Tatag Bagus Putra Prakarsa 1
1Department of Biology Education, Faculty of Mathematics and Sciences, Universitas

Negeri Yogyakarta
*Corresponding author: rizka_apriani@uny.ac.id
ABSTRACT
Indonesia has been known as one of several countries with high biodiversity. One of the
equatorial ecosystems that rich in diversity other than tropical rain forest is Intertidal
Zone with coastal area as its ecological buffer. Research in intertidal zone and coastal
area diversity has been very limited until recent years. This research aims to study the
fish diversity of intertidal zone and coastal area of Nglambor Beach, one of the beaches
in Gunung Kidul, Yogyakarta with unique ecosystem.This research were done in two
steps which are: survey and specimen samplings followed by species identification and
data analysis. Fish were sampled using combination of catch and release methods and
Underwater Surveillances techniques. Collected specimens will be used in identification
and will be kept as voucher specimens for further investigation. Based on the specimen’s
collection there were 15 fish species belongs to 8 families identified form Intertidal Zone
of Nglambor Beach. Based on data analysis, Nglambor beach’s Intertidal zone and
coastal area are medium in species diversity and high in evenness. While now it’s still
can be considered to be stable in its species diversity and population, prevention
measures still needed to be applied as human activities might intervene and caused
ecosystem imbalance in this area
Keywords: Ichthyodiversity, Fish, Intertidal Zone, Nglambor Beach
74
The anatomy of sunda porcupine (Hystrix javanica) adrenal gland
Masithoh Dian Bekti Hadi1, Padeta Irma2, and Budipitojo Teguh2*
1Student
of Doctoral Program Veterinary Science, Faculty of Veterinary Medicine
Universitas Gadjah Mada, Yogyakarta, Indonesia
2Department of Anatomy, Faculty of Veterinary Medicine Universitas Gadjah Mada,
Corresponding author: budipitojo@ugm.ac.id
ABSTRACT
The Sunda Porcupine (Hystrix javanica) is one of the protected animals in Indonesia. In
fact, the illegal hunting of sunda porcupine increase and the presence is decreasing. On
the other hand, documentation about the anatomical structure of the endocrinological
system of sunda porcupine is very limited. The adrenal glands are organs that produce
various hormones that has important role in the body. This study aims to determine the
structure of sunda porcupine adrenal glands. Adrenal gland samples were taken from
nine Javan sunda porcupines from Tawangmangu. The structure of the adrenal glands
was visualized by Hematoxylin eosin (HE) staining. The results showed that the adrenal
glands were found in the abdominal cavity, small, a pair and located anterior to the
kidney. The adrenal glands have two parts, the medulla and cortex. The adrenal cortex
comprises three zones: zona glomerulosa (outer), zona fasciculata and zona reticularis.
Keywords: Adrenal gland, Hematoxilin Eosin, Sunda Porcupine.
75
Correlation between foot circumference and body length, shoulder
height, and pelvic width in Sumatran Elephants (Elephas Maximus
Sumatranus)
Hery Wijayanto, Deny Setyo Wibowo, Rini Widayanti, Tri Wahyu Pangestiningsih, Woro
Danur Wendo, Machmud Asvan, Anastasia Stephanie Gunawan, Marcel Wirabrata
Correspondence author: herykh@ugm.ac.id
ABSTRACT
Sumatran elephants are large mammals inhabiting most of Sumatra Island and East
Kalimantan. International Union for Conservation of Nature and Natural
Resources (IUCN) has raised Sumatran elephants' status to critically endangered. The
population has been plummeting and extinction could happen in the foreseeable future.
Conservations had been done to minimize the risk of extinction. Elephant’s body
measurement is essential in conservation practices, particularly to perceive their health
status and to determine doses for medications. Considering the size of the elephants,
the mahouts doing the recording can get harmed by the elephants, therefore an easier
method is required to accurately estimate the size of the elephants. This research is
purposed to find correlations between the foot circumference (forefoot and hindfoot)
and body length, shoulder height, pelvic width in Sumatran elephants to help minimize
the risk of the mahouts in recording the body of the elephants by measuring the size of
the elephants based on their foot circumferences or their footprints, also to estimate
the size of adult wild Sumatran elephants using the methods mentioned above. The
research collects data from male and female adult elephants inhabiting numerous
locations in Aceh, Taman Safari Indonesia, Kebun Binatang Gembira Loka, Taman Wisata
Borobudur, and PLG Duri Pekanbaru. Measurements of forefoot and hindfoot
circumference, body length, shoulder height, and pelvic width were done using
standardized methods on elephant measurements referring to The American Zoo and
Aquarium Association Elephant Species Survival Plan and The Elephant Research
Foundation (2006); Kachanapangka et al (2007); Hile et al. (1997). Measurements were
done using modified Vernier callipers and roll meters. Measurement results were then
calculated using correlation and linear regression test.
Results acquired from the forefoot circumference show a very strong correlation with
shoulder height (R=0,809) and strong correlations with body length (R=0,769) and pelvic
width (R=0,73) meanwhile, results acquired from the hindfoot circumference show a
very strong correlation with pelvic width (R=0,826) and strong correlations with body
length (R=0,775) and shoulder height (R=0,747).
Keywords: body measurement, Elephas maximus sumatranus, forefoot circumference,

hindfoot circumference, linier regression test, Sumatran elephant
76
Dynamics of diversity decapods in the intertidal zone at Porok Beach,
Gunungkidul, Special Region of Yogyakarta, Indonesia
Wahyu Febriani1 Amalia Puji Cahyani1 , Auliya Nur Illahi1 , Elzahra Nadya Putri1 ,
Muhammad Arya Sadewo1 , Ratriana Kartikasari1 , Wahyu Tri Jayanto1 , Zahra Nurul
Hana1 ,and Suwarno Hadisusanto 2
1Marine Study Club, Faculty of Biology, Universitas Gadjah Mada

2Laboratory of Ecology and Conservation, Faculty of Biology, Universitas Gadjah Mada
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ABSTRACT
Decapods were member of tropical benthic communities and plays an important role in
the coastal ecosystem. Porok Beach was center research, but rarely scientific reports on
the level of decapods diversity. This research aims to study the composition structure,
level of diversity, uniformity and dominance of Decapods in Porok Beach, as well as its
limiting factors. Sampling was conducted in intertidal zone with purposive random
sampling method. The primary data of the study analyzed the Diversity, the Uniformity
, and the Dominance Index through Microsoft Excell 2019. Data results will be presented
in the form of histograms.The result showed that 10 species from 5 families with 10 total
individuals of decapods on October 17th 2020 and 10 species from 7 families with 23
total individuals of decapods on January 23th 2021. Shannon-Wiener diversity index
value (H’)2-2,3 and the uniformity index value (E) 0,05-0,10, and the dominance index
value (D) 0,002-0,1. Intertidal zone of Porok Beach were rarely visited by tourists so it
has been high diversity and high uniformity, but the index dominance of decapods was
low. Therefore, this study would be a preliminary study for further research and as the
basis for future biodiversity management and conservation.
Keywords: Biodiversity Conservation, Crustaceans, Diversity Index, Dominance Index,

and Uniformity Index.
77
Species distribution update of Mansonia musquitoes (Diptera: Culicidae)
in Indonesia
Sidiq Setyo Nugroho1
1Institute for Vector and Reservoir Control Research and Development, National
Institute of Health Research and Development, Indonesia Ministry of Health.
Hasanudin St. 123, Salatiga City, Central Java, Indonesia. 50721.
ABSTRACT
Mansonia is a genus of mosquitoes of which several species are confirmed vector for
lymphatic filariasis. Many countries are still struggling to achieve lymphatic filariasis
elimination included Indonesia. Knowledge of the Mansonia mosquito diversity and its
distribution is essential in its control efforts. Six of eight Mansonia species in Indonesia
have been confirmed as lymphatic filariasis vectors. This paper aims to provide an
update on the distribution data of the Mansonia mosquito in Indonesia. Species
distribution data were summarized from various literature regarding the Mansonia
mosquito. The data is complemented by the results of National Research on Disease
Vector and Reservoir (Rikhus Vektora) results conducted by the National Institute of
Health Research and Development (NIHRD) in 2015-2018. There are new distribution
records for four species of Mansonia mosquitoes in Indonesia. Mansonia annulata, Ma.
annulifera, and Ma. indiana are now recorded distributed throughout the archipelago.
Meanwhile, Ma. bonneae has a new distribution record in Maluku. The identification
key for female Mansonia mosquitoes in Indonesia is included in this paper.
Keywords: Mansonia, mosquito, distribution.
78
Study of glicoconjugate distribution on parotid and submandibular
glands of sugar glider (Petaurus Breviceps)
Ariana1 *, Teguh Budipitojo1, Irma Padeta 1, Tri Wahyu Pangestiningsih1, Arvendi

Rachma Jadi 1
1Departmen of Anatomy, Faculty of Veterinary Medicine, Universitas Gajah Mada

Corresponding author: ariana@ugm.ac.id
ABSTRACT
Sugar gliders are not classified as insectivorous but are classified as omnivorous
mammals. These wild animals are often used as pets. The distribution of glyconjugates
in the parotid glands and submandibular glands is expected to be the primary data for
the distribution of glyconjugates in the salivarius major glands of other pet wild animals.
This study aims to determine the distribution of glycoconjugates in the parotid and
submandibular glands sugar gliders with the histochemical lectin method. The samples
that have been collected came from two adult male Petaurus breviceps, from
Yogyakarta. The material used is the parotid and submandibular glands. Samples that
have been collected are then fixed in 10% formalin for 24 hours. Samples were
dehydrated, clearing and embedding in paraffin. Paraffin blocks were cut with a
thickness of 8 µm, then the tissue was stained with lectin Concanavalin A (Con A),
Soybean agglutination (SBA), Wheat Germ Agglutinin (WGA). The staining results will be
analyzed qualitatively descriptively on the intensity of lectin reactivity which indicated
by the presence of a brown color in the tissue. The results showed that the parotid
glands of sugar gliders with the histochemical technique of the lectin Con A and WGA
had a negative reaction, thus indicating that the parotid gland adenomer does not
contain glucose and N acetylglucosamine (GlcNac) carbohydrates, whereas SBA lectin
staining had a weak positive reaction, indicating that the parotid gland adenomer
contains N Acetylgalactosamine (GalNac) carbohydrate. Sugar glider submandibular
glands with Con A lectin staining weak positive reaction, thus indicating that the
submandibular gland adenomer contains carbohydrate glucose. Wheat Germ Agglutinin
and SBA lectin staining on submandibular glands were negative reactions, thus indicate
that the submandibular gland adenomer does not contain GlcNac and GalNac
carbohydrates. The parotid glands of sugar gliders contain GalNac carbohydrates
whereas submandibular glands contain carbohydrates glucose.
Keywords: parotid gland, submandibular gland, glycoconjugate, sugar glider, lectin
79
The differences of body measurements in Sumatran elephant (elephas
maximus sumatranus) on different captivity management and sex
Deny Setyo Wibowo1, Alek Ibrahim1, Rini Widayanti2, Wisnu Nurcahyo3, Muhammad
Wahyu4, Johan Michaux5, Hery Wijayanto6
1Veterinary Science Doctoral Program, Faculty of Veterinary Medicine, Universitas

Gadjah Mada, Yogyakarta 55281, Indonesia

3Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta 55281, Indonesia

4Veterinary Society for Sumatran Wildlife Conservation, Medan, Indonesia
5Conservation Genetics Research Unit of University of Liege, Belgium
6Department of anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
ABSTRACT
Sumatran elephants is one of wild animals in Indonesia that currently status is critically
endangered. Conservation problem relates to the capture of wild elephants and located
into Elephant Conservation Centers (EECs) and Zoo. To support and help monitor
management conservation, this study was aimed to measure the body size of Sumatran
elephant between semi-captive (EECs) and full captive (Zoo) management in different
sex. This study was conducted in ECCs in Sumatran island and Zoo in Java island. Body
measurements, including Chest Ghirt (CG), Body Length (BL), Body Height (BH), Tarsal
Circumference (TC) and Carpal Circumference (CC) were collected from a total 57
samples of captive Sumatran elephant (28 samples from EECs and 27 samples from Zoo).
Data were analyzed using a completely randomized factorial design with different
management (semi-captive and full captive) and sex (male and female) as the factors.
The results shown that only TC (75.96 vs 78.66) and CC (74.93 vs 86.79) have significant
result (P<0.05) in differences captivity management. Sex categories (male and female)
show significant result (P<0.05) on CG (323.06 vs 302.63), BL (306.69 vs 279.51), BH
(232.19 vs 210.34) and TC (83.06 vs 75.10). There is no interaction between captive
management and sex on body measurements. In conclusion, there is significant
differences of body size in male and female elephant. Although, there is no significant
results between the management captivity except the TC and CC. This results could be
usefull to evaluate the conservation management based on body size and further
research for Sumatran elephant conservation
Keywords: Body measurement, Captivity, Conservation, Sumatran elephant
80
Diversity and distribution of mollusca in the intertidal zone of Nglambor
Beach, Gunung Kidul, Yogyakarta
Yunita Fera Rahmawati1*, Milade Annisa Muflihaini2, and Yoga Putra Aliyani3
1Department of Biology Education, Faculty of Mathematics and Natural Sciences,

Yogyakarta State University
2Postgraduate of Biology Education, Faculty of Mathematics and Natural Sciences,

3Bachelor of Biology Education, Faculty of Mathematics and Natural Sciences,

Corresponding author: yunita.fr@uny.ac.id
ABSTRACT
The intertidal zone is the area between the highest and lowest tides, which represents
the transition from ocean conditions to land conditions. The area of the intertidal zone
is limited, but has a high diversity of biota, one of which is molluscs. This study
determines the diversity and a distribution of molluscs that exist along the intertidal
zone of Nglambor Beach, between August and November 2020. Observations of all
molluscs, were carried out at two random stations using 10 plots measuring 1 x 1 m2
with a distance of 5 meters. A total of three classes of Mollusca (Gastropoda, Bivalvia
and Malacostraca) belonging to thirteen families and 20 species were found from upper
to lower an intertidal zone. The upper intertidal zone was recorded to have the highest
diversity and an evenness index (Shannon-Wiener a diversity index: H '= 2.524 and Pielou
evenness index: J' = 0.932) compared to the middle and lower zones. It can be concluding
that the diversity index in the study location is categorized as medium and its evenness
is high. Thais hippocastanum is the most dominant species found in the upper and
middle zones, while Thais tissoti dominates in the lower zone.
Keywords: intertidal zone, molluscs, diversity, evenness
81
Invertebrate community similarity in Nglambor intertidal zone,
Indonesia
Yoga Putra Aliyani1 , Yunita Fera Rahmawati1, and Millade Annisa Muflihaini1
1Biology, Faculty of Math and Science, Universitas Negeri Yogyakarta, Colombo 1

Street, Yogyakarta, Indonesia
ABSTRACT
Invertebrate community similarity in Nglambor intertidal zone in Indonesia was

investigated in August and September 2020. The data of Invertebrate and algae were
obtained by plot method. Abiotic factors such as water PH, light intensity, and salinity
were measured at each station. The similarity in invertebrate communities among
habitat types was determined by Dice’s coefficient, meanwhile to estimate similarities
in habitat utilization we use Jaccard’s coefficient in PAST 4 software. Both invertebrate
community similarity and habitat utilization displayed in cluster dendrogram. A total of
5 phyla was recorded, comprising Echinodermata, Annelida, Mollusca, Arthropoda, and
Nemertea. Algae cover varies from each station with the average cover reaches 52%.
The result of cluster analysis showed different patterns in invertebrate community
similarity and habitat utilization
Keywords: Biodiversity, Intertidal Zone, Invertebrate, Nglambor, Similarity
82
Morphological study on the lingua of asian palm civet (Parodoxurus
hermaphroditus)
Felix C.E. Saputra1*, Ignasius G.P. Dharu Wicaksono**, Angelina K. Anjani, Stanislaus
I.D. Pradipta, Dwi Liliek Kusindarta, and Hevi Wihadmadyatami1*
1Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada University,

Yogyakarta Indonesia 55281.
**The authors contribute equally
*corresponding author: heviwihadmadyatami@ugm.ac.id
ABSTRACT
Asian palm civet is a commonly wild animal found in Indonesia with large population
distribution around South East Asia. Asian palm civet is a carnivore mammal which diet
consists of fruits, grains, the variation of peanuts, and small insects (omnivore-like). The
variation of diets makes the digestive organ play a very crucial role, especially the
tongue. We examine the characteristics of the Asian Palm Civet lingua both anatomically
and histologically using Scanning Microscope Electron (SEM) and histochemical staining.
Our data shows on the apex of Asian Palm Civet's lingua found the arrowhead filiform,
conical, and fungiform papillae. In the corpus are found giant-arrowhead filiform, scale-
like filiform, foliate papillae, and fungiform. Meanwhile, in the radix are found three
circumvallate, bluntly conical, conical, and fungiform papillae. Weber gland shown in
posterior radix reacts positively and produces a magenta-red colour from PAS staining.
Keywords: Paradoxurus hermaphroditus, Tongue, Scanning Electron Microscope,

Histochemical stain
83
Morphological Study of the Lingual Papillae of Jentink’s Flying Squirrel
(Hylopetes platyurus)**
Golda Rani Saragih1, Geraldus Gunawan1, Made Bagus Auriva Mataram2, Ulayatul
Kustiati1, Dwi Liliek Kusindarta1, Hevi Wihadmadyatami1*
1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada,

Yogyakarta Indonesia 55281
2 Integrated Laboratory for Research and Testing, Universitas Gadjah Mada,
Yogyakarta, Indonesia 55281

*Corresponding author : heviwihadmadyatami@ugm.ac.id
ABSTRACT
This study describes the anatomy and histology of the tongue in the Jentink’s flying
squirrel (Hylopetes platyurus), a rodent native to Indonesia. We examined the
characteristics of the tongue using scanning electron microscopy (SEM) and light
microscopy (LM). Macroscopic observations revealed three distinct regions: the apex,
corpus, and radix. The dorsal surface of the apex was characterized by a median groove
and lacked a lingual prominence that is common in many rodents. SEM and LM revealed
the distribution of papillae over the dorsal surface of the tongue. The papillae could be
categorized as mechanical papillae (filiform, conical, large conical) and gustatory
papillae (fungiform, foliate, and vallate). Filiform papillae covered the apex and the
anterios two-thirds of the corpus. Fungiform papillae were distributed among the
filiform papillae from the apex to the corpus, whereas the foliate papillae were present
only on the apex. Cocnical papillae were located on the posterior of the corpus, and the
large conical papillae were on the lateral surface of the posterior part of the radix. This
research provides the first detailed observations of the lingual papillae in Hylopetes
platyurus. The shape, structure, and distribution of papillae are described and compared
with those in other rodent species.
Keywords: Hylopetes platyurus, lingual papillae, light microscopy, scanning electron

microscopy
**This work already submitted to Thai Journal of Veterinary Medicine
84
Morphological Study on the Lingua of Domesticated Aonyx cinereus
(Asian small-clawed otter) in Yogyakarta, Indonesia
Angelina Kusuma Anjani1, Stanislaus Ivan Davin Pradipta1, Felix Chahya Eka Saputra1,
Hevi Wihadmadyatami1, and Dwi Liliek Kusindarta1
1Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada

Yogyakarta, Indonesia, 55281
*Corresponding author: indarta@ugm.ac.id
ABSTRACT
This work aimed to observe the morphological dorsal lingual surface of the
domesticated Aonyx cinireus (A. cinireus) or Asian short-clawed otter in Yogyakarta. The
five adult samples of domesticated A.cinireus were obtained from local specialized
breeding without regard to gender. The samples were then acclimated to the laboratory
for one day before the euthanasia procedure—the morphological and SEM analysis
applied to the samples. Macroscopically, the tongue of domesticated A. cinireus divided
become three-part: apex, corpus, and radix. Apex is the shortest part and can move
freely. Meanwhile, the corpus revealed a median groove bent along the corpus. On the
radix typically found the circumvallate papillae and vallecular epiglottis. SEM
observation describes the lingual of A.cinireus consists of mechanic papillae (horny
filiform, leaf-like filiform, bifid filiform, trifid filiform, elongated leaf-like, triangular
filiform papillae, conical papillae), and gustatory papillae (circumvallate and fungiform
papillae). Besides, we also describe von Ebner and weber gland on the apex, corpus, and
radix. AB-PAS staining was applied to identify the gland's characteristics.
Keywords: Aonyx cinereus, histochemistry, lingual papillae, scanning electron

microscope
85
Plumage colours Stability in Inbreed Pelung Chicken
Hendry T.S.G. Saragih1 , Ayudha B.I. Perdamaian2
1Laboratoryof Animal Structure Development, Faculty of Biology, Universitas Gadjah

Mada. Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia
2Gama Chicken Research Team, Laboratory of Genetics and Breeding, Faculty of
Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara
ABSTRACT
Pelung is one of the most importance local chicken in Indonesia. Either genetic
introgression and inbreeding depression were the major threat for local chicken gene
pool. The objective of this research was to investigate the effect of serial inbreeding
mating to plumage colours of Pelung chicken. Pure Pelung chicken which purchased
from Cianjur sub-district was undergo full-sib mating through five generation. All
offspring phenotypes was recorded. The results show gradual plumage phenotype
change of inbreed Pelung chicken. Initially, adult male chicken had Black-red coloured
as wildtype (wt) swifted to partridge and black-silver coloured in later generations.
Otherwise, female chicken plumage changes not obviously seen. The current findings
indicating that inbreeding made recessive traits to be expressed which some of them
might had deleterious effect. Random mating should be maintained to preserve genetics
stability of Pelung chicken.
Keywords: inbreeding depression, plumage colours, Pelung chicken
86
Gastropod Diversity in Intertidal Zone of Porok Beach, Gunungkidul,
Lu’lu’ul Aulia1, Azka Syamila1 , Deby Rossa Amalia1 , Desti Putri Amartia1 , Maria
Bernadeth Tukan1 , Maria Bening Wohingati1, Gaby Cahyadi1, and Rury
Eprilurahman2
1Marine Study Club, Faculty of Biology, Universitas Gadjah Mada Jl. Teknika Selatan,
Sekip Utara, Bulaksumur, Depok, Sleman, D.I.Yogyakarta, 55281, Indonesia
2Laboratory of Animal Systematics, Faculty of Biology, Universitas Gadjah Mada Jl.
Teknika Selatan, Sekip Utara, Bulaksumur, Depok, Sleman, D.I.Yogyakarta, 5528,

Indonesia
ABSTRACT
Porok beach is located in Gunungkidul, Yogyakarta, Indonesia. The substrate of this

beach is dominated by fine sand and death corals with algae, which is suitable as the
habitats of Gastropods. Gastropods are one of the classes in Molluscs and the most
highly diversified classes. This research aimed to study the diversity of gastropods in the
intertidal zone of Porok Beach, Gunungkidul, Yogyakarta. The research was conducted
on October 17th, 2020 in Porok Beach (8.134293° S, 110.557734° E). Data collection was
carried out in the intertidal zone of Porok Beach used purposive random sampling. The
research held when ecological parameters were ± 26oC for water temperature and ± 8
for pH. The result of this research revealed 8 families of Gastropods. The families of
classes Gastropods that found were Aplysiidae, Chitonidae, Columbellidae, Conidae,
Nerita, Trochidae, Turbinidae, Volutidae. The most diverse family in this research was
Conidae with 3 Genera.
Keywords: Diversity, Gastropod, Porok Beach, Conidae
87
Morphology Study of the Callosciurus notatus tongue by Scanning
Electron Microscopy (SEM) and Light Microscopy (LM)
Stanislaus I.D. Pradipta**, Emilia Ika Megawati1**, Ulfah Damia1**, Hevi

Wihadmadyatami1, Dwi Liliek Kusindarta1*

*corresponding author: indarta@ugm.ac.id
** The authors contribute equally
ABSTRACT
Callosciurus notatus is a mammal that belongs to the order Rodentia and the family
Sciuridae. These animals' diet varies, such as fruits, seeds, leaf buds, flowers, bark, and
tree sap. This research aimed to observe morphological identification of the Callosciurus
notatus lingual papillae. The tongue morphology and lingual papillae distribution of
Callosciurus notatus was investigated through two different methods: scanning electron
microscopy (SEM) and light microscopy (LM). Macroscopically, the tongue was divided
into three parts: apex, corpus, and radix. The dorsal surface of the apex occurs median
groove and lateral prominence. SEM and LM observation showed that the apex bears
three types of papillae: leaf-like filiform, bifid filiform, and fungiform. On the corpus,
broad leaf-like filiform papillae, saw-like filiform papillae, bifid filiform papillae, and
fungiform papillae. Meanwhile, on the radix, conical-like filiform papillae, fungiform
papillae, conical papillae were found. The lateral part of radix showed Weber gland that
reacts positively by PAS staining.
Keywords: Callosciurus notatus, lingual papillae, scanning electrone microscope, light

microscopy
88
Sialic Acid Glycan Histochemistry in Respiratory Mucosa of Common
Palm Civet (Paradoxurus hermaphroditus)
Arvendi Rachma Jadi1, Hery Wijayanto1, and Woro Danur Wendo1*

Abstract. Common Palm Civet (Paradoxurus hermaphroditus) can be serve as the

reservoir for SARS-CoV by the attachment of the virus to the spesific glycan containing
receptor. Determination of glycosylated molecules in trachea, bronchus, bronchioles,
and alveolus of common palm civet is necessity. This study used respiratory tracts of
three different sexes of adult civet obtained from previous research. The samples were
collected and produced as paraffin blocks. The blocks were sliced 4 µm thick using a
rotary microtome and samples were histochemical stained. Wheat Germ Aglutinin
(WGA), Maackia Amurensis Lectin II (MAL II), and Sambucus Nigra Agglutinin (SNA) lectin
histochemistry were employed to detect N-acetyl-D-glucosamine and sialic acid (SA) α-
2,3, N-acetylgalactosamine or galactose, respectively. The GalNAc glycan was produced
by trachea epithelial goblet cells and decorated the cilia combine with SA α-2,6Gal but
not SA α-2,3Gal. The cilia of bronchus experienced with weak α-2,6Gal, whereas the
surface epithel of bronchioles and alveolus strongly performed by SA α-2,3Gal. Further
investigation id needed to clarify.
Keywords: common palm civet, lectin, receptor, sialic acid
89
90
Cadmium Levels in Muscle Tissue of Three Commercial Fish Species
Caught from Jakarta Bay, Indonesia
Maman Rumanta1*, Rony Marsyal Kunda2
1Biology
Education, Study Program, Faculty of Education and Teacher Training,
Universitas Terbuka, Jakarta, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Science, Universitas
Pattimura, Ambon, Indonesia
ABSTRACT
Cadmium (Cd) is a toxic heavy metal that accumulates in living systems. Cd has a long
half-life and bio accumulates in plants, invertebrates, and vertebrates. The toxic effects
following exposure include growth retardation and organ systemtoxicity, with kidney
and liver toxicity most reported with in higher organisms. This study objective to find
the concentrations of heavy metal Cd in muscle tissue of Three commercial fish species
caught from Jakarta bay, Indonesia. Three species of marine fish, namely Leiognathus
splendens, Pampus argenteus, and Rastrelliger faughni, were collected directly from
fishermens in the Jakarta bay with each sample three replications (triplo) at two
different seasons (western and eastern seasons). Analysis of the concentration of Cd
levels in the muscle tissue of Three fish species was carried out in the Agro-Based
Industry Calibration and Analytical Laboratories (ABICAL), Bogor, Indonesia. Data of Cd
accumulation on tissue from each fish sample were analyzed based on the t-test and
ANOVA, using SPSS version 22 software. The results of this study showed that Pampus
argenteus had high Cd accumulation in two different seasons compared to Leiognathus
splendens and Rastrelliger faughni. The accumulation of Cd levels in Pampus argenteus
during the eastern season was 0.067 ± 0.040 mg/kg, whereas in western season it was
0.207 ± 0.280 mg/kg and there was no significant difference in results between the two
periods. The accumulation of Cd levels in Leiognathus splendens and Rastrelliger
faughni, was not detected during the eastern season, but was detected in the weastern
season by 0.030 ± 0.010 mg/kg for Leiognathus splendens, and 0.110 ± 0.056 mg/kg for
Rastrelliger faughni. Based on the results of this study, it was concluded that Cd levels
in muscle tissue from Three commercial fish species caught from Jakarta Bay, Indonesia
did not exceed the maximum value set by New Zealand FDR, FAO and SNI. 7387.2009,
but it needs attention because the accumulation of Cd heavy metals in the muscle tissue
in fish causes a serious health problem in the human body that consumes it.
Keywords: Cadmium, Muscle Tissue, Fish, Jakarta Bay
91
IDENTIFICATION OF PHYSICAL PROPERTIES, NUTRITIONAL CONTENT AND
SENSORY ARAB CHICKEN AND SENKUB
Wayan Trisnawati, Nyoman Suyasa, Anastasia Sischa Jati U dan

Ni Ketut Ari Tantri Yanthi
Bali Institute of Agricultural Technology Assessment (BPTP)

By Pass Ngurah Rai Pesanggran Street Denpasar, Phone (0361) 720498
Correspondent Writer : wayan_trisna@yahoo.co.id
ABSTRACT
Arab and Senkub chickens are superior types of free-range chickens, the difference is
Arab chickens type of laying hens while Senkub chickens include slaughtered chickens
with a cut age of 2 months. The purpose of this study was to determine the physical
characteristics, nutritional and sensory content of Arab chicken and Senkub compared
to Broiler chicken meat. Meat quality research was carried out at the Bali Institute of
Agriculture Technology Assessment Laboratory. The best quality chicken meat based on
physical quality and nutrient content is found in Senkub chicken meat, with cooking
losses at 30.73%; texture of 1.37 kg/cm2; pH of 5.75; protein by 17.09%; fat by 3.68%.
Panelist preferences for the three types of chicken received almost the same assessment
of color, texture and level of preference. But the panelist's preference for taste has a
marked difference between Broiler chicken and Arab chicken and Senkub chicken.
Broiler and Arab chicken meat scored 4.50-4.60 (slightly savory to savory) and Senkub
chicken meat scored 3.60 (not savory to savory).
Keywords: Chicken meat, identification, physical, chemical and sensory
92
Antimicrobial resistance profile of local isolates Escherichia coli O157:
H7 in Cattle
Tati Ariyanti dan Faidah Rachmawati

1Balai Besar Penelitian Veteriner, PO Box 151, Bogor 16114
tatiariyanti@gmail.com
ABSTRACT
Escherichia coli O157: H7 is one of the foodborne pathogens that can be transmitted to
humans through direct contact with infected cattle or through contaminated food of
animal origin. Calves (calf) infected by E. coli O157H7 show symptoms of mild diarrhea
until bloody diarrhea. The antibiotics is a common therapy and has been chosen to treat
diarrhea calf. However, it is now known that the E. coli strain has been resistant to many
antibiotics. The purpose of this study was to determine the resistance profile of 15 types
of antibiotics in 62 isolates of E. coli O157H7 from cattle and one isolate of E. coli
O157H7 ATCC 43984 as a control. Antibiotic sensitivity tests are carried out using agar
diffusion techniques as recommended by the Clinical and Laboratory Standards Institute
(2015). Test results on 62 local isolates of E. coli O157H7 showed that 75.81% were
resistant to Cephalotin, 69.35% were resistant to Sulphamethoxazole, 35.48% were
resistant to Ampicillin, 25.81% were resistant to Amoxicillin/Clavulanic Acid, 14.52%
resistant to Nalidixic Acid, 12.90% resistant to Streptomycin, 12.90% resistant to
Kanamycin, 8.07% resistant to Tetracycline, 8.07% resistant to Trimethoprim, 4.84%
resistant to Gentamycin, 4.84 % resistant to Ciprofloxacin, 3.23% isolates resistant to
Norfloxacin, Enrofloxacin and Amoxicillin. While E. coli O157H7 ATCC 43984 isolate was
resistant only to Sulphamethoxazole antibiotics, intermediates to Cephalotin and
Streptomycin and were still sensitive to 12 antibiotics. The study also found 79.03%
isolates resistant to 1-5 types of antibiotics, 4.84% resistant to 6-10 types of antibiotics
and 4.84% resistant to 11-15 types of antibiotics. Based on the results of the study, it
can be concluded that there are variations of multi-drug resistance in the local isolates
of E. coli O157: H7 tested. Therefore it is necessary to supervise the use of antibiotics in
cattle on farms so that the occurrence of multidrug antibiotic resistance can be
controlled and the transfer of antibiotic-resistant foodborne pathogens to humans can
be prevented.
Key Words: resistance, antibiotic, Escherichia coli O157H7, cattle, diarrhea
93
The Inbreeding Case of Gembrong Goat at Tumbu Village, Indonesia
Muhammad Ridho1 , and Widya Pintaka Bayu Putra2,*

1Faculty of Agriculture, Sebelas Maret University, 57126 Surakarta, Indonesia
2Research Center for Biotechnology, Indonesian Institute of Science, 16911, Cibinong,
Indonesia
ABSTRACT
Gembrong goat is one of Indonesian germ plasm that originated from Bali Province.
This goat is very rare with highly of inbreeding level. This reseach was carried out to
estimate the inbreeding coefficient (Fx) and inbreeding rate values of Gembrong goat
at Tumbu village, Bali province, Indonesia. The pedigree records of goat at year 2015
were obtained from previous study through literature study. Research showed that the
Fx value in Gembrong goat at Tumbu village was 0.413 and consisted of high category
(Fx > 0.125). Meanwhile, the inbreeding rate (IR) in Gembrong population at Tumbu
village was 0.025 and consisted of high category (IR > 0.020). It was concluded that
highly of Fx and IR values in Gembrong goat was caused by less number of population.
In the future, the recording system for Gembrong goat is important for reduce the
inbreeding level.
Keywords: Gembrong goat, inbreeding, Tumbu village
94
Supplementation of Herbals on the Production Performance and
Gastrointestinal Health in Layers Hens
N Ardiarini1 and J A Lase1

1North Maluku Assesment Institute for Agricultural Technology, Ministry of Agriculture,
Indonesia
ABSTRACT
This study conducted to determine the effect of herbals on the production performance
and gastrointestinal health in layers hens. This study used 50 layer hens that were
assigned randomly into two different types of diets. There are basal diet (T0) and basal
diet+3ml herbals/1000g ration (T1). Each treatment was repeated 5 times with the
number of chickens/replication was 5 chick. The herbals that supplemented into chicken
feed consisted of tumeric, sambiloto leaves, soursoup leaves, ginger and lemongrass. T-
test results showed that supplementation of herbals affect the production performance
(P>0.05). Gastrointestinal health which shown by the histomorphology of villous height,
vilous width, crypth depth, and the number of goblet cells in herbals treatment (T1) were
higher than those in basal diet (T0). It can be concluded that herbals supplementation
affect the productions performance and gastrointestinal health of layer hens.
Keywords :Herbals, Gastrointestinal, Layer hens, Production performance
95
Detection and Testing of Antibiotic Resistance of Escherichia coli
O157:H7 Isolated from Aceh Cattle, Farm Workers, and Water Resources
Teuku R. Ferasyi1,4,*, Mudhita Zikrullah Ritonga1,4, Ismail Ismail1,4, Azhari Azhari1,4,

Andi Novita1,4, Rastina Rastina1,4, Nurliana Nurliana1,4, Wahyu Eka Sari 1,4, Safika
Safika2, Vierman Vierman3, Rina Aulia Barus3, Nellita Mutia3, Maryulia Dewi1, Teuku
Shaddiq Rosa4, Rezky Ramadhan4, and Aulia Malik Fajar4.
1Faculty of Veterinary Medicine, Universitas Syiah Kuala, 23111 Banda Aceh, Indonesia
2Faculty of Veterinary Medicine of IPB University, Bogor, Indonesia.
3Livestock Breeding and Fodder Centre (BPTU-HPT), Indrapuri, Aceh Besar.
4Centre for Tropical Veterinary Studies-One Health Collaboration Center of Universitas
Syiah Kuala, Banda Aceh
ABSTRACT
A study based on one health concept was conducted for detection and testing antibiotic
resistance of E. coli O157:H7 bacteria from several sources in an aceh cattle breeding
farm location. A total of 28 isolates that found positive E. coli contamination from cattle
faecal swab samples (18), hand swab of farm workers (9), and water samples (1) in the
study location were analysed for identification of strain E. coli O157:H7 and then
continued for testing antibiotic resistance. Molecular analysis using RT-PCR method was
conducted for identification of strain E. coli O157:H7. Then, antibiotic resistance profiles
was testing by the Kirby-Bauer disk diffusion method according to Clinical and
Laboratory Standards Institute guidelines. The results of molecular analysis showed that
16 isolates from faecal swab, 8 isolates from hand workers swab, and 1 water samples
were confirmed as E. coli O157:H7. The antibiotic resistance test showed that 25 (100%)
E. coli O157:H7 isolates were resistant to streptomycin, Clindamycin, and Erytromycin.
Then, 1 isolate (4%) of E. coli O157:H7 was resistant to Chloramphenicol, and the rest
were susceptible. On the other hand, none of isolates resistance to Gentamycin, but at
11 (44%) and 14 (55%) of them were intermediately resistant and susceptible,
respectively.
Keywords: aceh cattle, faecal swab, environment, E. coli O157:H7, resistance, one
health
96
Macroscopic Pathomorphology of Intravitality the Slaughter Incision Wound:
Reconstructing the Case of "yesterday's dead chicken" through Veterinary Forensic
Pathology Analysis
Eva Yulianti1, Yuli Purwandari K2 , and Yudha Nurhantari3
1Master's Student in Veterinary Science, Faculty of Veterinary Medicine, Gadjah Mada

University, Yogyakarta, Indonesia
2Faculty of Veterinary Medicine, Pathology Department, Gadjah Mada University,
3 Faculty of Medicine, Forensic and Medicolegal Department, Gadjah Mada University,
ABSTRACT
Slaughter incision wound is evidence of chicken halal slaughter that used as pathological
objects for forensic activities at criminal case of “Yesterday Dead Chicken” in Indonesia.
In this research, the macroscopic pathomorphology of intravitality determinated from
wound’s rubor-tumor reaction and difference in length of the wound before and after
closed in five slaughtering time intervals: 0.5, 1, 2, 4 hour after dead and alive broilers.
For all time intervals in all parameters tested are not significant (0.401; 0.390; 0.096).
These results attributed by the rapid death of chickens, where the homeostatic
processes of injury have not yet seen clinically. Elasticity value from difference in length
of wound was not significant up to 4 hours postmortem were also associated with skin
anatomy on the chicken’s neck which was thinner and looser than other parts even
though have undergone rigormotis phase, as well as the collagen content which affected
the delayed autolysis process in the skin. It could be concluded that that macroscopic
pathomorphology cannot be distinguished between the halal chicken and “yesterday
dead chicken”. Microscopic intravitality examination by histopathological methods is
needed to reconstruct the time of the injury in this case.
Keywords: Macroscopic, Intravitality, Slaughter Incision Wound,”Yesterday Dead

Chicken”
97
Effect of Transportation on Blood Hematologic Profile of Local
Indonesian Sheep
Sugi Winarsih1, Akhmad Abror As Sidiqi2, , and Sarmin2
1 DinasPertanian, Pangan, dan Perikanan Kabupaten Sleman, Jl. Dr. Rajimin Sucen,
Tridadi, Sleman, Daerah Istimewa Yogyakarta, Indonesia
2 Universitas Gadjah Mada, Faculty of Veterinary Medicine, Jl. Fauna No. 2,
Karangmalang, Sleman, Daerah Istimewa Yogyakarta, Indonesia
ABSTRACT
Animal transportation is an important aspect of livestock animal distribution. On the
other hand, transportation had been known causing stress and affecting animal
physiology. The objective of this research was to investigate effect of transportation on
hematologic profile of local Indonesian sheep. Blood sample was collected via jugular
vein from three sheep before and after transportation. The sample was analysed
haematologically to gain hematologic profile. The hematologic parameters included
erythrocyte, MCV, MCHC, haemoglobin, and haematocrit. There was no significant
difference (P>0.05) among hematologic profile of sheep before and after transportation.
This study showed that transportation did not affected hematologic profile of local
Indonesian sheep.
Keywords: hematologic profile, local Indonesian sheep, transportation
98
Supply Chain and Potential Pathogens in Frog Meat from Indonesia
Sugiyono Saputra1 , Yuli S. Fitriana2, Anik B. Dharmayanthi2, Amir Hamidy2
1Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

2Museum Zoologicum Bogoriense, Research Center for Biology, Indonesian Institute of
Sciences
ABSTRACT
Indonesia is the largest exporter of frog meat and the major source of more than two
third supply globally. Most of the frog meat are harvested from the wild and processed
in home industries before collected by exporter companies. In this study, we aimed to
describe supply chain of frog meat and to obtain bacterial isolates associated with frog
meat by performing sampling in the processing facility and frozen frog leg products,
using Salmonella Isolation Transwab® and Listeria Isolation Transwab®. Presumptive
positive result was indicated by colour change in the medium from brown to black.
Bacterial isolation was performed by inoculating enriched samples into xylose lysine
deoxycholate agar (XLD agar) to detect Salmonella colonies and into horse blood agar to
observe hemolytic colonies. In total, Salmonella colonies was detected in 40% (16/40)
of swab samples from all seven exporters while presumptive Listeria was detected in
75% swab samples (15/20). The 16S rRNA bacterial identification reveals that collected
isolates were belonging to genus Salmonella, Macrococcus, Klebsiella, Citrobacter and
Chryseobacterium. Since the presence of Salmonella and other potential enteric
pathogens in frozen frog legs may pose an infection risk, we suggest that each processing
facilities should implement sanitation quality assurance as well as microbiological
testing for their products to prevent the spread of foodborne pathogens.
Keywords: Frog legs, Salmonella, Listeria, foodborne pathogens, 16S rRNA.
99
Prediction of Body Weight from Body Measurements for Female Batur
Sheep
Alek Ibrahim1, Ridwan Yuniawan2, Bayu Andri Atmoko2, Wayan Tunas Artama1, I
Gede Suparta Budisatria2, and Rini Widayanti1
1Facultyof Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2,

2Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna No.3, Karangmalang,
ABSTRACT
Body weight is a vital aspect of livestock management. This research was conducted to
analyze correlation and regression models and to find the best regression formula to
predict body weight for female Batur sheep using body measurements. A total of 117
female Batur sheep aged 0.4 – 4 years in Batur Village, Banjarnegara, Central Java,
Indonesia, were collected. Body weight (BW) data were regressed and correlated to
body measurements (body length = BL, chest girth = CG, and withers height = WH) using
simple and multiple linear regression using the SPSS program. The results showed that
body measurements had a positive correlation with the BW, which CG had the highest
correlation (0.778), followed by BL (0.716) and WH (0.472). The best predictor for the
single predictor was CG. Meanwhile, for the multiple predictors, the fittest predictor was
a combination of BL and CG with the regression model BW = -45.777 + 0.761BL +
0.522CG with the highest r (0.830) and adjusted R2 (0.683); and the lowest RSE (12.159),
AIC (919,806), and BIC (922.506). This study concludes that CG and BL could be used as
a predictor for female Batur sheep's body weight.
Keywords: Batur sheep, linear regression, body weight, body size, phenotypic
characteristics
100
Performance of Broiler Chickens Supplemented with Porang
Glucomannan and Bacillus subtilis
Yenny Niken Larasati1, Eni Harmayani1, Jaka Widada1, and Nurliyani Nurliyani1
1Study Program of Biotechnology, Universitas Gadjah Mada, Depok, Sleman,

ABSTRACT
The present study was designed to investigate the effect of porang glucomannan and
Bacillus subtilis on performance of broiler chickens. Two hundred one-day-old broiler
chicks were kept for 35 days. The experiment was arranged as a completely randomized
design with 5 treatments and 4 replications. The treatments were T0 (control), T1
(prebiotic porang glucomannan), T2 (probiotic Bacillus subtilis), T3 (synbiotic porang
glucomannan and Bacillus subtilis), and T4 (synbiotic porang flour and Bacillus subtilis).
Parameters measured were body weight, feed intake, and feed conversion ratio. Overall
body weight in treatments T1, T2, T3, and T4 were greater (p<0.05) than T0. Moreover,
feed convertion ratio of T1, T2, T3, T4 were lower (p<0.05) than T0. However, there was
no effect of dietary treatments on feed intake. It was concluded that supplementation
of porang glucomannan and/ or Bacillus subtilis showed similar feed intake but body
weight and feed conversion ratio of broiler better than control.
Keywords: Bacillus subtilis, broiler chicken, performance, porang glucomannan
101
The Increase of Ruminant Population Based On The Potential of
Agricultural Waste as Animal Feed in Aceh Tamiang District
Yenni Yusriani1, Nora Usrina2 and Mustafa Sabri3
1 Aceh Assessment Institute for Agricultural Technology, Indonesia

2 Indonesian Agricultural Quarantine Development, Indonesia
3 Faculty of Veterinary Medicine, University Unsyiah, Indonesia
ABSTRACT
Livestock management includes the systems of maintainance, reproduction and waste

utilization. The purpose of writing was to examine the potential of feed sources for
ruminants (cows and buffaloes) and efforts to maintain continuity of feed availability
associated with ruminant livestock development strategies. The data used were primary
and secondary data of feed sources. The variables observed were consisted of beef
cattle and buffalo population, agricultural waste production, need for dry matter
digested (DDM) and carrying capacity. The results of the study showed that Seruway was
sub district with the highest population of cattle with 8,378 cows (4,933 ST) or 20.35 %
and Karang Baru was Sub District with the highest population of buffalo, namely 70
buffalo (34 ST) or 40.23 %. The high total production of agricultural waste was the
Manyak Payed sub-district, 5,029.32 DDM tonnes / year and the low district was Kuala
Simpang City because this sub-district is the capital of Aceh Tamiang Regency. The
agricultural commodity producing the most agricultural waste and was scattered in all
districts comes from rice with a total of 19,370.65 DDM tons / year. Although there were
not in all districts, cassava plants has the potential of 48,154.5 tonnes / year DDM. The
sub-district having a great opportunity was Karang Baru sub-district which produces
unutilized potential of 2,718.23 tons / year and has a carrying capacity of 2,384.41 (ST /
year). Based on this study, Kejuruan Muda sub-districts do not have potential because
it did not have sufficient agricultural waste for cattle and buffalo, it is possible that
breeders in this sub-district will get feed from plantation and other wastes. It can be
concluded that Aceh Tamiang District can increase the population of ruminants by
utilizing forage feed from agricultural waste.
Keywords: population increase, ruminants, feed
102
The Tenderness Quality of Sinjai Superior Kampong Chickens’ Meat by
Using Marinated of Pineapple Rind Powder at Different Marinated Time
Azmi Mangalisu1, Armita Permatasari2
1Animal Husbandry Study Program, Science and Technology Faculty, Universitas

Muhammadiyah Sinjai , Indonesia
2Aquatic Resources Management Study Program, Science and Technology Faculty,
Universitas Muhammadiyah Sinjai , Indonesia
ABSTRACT
Sinjai Superior Kampong Chickens (SSKC) was a type of kampong chicken that needed
special treatment to get the tender meat quality. The marinated process used
pineapple rind powder was expected to improve the quality of meat tenderness. The
purpose of this study was to carry out the marinated process by using pineapple rind
powder with different marinated time to find out the quality of meat tenderness such
as the value water holding capacity (WHC), shear force value (SFV) and cooking loss (CL).
This study was conducted experimentally by using a factorial completely randomized
design (CRD) with the stages that carried out marinated process by using pineapple rind
powder at different margination time. The tested variables in this study were the values
of WHC, SFV and CL. The result showed that the optimal WHC and SFV values on giving
the pineapple rind powder was as much as 20% for 90 minutes of marinated time, while
the optimal cooking loss 20% for 120 minutes of marinated time on Sinjai Superior
Kampong Chickens’ meat. These lead to the conclusion that the addition of pineapple
rind powder as much as 20% from weight of SSKC meat and marinated for 90 minutes
could improve the tenderness quality of SSKC meat.
Keywords: meat, marination, rind, time
103
The Effect of Antibiotic Growth Promoters (AGP) on Antibiotic Resistance
and the Digestive System of Broiler Chicken in Sleman, Yogyakarta
Tri Untari1 , Okti Herawati1 , Marla Anggita1 ,Widya Asmara1 , Agnesia Endang Tri
Hastuti Wahyuni1 , Michael Haryadi Wibowo1

Mada, Yogyakarta, Indonesia 55281
ABSTRACT
Chicken is a major source of animal protein consumption in Indonesia. The problem

facing the poultry industry is the incidence of resistance which increases mortality of the
chicken production. One of the causes of resistance case is the use of antibiotics in feed
additives. The public understanding about the effects of the use of antibiotic growth
promoters (AGP) in chickens in antibiotic resistance and the digestive tract of chicken
needs to be done to avoid the impact on economic losses and health problems. This
study aims to provide an understanding of the effects of the use of antibiotic growth
promoters (AGP) on antibiotic resistance and the digestive tract of broiler chickens. This
study was carried out at a broiler chicken farm in Sleman, Yogyakarta. Based on the
histopathological result of the digestive tract of chickens that were given antibiotics as
AGP, there was no inflammation occurs, but the administration of antibiotics caused
antibiotic resistance in various type of antibiotics including tetracycline (90% resistance),
streptomycin (60% resistance), amoxicillin (50% resistance), erythromycin (80%
resistance), and no resistance for gentamycin.
Keywords: chicken, antibiotic resistance, digestive tract
104
The Study of Pregnancy Rates (PR), Service per Conception (S/C), Calving
Rate (CR) and the Incidence of Dystocia in Peranakan Ongole (PO) Cows
That Inseminated by Various of Frozen Semen Breeds
Agung Budiyanto1 , Topas Wicaksono Priyo Jr1, Wisnu Krisna Nanditya2 and Rifia
Tiara Fani3
1Department of Reproduction and Obstetric, Faculty of Veterinary Medicine,

2Center of Animal Health, Department of Food and Agriculture,
Kulon Progo Regency, Indonesia

3Post Graduate of Veterinary Science, Faculty of Veterinary Medicine,
ABSTRACT
Artificial Insemination is one of the breeding techniques that has become popular in
Indonesia recently. Limousine and Simmental breeds of semen have been favorite
among farmers. It is worrying that the use of crossbreed breeding will reduce pregnancy
rates and increase the incidence of dystocia This study used 86 adult Peranakan Ongole
(PO) cows, 3 to 8 years age, good health and were maintained with a traditional
management system. Furthermore, PO cow was inseminated by PO, Simmental
Peranakan Ongole (SimPO) and Limousin Peranakan Ongole (LimPO) frozen semen then
Pregnancy Rate (PR) have checked by per rectal methods, then collected data of Calving
Interval (CI), Conception Rate (CR) and the incidence of dystocia in each group and then
analyzed by chi-square method. The results indicated that there was no difference
significantly in all parameters between treatment groups. PO cows have well adaptable
and have excellent reproductive anatomy and physiology so that they can support the
process of fertilization, pregnancy, and calving. Variation of semen breed that
inseminated to PO cow has no disorder effect on pregnancy and calving.
Keywords: calving, inseminated, PO cows, pregnancy, semen breed
105
Efficiency the Utilization of folow up palm plants foor increasing
Production Beef Cattle in District Pasaman Barat
Rahmi Wahyuni1, R.A. Dewi1,Harmaini1, S. Asmairicen1
1Balai
Pengkajian Teknologi Pertanian Sumatera Barat.
Jalan Raya Padang-Solok Km. 40 Sukarami 25361. Telp.(0755) 31122. Faks. (0755)
31138.
E-mail: bundanayla26@gmail.com
ABSTRACT
The Main Problem in the development of cattle business people in palm plantation, one
exeption availibility of Forage animal feed whether in quantity n quality. Other side the
existence of palm leaves the midrib has been waste of a disturbing and not been utilized
by farmers. To the touch technological innovations feed in te form of silage then palm
leaves the midrib has been animal feed worthy of in eating of cattle. The purpose of this
research is identify the effects the provisio of feen based silage palm leaves midrib
increased body weight on cattle beef and anilize efficiency the cost of animal feed.
Research is done in farmer group Tanjung Keramat in district Kinali, Pasaman Barat in
february until april 2017. Respondent are set down in purposive sampling. This research
used 17 tail local bulls created the average 2 until 2, 5 years. Treatment provides was
P1 control grass field is 10% from body weght (25 Kg) + bran is 10% from body weght
(2,5 Kg) , P2 granting of silage then palm leaves (10 kg) + bran (1,5 Kg) + gliserida ( 2
Kg). In an analysis using t table independent sample t-test α=5%. Result of research
shows P1 increase body weight cattle 0,68 kg/tail/day and P2 0,81 kg/ tail/ day .
Provision of feed based silage then palm leaves significant compared feed control t test
(-6,9) > t tabel (2,4). With efficiency cost feed during 90 days P1 (Rp. 967.500,-/ tail) and
P2 (Rp. 675.500,-/tail). So, with provision palm leaves besides increasing body weight
cattle, can also reducing the grass that drain time dan energy can reduce the cost daily
cattle feed.
Keywords: beef cattle, feed, palm leaves.
106
Placenta Expulsion-time on Different Age and Breed Cows
Erif Maha Nugraha Setyawan1 , Yosua Kristian Adi1, Topas Wicaksono Priyo1, Surya
Agus Prihatno1, Sri Gustari1, Asmarani Kusumawati1, Agung Budiyanto1
1Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2,
ABSTRACT
The third stage of birth is placenta expulsion which can occur about 30 minutes after the
release of the foetus but is more likely to occur between 180 to 300 minutes later. This
research is expected to provide an understanding about the different time on placenta
expulsion, the number of cotyledon and placenta weight that faced up by veterinarian.
Cows with a body condition score between 2.5 to 3.5 and ready to give birth from
various breeds were used in this study. The results showed that young cows (<5 years
old; 366,50 ± 30,97 minutes) significantly had a longer placenta expulsion time than old
cows (>5 years old; 251,29 ± 7,55 minutes; p<0,05). While the amount of cotyledon and
placenta weight did not differ between the two age groups. Based on breeds, the young
Ongole cows showed significant different on placenta expulsion time compared with old
ones (397,25 ± 61,60 vs 250,80 ± 14,79 minutes; p<0,05) but had no difference with the
young and old crossbreed cows. Young cows need more time for placenta expulsion than
the old ones and the young Ongole cows take the longest time compared to other age
and breed groups.
Keywords: Placenta expulsion, cows, age, breed
107
Antibiotic Resistance of Salmonella Sp. Isolated From Broilers in Poultry
Slaughterhouses in the Special Region of Yogyakarta
Santi Lestari 1,2, AETH Wahyuni 2, Heru Susetya 2, Hendra Wibawa 1
1Balai Besar Veteriner Wates Yogyakarta

2 Faculty of Veterinary Medicine, University of Gadjah Mada
ABSTRACT
The presence of Salmonella strains that were resistant to antibiotics in animal products
had important implications for public health. The purpose of this study was to identify
Salmonella sp. colected from broilers samples and to determine the resistance
characteristics of Salmonella sp. to various antibiotics. About caecum samples were
colected from poultry slaughterhouses in the Special Region of Yogyakarta Province.
The isolation and identification of Salmonella sp. using standard methods according to
SNI 2897: 2008. Testing for Antibiotic resistance using the dilution methode for liquid
automatically by using a sensititre device. This method measured the MIC (Minimum
Inhibitory Concentration). The results showed that 15 isolates of Salmonella sp. and 5
isolates of Salmonella enteritidis. Salmonella enteritidis isolates were resistant to
Antibiotics Nalidixic Acid (80%), Ciprofoxacin (20%) Tetracyclin (20%), and 100%
sensitive to Antibiotics Azythromycin, Cefotaxime, Ceftazidime, Chloramphenicol,
Gentamycin, Meropenem, and Trimetroprim. Meanwhile, Salmonella sp. were resistant
to Antibiotics Ampicillin (46.6%), Azithromycin (20%), Cefotaxime (26.6%),
Chloramphenicol (6.6%), Ciprofloxacin (80%), Gentamycin (33.3%), Nalidixic Acid (
93.3%), Tetracyclin (53.3%), and Trimetroprim (20%). All isolates of salmonella sp and
salmonella enteritidis were 100% sensitive to antibiotic meropenem
Keywords: Salmonella, Antibiotic resistance, Sensititre, Broilers.
108
Relationship Between Sheep Farmer’s Characteristics with the Animal
and Environmental Health Management Aspects in Batur Village,
Banjarnegara, Indonesia
Alek Ibrahim1 , Deny Setyo Wibowo1 , I Gede Suparta Budisatria2 , Rini Widayanti1 ,
and Wayan Tunas Artama1
1Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2,

ABSTRACT
The successful practice of animal husbandry is supported by good animal and

environmental health management aspects. This study examined the correlation
between the sheep farmer’s characteristics and the aspect of animal and environmental
health managements. The data collected by direct interview to 46 farmers in Batur
Village, Banjarnegara. Indonesia. The aspects of animal health management practice
(exercise, grooming, wool shearing, supplement feeding, and water drinking) and
environmental health management practice (sheepfold area clean-up, sanitation, and
waste processing) were divided into three categories, namely low, medium, and high
levels. The results shown that 60.9% of respondents were in the low level following by
medium level (37.0%) and high level (2.2%) of animal health management. Furthermore,
on the environmental health management showing 52.5% of respondents were in the
low level, 39.1% in the medium level, and 8.7% in the high level. There was a significant
relationship between farmer and farm characteristics (raising purpose, livestock
experience, and number of livestock) and animal health management practice. A
significant relationship was also shown between education, family labor, livestock
experience, and number of livestock by farmer toward environmental health
management practice. It may conclude that the low level of both health management
practices was dominant in Batur Village. Further extension and assistance were needed
to improve both management practices so that healthy livestock activities can be
realized, both in the animal and environmental aspects.
Keywords: correlation, livestock activities, management practice, smallholder farmers
109
Farmer and Farm Characteristics Associations with the Knowledge for
Sheep Estrus Detection in Batur Village, Banjarnegara, Indonesia
Alek Ibrahim1 , Wayan Tunas Artama1 , Rini Widayanti1 , Bayu Andri Atmoko2 , and I
Gede Suparta Budisatria2
1Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2,

ABSTRACT
The accuracy of the estrus detection was important in sheep breeding management.
This study aimed to determine the correlation between the farmer and farm
characteristics and the knowledge on estrus detection of their sheep. The data were
collected by interviewing 30 farmers, who have reised sheep with a breeding system for
at least three years experiences in Batur Village, Banjarnegara, Indonesia. The farmers’
knowledge for estrus detection was divided into three-level, namely low, medium, and
high. The results showed that the farmers’ knowledge for estrus detection was 53.3%,
23.3%, and 23.3% on the low, medium, and high levels, respectively. There was a
significant relationship between education, sheep farming experience, number of
sheep, the sheepfold position, stay duration in sheepfold, participation in extension, and
the ram ownership for mating toward knowledge of estrus detection level. It may
conclude that the low level of farmers’ knowledge for sheep estrus detection was
dominant in Batur Village. According to the close relationship between participation in
extension and the farmers’ knowledge for estrus detection, extension and assistance
were needed to increase the knowledge.
Keywords: relationship, good breeding practice, smallholder farmers, ewes
110
Stress Response of Bali Bulls Subjected To Shipping Transport
Yunita Wahyuningsih1, Puji Astuti2, Irkham Widiyono3
1Agriculture
Quarantine Centre Class I Pontianak, Kalimantan Barat 78113, Indonesia
2Department of Physiology, Faculty of Veterinary Medicine Universitas Gadjah Mada,
3Department of Internal Medicine, Faculty of Veterinary Medicine Universitas Gadjah
ABSTRACT
The study was aimed to evaluate the stress response of Bali bulls subjected to sea
transportation using a livestock carrier. Five clinically healthy adult Bali bulls were used
in this study. After loading, the animals were kept in a pen sized 2x8 m2 with straw
bedding and given straw and water ad libitum. After one day of rest on the ship, the
animals were transported for 108 hours from Kupang harbour Nusa Tenggara Timur to
Tanjungpriuk harbour Jakarta. During the trip, the animals were fed hay and provided
water ad libitum. Ship speed, wave height, temperatur, and humidity were recorded
during the trip. Venous blood samples were collected from jugular vein during the
resting period before the start of the trip and during the trip at hour 14, 38, and 108
(end of the trip). Serum was separated by centrifugation and stored at -20oC until
cortisol analysis. The concentration of cortisol was analyzed using ELISA. The results
showed that the average serum cortisol concentrations of Bali bulls before the start of
the trip, during the trip at hour 14, 38, and 108 was at the level 73.3±71.40 ng/mL,
139.13±120.38 ng/mL, 80.32±57.61 ng/mL, and 56.14±55.6 ng/mL, respectively. There
was no significant change of serum cortisol concentration before and during the
transportation (P>0.05). It is concluded that transportation using a livestock carrier
meets animal welfare standards and does not induce stress during the sea
transportation of Bali bulls.
Keywords: Bali bulls, cortisol, livestock carrier, sea transportation
111
Probiotics and Herbs Combination in Commercial Feed Additives as
Growth Promoter in Broiler Chicken
ThomasEmanuelManggotuNahak1, AgnesiaEndangTriHastutiWahyuni2, Yanuartono3,

CharlesRanggaTabbu4
¹Veterinary Science Study Program, Faculty of Veterinary Medicine, Universitas Gadjah

Mada, Jl. Fauna No. 2, Sleman, Yogyakarta
²Departement of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah
3Departement of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah

4Depertement of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Jl. Fauna No. 2, Sleman, Yogyakarta
ABSTRACT
The prohibition on the use of Antibiotic Growth Promoter (AGP) causes the use of
probiotics, herbs, enzymes and organic acids as an alternatives to AGP. This study aimed
to determine the role of combination of probiotic and herbs in commercial feed
additives on broiler performance. Sixty day-old male broiler were divided into 3 groups
where each group consisted of 20 chickens. Group I was given commercial feed, group
II was given commercial feed + AGP and group III was given commercial feed +
combination of probiotics, herbs, enzymes and organic acids. All chickens were kept for
5 weeks. Body weight gain (BWG), body weight (BW) and Feed Convertion Ratio (FCR)
were calculated every week. Carcass percentage was calculated at week 5. The results
obtained from BWG and BW on group III at weeks 4 and 5 weeks were higher and
significant difference (P˂0.05) compared to groups I and II. The percentage of carcasses
between groups did not have a significant difference (P˃0.05). FCR values in group III
from week 1 to weeks 5 were lower than groups I and II. The use of combination of
probiotics, herbs, enzymes and organic acids has proven to have a good role on broiler
performance so that it can replace AGP as a feed additive on broiler.
Keywords: Probiotics, Herbs, Enzymes, Organic Acids, Performance
112
Biodiversity Of Local Feeds Availability As A Nutritional Source To
Increase Bali Cattle Productivity In Bangli District, Bali
A. Sischa Jati Utami ¹* I Made Astagunawan¹* Yusti Pujiawati¹* and A.A.N.B.

Sarmuda Dinata¹*
1Assessment Institute for Agricultural Technology (AIAT) Bali

Jln. By Pass Ngurah Rai Pesanggran Denpasar, Tlp. (0361) 720498
*Corresponding author: siskajati@gmail.com
ABSTRACT
Indonesia is a tropical region that affects the availability of animal feed and waste by
products as feed ingredients for cattle. Abundant source of sunray make plant mature
fast and lowering nutrition value. Achieving self-sufficiency in beef can be done one of
them by increasing the nutritional value of feed, it is important to get a high nutritious
feed source to be developed. The availability of animal feed will affect livestock
productivity because high nutrition is needed to support livestock production. The use
of local food sources that have been proven to adapt to global climate change needs to
be done. This study aims to determine the type of local food in Bangli Regency which
has been tested to have a high nutritional content. The study was conducted by survey
method, sampling for proximate analysis at the Grati Pasuruan animal feed laboratory,
and field studies on the results of trials of various animal feeds. The technology
component that was added has provided sufficient results proving a daily weight gain.
The results are several types of local feed can be used as superior feed such as plants:
Indigofera sp, Dapdap, Gamal, Dadem (Fiscus fisculata), vines, and Indigofera sp. plants.
Which has a consecutive crude protein content: 27.74%; 26.54%; 21.45%; 15.65%. With
feed combination with this sorce of feed increased minimum 5% compare with kontrol.
in cconclusions with the use of highly nutritious feed, livestock productivity increase
then the number of livestock increase.
Keywords: nutrition, local feed source, livestock production
113
Effect of candidate feed additive from combination of Pinus merkusii
(jungh. & de vriese)) and Melaleuca leucadendra (l.) essential oil on
kinetic of gas production and emitted methane from ruminal
fermentation in vitro
Asih Kurniawati1*, Muhlisin1, Muhsin Al Anas1
1Faculty of Animal Science, Universitas Gadjah Mada, Jl Fauna No3 Bulaksumur

* Corresponding author: asihkurniawati@ugm.ac.id
ABSTRACT
The research was done to determine the effect candidate natural feed additive on
kinetic of gas production as representation of feed degradability and methane produced
during rumen fermentation. Three blends essential oil (BEO) as candidate of feed
additive were formulated from pine (Pinus merkusii (Jungh. and de Vriese)) and
eucalyptus (Melaleuca leucadendra (L.)) essential oil in ratio of 75:25, 50:50, and 25:75%
respectively for BEO1, BEO2 and BEO3. Every BEO was added into batch fermentation
system to get the doses in medium of 0, 100 and 200 µl/l. In vitro gas production
technique was conducted to simulate of rumen feed fermentation. From gas production
kinetic equation, all BEO addition did not affect the total potential gas produced and
also the potential gas produce form soluble as well as insoluble substrate. All
fermentation has the similar gas production rate. Furthermore, actual total gas
produced during fermentation did not affected by addition of BEO as well. Meanwhile
BEO1 at dose 200 µl/l and BEO 3 at doses 100 µl/l significantly reduced methane
production, in contrary BEO1 at level 100 µl/l increase methane production (P<0.05). It
could be concluded that BEO1 at dose 200 µl/l and BEO 3 at doses 100 µl/l had a potency
to be used as feed additive to reduced methane production without causing negative
effect on nutrient digestibility.
Keywords: blend essential oil, methane production, ruminal fermentation, gas kinetic
114
Water Holding Capacity of Muscles of Swamp Buffalo Raised with and
without a Proper Herd Health Program
Fhaisol, M.A.1, Rosly, S.3, Nasyatul, E.M.H.3, Lokman, I.1, Hasliza A.H.1, Zamri, M.S.2,
Zuki, A.B.1*
1Dept. of Veterinary Preclinical Science, 2Dept. of Veterinary Laboratory Services,

Faculty of Veterinary Medicine, Universiti Putra Malaysia
3Livestock Science Research Centre, Malaysian Agricultural Research and Development
Institute (MARDI)
*Corresponding author: zuki@upm.edu.my
ABSTRACT
Fresh meat contains approximately 70-75% of water. Water holding capacity is one of
the most important attributes of meat and influence storage quality, appearance and
eating experience and also contribute to profit-loss for meat entrepreneurs. The
factors that influence water holding capacity include animal species, sex, breed,
farming practices and post-slaughter storage and handling. Swamp buffalo meat
according to the previous reports are leaner than cattle, goat and sheep. Since
production system (farming practices) plays an important role in water holding
capacity, this study was conducted to determine and compare the water holding
capacity of muscles of swamp buffalo raised with and without a proper herd health
program at different ageing period. A total of 10 male swamp buffalo aged
approximately 24 months old were divided into two groups. Group one (n=4) was
raised with a proper herd health protocol (HHP), while group two (n=6) was raised
without a proper herd health protocol (NHHP). The drip loss, thawing loss and cooking
loss for longissimus dorsi (LD), supraspinatus (SS) and semitendinosus (ST) muscles
were evaluated at days 1, 7 and 14 ageing period. The results revealed that there was
no significant difference in drip loss between HHP and NHHP in all the muscles and at
different ageing period. However, there was significant difference (P≤0.05) for
thawing loss in LD, SS and ST muscles at different ageing period between HHP and
NHHP. The thawing loss was greater in LD and ST muscles at 14 days of ageing period
in both HHP and NHHP groups. The cooking loss for all the muscles was significantly
higher (P≤0.05) at days 7 ageing period in NHHP group while at day 14 ageing period
it was significantly higher in LD and ST muscles only as compared to HHP group. Thus,
this study showed that the muscles of swamp buffalo raised with a proper herd health
program evaluated against the physical force (freezing and heating) and ageing had a
better water holding capacity as compared to the swamp buffalo raised without a
proper herd health program.
Keyword: Swamp buffalo; Herd Health Program; Water holding capacity
115
Evaluation of Sperm DNA Fragmentation in Several Species by TUNEL
Assay as an Advanced Semen Quality Parameter
Kurniawan Dwi Prihantoko1, Makruf Arif1, Muhammad Farid1 , Asmarani

Kusumawati2*
1Postgraduate of Veterinary Science, Faculty of Veterinary Medicine, Universitas

Gadjah Mada, Jl. Fauna No.2, Karangmalang, Sleman, Yogyakarta 55281, Indonesia.
2Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, Jl. Fauna No. 2, Karangmalang, Sleman, Yogyakarta 55281,
Indonesia
ABSTRACT
Sperm DNA fragmentation has been shown to affect fertility rates as a result of artificial
reproductive technology. Research on the examination of sperm DNA fragmentation in
several animal species is still limited. This study aimed to adapt the terminal deoxynucleotidyl
transferasemediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation
examination in several animal sperm (bulls, roosters, and mice). The efficacy of using the
TUNEL method on sperm samples with different morphologies was the main focus of this
study. Semen samples were being collected from 4 samples of ongole grade bulls using the
artificial vagina method, 4 samples of KUB roosters by using the abdominal massage method,
and 4 samples of mice from the epididymal collection. Semen evaluation includes motility (%),
viability (%) using nigrosine eosin method, membrane integrity (%) using the hypo-osmotic
swelling test (HOST) method, and sperm DNA fragmentation (%) using the TUNEL method.
The results show that ongole grade bulls sperm DNA fragmentation was 2.25%. For the KUB
rooster sperm samples, the corresponding values was 1.25%. And then, for mice sperm
samples, the results show that mice sperm DNA fragmentation was 16.50%. Results in mice
showed the highest DNA fragmentation compared to other samples. Meanwhile, bulls sperm
samples showed the lowest DNA fragmentation. The significant differences in the mice sperm
samples could be affected by different chromatin qualities. The quality of chromatin in the
cell nucleus greatly determines the status of DNA-bound protamine which functions to
protect DNA. In addition, the sperm TUNEL method might be effective for assessing the sperm
DNA fragmentation of bull and mice sperm samples but not recommended for the rooster
sperm samples. However, the sperm DNA fragmentation can be used as an additional
parameter to provide a more comprehensive semen quality based on sperm DNA damage
Keywords: Sperm, DNA fragmentation, Semen quality, Semen evaluation, TUNEL
116
Most Probably Number (MPN) of Escherichia coli in milk from individual
and grouped dairy farms
Gilang Pandu1 and Yatri Drastini2*
1,2 Departement of Veterinary Public Health,

Faculty of Veterinary Medicine, University of Gadjah Mada,
Jl. Fauna 2, Karangmalang, Jogjakarta
+62 274 560862; Email: fkh@ugm.ac.id
*Corresponding author: drastini@ugm.ac.id
ABSTRACT
In previous study the quality of cow's milk production in the farms is not good enough.
This study aims to determine the Most Probably Number (MPN) of E coli in milk from
individual and grouped farms. A total of samples were four milk samples. Milk samples
were taken from three farms consisted of two individual and one grouped farms. Milk
samples taken directly after milking were put into sterilized plastic and carried using a
cooling box. Determination of E coli and counting of MPN were conducted by milk
samples were diluted in 10-1, 10-2, and 10-3 into 5 series of 9 ml Lauryl Sulphat Tryptose
Broth (LSTB) media in tubes containing Durham tubes. The media was incubated at 37
°C for 24 hours. Positive samples were inoculated into E coli (EC) broth and incubated at
44 °C for 24 hours. Positive samples from EC broth were inoculated into Buffered
Peptone Water (BPW) media and incubated at 44 °C for 48 hours. The samples in the
BPW were added with Kovacs reagent. The results showed all of tested samples
contained E. coli. The MPN value of milk from individual farm was 0.90 and that of
grouped farm was 0.99, but the milk was suitable for consumption because the MPN
value of E. coli was less than 3/mL.
Keywords: E. coli, milk, MPN.
117
Detection of Oxytetracycline Antibiotic Residue in Milk by Bioassay
Method
Yatri Drastini1, Dyah Ayu Widiasih1, Heru Susetya1, Widagdo Sri Nugroho1, Khrisdiana
Putri1, Roza Azizah Primatika1
1 Departement of Veteriner Public Health, Faculty of Veterinary Medicine

University of Gadjah Mada
Jl. Fauna 2 Karangmalang Yogyakarta
drastini@ugm.ac.id
ABSTRACT
Milk is one of the animal products being a very useful nutrition for people, while the
price of milk is not expensive. However, recently the presence of antibiotic residues in
fresh milk has been widely reported. Antibiotic usually used for treatment in dairy farm
is oxytetracycline. The aim of this research is to detect oxytetracycline antibiotic
residues in milk by bioassay method. The 14 samples of fresh milk were randomly taken
from dairy farms. The presence of oxytetracycline antibiotic residues was detected by
bioassay method using the standard bacterium Bacillus cereus 11778. It could be
concluded that three of 14 samples (21%) of fresh milk contained oxytetracycline
antibiotic residues indicated by an inhibition zone around the well filled by milk samples.
The bioassay test using 2.6 x 105 CFU / mL Bacillus cereus could detect 0.5 ug / mL of
oxytetracycline.
Keywords: antibiotic residue, bioassay, milk, oxytetracycline.
118
Chicken Meat Hygienic Standards Evaluation In Traditional Markets
Of Kulon Progo Regency, Indonesia
A. PURWANTORO 1,4,* , Y. DRASTINI 2, N. ELISSHA 3, N. EZEEKIEL 3,

VITHIYASHINI 3, W. T. ARTAMA 1,4
1. Department of Biochemistry, Faculty of Veterinary Medicine,

2. Department of Veterinary Public Health, Faculty of Veterinary Medicine,
3. Veterinary Science Study Programme, Faculty of Veterinary Medicine,
4. One Health Collaborating Center,
Universitas Gadjah Mada, Yogyakarta, Indonesia.
Corresponding author: arispurwantoro@ugm.ac.id
ABSTRACT
Chicken meat is a primary source of animal protein for Indonesian households. The meat sells in
traditional markets that have minimum infrastructures and facilities, besides it sells at
convenient stores (mini, midi, super, and hypermarkets) which have ideal conditions. This study
evaluates the hygienic standards of chicken meat trading in the traditional market of Kulon
Progo Regency. The meat sellers who participated in the study were selected randomly from the
markets above using multistage sampling. The investigators visited the markets and interviewed
them based on a structured questionnaire. Evaluation of hygienic standards of the chicken meat
kiosks including criteria of the hood, table, gutter, and seller protective equipment. Data were
coded and stored in a spreadsheet. Most information obtained was qualitative data and is
presented as summarized opinions. The results can be used as recommendation data for all
stakeholders (chicken farmers, meat sellers, veterinarians, and other government authorities).
The authorities should campaign public health awareness to fulfill the ideal hygienic standards
of chicken meat trading.
Keywords: hygienic standard, chicken meat trading, traditional market, Kulon Progo, Indonesia.
119
120
Virus Isolation from Culex tritaeniorhynchus, the Primary Vector of
Japanese Encephalitis, Using NIID-CTR Cell Lines
Astri Nur Faizah1, Daisuke Kobayashi2,3, Michael Amoa-Bosompem4, Haruhiko Isawa2,

Kyeong Soon Kim5, Mamoru Watanabe2, Kozue Miura1, Kazuhiro Hirayama1, Kyoko
Sawabe2
1 Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life

Sciences, The University of Tokyo
2 Department of Medical Entomology, National Institute of Infectious Diseases
3 Japan Agency for Medical Research and Development
4 Department of Environmental Parasitology, Tokyo Medical and Dental University
5 Joint Department of Veterinary Medicine, Tottori University
ABSTRACT
Mosquito virome, based on host range, is divided into arboviruses (which infect
vertebrates) and insect-specific viruses (ISV). Japanese encephalitis virus (JEV), an
arbovirus that cause viral encephalitis in most Asian countries, is mainly transmitted to
humans by the Culex tritaeniorhynchus mosquito. Efforts have been put into virome
determination of the mosquito in order to better understand the transmission cycle of
JEV and elucidate the potential role of insect-specific viruses (ISVs), which naturally
infect mosquitoes, as biocontrol. Recent studies have indicated the ability of ISVs to
affect a vector’s competency in transmitting arbovirus, hence the isolation of ISV is vital
in the preliminary steps of viral interference study. Aedes albopictus’ C6/36 is the
standard mosquito cell line for studying arboviruses. While highly permissive to
numerous viruses, C6/36 may fail to propagate some insect viruses, hindering efforts to
understand the role of ISVs. Thus, here we aimed to expand the virus isolation method
to obtain more insect viruses that could not be isolated by C6/36, by using a different
mosquito cell line: NIID-CTR derived from Cx. tritaeniorhynchus mosquito. JE primary
vector mosquitoes, Cx. tritaeniorhynchus, were collected in July and September 2017
from Ishikawa and Tottori prefecture, Japan. Four pools, each consisted of 25 female
mosquitoes, were crushed, filtered, and homogenized before the inoculation onto a
monolayer of NIID-CTR cell line and incubated at 25 °C for 7 days. The isolate was sub-
cultured twice in blind passages to propagate viruses. Final supernatants were
subsequently enzyme-treated and RNA extracted for Next-generation sequencer (NGS).
One pool was observed to cause cytopathic effect (CPE) and NGS confirmed the presence
of Culex tritaeniorhynchus anphevirus (CtAV), a negative single-stranded RNA virus
belonged to genus anphevirus family Xinmoviridae. A time course assay verified the
successful propagation of the virus overtime in NIID-CTR cell line, on the other hand, the
121
virus was reportedly unable to propagate in C6/36 cells. This study has not only exhibited
a new virus isolation method from a JE vector, but also demonstrates the relatively
higher efficiency in combining the two mosquito cell lines in studying virus-host
interaction. The combination may also accurately model ISV-arbovirus interactions in the
host environment to a larger extent, unlocking feasibilities for future studies.
Keyword: Japanese encephalitis virus, Culex tritaeniorhynchus, virus isolation, cell
culture, NIID-CTR, NGS
122
Detection of Babesiosis in Cattle and Buffalo Using duplex PCR and Thin
Blood smear
Dyah Haryuningtyas Sawitri1, A.H. Wardhana1, F.Ekawasti1, D.A.Dewi2
1Indonesian Research Centre for Veterinary Science
Jl. RE. Martadinata no 30 Bogor 16114
2Sekolah Tinggi Penyuluh Pertanian, Magelang-Indonesia
Corresponding email : dyah.haryuningtyas@gmail.com
ABSTRACT
Bovine babesiosis is a tick-borne disease caused by the intra-erythrocytic protozoan
parasite Babesia. Two species which commonly, Babesia bovis and Babesia bigemina,
are economically important in tropical and subtropical regions of the world including
Indonesia. Diagnosis of Babesia sp is commonly determined with a conventional
parasitological technique, thin blood smear with Giemza staining. However, this
technique has limitations in subclinical infections. Polymerase chain reaction (PCR)
shows high sensitivity, which is able to identify parasites at a much lower amount. The
aim of this study was to detect B. bovis and B. bigemina in blood cattle and buffaloes
from fields a conventional parasitological method, the thin blood smear. Moreover, the
study also compared effectiveness of a single and duplex PCRs in order to detect the
parasites. . One hundred and ninety eight blood samples from cattle and buffalo were
collected in Kunak (West Java), Pemalang, and Brebes (Central Java). The existence of
the parasite was assed using the thin blood smear. In addition, the PCR assay was
employed using primers Bovar 2A and Bb1/Bb2 specific for B. bovis and B. bigemina,
respectively. The results revealed that 14 out of 198 blood samples (7,1%) were positive
of Babesia sp based on the parasitological observation. The result of a single PCR
demonstrated that 72 samples (36%) were positive B. bovis, meanwhile 104 samples
(52.5%) were successfully identified B. bovis and B. bigemina by duplex PCR. This result
indicated that duplex PCR has faster and more economic for surveying the babesiosis in
epidemiological studies compared to the single PCR.
Keywords: Babesia bovis, Babesia bigemina, PCR single, PCR duplex, Thin blood smear
123
Polymorphism of CSN1S1 (g.12164G>A) and CSN2 (g.8913 C>A) Genes in
Pure and Cross Dairy Goats
Anneke Anggraeni1, Andi Baso Lompengeng Ishak1, Lailatus Syifa2, Oktiq Kurnia Sari2,
and Cece Sumantri2
1Indonesian Research Institute for Animal Production (IRIAP), PO Box 221 Ciawi, Bogor,
Indonesia
2Faculty of Animal Science, Bogor Agriculture University, Bogor, Indonesia
ABSTRACT
Genetic polymorphism of Casein genes can be considered as molecular markers for

selection of milk protein contents. Alpha-S1 Casein (CSN1S1) and β-Casein (CSN2) genes
directly influence on milk protein fractions. Genetic polymorphisms of CSN1S1
(g.12164G>A) and CSN2 (g.8913C>A) genes were identified by PCR-RFLP technique using
Dde1 restriction enzyme. Animal samples were pure dairy goats providing PE (5 hds.)
and Saanen (8 hds.), as well as their crosses providing Sapera (50% Saanen, 50% PE) (51
hds.) and SaanPE (75% Saanen, 25% PE) (3 hds.) at IRIAP dairy goat station. Allele
frequency, genotype frequency, heterozygosity value, and Hardy-Weinberg (H-W)
equilibrium value were analyzed by Popgen32 program. The CSN1S1_g.12164G>A locus
resulted in two alleles, i.e. G allele (192 bp, 145 bp, and 101 bp) and A allele (337 bp and
101 bp). Dairy goats of possessing the G allele from the highest frequenciest were
successively Saanen (0.625), Sapera (0.578), PE (0.400), and SaanPE (0.333). Most dairy
goats were heterozygote (Ho>He) and in H-W equilibrium (q2count < q2 P0.05). While
CSN2_g.8913C>A locus was monomorphic for presenting only C allele (233 bp and 162
bp), without A allele (416 bp). The existence of genotype variation of the CSN1S1 gene
at g.12164G>A SNP could be a potencial molecular marker of selection on milk protein
in dairy goat.
Keywords: dairy goats, CSN1S1 gene, CSN2 gene, genetic polymorphism
124
Exploration of Growth Family (GH, GHRH and PIT-1) Genes
Polymorphisms of Local Swamp Buffalo for Productivity Improvement in
North Tapanuli Regency, North Sumatra
Anneke Anggraeni1), Chalid Thalib1), and Angga Ardhati Rani Hapsari1)
1) Indonesian Research Institute for Animal Production, PO Box 221, Ciawi, Bogor.
ABSTRACT
Genetic improvement of livestock productivity can be done through molecular selection

on the genes controlling growth traits. Genetic polymorphism of the growth gene
families, providing GH, GHRH, and PIT1 genes, were studied in local swamp buffalo (106
hds.) from a government buffalo breeding station and smallholders in North Tapanuli
District, North Sumatra Province. Genotype variants of the three genes were identified
by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)
method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT-
1 gene). Data were analyzed for allele frequency, genotype frequency, Hardy-Weinberg
equilibrium, heterozygosity values, and PIC values. Genotyping results of the three loci
of the observed swamp buffalo showed monomorphic. Locus GH_g.1547C>G,
GHRH_g.4666G>C, and PIT-1_g.1257A>T produced only one type genotype, respectively
GG, CC, and TT, with one type of allele, respectively G, C, and T. Observation
heterozygosity values (Ho) and expectation (He) and the PIC value for each locus was
0.00. It could be suggested to increase the genotype frequency of the three growth
genes that are positively associated with the growth traits of the swamp buffalo
observed.
Keywords: growth gene family, PCR-RFLP, swamp buffalo
125
The Antimicrobial Activity of leaf and fruit crude extract of Calabash
(Crescentia cujete L)
Patricius Kianto Atmodjo, Boy Rahardja Sidharta
Fakultas Teknobiologi Universitas Atma Jaya Yogyakarta

Jl. Babarsari 44 Yogyakarta, Indonesia
ABSTRACT
Antibiotic resistance is a big problem in medication, food security, and human
development goals. Indonesia has a very huge diversity in term of medicinal plants,
which are proven to cure many illnesses by their ancestors, including Calabash.
However, research on the bioactive substances from the leaves and fruit of calabash as
antimicrobial are dearth. The objectives of the research are: a) to determine bioactive
substances found in the leaves and fruit of Calabash which have antimicrobial activities,
b) to reveal the antimicrobe activity of leaves and fruit extract of calabash to two(2) )
pathogenic bacteria and two (2) pathogenic fungus, c) to determine minimum inhibitory
concentration (MIC) of calabash’s leavesand fruit extract to four (4) pathogenic
microbes.The methods applied in this research are as follows. The bioactive substances
were extracted using maceration method with ethanol as the solvent. The extracts were
then analyzed using GC-MS equipment and tested to four (4) pathogenic microbes in
agar diffusion method to determine the inhibition zones of the extracts. Later, the
extract were also used to determine the MIC of the extracts by dilution technique.This
present research found that fruit and leaves extract have many bioactive substance
such as saponin, flavonoid, terpenoid and alcaloid that have low-medium antibacterial
and antifungus activity, with minimum concentration of inhibition 50% for bacteria and
75% for fungus., which later can be developed into products or antibacterial and
antifungus drugs that may give benefits to Indonesian people and the world
Keywords: Calabash (Crescentia cujete Lf.), antibacterial, antifungus, etanol extract,
phytochemistryt
126
Potential of Polylactic-Co-Glycolic Acid (PLGA) for Delivery DNA Vaccine
Model pEGFP-C1
Ziana Warsani1, Lalu Unsunnidhal2,3, Indra Lesmana Rahayu1, and Asmarani

Kusumawati1,2*
1Research
Center of Biotechnology, University Gadjah Mada,
Yogyakarta, 55281, Indonesia
University Gadjah Mada, Yogyakarta, 55281, Indonesia

3Biomedical Field, Nursing Study Program, STIKES Yarsi Mataram,
West Nusa Tenggara, 83361, Indonesia
ABSTRACT
The development of a vaccine for Jembrana Disease is needed to prevent losses to the
Bali cattle industry in Indonesia. A DNA vaccine candidate will be developed that
requires a carrier system to work. Polylactic-Co-Glycolic Acid (PLGA) has potential as a
carrier system for recombinant DNA. This study aims to evaluate the in vitro potential
of PLGA as a carrier system for DNA recombinant (PEGFP-C1) as a model carrier system
for the DNA vaccine. The research method used was the transformation to obtain
transformant-pEGFP-C1 bacteria, isolation of pEGFP-C1, confirmation of pEGFP-C1,
preparation of PLGA nanoparticles solution with pEGFP-C1, stability test with Dnase I
and Nanoparticle Cytotoxicity Test. The results obtained from this study were the
successful acquisition of transformant bacteria, confirmed linear with a size of 4711 bp
based on restriction enzyme tests, the PLGA: DNA: PVA ratio for formulations with
optimal entrapment was 4%: 0.5%: 2%, the PLGA complex with pEGFP-C1 succeeded in
protecting pEGFP-C1 from enzymatic degradation and the results of the percentage of
viability of the cytotoxic test on PLGA-pEGFP-C1 were above 90%, so it can be concluded
that PLGA has good potential as a carrier agent for eukaryote expression plasmids.
Keywords: Jembrana Disease, DNA Vaccine, Carrier Agent, PLGA
127
Microorganism on Leather Defects: producing protease industry in the
future
Novita Kurniawati1; Eka Luciana2; Nanung Agus Fitriyanto1, Mohammad Zainal

Abidin1, Satyaguna Rakhmatulloh1; Yuny Erwanto1; Zaenal Bachruddin1; Rusman1;
Lies Myra Yusiati1; Ambar Pertiwiningrum1*
1 Univeritas Gadjah Mada, Indonesia
2 Balai Besar Kulit, Karet, dan Plastik Yogyakarta, Indonesia
* Corresponding author: artiwi@mail.ugm.ac.id
ABSTRACT
Leather defect sources collected by our college Balai Besar Karet, Kulit, dan Plastik, D.I
Yogyakarta (BBKKP YK), Indonesia. They give three type of leather defect base on
processing as pickle, wet blue, and (leather artickle) upper shoes. Microorganism leather
defect were grown at minimal mineral medium and lowest (1/200 v/v) nutrient medium
and added two precent gelatine bovine Sigma-Aldrich. 1cm2 each leather defect slices
into smallest size to make easiest microbial leather defect soluble into liquid medium
during 7 days on open air rotary incubation and ambient temperature were used at 28°C
until 30°C. Here, we show ability the protease microbial leather defect and isolation
method on it. Protease should be the most causing leather damage than the other
enzyme for making the defect. The second of isolation method after cotton stick as
scratch ose on agar plates is centrifugation liquid medium at 15.1G, 20minutes
separation without any temperature control. We get four microbials isolation are yellow
colony, yellow-white colony, white colony white slime colony, and red colony. The
microbial colony forming at 38°C and the positive protease from 7 more days growth
liquid medium shown produce gases at pickle leather defects using test tube glass
diameter 0.8cm, 15cm length.
Keywords: BBKKP YK, pickle, wet blue, leather article
128
Identification of IGF1 and MSTN genes of Lakor Goat from Lakor island,
Southwest Maluku Regency encoded growth trait
Maman Rumanta1, Rony Marsyal Kunda2 , Slamet Diah Volkandari3, and Pieter
Kakisina2
1Department of Biology Education, Study Program, Faculty of Education and Teacher

Training, Universitas Terbuka, Jl. Cabe Raya, Pondok Cabe, Pamulang, Tangerang
Selatan, Jakarta, Indonesia,
2Department of Biology, Faculty of Mathematics and Natural Science, Universitas
Pattimura, Jl. Ir. M. Putuhena Poka, Ambon City, Maluku, Ambon, Indonesia.
3Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya
Bogor km 46, Cibinong, Bogor, West Java
ABSTRACT
Lakor goat is one of local goat in Indonesia and endemic from Lakor island (coral island)
at Southwest Maluku. Those goat has a good adaptation in less forage and high
temperature. Lakor goat is source of protein in that island. Insulin Growth Factor 1 (IGF1)
and caprine Myostatin (MSTN) are candidate gene for growth traits and muscle growth.
This study aimed to identify of IGF1 and MSTN gene of Lakor goat from Lakor island at
Southwest Maluku Region. Sixty three animals were used in this study. Hair follicles were
collected from tail as a DNA source and extracted using DNA isolation kit. Genotyping
those genes used PCR-RFLP method, HaeIII restriction enzyme for IGF1 gene and DraI
for MSTN gene. Result showed that all samples have monomorphic type in both of IGF1
(BB genotype) and MSTN (AA genotype) genes. Isolated area is suspected causing low of
genetic diversity in lakor goat population.
Keywords: IGF1 gene, MSTN gene, genetic diversity, Lakor goat, Lakor island Maluku
129
Antagonistic Activity of Marine Bacteria from Karimun Island, Indonesia
Ruby Setiawan1 , and Ade Lia Putri1
1Research Center for Biology, Indonesian Institute of Sciences,

Cibinong, 16911, Indonesia
ABSTRACT
Antimicrobial resistance (AMR) is becoming a major global crisis to the public healthcare
system. One of the issues is the misuse of antimicrobials that will accelerate the AMR
phenomenon. Discovering new antimicrobial compounds from various sources and
places is an alternative way to resolve this problem. Marine bacteria were known as a
new promising source for bioactive compounds. A total of 65 bacterial isolates have
been successfully isolated from the seawater and marine sediment from Karimun Island.
All of the bacterial isolates were subjected to antagonistic activity against Bacillus
subtilis, Escherichia coli, and Staphylococcus aureus. Out of the 65 isolates, 12 isolates
showed activity against test bacteria; 7 isolates against B.subtilis, 2 isolates against
S.aureus, 1 isolate against E.coli, 1 isolate against B.subtilis and S.aureus, and 1 isolate
against S.aureus and E.coli. The 16S rDNA sequences showed the selected isolates
belong to Kocuria polaris, Micrococcus aloeverae, Cellulosimicrobium funkei,
Nocardioides cavernae, Gordonia bronchialis, Micromonospora aurantiaca, Mumia
xiangluensis, and Pseudoalteromonas shioyasakiensis.
Keywords: antimicrobial resistance, marine bacteria, Karimun island, antagonistic

activity.
130
Molecular study of bone morphogenetic protein-15 (BMP-15) gene in
Sumba Ongole cattle, Sumba island
Cynthia Dewi Gaina, , Maxs U.E. Sanam1, WMN Nalley2 and Imanuel Benu2
1Faculty of Veterinary Medicine, University of Nusa Cendana, Kupang, Indonesia
2 Faculty of Animal Science, University of Nusa Cendana, Kupang, Indonesia
ABSTRACT
The aim of this research was to study BMP 15 gene in Sumba Ongole cattle (Bos indicus)
which extensively reared in Sumba island. This gene has been known for its function in
regulating reproductive performance in female cows. The blood sample was taken from
48 SO cattle. The first step was DNA isolation from blood, the second step was
electrophoresis and extraction then it continued with optimization of annealing
temperature, amplification and sequencing. The results of sequencing analyzed using
software program named BasicLocal Alignment Search Tool (BLAST). The annealing
temperature optimization results obtained temperature of 58 ° C was the proper
temperature to do amplification. All samples studied with BMP15 gene amplification
gained results of 350 bp. Results of BLAST program analysis and Mega 6.01 program
shows a close genetic relationship, characterized by the result sequence of BMP 15 of
Sumba Ongole possessing proximity to other ruminants, such as the Bos Taurus and
Capra hircus breed. The results of this study can be concluded that the BMP 15 is
amplified by 350 bp, and has the adjacency of nucleotide sequence with the BMP 15
gene in some other types of ruminants.
Keywords: BMP 15, genes, Ongole, Sumba
131
Effect of Biofertilizer and Salinity Stress on Amaranthus tricolor L.
Growth and Total Leaf Chlorophyll Content
Nadhifa Athaya Khairunnisa1 , Dwi Umi Siswanti2
1Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Sel., Special Region of
Yogyakarta 55281
2Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Sel., Special Region of
Yogyakarta 55281
ABSTRACT
Amaranth is one of the most consumed vegetable in Indonesia. Plant’s production can
be affected by productivity and environmental factors, called abiotic stresses. Salinity is
an abiotic stressing factor that can affects plant growth, becoming an issue in the
agriculture field. Biofertilizer is a fertilizer that contains microbial elements needed by
plant growth. Microbes play an important roles for the nutrient cycle in soil which can
increase the growth of plant. This research aimed to analyze the effect of biofertilizer
combined with NaCl on Amaranthus tricolor L. growth. The biofertilizer dose used in this
study was 10 L/ha, 20 L /ha and 30 L /ha, each combined with manure fertilizer and 2500
ppm, 5000 ppm, 7500 ppm, 10.000 ppm of NaCl. The parameters measured were plant
height, number of leaves, and chlorophyll content. The results revealed that the highest
amaranth growth was seen in 0 l/ ha of biofertilizer combined with 7500 ppm NaCl
treatment, number of leaves in 10 l/ha biofertilizer and 2500 ppm NaCl, and chlorophyll
content in 30 l/ha biofertilizer. It can be concluded that biofertilizer didn’t affect plant
growth but fertilizer does, and the most appropriate biofertilizer dose for chlorophyll
content was 30 l/ha.
Keywords: Amaranthus, Biofertilizer, Chlorophyll Content, Productivity, Saline

Condition.
132
EXPLORATION OF SECONDARY METABOLITE COMPOUNDS OF MELON
FRUIT (Cucumis Melo L. 'GMP') EXTRACT USING LIQUID
CHROMATOGRAPH MASS SPECTROMETRY (LCMS) METHOD
Muhammad Zulfikar1 , Rohmi Salamah2 , Wiko Arif Wibowo3 Budi Setiadi Daryono4 ,
Slamet Widiyanto1
1,2Laboratory of animal physiology, Faculty of Biology, Universitas Gadjah Mada, Jalan

Teknika Selatan, Sekip Utara, 55281 Yogyakarta, Indonesia
3,4Laboratory of Genetic and Breeding, Faculty of Biology, Universitas Gadjah Mada,
Jalan Teknika Selatan, Sekip Utara, 55281 Yogyakarta, Indonesia

Corresponding Author: slametbio@ugm.ac.id
ABSTRACT
Melon (Cucumis melo L.) belongs to the Cucurbitaceae family. Melon cv. Gama Melon
Parfum (GMP), a new cultivar of melon, which is the result of a cross between ♀NO3
and ♂MR5 melons. This research was conducted to analyze the content of secondary
metabolites in melon (Cucumis melo L.) cv. Gama Melon Parfum (GMP) using the Liquid
Chromatograph Mass Spectrometry (LCMS) method. The results of this analysis showed
that there were 57 secondary metabolite compounds. The secondary metabolite
compound with the largest composition is chlorogenic acid, which is 7,21177%. There
are 11 groups for 57 secondary metabolite compounds present in the extract of melon
cv Gama Melon Parfum (GMP) rind. The group with the largest composition is Flavonoid
compounds with 34.05% and Phenolic compounds with 32.68%. The secondary
metabolites contained have important phytochemical activities, including antidiabetic,
antibacterial, antioxidant, antihepatotoxic, anti-inflammatory, anticancer, and so on.
Keywords: GMP, secondary metabolites, LCMS
133
Full-length 16S rRNA Gene Sequencing to Detect Zoonotic Pathogens in
Reptiles Using Oxford Nanopore MinION
Sugiyono Saputra1Anik B. Dharmayanthi2, Anggia Prasetyoputri3, Syam B. Iryanto4,

Andri Wardiana3, Isa Nuryana3, Ade Andriani3, Ahmad Fathoni3, Ratih A. Ningrum3,
Mumpuni Mumpuni2, Evy Arida2 and Amir Hamidy2
1Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Sciences
3Research Center for Biotechnology, Indonesian Institute of Sciences
4Research Center for Informatics, Indonesian Institute of Sciences
ABSTRACT
Zoonotic pathogens from wild reptiles are often underestimated but it has become a
growing concern due to massive hunting for consumption as bushmeat and medicine.
This study aimed to detect gut microbiota that may be potential as zoonotic pathogens
in two species of reptiles, a Javanese Keelback Water snake (Fowlea melanzostus) and a
Tokay gecko (Gekko gecko) using metagenomic approach. A pair of primer 1492 R and
27 F was used to amplify approximately 1500 bp of 16S rRNA gene before subjected to
library preparation and 16h-run in Oxford Nanopore MinION sequencer. In total,
1.919.359 reads were generated and successfully classified (96%) and resulting 1438 and
1900 operational taxonomic unit (OTU) for Javanese Keelback Water snake and Tokay
gecko, respectively. In Javanese Keelback Water snake sample, Plesiomonas shigelloides,
Bacteroides eggerthii and Staphylococcus cohnii were among the dominant species
while in Tokay gecko was dominated by Clostridium bolteae, Akkermansia municiphila
and C. saccharolyticum. Several notable zoonotic pathogens such us Salmonella, Listeria,
Shigella, Vibrio were detected and common in both samples. Yersinia spp. was observed
in snake sample only while Leptrospira spp. only observed in gecko sample. We
demonstrate that full length 16S sequencing offer comprehensive detection of multiple
potential pathogens as complementary to detection by conventional PCR and culture.
Keywords: Zoonotic pathogens, reptiles, 16S rRNA, Oxford Nanopore MinION.
134
The Development and Optimization of Primer Sets Used to Study The
Relative Expression of Androgen Receptor Gene in Turkey (Melleagris
gallopavo)
Herjuno Ari Nugroho1*, Rini Widayanti2, Tri Wahyu Pangestiningsih3, Eli Supriyani2
1ResearchCenter for Biology, Indonesian Institute of Sciences (LIPI), Jl. Jakarta-Bogor

km 46, Cibinong, West Java, Indonesia 16911.
Mada, Jl. Fauna 02, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia 55281
3Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl.
Fauna 02, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia 55281

*corresponding author: herj001@lipi.go.id
ABSTRACT
The expression of Androgen Receptor (AR) Gene is essential in testes maturation, as

shown in Turkey. The expression of the gene during puberty and testes maturation can
be used as reference for Turkey’s breeding program. The aims of this research were to
develop and optimize two sets of primers that can be used for relative expression study
using qPCR technology. The primers were designed to amplify specific region in AR Gene
as the main target of the study, and β-actin Gene as internal control. The primers were
tested using in-silico and amplicon sequencing, and efficiency using constructed
standard curve from serially diluted reactions. According to in-silico and sequencing, the
primers amplified the corresponding region of the respective targets. The primer for AR
Gene showed efficiency 98,03%, slope -3,37 and R2 0,995, meanwhile for β-actin Gene
resulted in efficiency 98,01%, slope -3,371 and R2 0,999. The efficiencies for both primers
were passed the standard (93-103%) for relative expression study. Melting curve
analysis showed that there no any non-specific amplifications were found for both
primers. According to the several tests, we suggest that the primers were acceptable to
be used for relative expression study of AR gene in Turkey.
Keywords: Androgen Receptor Gene, Primer, optimization, Turkey
135
OPTIMIZATION OF DIFFERENT PRIMERS IN THE DETECTION OF
LACTOFERRIN GENE FROM DIFFERENT BREEDS OF GOATS THROUGH
POLYMERASE CHAIN REACTION
Guillermo Allen Paul
Tarlac Agricultural University

*Corresponding author: drallenguillermo@tau.edu.ph
ABSTRACT
The study was conducted to detect lactoferrin gene in different breeds of dairy goats
and to determine the breed which lactoferrin gene is best expressed. The breeds of
dairy goats used in this study were Saanen, Anglo-Nubian, and Upgraded goats.
Lactoferrin gene was amplified using polymerase chain reaction (PCR) and was detected
using gel electrophoresis. The data gathered were statistically analyzed using the
Analysis of Variance (ANOVA). Each sample from different breeds was compared by
means and percentage. The result revealed that lactoferrin gene is best expressed in
Saanen which had the highest number of positive goats with 80% out of its total samples.
In the primers used, primer 2 was the best primer in the detection of lactoferrin gene in
which eight (8) out of the nine (9) samples was positive in this primer. Based on the
result of the study, Saanen has the greatest concentration of lactoferrin gene and is
found to be the most disease-resistant breed of goat for dairy production considering
that lactoferrin in goat has an antibacterial, antifungal, antiviral and anticarcinogenic
property. However, on the analysis of variance, it was found out that there was no
significant difference between the three breeds of goats based on the expression of
lactoferrin gene.
Keywords. Lactoferrin Gene, Dairy Goats, Breed, Polymerase Chain Reaction, Gel
Electrophoresis
136
Recombinant Protein of SAG1 and Native Protein for Toxoplasmosis
Detection
Didik T Subekti1, Tri Wijayanti2, Dewi Marbawati2, Zumrotus Sholichah2, Sulinawati

Fong3
1 Indonesian Research Center for Veterinary Science, Bogor

2 Center of Health Research and Development, Banjarnegara
3 Disease Investigation Center, Bandar Lampung
ABSTRACT
The validation and compatibility test between ELISA toxoplasmosis using native protein
and recombinant protein have been carried out. Native protein is a protein from the
takizoit of Toxoplasma gondii antigen solubles. The recombinant protein used is the
tachyzoite surface protein known as SAG1 (surface antigen 1). The results of the
evaluation of 195 samples of human sera that were found to be true positive and true
negative indicated that there were differences between the two types of antigens. The
use of recombinant antigen protein resulted in performance in the form of sensitivity
and specificity of 84.73% and 90.79%, respectively, in the ROC analysis. In the 2x2 table
analysis, the performance in the form of sensitivity and specificity was 85.37% and
90.28%, respectively. The use of native protein as antigen showed better sensitivity and
specificity, namely 92.7% and 97.2% in the analysis with ROC and 2x2 table. In general,
the diagnostic accuracy of Toxoplasmosis ELISA using the recombinant protein antigen
SAG1 was 87.18% while using the native protein antigen from T.gondii tachyzoite was
94.36%.
Keywords: protein, T. gondii, SAG1, detection, takizoit
137
Restriction fragment length polymorphism (RFLP) analysis genes of
Toxoplasma gondii using enzym DdeI
Ekawasti Fitrine1.* , Cahyaningsih Umi2 , Sadi’ah Siti2 , Dharmayanti NLPI1 and Subekti
Didik1
1Indonesia
Research Center for Veterinary Science
2IPB University, Bogor
*Corresponding author: fitrineekawasti@gmail.com
ABSTRACT
Toxoplasma gondii is an unicellular coccidian parasite with worldwide distribution and

it is an important zoonosis. T. gondii is a widespread parasite able to infect virtually any
nucleated cells of warm-blooded hosts. The pathogenicity of T. gondii depends on the
type which originated from their clonal population. Each type has different implication
on clinical immunopathogenesis. More accurate T. gondii type determination
techniques have recently been developed using genetic markers. Diagnosis of
toxoplasmosis has been improved by the emergence of molecular technologies to
amplify parasite’s nucleic acids. PCR-RFLP (polymerase chain reaction-restriction
fragment length polymorphism) was used to detect polymorphism of DNA using
restriction enzyme. Here, we used ten PCR-RFLP markers in one restriction-enzyme DdeI
reaction by agarose gel electrophoresis. This study provides a set of PCR-RFLP markers
that have high discriminatory power and are easy to use. Genotyping T. gondii isolates
with these markers has proven useful. Adapting of these markers will facilitate
epidemiological and population genetic studies in T. gondii in the future.
Keywords: PCR RFLP, T. gondi, enzym restriction, DdeI.
138
The Effectiveness of API® 20NE Kit for Pasteurella multocida
Identification
Muhammad I. Desem1* , Didik T. Subekti1, and Susan M. Noor1
1Indonesian Research Center for Veterinary Science, Bogor 16114, Indonesia

*
Corresponding author: muhammad.desem@tutanota.com
ABSTRACT
Pasteurella multocida is a Gram-negative bacteria that classically can be identified by

conventional biochemical tests. However, these tests are less efficient and the test
results are sometimes inconsistent due to variations in the reaction. The API® 20NE kit
is considered capable of covering these weaknesses because it is more efficient and
effective in identifying P. multocida. This study aims to test the effectiveness of API®
20NE kit in identifying P. multocida. Three P. multocida isolates were used in this study.
Each isolate was characterized using API® 20NE kit and conventional biochemical tests.
The results showed that API® 20NE kit was only able to identify common P. multocida
strains and it was unable to identify certain P. multocida strains which had unusual
biochemical characteristics. This indicated that API® 20NE kit is not hundred-percent
effective for P. multocida biochemical identification.
Keywords: Pasteurella multocida, API 20NE, identification, biochemical,

characterization
139
Toxoplasmosis Molecular Detection Using Polymerase Chain Reaction
with 327 bp Primer Designed Based on Toxoplasma gondii B1 Gene
Aris Purwantoro1,3 , Wayan Tunas Artama1 , Bambang Sumiarto2 , Adi Heru

Sutomo3 , Rini Widayanti1 , Agung Budiyanto4 , Widagdo Sri Nugroho2 , Elsa
Herdiana Murhandarwati5 , Agustin Indrawati6

Mada
2Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas
Gadjah Mada
3Department of Public Health, Faculty of Medicine, Universitas Gadjah Mada
4Department of Reproduction, Faculty of Veterinary Medicine, Universitas Gadjah
Mada
5Department of Parasitology, Faculty of Medicine, Universitas Gadjah Mada
6Department of Microbiology, Faculty of Veterinary Medicine, Bogor Agricultural
University
ABSTRACT
Toxoplasma gondii infection causes toxoplasmosis which zoonotic for both humans and
animals. Infected livestock also looses their reproductive performances which are very
detrimental for farmers. In order to detect the infected patients without clinical
symptoms above, their deoxyribonucleic acids (DNA) have to be amplified using
polymerase chain reaction (PCR). The technique based on the protozoan B1 gene is
accurate and sensitive. The study aims to detect toxoplasmosis of goat meats based on
the protozoan B1 gene using a mini-thermocycler. The detection above using a
commercial kit was compared to self-designed primers using single-step PCR. Thirty goat
meat samples were purchased from satay kiosks throughout Kulon Progo Regency,
Indonesia. The samples were extracted its DNA then amplified using specific primers of
the protozoan B1 gene. The PCR products were electrophoresed then illuminated the
DNA bands by ultra-violet light. The study shows that the commercial kit and self-
designed primers illuminated 406 and 327 base pairs of DNA bands that specific for
toxoplasmosis detection. Moreover, the self-designed primers has an excellent
agreement (Kappa value 86.11%) compared to the comercial kit. The self-designed
primers has 94.44% sensitivity and 91.67% specificity that means it could be used for
further molecular detection.
Keywords: toxoplasmosis; molecular detection; polymerase chain reaction;

Toxoplasma gondii; B1 gene
140
Evaluation Of The Utilization Of Gra7 & And Sag1 Genes To Predict
Virulence Of Toxoplasma Gondii
Didik T Subekti1, Fitrine Ekawasti1, Zul Azmi1, Muhammad I Desem1
1 Indonesian Research Center for Veterinary Science, Bogor
ABSTRACT
Toxoplasma gondii is divided into three clonal populations that have different virulence.
Generally, the virulence differentiation is done by BioAssay using mice or by RFLP. This
study evaluates the use of the GRA7 and SAG1 genes to predict the clonal population.
This study used 24 strains of Toxoplasma gondii which were accessed and downloaded
from the genebank (NCBI Data Base). The data used are only the full length coding
sequence (full length CDS) from GRA7 and SAG1. Each of them was then aligned and
made a cladogram with the UPGMA method using the CLC Sequence Viewer 8. The
results of the cladogram showed that the GRA 7 CDS was able to form three virulence
clusters according to RFLP or BioAssay data. Only 2/24 (8.3%) of the strains were
misplaced. On the other hand, CDS SAG1 failed to form three virulence clusters and
therefore could not be used to predict virulence of Toxoplasma gondii.
Keywords: GRA7, SAG1, cladogram, CDS, UPGMA
141
142
Evaluation Nutrient Content of Liquid Organic Fertilizer of Rabbit’s
(Oryctolagus cuniculus) Urine Fermented by Various Bio-activator
Rachmad Dharmawan1 , Pratama Wahyu Adi P2
1Assessment Institute for Agricultural Technology, Denpasar, 80222

Bali, Indonesia.
2Faculty of Animal Science, University of Brawijaya, Malang, 65145,
East Java, Indonesia
ABSTRACT
Rabbit can produce urine 25-100 mL/day and soft fecal 28 g/kg body weight/day. The
nutrient content in rabbit urine was potential to be used as an alternative to liquid
organic fertilizers. The study aimed to evaluate the quality of rabbit urine liquid fertilizer
with the addition of various bio-activators. T0: commercial bio-activators, T1: fecal
extracts; and T2: Zingiberaceae extract. Anaerobic fermentation was carried out for 4
weeks with 3 aeration times: 0; 24, and 48 hours. The variables were pH, C-organic, N-
organic, C/N ratio, P2O5, and K2O. Data were analyzed using Factorial Completely
Randomized Design with Duncan's Multiple Range Test (DMRT) at 5% level. The results
showed that addition of various bio-activators showed significant differences against C-
organic, N-organic, and K2O (P<0.05), except pH, C/N ratio, and P2O5 (P>0.05). Aeration
time gives a significant difference to all variable (P<0.05), except P2O5, and K2O (P>0.05).
The nutrient quality at T1 was higher than T0 and T2 with the aeration time for 24 hours
giving the best results compared to 0 and 48 hours. It can be concluded that the addition
of cow fecal extract bio-activator with an aeration time of 24 hours can potentially be
used to replace commercial bio-activators.
Keywords: Anaerob, Aeration, Decomposer, Fermentation, Fecal, and Zingiberaceae.
143
Potential of Goat Manure as organic fertilizer in North Sumatera
Siti Fatimah Batubara*, Agung Budi Santoso, and Khadijah EL Ramija
Assessment Institute for Agricultural Technology (AIAT) North Sumatera

Jl. AH. Nasution No 1 B. Medan. 20143
*Email : sifa.cha@gmail.com
ABSTRACT
North Sumatra is the fifth largest province of goat population in Indonesia after Central
Java, East Java, Lampung and West Java. Solid and liquid goat manure has the potential
as a source of organic fertilizer. This study aims to obtain an overview of the potential
of goat manure as a source of organic fertilizer and its role in improving soil quality,
growth and crop production. The results showed that goat manure has great potential
to support an environmentally agricultural system. The case study in Deli Serdang
District showed that the application of fermented goat manure combined with balanced
inorganic fertilizers increased rice productivity by 18% and red chilies by 46%.
Keyword : goat manure, organic fertilizer, North Sumatera
144
Farmers Empowerment through managing organic waste as fertilizer in
Camplong II Village, Kupang Regency
Cynthia Dewi Gaina,1 , Maxs U.E. Sanam1, Frans Umbu Datta2, Filphin Adolfin Amalo1,
Tarsisius Considus Tophianong1 and Yohanes TRMR. Simarmata1
*1Faculty of Veterinary Medicine, University of Nusa Cendana, Kupang, Indonesia

2 Faculty of Animal Science, University of Nusa Cendana, Kupang, Indonesia
ABSTRACT
Camplong II is one of villages in Kupang regency that dominates by cattle farmer groups.
However, farmers has limited knowledge and skill in managing animal waste. The habits
of local farmers would likely produce negative impact on the environment by burning
organic material that can be used as source for organic fertiizer. This community service
aims to improve the knowledge and skills of trained farmers in making organic fertilizer
from organic and natural compound that is resulted from animal manure. The purpose
of the implementation of this community service was the manufacture of organic
bokashi, in which farmers were actively participated in this program. The results of this
training were organic fertilizers and vegetable products. Community service activities
have provided positive benefits in the form of increased knowledge about the benefits
of organic fertilizer and skills of producing bokashi fertilizer. Other important aspects
that are affecting this community are level of education, role of farmers level and source
of information. The final product has provide benefit impact on improving family’s
income.
Keywords: Empowerment, Community, Camplong II, Cattle, Farmers
145
Effects of Biofertilizer Application on Growth Rate and Chlorophyll
Content of Spinach (Amaranthus tricolor L.) in Salinity Stress Condition
Okky Shavira Riesty1 and Dwi Umi Siswanti2,1
1Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika

Selatan, Sekip Utara Bulaksumur 55281, D.I. Yogyakarta, Indonesia
2Laboratory of Plant Physiology, Faculty of Biology, Universitas Gadjah Mada, Jl.
Teknika Selatan, Sekip Utara Bulaksumur 55281, D.I. Yogyakarta, Indonesia
ABSTRACT
Amaranthus tricolor L. is one of vegetable that is widely consumed and cultivated. One
of land that can be used for agriculture is coastal land. But, the salinity conditions in
coastal land must be considered so that plant can growth well. One of the solutions to
help the growth of plants that grow under salinity stress is by applying fertilizers, one of
which is by applying biofertilizer, a fertilizer which is made from livestock urine and
microbial strains. In this research, the biofertilizer was given at a dose of 0, 10, 20, and
30 liter/ha and the stress treatment given was NaCl with a concentration of 0, 2500,
5000, 7500, and 10000 ppm. The average yield of growth rate, especially at the rate of
increase in plant height and number of leaves, the highest result is shown in the
treatment given biofertilizer treatment of 30 and 0 liters/ha. The highest mean total
chlorophyll content was shown in plants given a dose of 30 liters/ha. Based on the results
obtained, it is known that the application of biofertilizer affects the growth rate and
chlorophyll content of spinach, but only at certain concentration on certain salinity
levels.
Keywords: Fertilizer, Growth Rate, Salinity, Spinach
146
Utilization Of Agricultural And Livestock Waste
And The Effect On New Rice Varieties Yields On Rainfed Rice Field Of
Ponjong-Gunungkidul
Damasus Riyanto
BPTP Yogyakarta
Email : damasusriyanto@gmail.com
ABSTRACT
Environmentally friendly of agricultural land management with the application of liquid

organic of local microorganisms is believed to be able to maintain soil fertility, increase
the soil microbial population, preserve the environment and at the same time increase
soil productivity. The results showed that the application of organic fertilizers from
agricultural and livestock waste and the application of liquid microorganisms was able
to increase the Azospirillum population almost four times and the Azotobacter
population and Phosphate solvent microbes as much as two times as much on the soil
rhizosphere. This study aims to determine the application of various local
microorganisms (LMO) to the growth and yield of new rice varieties on the area of
Gunungkidul regency. The research took place from February to May 2017 in rainfed
ricefields, Ponjong District, Gunungkidul Regency. The research design used was
Randomized Completely Block Design (RCBD) with 4 replications and 6 different
treatments, namely: M1 = Local Microorganisms of vegetable waste, M2 = Local
Microorganisms of fruit waste, M3 = Local Microorganisms from banana weevils, M4 =
Local Microorganisms from urine and cow manure, M5 = Mol from Gliricidia sp leaves,,
M6 = a mixture of the five microorganisms ingredients. The results showed that the
Inpari 33 variety showed a higher yield of Harvest Dried Grain, namely 7.78 tons / ha
than the Inpari Sidenuk and Inpari 19 varieties, namely 6.93 tons / ha and 7.13 tons / ha,
respectively. The observations of yield components on the number of grains per panicle,
the number of fulled grains per panicle, 1000 grain weight and plant biomass weight
showed significant and higher differences in Inpari 33 varieties with application of mixed
local microorganisms (M6) compared to Inpari Sidenuk and Inpari 19 varieties with the
application of the five ingredients another local microorganisms. In addition, the
number of microbial populations in the M6 treatment was the most highest then
followed by the M4 treatment.
Keywords : Local microorganisms, new rice varieties, agricultural waste, Land

productivity
147
Sustainability and feasibility assessments of nanobubble aeration
technology in environment-economic-socio aspects of Penaeus
vannamei shrimp farming
Lily Susanti1, Suyud Warno Utomo1,2 , and Noverita Dian Takarina3
1School of Environmental Science, Universitas Indonesia, Jl. Salemba Raya 4, Jakarta

10430, Indonesia
2Environmental Health Department, Faculty of Public Health, Universitas Indonesia,
Depok 16424, Indonesia

3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas
Indonesia, Depok 16424, Indonesia
ABSTRACT
Penaeus vannamei shrimp farming in Indonesia is challenged by several issues in

environment, economic and socio aspects. This study aims to assess the benefits of
novel nanobubble aeration systems for ecosystem, business and community.
Experimental research was used by comparing P. vannamei post larva 10 reared at
density of 2000 inds./L in pond treated with nanobubble and pond without treatments
(control). Calculations of Net Present Value (NPV) and payback period were used to
assess the economic feasibility of nanobubble, while community interviews were used
to assess the feasibility in social sectors. Based on the results, environmental wastes of
shrimp farming in the form of total ammonia nitrogen (TAN) were reduced by 9% from
2,58 mg/l (95%CI: 0,91, 4,25) in control, compared to 2,35 mg/l (95%CI: 0,86, 3,84) in
treatment. According to the revenues obtained from selling post larva for five years and
investment costs for nanobubble, the estimated nanobubble NPV was IDR 64,824,374
with payback period of 1,7 year. The interviews on traditional shrimp farmer community
showed an agreement on the use of nanobubble aeration to support the livelihoods of
farmers among 61.1%-72.2% community members. To conclude, the use of nanobubble
is feasible to support sustainable P. vannamei farming.
Keywords: nanobubble, Net Present Value, payback period, shrimp
148
Utilizing Livestock Manure by Anaerobic Fermentation to Produce
Organic Fertilizer for Cultivating Low Glycemic Rice
Aris Purwantoro1,5,*, Ariana2, Trini Susmiati1, Eko Wismo Winarto3, Trisakti

Haryadi4

Mada
2Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada
3Department of Mechanical Engineering, Vocational School, Universitas Gadjah Mada
4Department of Livestock Socio-Economics, Faculty of Animal Science, Universitas
Gadjah Mada
5One Health Collaborating Center, Universitas Gadjah Mada, Yogyakarta, Indonesia
*Corresponding author: arispurwantoro@ugm.ac.id
Abstract
Indonesian farmers have various problems regarding livestock manure and chemical
fertilizer. The manure mostly keep beside barns without any treatment and pollutes its
surrounding environment. Prolonged usage of chemical fertilizer reduces paddy-field
fertility and might interfere human health. In addition, the International Diabetes
Foundation (IDF) states that 10 million people suffer from diabetes and Indonesia ranks seventh
in the world. Furthermore, the Indonesian Ministry of Health records the national prevalence
affected diabetes increased 0.2% per year and reaching 6.9% last year. The study aims to solve
the problems of livestock manure and human health above in synergistic and simultaneous
way. The manure was fermenting anaerobically using a biogas digester. The digester
constructed a 5 cubic-meter fixed-dome assembled of high-density polyethylene (HDPE) tank.
The process not only shortens its fermentation in just 14 days to produce organic fertilizer but
also generate biogas as renewable energy. The fertilizer was used to cultivate “Ciherang” rice
which has a low glycemic index (GI: 54). It can be concluded that anaerobic fermentation of
livestock manure could produce organic fertilizer and biogas. The fertilizer could be used to
cultivate low glycemic index rice which is healthy food for normal human and mostly suitable
for diabetic patients.
Keywords: anaerobic fermentation, livestock manure, organic fertilizer, low glycemic

rice
149
150
Phytochemical Compound and Acute Toxicity Test of Carica papaya
Leaves
Tahara Dilla Santi1,2, Tongku Nizwan Siregar1,*, Amalia Sutriana3, Rita Andini4
1Graduate School of Mathematics and Applied Science, Universitas Syiah Kuala, Jl.
Tgk. Syech Abdul Rauf, Darussalam, Banda Aceh, 23111, Indonesia
2Faculty of Public Health, University Muhammadiyah Aceh, Leung Bata,
Banda Aceh, 23245, Indonesia

3Faculty of Veterinary Medicine, Syiah Kuala University, Aceh 23111, Indonesia
4Forestry Department, Faculty of Agriculture, Syiah Kuala University, Darussalam,
Banda Aceh 23111, Aceh, Indonesia

* Corresponding author: siregar@unsyiah.ac.id
Abstract.
Introduction: Aceh has a wealth of flora including C.papaya L which contains secondary
metabolites and is used as traditional medicine. This study aims to obtain the
phytochemical compound of ethanol extract of C.papaya L leaves and an acute toxicity
test of Rattus novergicus. Materials and Methods: This research is an experimental study
with a post-test only design group. The acute toxicity test used 20 Wistar male rat which
was divided into four groups, namely the control group (K), the dose 250 kg BW group
(K1), the dose 500 kg BW group (K2), and the dose 1000 kg BW group (K3). C.papaya L
leaves extract solution was given orally every day for 14 days. Observations [i]include
hanging activity and platform testing observed for 24 hours starting from 0 minutes, 30
minutes, 1 hour, 2 hours, 4 hours, 7 days to 14 days. Result The results showed that the
leaves of C.papaya, contain flavonoids, alkaloids, steroids, saponins. Result: The LD50
value of extract ethanol of C.papaya was higher than 1000 mg/kg BW. There was no
toxic effects spectrum on Wistar rats during research. Discussion: Efforts to develop
traditional medicine can be done through the in vitro system by testing the acute toxicity
given in different doses. The identification and measurement of experimental animals
can predict the dangers and toxic properties of leaf metabolites. Conclusion The results
showed that ethanol extract of C.papaya leaves has secondary metabolites that can
have therapeutic effects. The C.papaya was a practically nontoxic substance and no
acute significant toxicity on experimental animals.
Keywords: Carica papaya leaves, phytochemicals compound, acute toxicity test.
151
The Effect of Colistin Administration as Medicated Feed on Alanine
Aminotransferase and Creatinine Level in Broiler Infected with
Escherichia coli
Aisah Nurul Fitri1, Ida Fitriana1, Antasiswa Windraningtyas Rosetyadewi1, Anggi

Muhtar Pratama1, Aria Ika Septiana1, Dwi Cahyo Budi Setiawan1 , Agustina Dwi
Wijayanti1
,1Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Gadjah

Mada, Yogyakarta, Indonesia
ABSTRACT
Colistin is a decapeptide antibiotic with narrow spectrum activity, mainly used as

treatment for Gram negative bacteria. This study aims to scientifically determine the
effect of colistin administration as medicated feed on alanine aminotransferase (ALT)
and creatinine level in broiler infected with Escherichia coli. KTOP group as positive
control, KTON group as negative control, while I, II, and III groups were infected with
Escherichia coli 1 x 108 CFU/ml 0.1 ml via intratracheal route. Group I, II, and III were
given colistin treatment dosage of 0.3 g/kg food, 0.6 g/kg food, and 1.2 g/kg food. Blood
samples were taken through brachial veins for ALT and creatinine examination with a
Caretium NB-201 semi-auto chemistry analyzer. Data were examined statistically using
IBM SPSS Statistics 24 software and graphically using Microsoft Excel 365. Conclusion of
the research by statistical analysis with Kruskal-Wallis test obtained ALT test results P =
0.147 and creatinine test results P = 0.815. Based on the results of this study, the
administration of colistin medicated feed did not cause a significant effect on ALT and
creatinine level in broiler infected with Escherichia coli, so that colistin has low potential
toxicity while given as medicated feed.
Keywords: ALT, broiler, colistin, creatinine, medicated feed.
152
Efficacy of Aloe Vera (Aloe barbadensis) Extracts as Extender for
Cryopreservation of Sheep (Ovis aries) Semen
Almeron Amiel John Edward1, Beltran Ma. Asuncion1, Francia Allan Jeffrey1,
Lacayanga Charlie1, Villarama Regina1
1College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac,

Philippines, 2306
ABSTRACT
Aloe vera (Aloe barbadensis) is native in Canaries, Southern Europe, and North Africa.
And is grown in tropical countries, like Philippines. It is consisted of most of the bioactive
compounds. Because of its chemical compositions and properties, this study aimed to
determine the efficiency of aloe vera extracts as extender for cryopreservation of sheep
semen. Twelve semen samples were collected from three apparently healthy buck ages
1.5 to 3 years old which are regularly used for breeding. The semen samples were
divided and distributed in different extender treatments (Treatment 1 (Tris Egg-Yolk
Extender), Treatment 2 (10% aloe vera gel extender), Treatment 3 (20% aloe vera gel
extender), and Treatment 4 (30% aloe vera gel extender). The semen was evaluated
before and after cryopreservation at 7th, 14th, and 21st day of post-thawing. The result
demonstrated that the extender with 10% aloe vera extracts showed the highest post-
thaw motility. The sperm cryopreserved with egg-yolk extender and 30% aloe vera
showed the highest percentage of acrosome integrity. However, high sperm livability
was observed using egg-yolk extender. Statistically, there is no significant differences
among treatments in cryopreserving sperm. Thus, aloe vera extracts can be used in
cryopreservation of sheep semen.
Keywords: Aloe vera, cryopreservation, egg yolk, extender, sperm
153
The Cytotoxic Activity of Pine Needles Ethanolic Extract of Pinus merkusii
on HeLa Cell Lines
Annise Proboningrat1, , Amaq Fadholly1, Sri Agus Sudjarwo2, Fedik Abdul Rantam3,4,5,
and Agung Budianto Achmad6
1Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas

Airlangga, 60115 Surabaya, Indonesia
2Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga,
60115 Surabaya, Indonesia

3Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga,
60115 Surabaya, Indonesia

4Institute of Tropical Disease, Universitas Airlangga, 60115 Surabaya, Indonesia
5Stem Cell Research and Development Center, Universitas Airlangga, 60115 Surabaya,
Indonesia
6Department of Health, Faculty of Vocational Studies, Universitas Airlangga, 60115
Surabaya, Indonesia.
ABSTRACT
Efforts have so far been made to discover new anticancer agents based on natural
ingredients. Several studies have reported that several Pine genus species exhibit
cytotoxic activity against various types of cancer cells. This plant is rich in phenolic
compounds, especially procyanidins, other flavonoids, and phenolic acids. In this study,
we investigated the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer
cell lines. The cytotoxicity assessment was measured by MTT assay and expressed as IC50
value. This study revealed that the ethanolic extract poses a dose- and time-dependent
cytotoxic activity, with the best IC50 value of 542,5 µg/ml at 48 hours of incubation time.
In conclusion, Pinus merkusii needles' ethanolic extract has potential as a novel
candidate for an anticancer agent.
Keywords: Anticancer, Pinus merkusii, Cervical cancer, Cell viability.
154
Effects of probiotic and sodium diformate on productivity and immunity
of broiler chickens
Aria Ika Septana 1 , Agustina Dwi Wijayanti1, Antasiswa Windraningtyas R.1, Anggi
Muhtar Pratama1, and Ida Fitriana1

Mada Yogyakarta, Indonesia
ABSTRACT
The production of broiler in Indonesia before 2018 were very identical to the use of
Antibiotic Growth Promoter (AGP). Natural compounds and minerals are very likely to
replace the function of AGP. The mineral that has been developed as an additive to
chicken feed is sodium diformate because it is thought to improve the performance of
the digestive system and reduce pathogenic bacteria. Probiotic, consisting of several
bacteria (Lactobacillus and Enterococcus) which are normal microbes in the digestive
tract of poultry were used to improve the broilers performance. The aim of this study
was to examine the effects of minerals and probiotics and the combination on
productivity and immunity of broilers. The results showed that the administration of
sodium diformate and probiotics were increased the feed conversion ratio in broiler
chickens (1.34±0.05) and better than standard (less than 1.5) and significantly difference
between group. The provision of mineral and probiotics also has an impact on increasing
immunity, marked by the antibody titer value against Newcastle Disease (Day 18:
9.83±7.4, Day 28: 14.40±10.18). The provision of sodium diformate and probiotics
combination has generally shown good results to increase the productivity and
immunity of broiler.
Keywords: sodium-diformate, probiotic, broiler, produvtivity, immunity
155
In Vitro Anthelmintic Activity of Clove Leaf Extract (Syzygium
aromaticum) Against Ascaridia galli
Santika Anggrahini1 , Irkham Widiyono2 , Soedarmanto Indarjulianto2 , and Joko

Prastowo3
1Student of Doctoral Program Veterinery Science, Faculty of Veterinary Medicine,

Universitas Gadjah Mada, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia
2Department of Veterinary Interna Medicine, Faculty of Veterinary Medicine,
Universitas Gadjah Mada, 55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia

55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia.
ABSTRACT
This study aims to determine the anthelmintic potential of clove leaf ethanol extract
(CLE) against Ascaridia galli in vitro. A total of 240 worms were divided into 8 groups,
CLE was prepared in seven different concentrations (20, 40, 60, 80, 100, 120, and 140
mg/ml) and saline water as control, respectively with 3 repetitions. Ten worms of each
group submerged into 25ml of the tested solution for 3-9 hours, then evaluated using a
Scanning Electron Microscope (SEM). The results showed the highest mean mortality at
3h, 6h, and 9h observation was CLE 140 mg/ml. The ethanol extract of clove leaves
caused surface changes with damage to the cuticle of A. galli adult worm. It concluded
that CLE possess anthelmintic activity.
Keywords: clove leaf, Ascaridia galli, anthelmintic, in vitro
156
Comparative Characteristic Study from Bone Marrow-derived
Mesenchymal Stem Cells
Medania Purwaningrum1,2, Nabila Syarifah Jamilah1, Steven Dwi Purbantoro1,

Chenphop Sawangmake1,3,4, Sirirat Nantavisai1,3,*
1Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary

Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science,
Chulalongkorn University, Bangkok 10330, Thailand

3Veterinary Clinical Stem Cell and Bioengineering Research Unit, Faculty of Veterinary
Science, Chulalongkorn University, Bangkok 10330, Thailand

4Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn
University, Bangkok 10330, Thailand

* Corresponding author: nongvet1984@hotmail.com
ABSTRACT
Tissue engineering has been extensively investigated and proffered to be a potential

platform for novel tissue regeneration. The utilization of mesenchymal stem cells (MSCs)
from various resources have been widely explored and compared. In this regard, MSCs
derived from bone marrow have been proposed and described as the promising cell
resource due to high yield of isolated cells which contain the potential of colony forming,
self-renewal, MSCs surface marker expression and multilineage differentiation
capacities in vitro. However, various supporting evidences of bone marrow
mesenchymal stem cells (BM-MSCs) both in vitro and in vivo from different species
presented the identical and unique potential of stemness characteristics. In this review,
the fundamental knowledge of the growth kinetics and stemness properties of BM-MSCs
in different animal species and human is compared and summarized. Finally, to
accomplish its full prospective, this review will procure the current information studies
focusing for the implementation of BM-MSCs in clinical practice.
Keywords: mesenchymal stem cells, bone marrow mesenchymal stem cells, stemness
characteristics
157
Effect of Natural Aromatase Blocker Towards Adult Pelung Chicken
Rizki Fitrawan Yuneldi1, Pudji Astuti2, Hendry T Saragih3, and Claude Mona Airin2,*
1
Veterinary Science Doctoral Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Indonesia
2
Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
3
Faculty of Biology, Universitas Gadjah Mada, Indonesia
*Corresponding author : monaairin@ugm.ac.id
ABSTRACT
The study aimed was to determine the effect of natural aromatase blocker (NAB) on comb growth,
crowing percentage, weight, and the testicular ratio of adult Pelung chicken. Control data were taken
before treatment (day 0) and treatment with blood clamshell powder (Anadara granosa) for 8 weeks.
Measurement of data on comb growth and recordings of Pelung cockcrowing was carried out every 2
weeks for 8 weeks. Pelung cock crows were recorded using the Hegemon Touch method and analyzed
using the Audacity application. The quantitative data that has been obtained were analyzed using one-
way ANOVA at the 95% level of confidence with the help of SPSS version 15 software. The results
showed that there was an increase in adult Pelung's comb growth but not significantly different (P >
0.05) from week 2 to week 8 with a range between comb length 6.52±0.72 to 7.45±1.52 and comb
height 13.09±1.11 to 13.77±1.27. The percentage of the song was higher than call, testicular weight
and ratio were still in the normal range. It can be concluded that giving NAB to adult Pelung chicken
can increase comb growth, but it is not significant and the percentage of chicken crows is higher in
song.
Keywords: comb, crowing, natural aromatase blocker, pelung, testes
158
Chemotherapeutic Ability of Ocimum sanctum ethanolic extracton the
Human Lung Adenocarcinoma (A549)
Ulayatul Kustiati1, Dwi Liliek Kusindarta1, Hevi Wihadmadyatami1*

ABSTRACT
Lung cancer is one of the causes of death because of cancer. In the world, lung cancer
causes reached 11.6% of cases and caused 18.4% of deaths. Current therapy still has
adverse side effects on normal therapy cells. In the last decade, researchers have begun
to focus on the role of traditional medicine as an alternative and complementary
medicine for cancer treatment. Ocimum sanctum (OS) is an herbal plant that is easy to
find and is widely used as alternative medicine in Indonesia. Previous research has
shown that OS extracts have therapeutic properties and, in some cases, antitumor
properties. This study will analyze OS ethanol extract's ability to inhibit proliferation,
migration, and vasculogenesis of A549 cells. MTT test, CCK-8 test as well as in silico
analysis was applied. Our data showed, the ethanol extract of OS significantly inhibited
the viability of A-549 cells, down-regulated the expression of α5β1 integrins, αvβ3
integrins, and VEGF. Our findings suggest that the ethanolic extract of OS interferes with
the angiogenesis of A549 cells. This phenomenon lead by disruption of cell migration
and proliferation as a consequence of downregulation of α5β1 integrins, αvβ3 integrins,
and VEGF. Overall, the ethanolic extract of OS may represent a promising therapeutic
candidate for treating metastases in pulmonary adenocarcinoma. More research is
needed to establish further the efficacy of OS in the treatment of pulmonary
adenocarcinoma.
Keywords: A549, integrin α5β1, integrin αvβ3, lung cancer. VEGF
159
Bovine Umbilical Vein Endothelial Cells Conditioned Medium (BUVEC-
CM) as Anti-Inflammatory Agent on the in-vitro Model
Neurodegeneration Induced by TMT
Vinny Anisya Larasati1, Gregorius Viktor Lembang1, Dwi Liliek Kusindarta1, and Hevi
Wihadmadyatami1

Yogyakarta, 55281
*Corresponding author: heviwihadmadyatami@ugm.ac.id
ABSTRACT
Neurodegenerative diseases were considered as degeneration of structure and function

either on the central or peripheral nervous system. Conditioned medium (CM)
containing secretory products, used in cell-free therapy, represents an alternative to
cell-based therapy with the advantage of lower risks, the possibility of allogeneic
administration, and large scale production. In the present study, we hypothesized that
the paracrine effects of Bovine Umbilical Vein Endothelial Cells Conditioned Medium
(BUVEC-CM) might promote neuronal regenerative and act as an anti-inflammatory on
the in-vitro model of neurodegeneration. BUVEC-CM was analyzed by High-Resolution
Mass Spectrometry (HR-MS), and some others were treated into PC12 cell line in the
presence of TMT and Donepezil HCl as a comparison. Besides, to prove this postulated,
we also performed in-silico modeling. Our data shows the BUVEC-CM promote
proliferation of the PC12 and downregulate several inflammatory factors.
Keywords: neurodegenerative disease, BUVEC-CM, PC12 cell line, inflammation
160
Conditioned Medium of E17 Rat Brain Cells Induced Differentiation of
Primary Colony of Mice Blastocyst into Neuron-Like Cells
Budiariati Vista1,*, Rinendyaputri Ratih2, Noviantari Ariyani2, Haq NMD3, Budiono

Dwi3, Pristihadi DN3, Juliandi Berry4, Fahrudin Mokhamad3, Boediono Arief3
1 Department of Anatomy Faculty of Veterinary Medicine, Universitas Gadjah Mada,

2Center for Research and Development of Biomedical and Basic Health Technology,
National Institute of Health Research and Development, Ministry of Health Republic

of Indonesia, Jakarta, Indonesia
3Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary
Medicine, IPB University, Bogor, Indonesia

4Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University,
Bogor, Indonesia
* Corresponding author: vista.budiariati@ugm.ac.id
ABSTRACT
Conditioned medium is the medium obtained from certain cultured cells and contained
secretome from the cells. The secretome which can be in the form of growth factors,
cytokines, exosomes, or other proteins secreted by the cells, can induce differentiation
of the cells that still have pluripotent or multipotent properties. The conditioned
medium used in this study originated from E17 rat brain cells. The CM was used to
induce differentiation of primary colonies of mice blastocysts. Primary colonies were
stained with alkaline phosphatase to analyze the pluripotency. We examined the
morphological changes of the colonies and stained the colonies on day 2 and 7 after
the addition of conditioned medium using GFAP and NeuN markers. We found that the
conditioned medium could differentiate the primary colony, beginning with the
formation of an embryoid-bodies like structure, then we identified round shape GFAP
positive cells, and finally, there were neuron-like cells which positive for Neu-N on
seventh days after the addition of the conditioned medium. Conditioned medium from
different species, in this case, E17 rat brain cells, induced and promoted differentiation
of the primary colony from mice blastocyst into neuron-like cells. The addition of CM
mediated neurite growth in the differentiation process.
Keywords: blastocyst, conditioned medium, differentiation, primary colony, rat brain

cells.
161
162
The Effect of Daun Wungu [Graptophyllum pictum (L.) Griff] Ethanol
Extract on Glucose Tolerance and Pancreas Islets of Langerhans in
Ovariectomized Mice
Listijani Suhargo , Diana N.Chandra , and Dwi Winarni
Department of Biology, Faculty of Science and Technology, Universitas Airlangga,

Surabaya, Indonesia
ABSTRACT
This research was aimed to determine the effect of daun wungu [Graptophyllum
pictum (L.) Griff] ethanol extract on glucose tolerance and pancreas islets of
langerhans in ovariectomized mice. Ovariectoalmy was first performed in all
experimental female mice. There are six groups in this research, namely, the
normal control group (0.2ml aquadest), the ovariectomized groups which
included the control group (0.2ml aquadest), the hormonal control groups
(0,2ml esthero solution) and the treatment groups with daun wungu ethanol
extract (10 mg/kg, 20 mg/kg and 30 mg/kg ). After the end of the research all
mice were tested for glucose tolerance, sacrificed and the pancreas organ was
cut off to make pancreas slide with Hematoxyllin, eosin The pancreas histology
was made using the paraffin method and Haematoxylin-Eosin staining. The
results of this research shew that daun wungu ethanol extract decreased
glucose tolerance value and increase the diameter of pancreas islet of
langerhans. And the optimal dose of daun wungu ethanol extract was 20
mg/kg.
Keywords: Daun wungu, glucose tolerance, islet of langerhans, ovariectomized

mice.
163
Antimicrobial Resistance in Coagulase Negative Staphylococci Isolated
from Subclinical Mastitis in Ettawa Crossbred Goat (PE) in Yogyakarta,
Indonesia
Suwito Widodo1 , Srinugroho Widagdo2, Bambang Sumiarto2 and AETH Wahyuni3

1Assessment Intitutes for Agricultural Technology of Yogyakarta, JL Stadion
Maguwoharjo No 22, Wedomartani, Ngemplak, Sleman, Yogyakarta, 55584 Indonesia
2Department of Veterinary Publich Health, Faculty of Veterinary Medicine, Gadjah
Mada University, Jl. Fauna No 2, Bulaksumur, Yogyakarta 55281, Indonesia

3Department of Microbiology, Faculty of Veterinary Medicine, Gadjah Mada University,
Jl. Fauna No 2, Bulaksumur, Yogyakarta 55281, Indonesia
ABSTRACT
Subclinical mastitis (SCM) in Ettawa Crossbred Goat (PE) is the most frequent caused by
Staphylococci with significant reduction in milk yield. The present study was determined
antimicrobial resistance patterns Coagulase Negative Staphylococci (CNS) from PE goat
SCM. A total of 36 CNS isolates originating from PE goat SCM. All CNS isolates were
further examined for antimicrobial susceptibility testing by Kirby-Bauer disc diffusion
method. Antibiotic susceptibility of CNS isolated samples according to the standards of
Clinical Laboratory Standards Intitute. The CNS isolates showed the highest resistance
rate against sulfamethoxasole (65%), ampicillin (55.56%), penicillin (45%), cefoxcitine
(33.33%), erythomycin (25%), oxytetracycline (20%), tetracycline (15%), gentamicin and
neomycin 11.11%, while oxacillin is sensitive. The highest multiple antimicrobials were
observed 15% in ampicillin, penicillin and tetracycline, then 5-10% in ampicillin,
penicillin, erythromycin, tetracycline and oxytetracycline. Antimicrobial administration
in PE goat SCM especially which caused by CNS should be aware so that prevent SCM in
PE goat is more important rather than treatment with antimicrobial.
Keywords: antimicrobial, staphylococci, subclinical mastitis, goat.
164
Review: Canine Dermatophytosis in Indonesia, a Tropical Country,
Compared to the Non-tropical Countries
1 2 1
Yun Fen Tan , Yanuartono1, Sitarina Widyarini , Alfarisa Nururrozi , and
1*
Soedarmanto Indarjulianto
1
Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia.
2
Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia.
* Corresponding author: indarjulianto@ugm.ac.id
ABSTRACT
One of the disease that commonly affect dogs all around the world is dermatophytosis,
a skin disease caused by a group of fungi called dermatophytes. It is understand that
dermatophytes can be found in both the tropics and non-tropics. The aim of this review
was to explore the prevalence and causative agents of dermatophytosis in canine at
different regions. Articles on canine dermatophytosis from Indonesia and a few other
non-tropical countries were collected. The data obtained were tabulated and discussed
descriptively. The results showed that Indonesia 27/60 (45%) had almost two-fold
higher prevalence of canine dermatophytosis compared to the non-tropical countries
287/1,027 (27.95%). Microsporum canis was the main causative species found in
canine positive with dermatophytosis in both the tropics and non-tropics, followed by
Trichophyton sp. Following that, Microsporum gypseum was among the common
species found to infect dogs in the non-tropical countries. Other dermatophyte species
found in the non-tropics were Microsporum audouinii and Microsporum nanum.
Results obtained from this retrospective study give an illustration on the difference in
prevalence and causative agents of canine dermatophytosis in tropical and non-tropical
countries.
Keywords: Dermatophytes, dogs, tropics, non-tropics, prevalence.
165
Fish Disease Identification of Fresh Water fish in the Yogyakarta Special
Region, Indonesia
Yuli Purwandari Kristianingrum*, Bambang Sutrisno, Sitarina Widyarini, Kurniasih,

Sugiyono
*Department of Pathology, Faculty of Veterinary Medicine, UGM

Fauna Street, No.2 Karangmalang, Yogyakarta 55281
*Corresponding Author : yuli_purwandari@mail.ugm.ac.id
ABSTRACT
The potential of freshwater farming in the Special Region of Yogyakarta is very high. The
infectious and non-infectious disease can cause significant economic loss. The main
problem of infectious disease is caused by viruses, bacteria, fungi and parasites. This
study was conducted to identify of diseases that attack freshwater fish by data analysis
on fish disease cases in the Pathology Laboratory of the Faculty of Veterinary Medicine,
UGM in 2019. A total of 239 cases of fish diseases were examined by the Laboratory
Diagnostic Co-assistance students in 2019 from the Yogyakarta Special Region which
includes Sleman district, Kulonprogo district, Yogyakarta City, Bantul district and
Gunungkidul district. Based on data analysis, it showed that the highest fish sample was
tilapia (52%), comes from Sleman Regency (94%). The highest disease infection was
caused by bacterial infection (54%), parasite infection (35%). Macroscopic changes were
observed in the liver (25%), skin (19%). and gills (26%). These changes included a yellow
swollen liver, ulcer in the skin, fragile consistency and nodules in the surface of kidney,
attachment lamella and necrosis of gill lamellas. The parasite identification was found
Dactylogyrus sp, Trichodina sp., Gyrodactylus sp., Ichtyopthirius sp in the Parasitology
Laboratory. The histopathological changes by the Hematoxillin & Eosin staining
technique, the organs showed dermatitis (18%), hepatitis (27%) and branchitis (23%).
The conclusion of this research showed that the highest case of freshwater fish disease
in Yogyakarta Special Region was caused by bacterial infection of tilapia from the Sleman
district. .
Keywords: Freshwater fish, virus , bacteria, fungi, Yogyakarta Special Region
166
A Publication of Escherichia coli in Veterinary Medicine : Insights from
Scientometric Analysis (2011-2020)
Lailatul maghfiroh1
1 Master of Animal Disease Science and Veterinary Public Health, Univeristas Airlangga,
Indonesia
Corresponding author : lailatulmaghfiroh1210@gmail.com
ABSTRACT
Escherichia coli is a commensal bacteria in the digestive tract of humans and animals
that is very influential on health. There has been a lot of research on Escherichia coli, but
no studies have addressed the idea of publishing Escherichia coli in Veterinary Medicine
which shows the big overview using data from all countries. This study aims to map the
status of Escherichia coli publications in the field of Veterinary Medicine which are
published internationally and indexed by Scopus using bibliometric visualization.
Bibliometric methods and research data analysis were implemented using analysis
search results services from Scopus and VOSviewer applications. In this study, the details
collected apply to 5836 documents published from 2011 to 2020. Research reveals that
Kim, I. H., Universiteit Gent, and the United States are individual scientists, affiliated and
country most active in Escherichia coli publications on Veterinary Medicine. In the
publication Escherichia coli in Veterinary Medicine, Veterinary and Veterinary
Microbiology are the most subject area and sources of dissemination. There are six maps
of collaborative research groups in Escherichia coli publications in Veterinary Medicine
around the world. To identify a body of knowledge created from ten years of publication,
this study builds a convergence axis grouping consisting of Escherichia coli studies:
Immunology, Virulence Factors, Microbiology & Animal Molecular Examination
abbreviated with the theme IVMA.
Keywords: Escherichia coli, Veterinary, Bibliometric, Vosviewer
167
Comparison of Guinea Pigs Health Raised in Uncontrolled and Controlled
Environments
Laksmindra Fitria1, Nastiti Wijayanti2, Tuty Arisuryanti3 and Siti Isrina Oktavia
Salasia4.*
1 Doctoralstudent of Graduate Program in Faculty of Biology, Universitas Gadjah Mada,

Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
2 Laboratory of Animal Physiology, Department of Tropical Biology, Faculty of Biology,
Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, 55281,
Indonesia
3 Laboratory of Genetics and Breeding, Department of Tropical Biology, Faculty of
Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta,
55281, Indonesia
4 Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Jalan Fauna No. 2 Karangmalang, Yogyakarta 55281, Indonesia

*Corresponding author: isrinasalasia@ugm.ac.id
ABSTRACT
Guinea pigs (Cavia porcellus) are usually kept as a pet. They are also species commonly
used in biological and biomedical research such as rats and mice. Consequently,
understanding their health status is vital because it determines the quality of their life
and the validity of the research data. As pets or experimental animals, guinea pigs are
mainly sourced from animal markets supplied by traditional farms, where
environmental factors are not properly controlled to meet animals’ basic requirements.
This study was carried out to compare the health status of guinea pigs raised in
uncontrolled environment (conventional system) and moved to a controlled
environment (in the animal facility). Parameters observed included general health
condition, body weight, core temperature, complete blood count, evaluation of liver
function (ALT, bilirubin) and kidney function (BUN, creatinine), as well as ecto- and endo-
parasites. Results indicated differences in these parameters’ values of controlled and
uncontrolled environments, some of which were significant based on statistical analysis.
It can be concluded that care management predisposes the health status of guinea pigs.
Keywords: guinea pigs, hematology, clinical biochemistry, disease, care management
168
Mapping The Plague Focus Area with Temporal-spatial Analysis Based on
The Body Flea Index in Boyolali District
Rian Saputra1*, Widagdo Sri Nugroho2, Anis Fuad1, Teguh Tri Kuncoro3,
1
Departemen Biostatistik, Epidemiologi, dan Kesehatan Populasi FKKMK UGM, Indonesia
2
Fakultas Kedokteran Hewan UGM, Indonesia
3
Dinas Kesehatan Kabupaten Boyolali
ABSTRACT
Body flea index on R. rattus. diardi is one of the important indicators for the prevention
and control ofplague. This study aims to map the risk of plague on the plague foci of
Boyolali district based on the distribution pattern of body flea index on R. rattus. Diardi.
This study was an observational epidemiological study with a temporal-spatial approach.
A temporal-spatial approach is used to identify epidemiological data in the form of body
flea index that is geographically suspected to be directly or indirectly related to the
incidence of plague disease using the Arcgis application. In 2011 the distribution of body
flea index formed clusters pattern, while other years showed a random distribution. The
2010-2019 body flea index distribution shows a cluster and outlier distribution pattern.
The results of the Getis-Ord Gi hot spot analysis on the body flea index indicate that
every year, a hot spot region always appears. The villages that most often become hot
spots are Selo and Samiran villages. Body flea index on R. rattus. diardi shows spatial
and temporal patterns. The diversity of temporal and spatial distribution is also
influenced by seasonal variations, human activities, and natural factors. Information
related to distribution patterns and the hotspots areas of body flea index will help
support the decision making process to reduce the risk of plague.
Keywords: Plague, Flea index, Temporal Spatial, Cluster and outlier, Geographic
Information System
169
Case Report: Treatment of Feline Panleukopenia, Otitis and
Helminthiasis in a Cat
Soedarmanto Indarjulianto1*, Carolina Divita Ardaniputri2, Yanuartono1, Alfarisa

Nururrozi1, Hary Purnamaningsih1, Slamet Raharjo, and Puveanthan Nagappan
Govendan3
1Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah

Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia
2Dokter Hewan Praktisi. Jl. Bukit Palma no 5, Bukit Sari, Semarang
3V Care Klinik Haiwan, Kota Bharu, Kelantan, Malaysia.
ABSTRACT
Feline panleukopenia (FPL) is rarely accompanied by infection with other agents. This
case report describes the diagnose and treatment of a 6 months old male cat, one kgBW,
that diagnosed FPL with complication. The physical examination of cat showed lethargy,
diarrhea, capilarry refilling time > 2 seconds, body temperature 40.3℃, swollen of
sinister submandibular lymphoglandula, smelly discharge from the left ear and
increased intestinal peristaltic. The blood profile illustrating normocytic normochromic
anemia, thrombocytopenia, panleukopenia with lymphopenia, and neutropenia. Fecal
FPV-Ag-test revealed a positive for FPL. Fecal examination were observed eggs of
Ancylostoma sp., Dipylidium sp., and Toxocara sp. along with positive in the benzidine
test. The cat was diagnosed FPL, otitis and helminthiasis with a dubious prognosis.
Treatment was given for 6 days by intramuscular injection of 10mg/kgBW amoxicillin
bid, 2mg/kgBW diphenhydramine-HCl once a day, 0.1mL vitamin B complex once a day,
intravenous infusion of 20mL Ringer's lactate bid, Erlamycetyn ear drop bid and 1/4
tablet (18.2mg-praziquantel+72.6mg-pyrantel pamoate) once. The cat's health status
has improved after treatment, characterized by normal appetite and body temperature,
absence of ear fluid, normal feces and no finding of worm eggs as well as an
improvement of normal blood profile.
Keywords: feline panleukopenia, otitis, dipylidium, ancylostoma, toxocara.
170
The Effects Of Natural Aromatase Blockers On The Testicle Weight, Size
Of Wattle And Histopatological of Testis In Bangkok Chicken
Pudji Astuti, Claude Mona Airin, RR Amelia Hana, Sarmin
Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah

ABSTRACT
Aromatase enzyme is an enzyme that converts testosterone to estrogen. This means

that by administering aromatase blockers, Testosterone levels increase and estrogen
decreases. The problem faced is how the spermatogenesis process occurs because
estrogen is also very much needed in the spermatogenesis process. The aim of this
study was to evaluate the histopathological features of the testes due to the
administration of Natural Aromatase blockers (NAB).
Fifteen male Bangkok chickens were divided into 5 groups, namely: Control group (C);
Natural Aromatase Blockers 33 (NAB 3.3gr / bird); Natural Aromatase blockers 66
(NAB 6.6gr / bird); Testosterone continues (Tc): 0 , 4 mg / head/day; Zinc (Z): 0.018
mg / 40 g body weight. Oral treatment was given for 35 days. The results showed that
NAB did not affect wattle growth, but significantly affected testicle weight (p <0.05).
The NAB 66 group had the heaviest testis weight, followed by Zinc, Continuous
Testosterone, NAB 33 group and the control group. This was confirmed by HE staining
on the testes which showed the thickness of the thickest spermatogenic and
spermatid cell layers in the NAB 66 group. It was concluded that giving NAB did not
have a negative effect on the spermatogenesis process.
Keywords: NAB, Bangkok Chicken, spermatogenesis, testicle wight, wattle
171
Case Report: Diagnosis and Treatment of Enteritis Caused by Bacterial
in a Dog
1 2*
Mungky Ema Ramadhani , Soedarmanto Indarjulianto , Yanuartono2, Alfarisa
2 2 3
Nururrozi , Slamet Raharjo , and Alagenthirah Nakkeeran
1
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang,
2
3
Rimba Academy, No. 50, Jalan 2/87G, Taman Seputeh, 58000 Kuala Lumpur, Wilayah
Persekutuan, Malaysia.
ABSTRACT
Diagnosis of the cause of enteritis in dogs greatly influences the success of its
treatment. This case report describes the management of a male dog, 5 months old,
4.8 kg body weight which reported diarrhea, fever and no appetite. The physical
examination showed the dog had diarrhea, lethargy, anemic mucous membranes,
body temperature of 39.6 °C and an increase in intestinal peristalsis. The results of
blood tests showed normochromic microcytic anemia, decreased hemoglobin and
PCV, lymphocytopenia, and eosinopenia. The results of the stool examination
identified Escherichia coli, Aeromonas hydrophila and coliform. The dog is diagnosed
with bacterial enteritis with a good prognosis. Treatment is given for 5 days with
intramuscular injection of amoxicillin at a dose of 10 mg/kgBW bid, diphenhydramine
HCl at a dose of 2 mg/kgBW bid, multivitamin syrup 0.1 ml/kgBW bid orally, and
intramuscular injection of iron dextran at a dose of 10 mg/kgBW only on the fifth day.
It was concluded that the dog had enteritis due to bacterial infection, and it recovered
after 5 days of treatment.
Keywords: bacterial, dog, enteritis, Escherichia coli, Aeromonas hydrophila.
172
A Review: The Prevalence of Dermatophytosis on Cats in Indonesia and
Turkey
1 2 3 3
Fatin Hanani Md Zaki , Claude Mona Airin , Alfarisa Nururrozi , Yanuartono and
3*
Soedarmanto Indarjulianto
1
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2,
2
Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia.
3
ABSTRACT
Dermatophytosis (ringworm) is an important feline skin disease primarily caused by the

dermatophyte genera Microsporum and Trichophyton which may be affected by
climate. Studying the prevalence of dermatophytosis on cats in Indonesia and Turkey
was the main purpose of this article review. The articles reviewed were two articles from
Turkey published consecutively on year 2011 and 2016, and an article from Indonesia
published on year 2017. The findings showed that the prevalence of dermatophytosis in
Indonesia was 17/30 (56.7%) while Turkey was 52/428 (12.1%). The age of the infected
animals was the only predictor that had a major effect on the incidence of
dermatophytosis on cats, as shown by the excellent positive cases of cats under one year
of age relative to other age groups. In conclusion, the prevalence of dermatophytosis on
cats in Turkey was slightly lower compared to the prevalence of feline dermatophytosis
in subtropical country (Indonesia) and the variations may be attributed to the variation
in humidity and temperature faced in both countries.
Keywords: dermatophytosis, prevalence, cats, humidity, temperature
173
Moderate Intensity Activity As An Anti-Fatigue Agent
Aditya Candra
Department of Physiology, Faculty of Medicine, Abulyatama University, Aceh Besar 23372,

Indonesia
ABSTRACT
Muscle fatigue is a physiological response that occurs due to prolonged muscle

contraction resulting in reduced energy in ATP. The accumulation of lactate and protons
is the cause of fatigue. Aerobic exercise performed with moderate intensity with a pulse
rate of 70% - 80% HRMax can inhibit fatigue. Many publications am confirmation the
benefits of physical exercise that, if done properly and correctly, can prevent and treat
fatigue due to constant work. Physical activities so that we do not control quickly
become a trigger for fatigue that we cannot avoid. Excessive physical activity can trigger
fatigue, but if done properly, correctly, measured, and regularly, it can function the
opposite, namely to prevent fatigue. The correct role of physical exercise is closely
related to the beneficial cellular and metabolic effects in the body. In animal and human
studies, sure increase endurance exercise. Different methods of physical activity from
the intensity, duration of time, and frequency of exercise resulted in another level of
fatigue experienced. And the most that can impossible is a physical activity with
moderate intensity with a duration of fifteen minutes and carried out three times a week
with similar a physical activity. Research conducted on humans, it is proven that
moderate-intensity physical exercise, if done properly, correctly, measured, and
regularly can prevent, and inhibit fatigue describe by continued stable performance.
Heavy intensity express because it can cause the emergence of free radicals in the body.
Currently, various efforts have been made to overcome fatigue, both in silicon, in vitro,
and in vivo studies. Recent studies have shown that moderate-intensity physical activity
in various types of exercise can be a very promising anti-fatigue agent, besides being
able to inhibit fatigue, maintain a healthy body, and prevent various diseases. And it is
advisable for further research to find out the right and easy to do a type of physical
exercise.
Keywords: Anti-Fatique, Moderate intensity activity, Maximum heart rate
174
Neutrophil and Lymphocyte (N/L) Ratio of Gestation and Lactation
Sapera Goats at Kambing Farm, Sukoharjo, Ngaglik, Sleman
Anggitya Nareswari1 , Sugi Winarsih2 , Amelia Hana3 , Devita Anggraeni4 , Irkham Widiyono5 ,
Sarmin3
1
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
2
Food Agriculture and Fisheries Office of Sleman, DIY
3
Departement of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada
4
Departement Surgery, Faculty of Veterinary Medicine, Universitas Gadjah Mada
5
Departement Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada
ABSTRACT
The study aims to determine the ratio of neutrophil and lymphocytes (N/L) in the clinically
normal gestation and lactation Sapera goat. The samples were obtained from 12 Sapera goats
in three months gestation periode and 12 Sapera goats in three months lactation periode. Blood
samples taken via jugular vein are used for the preparation of blood smears to calculate the ratio
of neutrophil and lymphocytes. The average calculation results of the N/L ratio (p<0,05) in the
lactation group is higher (0,47 ± 0,23) than gestation group (0,19 ± 0,12) with a positive
moderate correlation (r = 0.59) between groups of gestation and lactation goats. The conclusion
of this study suggests that the average ratio of neutrophile and lymphocyte (N/L) of lactation
Sapera goats is higher than gestation Sapera goats, despite in normal range.
Keywords: Gestation, goat, lactation, ratio of N/L, sapera goat
175
Effect of PGF2α Dosage on Estrus Synchronization in Sows
MarchieAstridDaCosta1, and JonathanAnugrahLase2,
1Balai Pengkajian Teknologi Pertanian (BPTP) Maluku, Ambon, Indonesia

2Balai Pengkajian Teknologi Pertanian (BPTP) Maluku Utara, Sofifi, Indonesia
ABSTRACT
This study study aims to determine the effect of PGF2α dose on estrus synchronization
in sows. 10 sows aged 1-1.5 years with a body weight of 100-110 kg were used to test
the treatment of 5 mg PGF2α ((P1), 7.5 mg PGF2α (P2) and 10 mg (P3) doses.
Intramuscular (IM) and for the detection of estrus, 4 bulls aged 1.5-2 years were used.
The variables observed were estrus symptoms, time of onset of estrus, duration of
estrus and percentage of estrus. Data analysis used analysis of variance in a completely
randomized design with unidirectional patterns, while estrous symptoms were recorded
by recording each animal's behavior in an estrous state. The results of the analysis
showed that the PGF2α dose had no significant effect (P> 0.05) on the time of onset of
estrus, the length of estrus and the number of estrous animals, while the symptoms of
estrus shown by cattle in the three treatment factors were the same (namely:
restlessness, frequent urination. and always wants to get out of the cage). It was
concluded that for estrus synchronization in sows with a dose of 10 mg PGF2α was more
effective, while at a dose of 5 mg PGF2α was more beneficial.
.
Keywords: PGF2α, estrus synchronization, sows.
176
Ultrasonography in Leopard Gecko (Eublepharis macularius)
Febri Rizki Abdurahman1, Mokhamad Fakhrul Ulum2 , and Adi Winarto3
1Faculty
of Veterinary Medicine, IPB University, Bogor 16680, Indonesia
2Departement of Clicic, Reproduction and Phatology, Faculty of Veterinary Medicine,
IPB University, Bogor 16680, Indonesia
3Departement of Anatomy, Physiology and Pharmacology, Faculty of Veterinary
Medicine, IPB University, Bogor 16680, Indonesia
Abstract.
Ultrasonography is one of imaging procedures for examining organ and studying

anatomical structures that are non-invasive, painless, can be done quickly, and safe. This
research aims to study the anatomy of leopard gecko (Eublepharis macularius) internal
organs using ultrasonography method. Imaging ultrasonography Brightness mode and
color Doppler mode are performed on this study at trasnversal and longitudinal view
point using linear transducer in frequency of 15 MHz with the dorsoventral recumbency
position. The image results show an expressing the coccyx, fatty tissue and muscle tissue
at the normal tail, with cartilage cone in regenerated tail. The view point of abdomen
imaged can be identified the caudal part of lungs, liver, gall bladder, aorta abdominalis,
vertebrae, intestinal, follicles in the ovary (female), testicles (male), and body fat.
Ventricles of the heart observed in the thoraxic region. The internal organs in leopard
gecko expressed a different echogenicies, where are bones and lungs have hyperechoic
echogenicity. Liver, intestinal, follicles of the ovary, testicle, muscle, and body fat tend
to be hypoechoic with different levels of echogenicity. While lumen ventricles of the
heart , aorta , gall bladder, and urinary vesica have an anechoic image. The internal
organs of leopard gecko which large enough in size were nicely performed by
Ultrasonography in understanding an anatomy aspect.
Keywords: anatomy, leopard gecko, ultrasonography.
177
Effect of D-galactose on Weight Gain in Animal Model of Aging
Dwi Sunu Datrianto1, Yonathan Alvin Maruli Asi Sihotang2, Topas Wicaksono Priyo
Jr3, Surya Agus Prihatno3, Teguh Budipitojo4, Yosua Kristian Adi3*
1Graduate from Sains Veteriner Magister, Faculty of Veterinary Medicine, Universitas

Gadjah Mada, 55281 Yogyakarta, Indonesia
2Student from Sains Veteriner Magister, Faculty of Veterinary Medicine, Universitas
Gadjah Mada, 55281 Yogyakarta, Indonesia

Universitas Gadjah Mada, 55281 Yogyakarta, Indonesia

55281 Yogyakarta, Indonesia
ABSTRACT
D-galactose is widely used as an inducing reagent for animal models of aging. The aging
model by D-galactose induction has similarities with natural old. In this study, we
described the effect of D-galactose administration in rats against their body weight.
Thirty healthy male rats aged 2 months were divided into 2 groups. Control group was
treated with aqua sterile as placebo, while D-galactose group was treated with 3 mg/kg
BW of D-Galactose orally every day for 6 weeks. The rats in both groups were measured
using digital weigher every week. The data were analyzed descriptively for the average
of body weight (g) each week and statistically for the average of weight gain (%) after 6
weeks using t-test. The average of body weight in control group from week-0 until week-
6 were 119, 144, 160, 170, 179, 197, and 208, while in D-galactose group were 129, 151,
164, 173, 182, 192, and 197. Statistical analysis using t-test showed that there was a
significant difference of average weight gain in both groups (p<0.05). The average of
weight gain in control group and D-galactose group was 75.59%±21.19% and
53.76%±21.79%, respectively. D-galactose could decrease the weight gain in rat model
of aging.
Keywords: D-Galactose, Rats, Weight Gain, Animal Model, Aging.
178
Dermatophytosis in Cats: Clinical Signs and Identification of Etiological
Agent
Alsi Dara Paryuni1, Soedarmanto Indarjulianto2, Tri Untari3 and Sitarina Widyarini4*
1Doctoral
Student of Veterinary Science, Faculty of Veterinary Medicine, Universitas
Gadjah Mada, Yogyakarta
Mada, Yogyakarta
Mada, Yogyakarta
4Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta 55281
*Corresponding author: sitarina.widyarini@gmail.com
ABSTRACT
Dermatophytosis is a zoonotic disease caused by fungal infections of genus

dermatophyte. This disease is distributed globally and well concerned in public health.
This study aimed to investigate diagnosis and clinical signs of dermatophytosis in cats. A
total of 69 cats with one or more referable clinical signs of dermatophytosiswere
observed in this study. Physical examination, by using Wood’s lamp and microscopic
examination of hyphae (strip tape-test) on hairs, were used to preliminary screening of
dermatophytosis. Identification of fungi was conducted by scrapping area infection of
the skin and then cultured on Sabouraud dextrose agar. Results of this study
demonstrated that dermatophytosis in cat is characterized by the presence of multifocal
alopecia, scale, pruritus and erythema on the skin with a specific formation, known as
ringworm. Physical examination showed that 55 cats (79,7%) were positive fungal
infection by using direct Wood’s lamp examination and strip tape-test. Moreover,
identification of agent demonstrated that 58 (84%) and 11 (16%) of cats infected by
Microsporum canis and Trichophyton mentagrophytes, respectively. To conclude, cat’s
dermatophytosis caused by Microsporum canis and Trichophyton mentagrophytes with
typically lesion namely ring worm
Keywords: dermatophytosis, cat, clinical signs, etiological agent
179
Re-Epithelization and Density Of Collagen Fibers On Wound Healing Of
Mice’s Skin (Mus musculus) That Treated With Combination Of Chitosan
Membrane and Eel (Monopterus albus) Mucous
Deaoxi Renaschantika Djatumurti1, Afifatur Rafida1, Asha Yonika Putri Manalu2, Tri
Wahyu Pangestiningsih3*
1) Undergraduate student, Faculty of Veterinary Medicine, Universitas Gadjah Mada

2) Undergraduate student, Faculty of Medicine, Public Health and Nursing, Universitas
Gadjah Mada
3) Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada
*Corresponding author : estifkh@ugm.ac.id
Abstract
Tissue discontinuity in untreated skin wound can increase the incidence of infections that inhibit the
wound healing process. Chitosan and eel mucous have biodegradable, biocompatible, and
antimicrobial properties that potentially accelerate wound healing. This study aimed to determined
the potential of administration of a combination between chitosan membrane and eel mucous extract
in accelerating wound healing of skin in terms of re-epithelization and collagen fibers deposition.
Thirty adult male mice were divided into 5 groups, 6 animals/group. Skin wound were perform by
punch biopsy in 0.5 cm diameter in a sterile manner. Group I-IV respectively recieved: 10% povidone
iodine; eel mucus extract and gauze; combination of 1 % (w / v) chitosan membrane and eel mucus
extract; 1% (w / v) chitosan membrane. Group V (negative control) without therapy. Therapy was
given once a day for 10 days. At the 5th and 10th half of animals/group were euthanized and the wound
skin area were collected for histological preparation in hematoxylin-eosin and Mallory-azan staining.
Epithelial thickness and collagen density percentage were observed using light microscope and the
data were analyzed statistically using SPSS 16.The results showed a significant difference in epithelial
thickness on the 5th and 10th days (P 0.05) in combination group. The percentage of collagen density
at 10th day of combination group showed significantly difference (P 0.05) compared with control.
The conclusion is combination of 1 % (w / v) chitosan membrane and eel mucous extract has the most
potential to accelerate of re-epithelization and increase density of collagen fibers in the process of
skin wound healing.
Keywords: chitosan membrane; eel mucous; skin wound; wound healing
180
Detection of Ovine Herpesvirus-2 in Clinical Cases Of Sheep-Associated
Malignant Catarrhal Fever in Balinese Catlle and in Apparantly Healthy
Sheep In East Nusa Tenggara
Agus Wiyono1 , Harimurti Nuradji1, Maxs UE Sanam2, YohanesTRMR Simarmata2, and

Rini Damayanti1
1 Indonesian Research Center for Veterinary Sciences, Ministry of Agriculture, Bogor,
Indonesia.
2 Faculty of Veterinary Medicine, University of Nusa Cendana, Kupang, Indonesia.
ABSTRACT
Malignant catarrhal fever (MCF) is a disease causing a fatal outcome in cattle, and
generates economic loses worldwide. This study aims to detect the cause of the disease
in Balinese cattle showing clinical signs such as high fever, serous ocular mucopurulent
nasal discharges and enlargement of pre-scapularis and pre-femoralis lymphnodes.
These cattle were previously housed 50 meters away from a flock of sheep which were
brougt from Sabu Island 2 months earlier. Samples including blood, ocular, nasal and
vaginal swabs were collected from 22 sheep, 3 goats, 33 clinically healthy cattle (22
Balinese and 11 Ongole), and 3 infected Balinese cattle. Samples were processed and
tested using A nested polymerase chain reaction (PCR) test. Results showed that 12
sheeps out of 22 and 3 out of 3 infected Balinese cattle were positif MCF, suggesting a
potential spread of the disease from sheep to Balinese cattle. No goats and ongole cattle
which were positive indicates that these animals are less susceptible to Ovine
Herpesvirus-2 infection compared to Balinese cattle. The finding of 5 positive samples
from 22 healthy Balinese cattle shows the potential of sub-clinical infection of OHV-2.
Keywords: Ovine Herpesvirus-2, Malignant catarrhal fever, Balinese cattle, sheep and
PCR
181
Antibacterial Activity of Lactobacillus spp. Bacteriocin Against Resistant
Bacteria: A Review.
Allysa Shavira1 , Adi Imam Cahyadi2 , Sarasati Windria2,3
1Student of Veterinary Medicine Study Program, Faculty of Medicine, Universitas

Padjadjaran, Bandung, Indonesia
2Division of Microbiology, Department of Basic Medicine, Faculty of Medicine,
Universitas Padjadjaran, Bandung, Indonesia

3Division of Microbiology, Department of Basic Medicine, Veterinary Medicine Study
Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia Jl. Raya

Bandung Sumedang KM.21, Jatinangor, Hegarmanah, Sumedang, Jawa Barat, Indonesia
45363 Telepon 022-7796373, 7795594, Fax. 022-7795595
ABSTRACT
Antibiotic resistance is a worldwide problem that is often being studied and used as a
research topic because of the urgency based on the impact and linkages between cases
of resistance in humans and animals in a certain environment. Recent research related
to antibiotic resistance focuses on alternative uses of other substances or compounds
that have the same activity as antibiotics. Bacteriocins are antimicrobial peptides that
are ribosomally synthesized and able to kill or inhibit bacteria growth. Many bacteriocins
are produced by lactic acid bacteria, particularly from the genus Lactobacillus. This
review aimed to discuss the mechanism of the antibacterial activity of Lactobacillus spp.
bacteriocins against various bacteria, particularly resistant bacteria.
Keywords: antibacterial, bacteriocin, Lactobacillus, antibacterial mechanism, resistant

bacteria.
182
Study of calving interval (CI) and post partum estrus (EPP) in beef cattle
based on age, recording status and breed factors
Agung Budiyanto1*, Topas Wicaksono Priyo Jr1, Wisnu Krisna Nanditya2, Aghnia Afifia
Pranata3, Amrina Adila3, Arrum Tirtaningsari3, Azelia Astrid Surya Dewi3, Rifda
Nabila3
1Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Gadjah

Mada University, Indonesia
2Animal Health Center of Kalibawang Disctric, Kulon Progo Food and Agriculture Office,
Indonesia
3Undergraduate Veterinary Medicine, Faculty of Veterinary Medicine, Gadjah Mada
University, Indonesia
*Corresponding author: budiyanto@ugm.ac.id
ABSTRACT
A good reproductive performance will have an effect on increasing the efficiency of beef
cattle production. That factors including genetics and management. This study was to
determine the causes of CI and EPP its longer than normal. Its 205 beef cattle were used,
normal estrus, good health conditions and traditional management breeding systems.
Furthermore, it was divided into three research series, first based on breed, second on
age and third on recording status, the parameters data had taken were CI and EPP. The
research was conducted by observing and interviews with breeders and collecting data
from the farmer and conducted for 2 months. The results indicate that the age and breed
factors do not show a significant difference between groups, while the recording status
factor were showed a significant difference between complete data and incomplete
groups and no recording groups. From these results it can be seen that the management
factor affects the production performance of beef cattle compared to breed and age
factors as a genetic factors.
Keywords : CI, EPP, breed, age , management, beef cattle
183
Prevalence of Maedi-visna (Ovine Progressive Pneumonia) in Selected
Sheep Farms in Tarlac using Enzyme-Linked Immunosorbent Assay
(ELISA)
Gonzales, Maverick G.1*, Plaza, Lordy P.1,Francia, Allan Jeffrey E.2 and. Beltran, Ma.
Asuncion G.2
1 Veterinary Clinical Department, College of Veterinary Medicine, Tarlac Agricultural

University, Camiling, Tarlac, 2306, Philippines
2 Veterinary Clinical Department, College of Veterinary Medicine, Tarlac Agricultural
University, Camiling, Tarlac, 2306, Philippines

*Corresponding author: mulieri_lordy@yahoo.com
ABSTRACT
Maedi-Visna (Ovine Progressive Pneumonia), caused by Maedi Visna virus (MVV), is an

economically important viral disease of small ruminants particularly in imported sheep.
This study aimed to diagnose subclinical Maedi Visna cases using ELISA and to determine
the prevalence of MVV among sheep population in selected small ruminant farms in
Tarlac where farm owners are currently upgrading their stocks. Blood samples were
collected from 92 sheep from various sheep farms in the province. Blood serum was
harvested and was subjected for ELISA for the detection of antibodies for MVV. Initially,
there were samples that were deemed suspect of MVV. After two repetitions, the result
revealed that all the sheep were seronegative for MVV. In addition, risk factors
associated with the occurrence of the disease were checked: age, sex, source of origin,
and breed of the animal. The findings of the study affirmed that there are no existing
cases (zero prevalence) of MVV infection among sheep population in small ruminant
farms in Tarlac. This implies that the stocks in these farms are free of MVV. Still, there is
a need to institute strict control measures such as testing of imported sheep, and culling
positive animals or separation of infected animals for a continuous and effective
eradication of MV.
Keywords: Maedi-Visna, Ovine Progressive Pneumonia, Maedi-Visna virus, Sheep.

ELISA, prevalence
184
Level of Alanin Aminotransferase (ALT) and Aspartate Aminotransferase
(AST) Mus musculus Given a Compound of Temulawak, Turmeric, Kencur,
and Ginger
Anggi Muhtar Pratama1 , Agustina Dwi Wijayanti1, Dwi Cahyo Budi Setiawan1, Ida
Fitriana1, Aria Ika Septana1

ABSTRACT
Temulawak (Curcuma xanthorrhiza), Turmeric (Curcuma longa), Kencur (Kaempferia

galanga), and Ginger (Zingiber officinale) known as empon-empon which is the mainstay
herbal ingredients of society. Empirically, empon-empon is believed to cure various
types of diseases such as hypertension, rheumatism, diabetes mellitus, fever, and many
others. There is no information about the safety of consuming the ingredients empon-
empon on body condition, especially liver function. One indicator if there is liver
disorders that is an increase Alanin Aminotransferase (ALT) and Aspartat
Aminotransferase (AST) levels. Through this research it is expected to know the safety
of empon-empon consumption of liver function especially ALT and AST levels. In vivo
test used 15 male Mus musculus with a body weight of 20-25 grams and 2 months of
age. Experimental animals adapted for 7 days, then were divided into 5 groups, negative
controls (K-) given aquades, positive control (K+) given stimuno® dosage as
recommended by the label, treatment group 1 (P1) treated dosage 250 mg/kg body
weight, treatment group 2 (P2) treated dosage 500 mg/kg body weight, treatment group
3 (P3) treated dosage 1000 mg/kg body weight. The compound is given every day once
with intragastric sonde. On the 7th day after treatment, plasma was taken through a
lateral vein (tail) to observe ALT and AST levels. The results showed that there was an
increase ALT level in the P3 group; whereas AST levels increased in the K-, K+, P1, and
P3 group (P2 group is significantly different from the K+, P1, and P3 group (p ≤ 0.05)).
The concluded that the dosage of 500 mg/kg body weight does not cause changes in ALT
and AST levels. Nevertheless, various studies still need to be carried out to ensure that
a dosage of 500 mg/kg body weight is a safe dose for consuming the compound of
temulawak, turmeric, kencur, and ginger every day.
Keywords: ALT; AST; Mus musculus; Compound of temulawak; Turmeric; Kencur; Ginger
185
Comparison of growth, scrotal biometry, libido status and semen quality
of Malaysian Kedah-Kelantan (KK) and KK ×Brangus bulls at puberty
Mohammed Sirajul Islam1,2. Nurhusien Yimer Degu*1. Abd Wahid Haron1. Faez
Firdaus Jesse Bin Abdullah1. Mark Hiew Wen Han1. Mamat Hamidi Kamalludin3,
Obedullah Kaka4 and Wan-Nur Fitri1
1Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti

Putra Malaysia, 43400 Serdang, Selangor, Malaysia, 2Bangladesh Livestock Research
Institute, Savar, Dhaka-1341, Bangladesh. 3Department of Animal Science, Faculty of
Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
4Department of Companion Animal Medicine and Surgery, Universiti Putra Malaysia,
43400 Serdang, Selangor, Malaysia
ABSTRACT
A total of 10 experimental bulls consisting Malaysian Kedah-Kelantan (KK) breed and KK

× Brangus bulls were studied to compare their growth performance, scrotal biometry,
libido status, testosterone concentration, and semen quality at puberty. Body weight
and reproductive measurement, blood and semen sampling were accomplished once a
month from April 2019 to September/2020. Results showed that mean age at puberty
were 18.00 and 23.50 months for KK and KK× Brangus bulls, respectively with
significantly (p˂0.001) different. Conversely, average body weight (kg) and average daily
gain (kg/day) were significantly larger (p˂0.001) in KK × Brangus bulls than KK bulls.
Significantly (p˂0.05) better libido score and higher level of testosterone at age in
puberty were found in KK bulls compared to KK × Brangus bulls. Moreover, progressive
motility, sperm concentrations and total sperm cells /ejaculation was significantly
(p˂0.05) more in KK rather than KK × Brangus bulls. Furthermore, a positive correlation
was found among blood serum testosterone, body weight and scrotal circumference in
both breed types of bulls. In inclusion, the reproductive performance of KK bulls are
superior in terms of libido and semen production and quality with earlier age of puberty
compared to KK × Brangus which clearly indicate the breeding potentiality of Kedah-
Kelantan bulls.
Keywords: Kedah-Kelantan, puberty, growth, libido, and semen quality.
186
Effect of Storage Time and Temperature on Haematological Parameters
of Dogs using The VetScan HM5 Analyzer
Dorothea Vera Megarani1 Dinar Arifianto1, Christin Marganingsih Santosa1, Siti Isrina
Oktavia. Salasia1, Adecitra Mutiarinda2, Rosa Yaneswara Murti Pujaningrum2, and
Yudith Violetta Pamulang2
1Departement of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah

2Veterinary Student, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
ABSTRACT
Haematological analyses are usually performed immediately after sampling or within 24

hours. However, in most cases, the delay up to 48 hours may occur. This study was done
to measure and determine the stability and validity of the haematology results of the
blood samples stored at 4°C and room temperature for 24 and 48 hours using VetScan
HM5. One ml blood from 16 physically healthy female mix-breed dogs was collected and
put into two EDTA K-3 tubes (0.5 ml each), then stored at 4°C and room temperature.
The haematological analysis was performed at three-time points using the VetScan
HM5, T0 (within 1 hour), T24, then T48 for each sample stored at both temperatures. All
haematological parameters were analysed using Two-Way Repeated Measure Analysis
of Variance (Two-Way RM-ANOVA). This study reported that most of haematological
parameters were unstable, and other parameters that did not change clinically had
diverse results after 24 and 48 hours of storage at both temperatures. This indicated
that the blood storage was associated with changes in blood cell artifacts, so that the
blood samples should be processed and analysed in less than 24 hours using VetScan
HM 5 for accurate and valid interpretation.
Keywords: blood storage, haematology, clinical pathology, dog, VetScan HM5
187
Diagnosis of Mycoplasma Infection Using ImmunoComb Assay in Free
Range Chicken
Paragas, Annalie B1, and Beltran, Ma. Asuncion G.2

1,2College of Veterinary Medicine, Tarlac Agricultural University, Philippines
ABSTRACT
This study was conducted to detect the antibody titer of specifically, Mycoplasma
gallisepticum and M. synoviae on the free-range chicken project at the Tarlac
Agricultural University and its beneficiaries in Sta Ignacia. A total of 110 blood samples
of free-range chickens were collected, regardless of age, sex and breed. These samples
were tested for Mycoplasma infection using the ImmunoComb ® Assay. Results showed
the 56.4% were found positive of Mycoplasma synoviae and 46.3% positive of
Mycoplasma gallisepticum. However, even they were found positive, the antibody titers
for Mycoplasma synoviae was only 1.82 ImmunoComb® unit in Tarlac Agricultural
University – FRC Project and 1N.34 ImmunoComb® unit in Sta Ignacia FRC beneficiaries
which mean weak positive, and Mycoplasma gallisepticum with 1.40 ImmunoComb®
unit in Tarlac Agricultural University – FRC Project and 1.11 ImmunoComb® unit in Sta
Ignacia FRC beneficiaries also were found to be weak positive.
Keywords: ImmunoComb®, Mycoplasma gallisepticum, Mycoplasma synoviae,
188
Efficiency of Different Natural Fibers as an Alternative Non-Absorbable
Surgical Suture Material in Rabbit (Oryctolagus cuniculus)
Krestel Joy Isla1.* , Angelie Cudia1 , Lavina Gracia Ramirez1 and Ma. Asuncion Beltran1
1College of Veterinary Medicine, Tarlac Agricultural University, Tarlac, Philippines 2306

*Corresponding author: kresteljoy_isla@yahoo.com
ABSTRACT
This study was conducted to determine the efficiency of different natural fibers as an
alternative non-absorbable surgical material in rabbits. Specifically, be able to present a
medical use of banana, jute, and snake plant in surgical operations by producing a non-
absorbable multifilament surgical suture thread, to compare with the desirable
properties of a surgical suture, and assess if the produced surgical suture from different
natural fibers would elicit an irritating effect. Thirty-six (36) albino rabbits, regardless of
sex, ages seven months, were randomly grouped into four treatments, replicated thrice
with three rabbits per replication following the Complete Randomized Design.
Treatment 1 was sutured with commercial silk, treatment 2 was sutured with jute fiber,
treatment 3 was sutured with banana fiber, and treatment 4 was sutured with snake
plat fiber. Observation of gross lesion was done on day 7 and 14 post-treatment.
Histopathology was done on the 7th day of post-treatment. The study revealed that all
the treatments were not significantly different from each other based on the gross
lesions. However, snake plant fiber shows delayed wound healing process based on the
histopathological result. Hence, jute fiber and banana fiber are efficient natural fiber as
an alternative non-absorbable surgical material in rabbits.
Keywords: rabbit, surgical suture, banana, jute, snake plant
189
Bovine Umbilical Vein Endothelial Cells Conditioned Medium Prevent
Neuronal Cell Apoptotic on The In Vitro Model Neurodegeneration
Gregorius Viktor Lembang1, Vinny Anisya Larasati1, Hevi Wihadmadyatami1, Dwi Aris
Agung Nugrahaningsih2 and Dwi Liliek Kusindarta1*

Yogyakarta, 55281
2Department of Pharmacology, Faculty of Medicine, Universitas Gadjah Mada,
*Corresponding author: indarta@ugm.ac.id.
ABSTRACT
Neurodegenerative disease is a disease that results from progressive degeneration

and/or death of neuron cells. Recently, mesenchymal stem cells (MSCs) were used as a
therapeutic agent in various diseases. The MSCs have some disadvantages, such as the
need for research about the therapeutic mechanism, immunotolerant, tumorigenesis
reaction, and transplant rejection. The use of conditioned media as a cell-free therapy
has a large prospect because it takes the minimum risk and allogeneic transplant
possibility. Recently the MSCs and their exosomes derived from humans, indeed the
large issued mainly in the Muslim country, are the use of human product do to the
"halal" term of the product. The purpose of this research is to find out the ability of
Bovine Umbilical Vein Endothelial Cell Conditioned Medium (BUVEC-CM) as a candidate
of neuroprotectant on the invitro model neurodegenerative. SH-SY5Y were cultured in
the presence of BUVEC-CM, TMT, and Donepizil HCl. In addition, we do perform the
HRMS and in-silico modeling. Our results found the BUVEC-CM able to prevent neuronal
apoptotic mediated by inhibition of the Caspase pathway.
Keywords: Apoptotic, BUVEC-CM, Neurodegeneration, SH-SY5Y
190
Investigation of Chlamydophilosis from naturally infected cats
Madarina Wasissa1, Fajar Budi Lestari2,3, Alfarisa Nururrozi4, Ida Tjahajati4,

Soedarmanto Indarjulianto4, Siti Isrina Oktavia Salasia1*
1
Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
2
Department of Bioresources Technology and Veterinary, Vocational College, Universitas
3
Inter-Department of Biomedical Sciences, Faculty of Graduate School, Chulalongkorn
University, Bangkok, Thailand
4
Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
ABSTRACT
Background and aim: Chlamydophila felis formerly known as Chlamydia psittaci var. felis is
frequently associated to ocular, respiratory, and occasionally reproduction tract infection. Even
though the infection is sometimes asymptomatic, it can cause immunosuppressive latent
infection. Cp. felis is sometimes associated to co-infection in viral disease. This paper work is of
interest and curiosity to gain the broader knowledge about Cp. felis as frequent infectious agent
found in cats during the investigation of microbial agent associated to infectious viral infection
in our laboratory. Moreover, related to the public health issue, this agent may potentially cause
zoonotic disease.
Methods: The observation was conducted in three cats with clinical signs of Cp. felis infection,
particularly with relapsing conjunctivitis. The cats’ history backgrounds were recorded based on
the owner information. Conjunctiva scrapping was sampled for cytology examination and
molecular assay detection using polymerase chain reaction (PCR) targeting the outer membrane
protein gene in 277-base pair. Phylogenetic tree was generated using MEGA-X software to show
the agent group cluster. Post-mortem examination was performed in one cat which was died
during the examination. Histopathology examination was conducted respectively under the
owner consent.
Results: Basophilic inclusion within conjunctiva epithelial cell was found during the cytology
examination in acute infection. However, the inclusion was not found in cat with chronic fatal
infection. Three sequenced isolates demonstrated in the same main cluster Cp. felis isolated
from the other countries. Fatal chronic infection demonstrated marked purulent inflammation
in lungs and heart. The incidence of the chronic fatal case presumably was caused by the other
co-infections. However, in this case viral co-infection was not able to be proven.
Conclusion: Molecular assay of Cp. felis is always recommended to obtain the definitive
diagnosis of chlamydophilosis particularly in relapsing conjunctivitis cat patient. Even though it
is not often fatal, the infected cat will be a carrier which possibly spread the pathogen in the
surround environment.
Keywords: Cat, Chlamydophilosis, Chlamydophila felis, co-infection, conjunctivitis,

histopathology, zoonotic.
191
Application of Blood Cockle (Anadara Granosa) Shells Paste on Rat Bone
Fracture
Asfarina, Z.M.R, Dwita Husaeni, and Devita Anggraeni*

*Email : devita_vet@ugm.ac.id
ABSTRACT
Bone fracture is a break in the continuity of bone which followed by injury of the
surrounding tissues. Calcium is known as an essential substance for maintaining bone
health. Blood cockle (Anadara granosa) shells have high calcium levels that potentially
used as alternative source of calcium. The aim of this study was to study the effect of
blood cockle (Anadara granosa) shells paste on rat bone fracture histopatologically.
Fifteen male Wistar rats at 3 months of age were used in this study. After a week of
adaptation, rats were randomly divided into 3 groups of 5. All rats were undergone
sugery for making transverse fracture on the left femur and then followed by fixation
using stainless steel intramedullary pin. Group I was control group, meanwhile, group II
and III were given mixed of blood cockle shells powder and sodium alginate in 1:0.5 and
1:1 ratio, respectively, at fracture area. After 4 weeks of treatment, all rats were killed
and femur were taken out. Femur was fixed in 10% formalin solution for
histopathological examination using Hematoxylin Eosin (HE) staining. The results
showed that there was domination of fibrous connective tissue in the fracture area of
control group. Meanwhile, cartilage and trabecular bone was shown in group II and III,
however, development of mineralized cartilage and trabecular bone more dominant in
Group III. In conclusion, application of mixed blood cockle shells powder and sodium
alginate in 1 : 1 ratio was ameliorate fracture area histopathologically.
Keywords: blood cockle shell, fracture, rats
192
The Effectiveness of Commercial Bleach And Disinfectants on Bacillus
Antrachis Spore
Rahmat Setya Adji1.* , and Harimurti Nuradji1
1 Indonesian Research Center for Veterinary Sciences, Ministry of Agriculture, Bogor,

Indonesia.
* Corresponding author: rs.adji@yahoo.com
ABSTRACT
Antraxs is a zoonotic disease caused by Bacillus antrachis. This disease has been
reported to cause the disease in human and animal in Indonesia. Bacillus antrachis spore
is reported to remain viable for around 250 years. One of the difficulties to prevent the
disease is because of the present of the spore in the environment. With the improper
disinfection, Bacillus antrachis spore can survive and infect animal and human. This
study aims to evaluate the use of commercial bleach to inactivate the spore of Bacillus
anthracis. Suspension containing Bacillus anthracis spore was exposed to commecial
bleach, iodine and hydrogen peroxyde with concentration of 0.5%, 1%, 2% and 3% for 1,
2 , 4 and 6 hours. Formalin with the concentration of 10% was used as control. Results
shows that all concentration of commercial bleach (sodium hypochlorite, NaClO), Iodine
(iodophore) was able to inactivate the spore for 1,2, 4, and 6 hours. This effectivness
was comparable to Formalin 10%. Hydrogen peroxyde was able to inactivate the spore
at 1 hour with the concentration of 4%. This finding indicates that commercial bleach,
iodine, and hydrogen peroxyde can be used for disinfection for Bacillus antrachis spore
with the proper concentration and contact time.
Keywords: Commecial bleach, iodine, hydrogen peroxyde, spore, Bacillus antrachis
193
First Report for Isolation and Identification of Avirulent Strain of Bacillus
anthracis from Environmental Samples in Central Java, Indonesia
1,2 1 2 2*
Ully Apriliana , Hendra Wibawa , Tri Untari , and Soedarmanto Indarjulianto
1BalaiBesar Veteriner Wates Yogyakarta, Jalan Raya Jogja-Wates Km 27 Po Box 18,

Wates, Yogyakarta, 55602, Indonesia
2Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang,
Yogyakarta, 55281, Indonesia

ABSTRACT
This study aimed to detect and characterize Bacillus anthracis found on soil and straw in
endemic areas. An avirulent vaccine of B. antrachis (strain 34F2) were used as a control,
while the soil samples were collected from Gunungkidul, Yogyakarta (n=2) and Boyolali,
Central Java (n=1), and the straw sample was obtained from Pati, Central Java (n=1).
Isolation and identification were performed using a conventional method: morphology
of bacteria colony in solid media, Gram staining, capsule staining, spores staining, and
the motility test. Isolates were then identified using polymerase chain reaction (PCR)
against Ba813, lef (pXO1), and capC (pXO2) gene. All samples grew on blood agar with
ground-glass appearance, white-grey colony (Gunungkidul and avirulen strain) or
yellowish (Boyolali and Pati), square-ended rods, Gram+, long chain formation, had
spore, then identified as B. anthracis. Boyolali, Pati and avirulent strain isolates had
slightly different characteristics including grew non-mucoid in the bicarbonate agar
medium, and uncapsulated. The PCR showed two Gunungkidul isolates amplified 3 gene
including Ba813, lef, and capC gene, however the other isolates not amplified capC gene.
Concluded that two Gunungkidul isolates were identified as virulent strain of Bacillus
anthracis, while the Boyolali and Pati isolates proposed as avirulent strains, that first
finding in Central Java.
Keywords: avirulent Bacillus anthracis, Boyolali, identification, Pati, PCR multiplex.
194
The Distribution of Steroid Sex Hormones in Sunda Porcupine (Hystrix
javanica) Adrenal Gland
Budipitojo Teguh1*, Masithoh Dian Bekti Hadi2, Padeta Irma1 and Budiariati Vista1
1Department of Anatomy, Faculty of Veterinary Medicine Universitas Gadjah Mada,

2Student of Doctoral Program Veterinary Science, Faculty of Veterinary Medicine
Universitas Gadjah Mada, Yogyakarta, Indonesia

*Corresponding author: budipitojo@ugm.ac.id
ABSTRACT
The Sunda porcupine (Hystrix javanica) is an endemic species of Indonesia. This animal
can be found in Java, Bali, Sumbawa, Madura, South Sulawesi, and Lombok. Sunda
porcupine have high endurance because they can survive in any kind of weather.
Utilization of porcupines is increasing in Indonesia so it is necessary to do captive
breeding so that porcupine populations can be maintained and their use preserved. The
adrenal glands produce various hormones such as steroid sex hormone. This study
determined the present of steroid sex hormones in the sunda porcupine adrenal glands
and its distribution. Adrenal gland samples were taken from nine Javan sunda
porcupines from Tawangmangu. The hormones in the adrenal glands were visualized by
immunohistochemical (IHC) staining method. The results showed that the testosterone
and estrogen receptor immunoreactive cells were found in the zona reticularis of the
cortex adrenal of Hystrix javanica.
Keywords: Adrenal gland, Estrogen receptor, Hematoxilin Eosin, Sunda Porcupine,

Testosterone
195
Duration of Imunity Against Leptospirosis and Rabies Commercial
Vaccination in Working Dogs
Ambar Retnowati1, Agustin Indrawati2, Upik Kesumawati Hadi2, Safika2, Susan

M.Noor3
1Doctoral
Student of Specialization Medical Microbiology , Health Faculty Veterinary

3Indonesian Research Centre for Veterinary Science,Bogor
ABSTRACT
Working dogs are dogs that interact very closely with humans so that it is feared that disease
transmission from animals or humans (zoonosis) will occur. Zoonotic diseases that get
attention are Rabies or Leptospirosis. As one of the precautions taken for these working dogs
is a vaccination program. This study aims to determine the level of protection of dogs against
the commercial combo rabies vaccine containing a combination of rabies leptospirosis,
distemper, hepatitis, parvovirus and parainfluenza carried out in the K9 working dog unit of
the Directorate of Customs and Excise, Ministry of Finance, Lido. The research was conducted
on 10 working dogs more than 1 year old. The examination included observation of post-
vaccination symptoms using serum samples taken after one month, 4 months and 7 months
of vaccination against rabies and leptospirosis. The results of Leptospirosis serological
examination using the Microscopic Agglutination Test (MAT) method showed that the
presence of agglutination on ichterohaemorragie serovars with antibody titers equal to1:100
or more in the first month with a protection of 70% while the protection was 30% at the
fourth month and 20% at month 1. The seventh month with a titer of 1:100. All samples did
not show agglutination against serovar canicola at 7 months post vaccination but at 1 and 4
months post vaccination showed 10% protective results. In this study also found a cross
reaction against non-vaccine serovars, namely serovar celledoni and javanica. The test results
using Enzyme Linked Immunosorbent Assay (ELISA) against rabies in this study showed that
dogs vaccinated after one month provided 100%, 90% protection for 4 months after
vaccination and decreased to 20% after 7 months. This study illustrates that the study of the
duration of immunity to the combo Rabies vaccine in the field is still above the vaccination
coverage in at-risk dog populations and endemic areas. The emergence of other serovars on
leptospirosis agent examination is due to cross-reactions and it is necessary to watch out for
whether there is an increase in titer against non-vaccine serovars
Keywords: Immunity, Leptospirosis, Rabies, Vaccination, Dogs
196
Molecular characterization and phylogenetic analysis of TevAT1 gene in
Trypanosoma evansi isolated from buffaloes in Ngawi district, Indonesia
Zs Ahmad Fauzi1 , Nuryady Moh Mirza2,3, Wusahaningtyas Lu’lu’ Sahara2 , Nurcahyo R.

Wisnu2
1Tropical Medicine Department, Faculty of Medicine Public Health and Nursing,
2Sains Veteriner Program, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Indonesia
3Biology Education Department, Faculty of Teacher Training and Educationn,
Universitas Muhammadiyah Malang, Indonesia
ABSTRACT
Resistance to the diminazene aceturate (DA) drug was a major problem to control
Trypanosoma evansi . Trypanocidal drug resistance was currently reported in several
regions in Indonesia. Resistance was the result of DA uptake that was lacking by
parasites. Reduction of DA uptake due to changes in the TevAT1 gene encoding
adenosine transporters 1. However, there was no genetic information available about
the TevAT1 gene, so that rapid detection with molecular for DA drug resistance had
never been conducted. Therefore, it was necessary to conduct research in gene mapping
on T. evansi that played a role in drug resistance TevAT1 gene. The genomic DNA was
isolated from T. evansi that infected buffaloes in Ngawi, Indonesia. Polymerase chain
reaction (PCR) was carried out to amplify TevAT1 gene using the designed primers. The
PCR product was cloned into pTA2 plasmid vector and transformed into Escherichia coli
DH5α cells. The sequence was analyzed and compared to the Gene bank. BLAST results
showed that the TevAT1 gene from Indonesia had a high level of similarity (99%) to all
Adenosine Transporter 1 genes in Gene Bank. Furthermore, alignment results with T.
brucei (TbAT1) gene TbAT1r (AF152370) presented 14 points of nucleotide base
differences and nine differences at amino acid level.
Keywords: Phylogenetic analysis, Trypanosoma evansi, diminazene aceturate, TevAT1
197
Molecular Identification of Trypanosoma sp. Derived from Cat in
Yogyakarta Using The Internal Transcriber Spacer-1 (ITS-1)
Dwi Priyowidodo1, Ana Sahara1, Joko Prastowo1, Wisnu Nurcahyo1, and Lintang
Winantya Firdausy2,

Jl. Fauna No. 2, Karangmalang, Yogyakarta, 55281, Indonesia.
2Veterinary Science Program, Faculty of Veterinary Medicine, Universitas Gadjah
Mada, Jl. Fauna No. 2, Karangmalang, Yogyakarta, 55281, Indonesia.
ABSTRACT
The occurrence of trypanosomiasis which happened naturally in domestic cat is very

rare. There was no report about the species of Trypanosoma that infecting cats naturally
in Indonesia. Thus this study was aimed to know the species of Trypanosoma that have
infected a domestic cat in Yogyakarta, molecularly using ITS-1 with Trypanosoma evansi
isolate from buffalo as a comparison and also to determine the nucleotide variation
effect on host transfer. Blood was collected from four different animals, first sample was
taken from a cat that had been diagnosed with Trypanosoma from blood smear, second
sample came from mice which infected with Trypanosoma from the cat, and the others
came from mice that had been inoculated with Trypanosoma evansi isolate from
buffalo. All samples were taken when the peak of parasitemia was reached and the DNA
was isolated further. Amplification was carried out using ITS-1 Trypanosoma primer then
sequenced and analyzed using MEGA X. The result showed that all of samples were
identic with Trypanosoma evansi, including the cat’s sample. Each sample has an
amplification product around 410 bp with several nucleotides variation. Those
differences indicate that the host’s transfer, either intra species or inter species, took
the effect of variation.
Keywords: cat, host ITS-1, molecular identification, trypanosomiasis
198
Detection of Spirometra spp. in Edible Frogs Sold at Camiling, Mayantoc,
and Santa Ignacia Public Markets
Regina Villarama1, Joanna Rose Pulmano1, Krestel Joy Isla1 , and Allan Jeffrey Francia1
1College of Veterinary Medicine, Tarlac Agricultural University, Philippines 2306
ABSTRACT
The study was conducted to detect and to identify the prevalence rate of Spirometra
spp. on 120 Philippine edible frogs sold at Camiling, Mayantoc, and Santa Ignacia Public
Markets. A total of 40 frog samples in each public market were collected regardless of
sex, age and weight. For the examination for the presence of Spirometra spp. (spargana,
larval form) leg muscles of the frogs were incised and examined. Frog samples from
Camiling Public market, 11 frogs or 27.50% was detected, 13 frogs were positive or
32.50% from Mayantoc, and 8 frogs or 20% from Santa Igancia public market. An
Information Education and Communication (IEC) materials was developed for public
health awareness about Spirometra ssp. on edible frogs
Keywords: edible frog, spargana, tapeworm
199
Characterization Of Gen Coding Fusion Of Newcastle Disease Virus
Infected Natif Chicken (Gallus gallus domesticus) in Surabaya
Maha kirana1, Fedik Abdul Rantam2 , Rahaju Ernawati2 , and Jola Rahmahani2
1Magister Program in vaccinologya & imunoterapetika, Faculty of Veterinary

Medecine, Airlangga University, 60115 Surabaya, Indonesia
2Depart of Microbiology, Airlangga Universty, 60115 Surabaya, Indonesia
ABSTRACT
Newcastle Disease was one of the important disease in the poultry industry, because it
was contagious, and had the potential to cause economic losses.This study was aimed
to determine the molecular characteristics of the virus based on the homology,
evolutionary distance, phylogenetic of the Fusion protein Newcastle diesease virus in
native chickens in Surabaya using several comparisons of isolates from GenBank.
Research samples were scour of digestive organs from domestic chicken, that were
collected from traditional market in Wonokromo, Surabaya. Samples were tested using
RT-PCR with primer forward and reverse with target 976bp, positive sample which is
continued with sequencing then homology, evolutionary distance and phylogenetic
tree. The results of the chc / SBY / 2018 homology sample have 88% similarity with the
reference sequence, and the highest homology are shown by comparison with 90-95%
of various isolates from Indonesia. The greatest evolutionary distance in the comparison
of Chc / SBY1 / 2018 isolates compared to Lasota vaccine isolates was 8.1%, and the
evolutionary distance between samples and virus isolates circulating in various parts of
Indonesia ranged from 3.0% to 5.7%. And the results of the phylogenetic , chc / SBY /
2018 still one clade with the comparison isolates.
Keywords: Newcastle Disease, Homology,filogenetic
200
Survey of Pet Owners’ Knowledge, Attitude and Perception on
Antimicrobial Resistance and Compliance to Antibiotic Prescription in
West Coast Division of Sabah, Malaysia
Adellie Dompok1 , Arifah Abdul Kadir2, *, and Shanmugavelu M Sithambaram1
1Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
* Corresponding author: arifah@upm.edu.my
ABSTRACT
Antimicrobial use in companion animals has been scrutinized due to the emergence of
antimicrobial resistant (AMR) pathogens in bacterial isolates. This study aims to
investigate pet owners’ knowledge, attitude and perception (KAP) towards AMR and
compliance of antibiotic prescription. An online questionnaire was distributed to 210
pet owners in the West Coast Division of Sabah via social media over a period of 3 weeks.
Descriptive statistics were computed and data was presented as frequencies and
percentages. The KAP sections were further subjected to reliability testing and scoring.
Inferential statistics was used to compare the median total score of KAP to selected
demographic profile. Despite the pet owners having moderate knowledge (65%),
excellent attitude (100%) and good perception (86%) towards antibiotic usage for their
pets, it was discouraging to note that 63% claimed that they were not aware of the
occurrence of AMR in pets. A majority of the respondents (96%) wished that the
veterinarian had provided more detailed information on AMR before prescribing
antibiotics for pets. In conclusion, veterinarian-pet owner interactions should be
ameliorated in order to augment antimicrobial stewardship and hence, reduce the
occurrence of AMR.
Keywords: pet owners, questionnaire, companion animal, antimicrobial use,

antimicrobial resistance.
201
Subchronic Toxicity of New Medicine Candidate (+)-2,2’-Epicytoskyrin A
for Mice
Ni Luh Putu Rischa Phadmacanty1, Dewi Wulansari2, Andria Agusta2 , Sitarina

Widyarini3, and Kurniasih3

Sciences, Bogor, Indonesia
2Department of Botany, Research Center for Biology, Indonesian Institute of Sciences,
Bogor, Indonesia
3Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta Indonesia
ABSTRACT
(+)-2,2'- Epicytoskyrin A is a secondary metabolite from Diaporthe sp. This component

can be a new medicine candidate because of its potency as an anticancer and an
antibiotic. Many data about the potency of (+)-2,2'- Epicytoskyrin A, but limited data
about the negative effect. This study aimed to study the subchronic toxicity of this
component in mice. This study used 60 male mice (Mus musculus) which were divided
into four group including control (K) 1 % CMC Na, P1 (+)-2,2'-Epicytoskyrin A dosage 6,25
mg/kg BW in 1 % CMC Na, P2 (+)-2,2'-Epicytoskyrin A dosage 12,5 mg/kg BW in 1 % CMC
Na, P3 (+)-2,2'-- Epicytoskyrin A dosage 25 mg kg BW in 1 % CMC Na. Parameters that
were analyzed were body weight, food consumption, blood chemicals, and histology.
The results showed no significant difference in body weight gain and food consumption
in all groups. Blood chemicals analysis showed increasing AST and ALT in dosage 12.5
mg/kg BW groups at day 90. Histological changes showed fatty degeneration and
hydropic degeneration in the liver and mild inflammation in the kidney. No irreversible
toxic effect of this component. So it can be beneficial for plant-based drug discovery.
Particularly this study provides information about the safety of (+)-2,2'-Epicytoskyrin A
as a medicine candidate
Keywords: subchronic, toxicity, (+)-2,2’- Epicytoskyrin A, mice
202
The Effect of Heat and Disinfectants on Infectious Bursal Disease Virus
Harimurti Nuradji1 and Qadrina Ayu Besticia1
1Indonesian Research Center for Veterinary Science, Bogor
ABSTRACT
Infectious Bursal Disease (IBD) has been reported in Indonesia since 1983 and become
an endemic disease. The IBD virus is known to be quite resistant to physical and chemical
reagents compared to other viruses, causing this disease is hard to be eradicated. This
study aims to evaluate the effect of heat and disinfectants on the viability of IBD virus.
This study was conducted using local isolate of IBDV obtained from IRCVS. The virus was
exposed to heat and disinfectant. Heat treatment was conducted by exposing the virus
with 560C and 600C for 30 minutes, 1, 2 and 5 hours. Similarly, virus was also treated
with two disinfectants, virkon and sodium hypochloride (bleach) for 30 minutes, 1, 2 and
5 hours with different concentrations. Results showed that virus can be inactivated at
temperature of 800C and 560C for 2 and 5 hours respectively. Virkon at concentration of
1:200 and 1:400, was able to inactivate the virus at 30 minutes, 1, 2 and 5 hours, while
sodium hypochloride 0.5% requires at least 60 minutes to inactivate the virus.
Keywords: Infectious bursal disease, heat, chemical disinfectants
203
The Effect Of Commercial Detergents On Newcastle Disease Virus
Harimurti Nuradji1 and Ghea Aquatica1
1 Indonesian Research Center for Veterinary Sciences, Ministry of Agriculture Indonsia.
ABSTRACT
Newcastle disease is considered to be one of the most important poultry viral diseases
due to its ability to cause huge economic losses with up to 100 percent mortality in
chicken. A number of disinfectants have been used to prevent the diseases in the farm.
However, the use of existing commercial disinfectants nowadays is considered
expensive. This study aims to evaluate the effectiveness of commercial detergents as a
disinfectant to inactivate the virus. Two commercial detergents containing Alkylbenzene
sulfonates which can dissolve the lipid of the viral envelope were evaluated. Three
concentrations of 1%, 1.5%, and 2% were used, with contact time of 5, 10, and 15
minutes. Virkon, the commercial disinfectant, was used as control. The results showed
that commercial detergents had the same results compared to control by being able to
inactivate the virus with a concentration of 1%, 1.5% and 2% in 5, 10 and 15 minutes.
This suggest that household detergents which are easy and affordable can be used as an
alternative for disinfection.
Keywords: Newcastle disease, Disinfectant, Commercial Detergent,
204
Case Report: Isosporiasis in a Domestic Cat
DodikPrasetyo1* AuliaMaurenda2, NurfitriyanaFirsty3, TheresaPramesti4 and

AisyaInayatillah5
1Animal Clinic Department, Faculty of Veterinary Medicine, Brawijaya University

2,3,4,5 Veterinary Student, Faculty of Veterinary Medicine, Brawijaya University
*Corresponding author: dodik_prasetyo@ub.ac.id
ABSTRACT
Isosporiasis is a digestive tract disease caused by Isospora felis. A stray female domestic
cat was found with diarrhea, anorexia, lethargy, and the presence of ectoparasite. The
supporting diagnoses that are carried out are hematological examination and native
fecal examination. The results of the hematological examination showed leukocytosis,
lymphocytosis, thrombocytopenia and granulocytosis, while the native fecal
examination showed the presence of Isospora felis oocysts. The therapy was given by
giving metronidazole 50 mg/kg BW once a day, probiotic once a day, and fluid therapy
for 7 days. The therapeutic response shows improvement marked by the absence of
oocysts in the feces after one week of therapy
Keywords: Domestic Cat, Isospora felis, Isosporiasis, Metronidazole
205
Mucin heterogeneity in biliary tract of hamster and mouse models for
Opisthorchis viverrini infection
Woro Danur Wendo,1,3* Theerayut Thongrin,1,4 Sutas Suttiprapa,2,3 Prasert Saichua,2,3

Prasarn Tangkawattana,1 Sirikachorn Tangkawattana1,3**
1Facultyof Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
2Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
3Tropical Disease Research Laboratory, Faculty of Medicine, Khon Kaen University,
Khon Kaen 40002, Thailand

4Small Animal Hospital, Faculty of Veterinary Medicine, Western University,
Kanchanaburi 71170, Thailand

*Presenter **Corresponding e-mail: sirikach@kku.ac.th
ABSTRACT
The carcinogenic liver fluke (Opisthorchis viverrini, Ov) could survive in the bile duct of
the golden Syrian hamster, but was expelled from the biliary tract of the BALB/c mouse
at the early stage of infection. Timely expression of mucins with existence of goblet cell
metaplasia is believed to be the crucial response of the host biliary epithelium upon the
presence of the worm. Thus, types of biliary mucin were determined qualitatively at
various infection periods in both animal models. Paraffin-embedded biliary tissues of
Ov-infected golden Syrian hamsters and BALB/c mice were equally allotted into 6
subgroups for the examination on days 1, 2, 7, 14, 28, and 56 post infection (p.i). High
iron diamine-Alcian blue pH2.5-Periodic acid Schiff histochemistry was employed to
identify the mucin types. In mouse model, goblet cell metaplasia with non-sulfated acid
mucin was abundantly detected in their intrahepatic bile duct. Disappearance of Ov
occurred on day 14 p.i. In hamster model, production of sulfated acid mucin was
commenced on day 14 p.i. It indicates that only the non-sulfated acid mucin can
attenuate the Ov and play a pivotal contributor in the worm expulsion. Further
investigation is crucial to provide a clear mechanism in Ov parasitism of both mucin
types.
Keywords: mucin, hamster, mouse, Opisthorchis viverrini
206
Isolation, Identification of Bacteria from Vulva Swab of African Pygmy
Hedgehog (Atelerix albiventris) and Javanica Porcupine (Hystrix javanica)
and Antimicrobial Susceptibility Test
Anindya Dwi Ash-Santri1, Astari Kelana Hanindyani1, Vinsa Cantya Prakasita2, Yosua
Kristian Adi3, Teguh Budi Pitojo4, Agnesia Endang Tri Hastuti Wahyuni5*
1Studentin Faculty of Veterinary Medicine, University of Gadjah Mada

2Department Biology, Faculty of Biotechnology, Universitas Kristen Duta Wacana
3Department of Reproduction, Obstetrics and Gynecology, Faculty of Veterinary
Medicine, University of Gadjah Mada

4Department of Anatomy, Faculty of Veterinary Medicine, University of Gadjah Mada
5Department of Microbiology, Faculty of Veterinary Medicine, University of Gadjah
Mada
* Corresponding author: wahyuni_aeth@ugm.ac.id
Abstract
Atelerix albiventris and Hystrix javanica are currently traded as pets or consumed in
Indonesia, but there has been no research about bacteria from the vulva swab before.
This research aims to isolate, identify bacteria from the vulva swabs of Atelerix
albiventris and Hystrix javanica, and identify antibiotic susceptibility. Samples were
isolated by blood agar plates and selective media and identified by biochemical tests.
Kirby Bauer’s disk diffusion method was used for the antimicrobial susceptibility test.
The result showed that Atelerix albiventris was identified Escherichia coli (25%) and
Proteus mirabilis (75%), meanwhile Hystrix javanica was identified Escherichia coli
(100%). The identified Escherichia coli is sensitive to Amikacin, Amoxycillin, Ampicillin,
Enrofloxacin, Fosfomycin, Chloramphenicol, Tetracycline, Trimethoprim, and
Kanamycin; intermediate to Streptomycin; and resistant to Erythromycin and Penicillin
G. The identified Proteus mirabilis is sensitive to Amikacin, Amoxycillin, Kanamycin,
Enrofloxacin, and Fosfomycin; and resistant to Erythromycin, Penicillin G, Streptomycin,
Ampicillin, Chloramphenicol, Tetracycline, and Trimethoprim. This research concludes
that Escherichia coli and Proteus mirabilis is isolated from vulva swab of Atelerix
albiventris and Hystrix javanica, Escherichia coli is sensitive to eight antibiotics, while
Proteus mirabilis is sensitive to five antibiotics.
Keywords: susceptibility, antimicrobial, Atelerix albiventris, Hystrix javanica, Escherichia

coli, Proteus mirabilis
207
Isolation, Identification, and Antimicrobial Sensitivity Test of Bacteria
Isolated from The Rectal Swab of African Pygmy Hedgehog (Atelerix
albiventris) and Javan Porcupine (Hystrix javanica)
Astari Kelana Hanindyani1, Anindya Dwi Ash-Santri1, Vinsa Cantya Prakasita2, Yosua
Kristian Adi3, Teguh Budi Pitojo4, Agnesia Endang Tri Hastuti Wahyuni5*
1Studentin Faculty of Veterinary Medicine, University of Gadjah Mada

2Department Biology, Faculty of Biotechnology, Universitas Kristen Duta Wacana
3Department of Reproduction, Obstetrics and Gynecology, Faculty of Veterinary
Medicine, University of Gadjah Mada

4Department of Anatomy, Faculty of Veterinary Medicine, University of Gadjah Mada
5Department of Microbiology, Faculty of Veterinary Medicine, University of Gadjah
Mada
*Corresponding author: wahyuni_aeth@ugm.ac.id
ABSTRACT
Atelerix albiventris and Hystrix javanica were widely known as pets or livestock in
Indonesia, but there has been no study about bacteria from the rectal swab before. This
study aims to isolate, identify, and analyze the antibiotic sensitivity of the isolated
bacteria from the rectal swab of Atelerix albiventris and Hystrix javanica. Rectal swab
samples were cultured on blood agar plate and identified by selective media and
biochemical tests. Kirby Bauer’s disk diffusion method was used for the antimicrobial
sensitivity test. The result shows that the bacteria identified from the rectal swab
samples of Atelerix albiventris are Escherichia coli (75%) and Proteus mirabilis (25%),
meanwhile the bacteria identified from Hystrix javanica are Escherichia coli (100%). The
identified Escherichia coli found from the sample is sensitive to Amikacin, Amoxycillin,
Ampicillin, Enrofloxacin, Fosfomycin, Kanamycin, Chloramphenicol, Streptomycin,
Tetracycline, and Trimethoprim; and resistant to Erythromycin and Penicillin G. The
identified Proteus mirabilis is sensitive to Amikacin, Amoxicillin, Ampicillin, Kanamycin,
and Trimethoprim; intermediate to Enrofloxacin; and resistant to Erythromycin,
Fosfomycin, Chloramphenicol, Penicillin G, Streptomycin, and Tetracycline. This
research concludes that the bacteria found from the rectal swab of Atelerix albiventris
and Hystrix javanica are bacteria with similar species and characters.
Keywords: sensitivity, antimicrobial, Atelerix albiventris, Hystrix javanica, Escherichia

coli, Proteus mirabilis
208
Study on Parturisi of Beef Cows in Smallholder Farm: Stages, Placental
Weight, and Placentomes
Sri Gustari1, Surya Agus Prihatno1, Asmarani Kusumawati1, Agung Budiyanto1, Erif
Maha Nugraha Setyawan1, Yosua Kristian Adi1, Topas Wicaksono Priyo Jr1

Universitas Gadjah Mada, Indonesia 55281
ABSTRACT
This research aimed to study parturition stages, placental weight, and placentomes
number on beef cattle in smallholder farm. The research was a field study that carried
out on various breed of beef cows. The cows were at least in 9 months of pregnancy.
Observation was done for 24 hours every day to monitor parturition sign of each stage
until parturition has completed. Data collected were duration of each stage, placental
weight, also the number of placentomes, then analyzed descriptively. There were 32
beef cows participating in this research, consisted of 21 PO, 7 Simpo, and 4 Limpo cattle.
This research found that the first stage in all breed was longer compared to others
references, on the other hand the second and third stages were similar. In Simpo and
Limpo cows had more placentomes number compared to PO cows. On the contrary
placental weight in Simpo and Limpo cows were lighter than PO cows.
Keywords: beef cow, parturition, placental, placentome
209
A Comparison of Three Spectrophotometric Methods for Protein
Concentration Determination of Soluble Trypanosoma Antigen
Zul Azmi1,2, Fitrine Ekawasti2, Muhammad Ibrahim Desem2 and Didik Tulus Subekti2
1Goat Research Station, Ministry of Agriculture, 20585 Galang, Indonesia
2Indonesia Research Center for Veterinary Science, Ministry of Agriculture, 16124

Bogor, Indonesia
ABSTRACT
Soluble Trypanosoma antigen (STrAg) has been widely used as an antigen on the
development of trypanosomiasis detection kit. But, comparison of effective and efficient
protein quantification method has not been widely published. This study aims to
compare three different colorimetric protein assays (Bradford, Lowry, and bicinchoninic
acid or BCA assays) for measuring protein in five batches STrAg of Indonesia’s
Trypanosoma evansi isolate. These samples gave different results. The mean value of
protein quantification based on Bradford assay was 0.99 µg/µl with 22 visualized bands
on SDS-PAGE. Meanwhile, Lowry and BCA assays have a mean value of 2.28 µg/µl with
18 visualized bands and 4.67 µg/µl with 15 visualized bands, respectively. By the
comparison of the protein profile on SDS-PAGE with its protein value, Bradford assay
was more accurate and consistent than Lowry and BCA assays. Thus, we recommend
Bradford assay for measuring STrAg protein concentration.
Keywords: soluble Trypanosoma antigen, protein, Bradford, Lowry, bicinchoninic acid
210
Identification of Lactobacillus spp. on Basis Morphological, Physiological,
and Biochemical Characteristic from Jawa Super Chicken Excreta
Dini Dwi Ludfiani1 , Widya Asmara2,* , Agnesia Endang Tri Hastuti Wahyuni2 , and
Pudji Astuti3
1Student
in Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta,
Indonesia

3Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
*Department
ABSTRACT
Background: Ban the use of antibiotic growth promoter as a feed additive for animals
in Indonesia has regulated in law and starting from January 1, 2018. Alternative
replacement for antibiotic was investigated and introduced to farms such as probiotic.
The commonly used probiotic were the genus Lactobacillus, Bifidobacterium, and
Bacillus. Objective: The aim of this study was to identify the Lactobacillus strains isolates
from duck excreta. Identification was based on morphological, physiological, and
biochemical characteristic. Methodology: Sample was collected from ducks that
intensively. Each sample was diluted in 0.9% sterile saline and plated on MRS agar
containing bromocresol purple. All plates were incubated at 37°C under anaerobic
condition. Isolates were identified morphology, physiology, and biochemistry based on
fermentation of carbohydrate by using Analytical Profile Index (API 50 CHL). Result: This
study showed that isolates belong to 2 categories of fermentation, facultatively
heterofermentative and obligately heterofermentative. Not all isolates can grow at
40°C, but survive to low pH. Based on biochemical analysis isolates identify as L. brevis
and L. pentosus. Isolates Conclusion: L. brevis and L. pentosus were strains of the
Lactobacillus found in the excreta.
Keywords: Probiotic, Lactobacillus, Jawa Super Chicken Excreta
211
Ocimum sanctum Linn. Ethanolic Extract prevents apoptosis on the in-
vitro model neurodegeneration induce by Thrymethiltin
Dinda Aliffia1, Dinda Ayu Ramadhani1, Puspa Hening2, Widya Wasityastuti3, Dwi Liliek
Kusindarta1 and Hevi Wihadmadyatami1*
Yogyakarta, 55281
2Integrated Laboratory for Research and Testing, Universitas Gadjah Mada, Yogyakarta,
Indonesia, 55281
3Department of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
Indonesia, 55281
ABSTRACT
Neurodegenerative disease is the progressive atrophy or apoptosis of central nervous

system neurons due to loss of function or damage to neurons. The combination of
neuroprotectant and antioxidant functions in the active compounds in ethanolic extract
Ocimum sanctum Linn can be used as a drug candidate for neurodegenerative diseases.
This study aimed to analyze the Ocimum sanctum ethanolic extract's anti-apoptotic role
in the in vitro model neurodegenerative disease. The PC-12 cells were cultured in
DMEM, then the cells seeded on the six-well plate in the presence of TMT, OSE, and
Donepezil HCl. Besides, we also perform an in silico analysis. Our results show the OSE
can protect the PC12 cell line mediated by downregulation of the inflammation factor.
Keywords: Ocimum sanctum Ethanolic Extract, Neuroprotective, Apoptotis, PC12
212
The Effect Of Curcuminoid Temulawak Extract In White Rats Which
Inducted With Atherogenic Feed Toward Total Plasma Cholesterol
Levels
Nyi Raden Nokeu1 , Jenifer Margarita1, Rini Widayanti2, Aris Purwantoro2, Sarmin3,
Claude Mona Airin3, Ariana4, Soedarmanto Indarjulianto5, Trini Susmiati2
1 Undergraduate Student of Veterinary Faculty, Universitas Gadjah Mada

2 Department of Biochemistry, Veterinary Faculty, Univerditas Gadjah Mada
3 Department of Physiology, Veterinary Faculty, Universitas Gadjah Mada
4 Department of Anatomy, Veterinary Faculty, Universitas Gadjah Mada
5 Department of Internist, Veterinary Faculty, Universitas Gadjah Mada
The aimed to determine the effect of 15 µg/mouse and 20 µg/mouse curcuminoid

temulawak extract on white rats (Rattus norvegicus) induced with atherogenic feed on
plasma total cholesterol levels. Fiveteen white rats within aged of three months were
divided into three groups, namely group A as negative control fed normal feed, group B
as positive control group fed with 10% atherogenic feed, and group C as treatment group
fed atherogenic 10% and curcuminoid temulawak extract at a dose of 15 µg/mouse and
20 µg/mouse. All groups were adapted for two weeks, followed by treatment for two
months. Blood drawn at the day 0, first month, and second month through the optic
vein. Total cholesterol levels were measured using the cholesterol kit Enzymatic
Endpoint (CHOD-PAP) method. The data analyzed using ANOVA test and Post Hoc tests.
The results indicated there is no decrease in total cholesterol levels after curcuminoid
inhibition. This study concluded that curcuminoid temulawak extract at a dose of 15
µg/mouse and 20 µg/mouse within 1 up to 2 months can not inhibit the increasing of
total blood plasma cholesterol levels of induced atherogenic feed rats.
Keywords: total cholesterol level, temulawak, atherosclerosis
213
Antimicrobial Sensitivity Pattern of Staphylococcus sp, Bacillus cereus,
Burkholderia mallei, Acinetobacter calcoaceticus baumanii complex, and
Nesseria sp Isolated from Dogs with Dermatitis
Marla Anggita1 , Yovina Meideline Hendrata2 , Agnesia Endang Tri Hastuti Wahyuni1*

2Practicioner of Doctor Veterinary Medicine, Indonesia
ABSTRACT
Dermatitis is a common disease affecting dogs which most of the cases is caused by
bacteria. Antibiotics are used to treat bacterial infection. Misused of antibiotics and
long-term treatment can trigger antibacterial resistance that can cause sub-optimum
treatment. The purpose of this study is to know the antibiotic sensitivity of the bacteria
isolated from dogs with dermatitis. Isolates of Staphylococcus aureus, Staphylococcus
pseudointermedius, Bacillus cereus, Burkholderia mallei, Acinetobacter calcoaceticus
baumanii complex, and Nesseria sp. from dogs with dermatitis was re-identified by gram
staining, colony morphology, and biochemical test. Each bacteria then cultured on
Müller Hinton Agar (MHA). Antimicrobial sensitivity of each bacteria was done using
Kirby-Bauer method against 8 antibiotics: enrofloxacin, chloramphenicol, trimethoprim,
oxytetracycline, kanamycin, erythromycin, amikacin, and ampicillin. The study showed
that Burkholderia mallei and Neisseria sp. were sensitive to 6 of 9 antibiotics,
Staphylococcus aureus was sensitive to 4 of 9 antibiotics, Bacillus cereus was sensitive
to 3 of 9 antibiotics, Staphylococcus pseudointermedius was sensitive to 3 of 9
antibiotics, and Acinetobacter calcoaceticus baumanii complex was sensitive to 1 of 9
antibiotics. Amikacin has high sensitivity against all bacteria isolates.
Keywords: antimicrobial, sensitivity, dermatitis, dog
214
Hematology Profile of Equine Non-specific Colic Case in Yogyakarta
Province
Dinar Arifianto1*, Dorothea Vera Megarani1,

Christin Marganingsih Santosa1, Agus Purnomo2, Siti Isrina Oktavia Salasia1
Department of Clinical Pathology, Faculty of Veterinary Medicine,

Department of Surgery and Radiology, Faculty of Veterinary Medicine,
Jl. Fauna 2, Karangmalang, Yogyakarta 55281
*email: dinar.arifianto@ugm.ac.id
ABSTRACT
The cases of equine digestive tract disorders are very high. One of them that become
number one killer is colic. Colic can be defined as pain that come from the abdominal
area. Anatomical and physiological factors play a major role for the occurrence of colic
in horse. Blood tests and other laboratory examinations are very important to confirm
the diagnosis, support physical examination, and determine a treatment plan. This study
discussed about hematology profile in equine colic cases in Yogyakarta province. Horses
that showed colic symptom were examined and blood sample were taken from jugular
vein. Faeces were collected from rectal palpation. Blood samples were examined at the
Department of Clinical Pathology, Faculty of Veterinary Medicine UGM. The blood
profiles that were examined included RBC, PCV, Hb, platelets, WBC and leukocyte
differentials. The results showed that 57.7% (15/26) of the horses were accompanied
with anemia, 30.7% (8/26) of horses had thrombocytosis, while 7.7% (2/26) had
thrombocytopenia. Leukocytosis occurred in 26.9% (7/26) of horses, whereas
leukopenia occurred in 7.7% (2/26) of horses. 15.3% (4/26) horses had lymphopenia,
3.8% (1/26) horses had lymphocytosis, 7.7% (2/26) horses had neutropenia, 19.2%
(5/26) horses had neutrophilia, and 7.7% of horses had eosinophilia. Hematological
features of equine colic are diverse and it is difficult to find a distinctive pattern, but
from the data obtained, more than 57% of the patients are accompanied by anemia.
Other parameters (PCV, platelets, WBC, and leukocyte differentials) can follow the
physiological changes that occur in patients.
Keywords: Hematology, colic, horse, Yogyakarta
215
The ability of Ocimum sanctum Linn. ethanolic extract as A
Neuroprotectant on the in vitro model neurodegenerative (SHSY5Y)
Dinda Ayu Ramadhani1, Dinda Aliffia1, Puspa Hening2, Widya Wasityastuti3, Dwi Liliek
Kusindarta1 and Hevi Wihadmadyatami1*
Yogyakarta, 55281
2Integrated Laboratory for Research and Testing, Universitas Gadjah Mada, Yogyakarta,
Indonesia, 55281
3Department of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
Indonesia, 55281
*Corresponding author :heviwihadmadyatami@ugm.ac.id
ABSTRACT
Neurodegenerative disease is a progressive disease caused by neuronal apoptosis or

atrophy in the central nervous system. The availability and the cost of
neurodegenerative disease drugs are common problems among patients. Besides, the
effectiveness of the drugs is limited. Hence, alternative medicines for
neurodegenerative diseases are compulsory. Ocimum sanctum Linn, a prominent herb
in traditional medicine, was reported by the prior study that it has neuroprotectant
properties. Thus, it is potential to be the alternative drug for neurodegenerative
diseases. This study will explain Ocimum sanctum Linn's ethanolic extract (OSE) activity
to inhibit neuronal apoptosis on the in-vitro model and in-silico analysis. In this study,
neuroblastoma (SH-SY5Y) cell lines were induced with TMT as a neurodegenerative
model. The cells were cultivated using DMEM/F12K medium then treated with OSE and
Donepezil HCl. Our results show the ability of OSE to prevent the apoptosis of neuron by
inhibiting caspase-3 pathway.
Keywords: Neurodegenerative, Ocimum sanctum, SHSY5Y, apoptosis
216
PCR Detection of Avibacterium paragallinarum from Layers in Sleman
District, Special Region of Yogyakarta, Indonesia
Ima Fauziah 1, Widya Asmara 2, , Agnesia Endang Tri Hastuti Wahyuni 2, and Rini
Widayanti 3
1Student of Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine,

Universitas Gadjah Mada, Yogyakarta 55281, Indonesia

ABSTRACT
Infectious coryza (IC), caused by Avibacterium paragallinarum, is an infectious and

contagious respiratory tract disease that affects the commercial poultry industry.
Molecular techniques have been developed to detect A. paragallinarum and mostly use
species specific PCR, HPG-2 PCR. The current study was carried out to isolate A.
paragallinarum followed by PCR confirmation of identified bacteria from layers with
infectious coryza signs in Sleman District, Special Region of Yogyakarta. Nine field
isolates were observed for the growth of colony and cell morphology then characterized
biochemically (catalase, oxidase, urease, indole, motility, and carbohydrates
fermentation). Out of 9 isolates, 6 isolates were identified as A. paragallinarum then
confirmed by species specific HPG-2 PCR for A. paragallinarum. All 6 field isolates were
confirmed as A. paragallinarum.
Keywords: Avibacterium paragallinarum, infectious coryza, layer, PCR.
217
Association of Canine External Otitis Cytological Findings and Its
Causative Agents: a Mapping Review
Dewananda Dion Adiriesta1, , Souza Clarissa Pimentel De2 , and Salasia Siti Isrina
Oktavia1
1Universitas Gadjah Mada, College of Veterinary Medicine, Clinical Pathology
Department, 55201, Yogyakarta, Indoensia

2University of Illinois at Urbana-Campaign, Veterinary Clinical Medicine Department,
Veterinary Teaching Hospital, 61802, Illinois, United States of America
ABSTRACT
Canine otitis externa is an acute/chronic inflammatory disease of the external ear canal
that has a multifactorial etiology and predominantly caused by microbial infections.
Clinical signs include: exudates, erythema, oedema, offensive odour and pruritus.
Cytological evaluation of otic exudate is a useful diagnostic test to direct and monitor
treatment for otitis externa. It is thought that there may be direct correlations on the
parameters that are found in cytological evaluation and the type of external otitis
causative agents. A specific grouping correlations of the active leucocytes, ear canal
cells, the shape and coloration effects of the agents could lead to a more accurate
predictive definitive caustic agents. Veterinary medicine should favour to a more specific
therapeutic compunds to reduce the growing incidence of global antimicrobial
resistances. The most common bacteria isolated from the ear canals affected by otitis
are Staphylococcus spp. This bacteria infection could be identified by the clear grape-
like shape with predominatly active neutrophils and some histiocytes in the findings.
Other bacteria commonly associated with oititis with different identifiable shapes
include Pseudomonas, Proteus, Enterococcus, Streptococcus, and Corynebacterium.
Malassezia yeast is another common agent of otitis externa in dogs with specific
cytological findings.
Keywords: Otitis, Cytology, Staphylococcus, Malassezia
218
In vitro Anthelminthic Activity of Aqueous Extract from Sengon Laut
(Paraserianthes falcataria) Seeds Against Haemonchus contortus
Zein Ahmad Baihaqi1, Irkham Widiyono2,*, Wisnu Nurcahyo3, Bambang Suwignyo4

and Amado Angeles5
1Studentof Doctoral Program, Faculty of Veterinary Medicine, Universitas Gadjah


4Department of Animal Nutrition, Faculty of Animal Science, Universitas Gadjah Mada,
5Animal and Dairy Sciences Cluster College of Agriculture, University of the Philippines
Los Banos, Philippines

*Corresponding author: irkhamwidiyono@ugm.ac.id
ABSTRACT
The study was conducted to determine the potential of extract aqueous of sengon laut
seeds against Haemonchus contortus. Concentration extract aqueous of seed (E.A.S.)
sengon laut (0, 0.5, 1, 2.5 and 5 %) placed in separate Petri dishes (50mm); 20
Haemonchus contortus adult worms placed in petri dishes and incubated at 37°C, for 0.5,
1, 2, 3, 4, 5 to 6 hours. Tests were repeated 3 times on different days and mortality of
each worm was ensured by pressing the body of the worm with a pair of tweezers and
keeping it in lukewarm water for 5 min before declaring it dead. The parasites were then
observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv.
Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed
by Tukey’s test to detect significant differences (p<0.05). In vitro study of E.A.S. sengon
laut showed significant effect (P=0.00) against to suppress Haemonchus contortus, E.A.S.
sengon laut has suppress of Haemonchus contortus 100% at a concentration of 5% on
5th. SEM analysis of the worm treated with E.A.S. showed damaged cuticle structure.
E.A.S. sengon laut demonstrated anthelmintic activity against Haemonchus contortus.
Keywords: Anthelmintic, seed, Haemonchus contortus, Paraserianthes falcataria,

scanning electron microscopy.
219
Analysis of Blood Draining Completeness on Femoral (drumstick) Broiler
Chickens Colorimetric Based by Digital Image Processing
Koekoeh Santoso1 , * Syahrul Fadillahir Rachmad 2 , Pudji Achmadi 1 , Ridi Arif 3 , Agik
Suprayogi 1 , Mokhammad Fakhrul Ulum 4 , Kudang Boro Seminar 5 , Hadri Latif 3 ,
Herwin Pisestiyani 3
1
Veterinary Medicine, Department Anatomy, Physiology and Pharmacology, 16680 Dramaga
Bogor, Indonesia
2
Veterinary Medicine , DVM Education Program, 16680 Dramaga Bogor, Indonesia
3
Veterinary Medicine, Department of Veterinary Science And Veterinary Public Health, 16680
Dramaga Bogor, Indonesia
4
Veterinary Medicine, Department of Veterinary Clinic Reproduction and Pathology, 16680
5
Agricultural Technology, Department of Mechanical and Biosystem Engineering, 16680
ABSTRACT
An abuse of selling slaughtered dead animal meat cases nowadays has increase people
awareness because of food safety and halal reasons. Broiler chicken meat traded in
traditional markets is generally cut directly, namely the chicken is slaughtered in the
neck area, then it is done or without draining. The cutting process is considered to cause
imperfect blood loss. The presence of blood in the meat can cause the quality of the
chicken carcass to decline and the meat to rot quickly. The sale of chicken meat that
comes from dead chickens before slaughtering, also known as tiren chicken, also occurs
in the market. One method that can be used to determine which animals die before
being slaughtered is through measuring blood levels in meat using image processing
with ImageJ. This study purpose to analyze the potential of the image scanner using
ImageJ compared with spectrophotometer to measure the blood concentration by
colorimetric-based in broiler chicken meat. Extracts of meat samples reacted with
malachite green and H2O2 were analyzed using a spectrophotometer and scanner.
Blood concentrations in slaughtered chicken meat accompanied by hanging, without
hanging and 8-hour carcasses had significant differences (P0.05) both measured by
spectrophotometers and scanners Correlation coefficient of spectrophotometer
standard solution absorbance with scanner image processing is 0.98 Based on these
results it can be concluded that the image of a scanner can be used to analyze the
perfection of blood draining in chicken meat. Research can be done as an application to
detect chicken tiren cut chicken meat found in traditional markets or supermarkets. The
slaughter of the chicken should be accompanied by hanging so that the blood comes out
naturally perfect.
Keywords: blood drain, chicken meat, colorimetry, scanner
220
Effectiveness Of Povidone Iodine And Antibiotic Treatment On
Postpartum Reproductive Disorders In Beef Cattle
Erif Maha Nugraha Setyawan1 , Yosua Kristian Adi1, Sri Gustari1, Asmarani
Kusumawati1, Agung Budiyanto1, Surya Agus Prihatno1
1Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No.2,

ABSTRACT
This research was conducted to find out the reproductive disorders of postpartum and
the effectiveness of its treatment in various nations of beef cattle. A total of 30 beef
cattle raised in cattle groups in the D.I.Y. area was used as research samples. Cows are
divided into three groups namely control group, iodine povidone infusion group, and
antibiotic infusion group. First, second, and third months postpartum are performed
clinical examination, blood collection, and rectovaginal examination. Haematological
and clinical conditions in postpartum beef cattle have variations but most show a high
profile of leukocytes in 1st to 5th week. Povidone iodine infusion treatment in 1st week
postpartum can reduce bacterial infection until 5th week and can accelerate the uterine
involution process during the recovery period.
Keywords: postpartum, cows, iodine povidone infusion, antibiotic infusion
221
The Mice’s Hematological Effect of given the Staphylococcus aureus and
Persea Americana
Christin Marganingsih Santosa1 , Dorothea Vera Megarani1 , Dinar Arifianto1, and Siti
Isrina Oktavia Salasia1
1Clinico Pathology Department, Faculty of Veterinary Medicine, University of Gadjah
Mada, Indonesia
ABSTRACT
Staphylococcus aureus is often the cause of various diseases and food poisoning in
animals and humans. Avocado seeds (Persea americana, Mill) contain important
compounds that have antimicrobial activity. This study aimed to observe the
hematological dynamics of mice (Mus musculus domesticus) infected with S. aureus and
induced by P. americana extract. Balb-C male mice aged 12 weeks were divided into 6
groups of 5 in each group, named K1, K2, K3, K4, K5, and K6. All groups were infected
with S. aureus 1x108 CFU / mL intraperitoneally (IP) on day 0. After the blood sample was
taken, then K1 was given a single dose of tetracyclin 1000 mg/kg BW, K2 was given of
1000 mg/kb BW of erythromycin, K3 was given aquabidest (placebo), K4 was given
P.americana extract of 300 mg/kg BW, K5 at a dose of 600 mg/kg BW and K6 at a dose
of 1200 mg/kg BW. The treatment was given for 7 consecutive days, then a second blood
sample was taken, analyzed with the VetScan-HM5 analyzer and interpreted. The results
of the first examination, the total range of erythrocytes were 6.33 - 11.78.1012 cells/L,
hemoglobin levels 5 - 15.8 g/dL, hematocrit values 23.48 - 51.92 %, total leukocytes 1.96
- 10.33,109 cells/L. The results of the second examination on total erythrocytes (.10 12
cells/L) at K1, K2, K3, K4, K5, K6 were 7.17 – 9.15. ; 7.61 - 9.7; 6.19 - 11.07; 8.3 - 9.79;
4.84 - 10.49; 8.87 - 10.37 respectively. Examination of hemoglobin levels (g/dL) were 9.3
- 12.8; 9.8 - 13.5; 8.6 – 14.6; 11.3 - 12.9; 6.5 - 14.1; 12.3 - 13.5 respectively. The
examination of the hematocrit value (%) were 31.69 - 44.34; 35.48 - 45.87; 31.83 - 49.67;
39.65 - 45.96; 24.0 - 46.89; 41.59 - 46.62 respectively. The total leukocytes (109 cells/L)
for K1, K2, K3, K4, K5, K6 were 5.78 - 11.39; 3.76 - 11.2; 5.73 - 11.2; 3.48 - 8.45; 4.65 -
11.8; 3.25 - 8.2 respectively. It was concluded that S. aureus infection caused leukopenia
and was potentially followed by lymphopenia, neutrophilia, and monocytosis. The
occurrence of microcytosis can lead to anemia, which can be categorized as microcytic
hypochromic or normocytic normochromic. Administration of P. americana extract at a
dose of 300 mg/kg BW (K4) for 7 days after S. aureus infection was found to be effective
in improving of hematological values in mice back to normal.
Keywords: Staphylococcus aureus, Persea americana, Mus musculus domesticus,
erythrocytes, leukocytes
222
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Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia

Asian Association of Veterinary Schools (AAVS)

OIE Regional Representation for Asia and the Pacific

FACULTY OF VETERINARY MEDICINE

ASIAN ASSOCIATION OF VETERINARY SCHOOLS (AAVS)

OIE REGIONAL REPRESENTATION FOR ASIA AND THE PACIFIC

Dear distinguished speakers, participants, ladies, and gentlemen,

Prof. Ir. Panut Mulyono, M.Eng., D.Eng.

Prof. Dr. Siti Isrina Oktavia Salasia, DVM

I am Masatoshi Hori, Head of the Department of Veterinary Medical Science, the

Prof. Masatoshi Hori, DVM., Ph.D.

DAY 1: Sunday, March 28th 2021

TIME (GMT+7) PROGRAM PIC

Experience sharing by AAVS Members

Day 2 1st ICAVESS 2021 Thematic Session Class

Room Meeting ID Pass Meeting link

*Presenter should be join the parallel room 15 minutes

No. Time Paper Title Name Presenter

1 13.00-13.08 38-008 Acute leptospirosis in working dogs in Jakarta Ambar A-001

2 13.09-13.16 38-014 Trematode and Nematode gastrointestinal Fitrine A-002

3 13.17-13.24 38-107 The Study of Immunity Duration Against Ambar A-003

13.25-13.31 Q and A session

4 13.32-13.40 38-049 A Brief Discovery of the Plerocercoid Larvae of Gloria A-004

5 13.41-13.49 38-072 Sero-surveillance and potential risk factors Saifur A-005

13.59-14.05 Q and A session

14.33-14.39 Q and A session

11 14.49-14.57 38-094 Effect of D-galactose on Weight Gain in Animal Dwi G1-016

12 14.58-15.06 38-095 Dermatophytosis in Cats: Clinical Signs and Sitarina G1-017

15.07-15.13 Q and A session

14 15.23-15.31 38-099 Detection of Ovine Herpesvirus-2 in Clinical Cases Harimurti G1-019

15 15.32-15.40 38-104 Antibacterial Activity of Lactobacillus spp. Allysa G1-020

15.41-15.47 Q and A session

No. Time Paper Title Name Presenter

1 13.00-13.08 38-013 Diversity of Macro invertebrate as an Evaluation Pranatasari B-001

3 13.17-13.24 38-024 The Biological Variation, the Index of Yeremia YS B-003

13.25-13.31 Q and A session

5 13.41-13.49 38-037 Ex-situ Exploration of Cemani Chicken in Balai Tatan B-005

6 13.50-13.58 38-043 Diversity of Echinoderms in Intertidal Zone of Salma Dewi B-006

13.59-14.05 Q and A session

7 14.06-14.14 38-053 The Locomotion Study of Bekantan (Nasalis Hery B-007

8 14.15-14.23 38-063 Micromorphology Digestive Tract of imor Lizard Eman B-008

14.33-14.39 Q and A session

12 14.58-15.06 38-074 Ichthyodiversity of Nglambor Beach’s Intertidal Rizka Apriani B-012

15.07-15.13 Q and A session

14 15.23-15.31 38-086 Correlation Between Foot Circumference and Marcel B-014

15 15.32-15.40 38-092 Dynamics of Diversity Decapods in the Intertidal Wahyu B-015

15.41-15.47 Q and A session

Cadmium Levels in Muscle Tissue of Three

Identification of Physical Properties, Nutritional Trisnawati

Antimicrobial resistance profile of local isolates

13.25-13.31 Q and A session

Supplementation of Herbals on the Production

Detection and Testing of Antibiotic Resistance of

13.59-14.05 Q and A session

Macroscopic Pathomorphology of Intravitality

Supply Chain and Potential Pathogens in Frog Sugiyono

14.33-14.39 Q and A session

Prediction of Body Weight from Body

Performance of Broiler Chickens Supplemented Yenny Niken

The Increase of Ruminant Population Based On

15.07-15.13 Q and A session

The Tenderness Quality of Sinjai Superior