BASICS OF

BIOENZYMES
Bioenzyme Entrepreneurs Academy

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ABOUT BIOENZYMES
Bioenzymes are a wonderful gift from the
nature. Obtained by fermenting the fruit
peels, they can be used for a versatile range
of applications including domestic
cleaning, agriculture, treating urban &
industrial waste, animal husbandry and so
on!
Bioenzymes are environment-friendly and
has no polluting effects!
Dr. Rosukon Poomponvang of Thailand is the
inventor Bioenzymes. She is a multi-faceted
personality with over 40 years' experience in
research and development of Bioenzymes.
Recipient of many international awards, Dr.
Rosukon is a renowned Doctor, Researcher,
Natural Healer, Acupuncture Therapist,
Natural Farmer and a Nun. Her contribution
to healing the world using of Bioenzymes is
immense.
HOW TO PRODUCE BIOENZYMES?
Bio-Enzyme is a complex solution obtained
by the fermenting the organic material with
sugar and water. You can use any suitable
organic material like flowers, corns, fruits,
vegetables, leaves, etc. However, following
combinations are recommended.
Fresh kitchen waste
1 (fruit or vegetable peels)
Sugar (white / brown sugar or
2 jaggery or molasses)
3 Water
WHAT ARE THE
USES OF BIOENZYMES?
Bioenzymes act as a multi-purpose agent
that can be used for a versatile range of
applications.
1. Household Cleaning - for cleaning the
home, kitchen, toilets
2. Personal Hygiene - for bathing, in
soaps, shampoos, conditioners
3. Washing - clothes, utensils, jewels, cars
4. Garden - as a plant growth booster and
anti-mildew
5. Industrial - for waste water treatment
and cleansing applications
6. Environmental - for cleaning lakes,
ponds, rivers
7. Agriculture - nutrients & soil health
booster
8. Civil Infrastructure - wet waste
management, sewage treatment plants
Based on the application, Bioenzymes will
need to be diluted. Each application has a
tried and tested dilution ratio.
HOW DO BIOENZYMES WORK?
Bioenzymes contain non-pathogenic,
'good' bacteria. These bacteria have the
property to digest the waste (grime, dust &
stains). They do so by producing enzymes
that are capable of breaking down waste
molecules in to smaller pieces (soil). These
smaller pieces become the food for the
bacteria. The bacteria consume the soil /
waste & break them in to two basic
compounds - carbon dioxide & water. The
bacteria grow in numbers and continue to
digest the waste until their food supply
diminishes (till the waste particles remain)
ARE BIOENZYMES SAFE?
Absolutely safe since they are composed of
non-pathogenic, 'good' bacteria.
In fact, Bioenzymes are safer for both
environment and human health as
compared to other conventional chemical
products! The micro-organisms have the
ability to penetrate into small cracks &
crevices to eliminate waste & odours which
normal chemicals can't. They can help
displacing disease causing pathogenic
bacteria!
Bioenzymes are safer for infants & pets too!
HOW BIOENZYME CAN
HELP THE ENVIRONMENT?
Earth's atmosphere is composed of about
• Nitrogen (N2) - 78%
• Oxygen (O2) - 21%
• Argon (A) - 0.9%
• Other gases such Carbon dioxide (CO2),
Methane (CH4), water vapor (H2O), and
Neon (Ne) – 0.1%
When exposed, Bioenzyme continuously
releases Oxygen (O2), Carbon dioxide (CO2)
and Nitrogen Oxide (N2O) into the
atmosphere.
This triggers a chain of chemical reaction
which produces earth-friendly compounds.
1. O2 from atmosphere combines with O
released by Bioenzyme to form
Ozone (O3)
• O2 +[O] -> O3
2. CO2 from atmosphere combines with
O2 released by Bioenzyme to form
Carbonate (CO3)
• CO2 + O2- -> CO32- + [O]
• The free Oxygen [O] helps by
oxidizing the hazardous
elements present in the
atmosphere
3. O2 from atmosphere combines with
Nitrogen Oxide (N2O) released by
Bioenzyme to form Nitrate (N2O3)
• N2O + O2 -> N2 O3
Ozone is known for reducing the greenhouse
gas effect. It absorbs solar ultraviolet
radiation, which heats the stratosphere. It
also absorbs infrared radiation emitted by
the Earth's surface, effectively trapping heat
in the troposphere.
Both Nitrates and Carbonates are good for
the environment, soil and marine life.
We use hundreds of products in our daily life.
From soaps to shampoos to cleaners at
home. Most of the products available in
market often contain harmful chemicals.
These chemicals are mainly responsible for
environment pollution including water and
soil contamination.
Byproducts made from Bioenzymes can
easily substitute hazardous, chemical
based products which we use in our daily
life! If we can turn our kitchens waste into
Bioenzymes, then we are reducing our
dependency impacting the environment
directly.
DO IT YOURSELF
YOU WILL NEED:

1. CONTAINER:
• Air-tight plastic container recommended

2. INGREDIENTS:
• Organic Matter (fruit / vegetable peels)
• Sugar (brown sugar / molasses sugar / jaggery)
• Water

Remember the Formula : 1+3+10

• 1 part of Sugar + 3 parts of Organic Matter + 10 parts of Water
• For example: 1 Kg of Sugar, 3 Kg of fruit / vegetable peels, 10 Litres of water

Steps:

01
Step 1
Add 10 Parts water
(Fill up 60% of the
container)
04
Step 4
Close the container
with the lid tightly. Keep
it for 90 days (open
daily to release gases
for the first 30 days)

02
Step 2
Add 1 Part sugar
(10% of water content)
05
Step 5
Filter the contents. Store
the concentrated
Bioenzymes in plastic
containers. Dilute as
applicable for each use

03
Step 3
Add 3 Parts kitchen
waste
(Fill up to 80% full)
06
Step 6
Your Bioenzyme is now
ready for use!
TIPS

1. Use only air-tight plastic container. Do not use glass or metal containers since these
containers can't expand.

2. Dilute sugar in water, follow by adding fruit / vegetable peels. Use only Organic matter
(fruit / vegetable peels). You can add orange, lemon peel or pandan leaves etc to make
your enzymes smell fresh. Don't add cooked food, fish or meat residues

3. If you have not gathered enough organic material, don't worry. You may fill the container
gradually. The 3 month fermentation period start from the day you place your last batch
of organic material

4. Leave some space in the container for fermentation. Ensure to close the lid tight. Close the
lid tightly. Place the container in a cool, dry and well ventilated area. Avoid direct sunlight

5. During the first month, gases will be released during fermentation process. To avoid
rupturing of the container, you need to release the built up pressure by simply opening
the lid for 5 minutes daily (first 30 days only)

6. You may get a white, black or brown layer on top of the enzyme, ignore it. Occasionally,
you may also encounter flies or worms. Just leave it and the chemical reaction of enzyme
will take care of them naturally. You may choose Stir the contents occasionally so that
the organic content gets mixed up well

7. Fermentation will take at least 3 months (90 days) before bioenzymes can be used. The
ideal colour of garbage enzyme is dark brown. If it turns black, add in same amount of
sugar to start fermentation process again. Although 3 months are enough, Bioenzymes
work best after 6 months of fermentation. The longer it takes, the better it gets

8. Filter concentrated Bioenzymes and store them in plastic containers. Do not store in the
refrigerator. Before use, dilute the concentrated bioenzymes, as applicable

9. You can reuse the residue (remaining organic matter in the container) for next batch. Or
you can use it as a fertilizer by just drying before applying on the soil

10. Always remember - Bioenzyme has no expiry date!

QUICK REFERENCE GUIDE

Dilution Ratio
(Bioenzymes to Purpose Quantity Usage Instructions
Water)

Bath Keep it overnight in a bucket of

50–100cc
(add in bath water to improve skin) water before using

Washing machine Soak the clothes for few hours

20–50cc      
(clean and soften laundry) before hand washing

Toilet bowl Pour it scrub it and flush

No Dilution 250CC
(prevent blockage & purify sewage) Instantly
(Concentrated
Enzyme) Toilet system 20–50cc 2–3 times/weeks for good STP
(purify water) (two capful) functioning

Garden pond & water tank 1/10,000 litre Add occasionally to the
(purify water) water irrigation tank

Leather sofa
1:100 ratio Spray and wipe every 10 days
(clean mold or stain)

Carpet & tatami mat

Spray Spray and wipe every 15 days
(deodorise & antiseptic)

Shoe, inside the car

Spray Spray once a month
(deodorise & antiseptic)

Kitchen sink, stove, cooker hood

10-50 times Spray Spray and wipe every week
(clean oily stain)

Black mold Soak and wipe as and when

Soak in 500ml
(antifungal) needed

Pet excretion, pet house

Spray Spray as and when needed
(deodorise & antiseptic)

Air-condition room
200 - 500 times Spray Once in a month
(humidifier, deodorise & antiseptic)

Bathroom sink Pour it around

leave it for few mins and wipe it
(clean) the sink

Cabinet and refrigerator

Spray Once in a month
(deodorise)

Drain Pour Daily, Spray once in a

Pour 10ml
500 times (prevent drainpipe blockage) month

keep it overnight in a bucket of

Pet
100 cc water before using, Spray upon
(bathing, deodorise & antiseptic)
bathing or before brushing

Toilet
250cc Flush occasionally
(clean, deodorise & antiseptic)

Indoor
Spray Spray everyday
500 - 1000 (purify air, deodorise & pest control)
times Closet, clothes
Spray Once in a month
(deodorise & antiseptic)

Seeding and planting Rinse the seeds once before

1000 times 10 ml
(fertiliser) sowing
Disclaimer : The content of this book has been distilled from years of experience and learning
from the community. Whilst every effort has been made to ensure accuracy, BEA makes no
warranty for the reliability of the information contained herein. The quality of bioenzymes
produced is subject to the input materials & process followed.

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