CHAPTER 3

PHOTOSYNTHESIS
 Autotroph

LESSON
LEARNING Structure of the chloroplast

O U T C O M E S
Photosynthetic pigments

Light Dependent Reaction

 Photosystem
 Non Cyclic Photophosphorylation
 Cyclic Photophosphorylation
 Chemiosmosis
 TYPES OF LIGHT DEPENDENT REACTION

Non Cyclic Cyclic

01 02
Photophosphorylation Photophosphorylation

45%
 CYCLIC
PHOTOPHOSPHORYLATION

01 02 Schematic diagram of
Primary cyclic
acceptor
Primary Fd photophosphorylation
acceptor Fd
Pq NADP+
NADP+ + H+
reductase
Cytochrome NADPH
complex 45%

Pc
Cyclic photophosphorylation generates
surplus ATP, satisfying
the higher demand in the Calvin cycle.
Photosystem I Some organisms such as purple sulfur
Photosystem II ATP bacteria .
Cyclic electron flow is thought to have
evolved before linear electron flow.
 CYCLIC
PHOTOPHOSPHORYLATION
STROMA

Electron Transport Photon

Cytochrome
Chain
complex 3 1
Fd e-
Primary
electron
acceptor

e-

e-
Pc e-
P700

4
Photosystem I
H+

THYLAKOID SPACE ATP

 CYCLIC
PHOTOPHOSPHORYLATION
1. A photon hits a pigment and its energy is passed among pigment molecules until it
excites the reaction center complex Photosystem I, P700.

2. An excited electron from P700 is transferred to the primary electron acceptor.

P700 oxidized to P700+ .

3. Each electron “falls” down an electron transport chain in a circle from the primary
electron acceptor of PS I to Fd(Ferredoxin) and then to cytochrome complex, next to
Pc (plastocyanin) and eventually to reaction center complex of PSI, P700 from which
it was ejected.

4. Energy released by the fall of electron, drives the creation of a proton concentration
gradient across the thylakoid membrane. Diffusion of H+ (protons) across the
membrane drives ATP synthesis.
 Different between

Non Cyclic Cyclic

01 02
Photophosphorylation Photophosphorylation

Involve Photosystem II (P680) and

Photosystem Involve Photosystem I (P700) only
Photosystem I (P700)
Yes Photolysis No

Yes Release Oxygen 45% No

LINEAR CYLIC
PS II  Primary e acceptor  Pq  Electron flow PS I  Primary e acceptor  Fd  Cyt
Cyt complex  Pc –> PS I Complex  Pc  PS I
Final electron PS I
NADP+
acceptor
NADPH and ATP Product ATP only
To be continued…
Thank You