Triladyl® & Biladyl®

Media for Bovine Semen Freezing

:::::::::::: Proven Standard for Bovine Semen Production.
Based on TRIS Buffer. Requires fresh egg yolk to add.

Your benefits
• Elaborate formula, based on TRIS

• Since over 30 years internationally established

• Very constant, optimal freezing results at a wide range of dilution ratios

• CSS certified version without antibiotics available

(Triladyl CSS® and Biladyl®)

• Produced under Minitube GMP production standard

Triladyl®, 250 g with antibiotics GTLS according

to the EC Directive 88/407 REF. : 13500/0250

Triladyl® CSS, 200 g without antibiotics, according

to CSS requirements REF. : 13500/1200

Biladyl®, fraction A, 49 g REF. : 13500/0004

Biladyl®, fraction B, 250 g REF. : 13500/0006

Subject to modifications, errors excepted. Illustrations similar.
Composition ::::::::::::
Antibiotic Supplement GTLS, for 2-step extender,
according to CSS protocol REF. : 13500/0005 Triladyl®, Triladyl® CSS and Biladyl® contain TRIS, citric
acid, sugar, buffers, glycerol, purest water and antibiotics
Antibiotic Supplement GTLS, for 1-step extender, according to the EU Directive 88/407 (Tylosin, Gentamicin,
08/2012

according to CSS protocol REF. : 13504/9000 Spectinomycin, Lincomycin). Triladyl® CSS contains no
antibiotics.

w w w. m i n i t u b e . d e
:::::::::::: Industry standard
Since Triladyl® and Biladyl® have been introduced to the market, they
have established themselves as the classic extenders in bovine semen
production. Triladyl® and Biladyl® are also used successfully with
ejaculates of several other species, e.g. ovine, caprine as well as with
many exotic species.
:::::::::::: Efficient production protocols
For the preparation of Triladyl® and Biladyl® distilled water and
250g fresh egg yolk are added to the concentrate. The ejaculates can
be diluted with a large range of variation in dilution ratio, without
influencing the fertility results. Triladyl® and Biladyl® are being used
successfully with low concentration dilution (below 15 million sperm
cells per dose), as well as for bovine ejaculates with low density.
:::::::::::: Production standard and QC
Raw materials are produced according to GMP and DIN ISO 9001 norms,
and are certified after Ph Eur, BP or USP standards. They are tested
according to international valid quality guidelines concerning the
testing of pharmaceutical substances and meet these requirements.
Each single component of each extender batch is tested chemically,
physically and spermatologically under specialized veterinarian
supervision for their adequacy for the semen conservation. Analysis
certificates and testing protocols constitute full traceability.
Mixing and bottling of the extender is performed under GMP
conditions in controlled atmosphere and documented with weighing
records. The complete mixture is tested again chemically and physically
for spermatologic adequacy as well as under practical conditions for
the suitability for semen conservation under specialized veterinarian
supervision, and by independent organizations.
:::::::::::: Certificates
A general quality certificate as well as a batch certificate are available
upon request.
w w w. m i n i t u b e . d e
Selected scientific publications ::::::::::::
Christensen P., Boelling D., Pedersen K.M., Korsgaard I.R., Jensen.
(2005): Relationship between sperm viability as determined by flow
cytometry and nonreturn rate of dairy bulls. J. Androl., Jan-Feb; 26
(1): 98-106
Dumont P. (2002): Adjusting the number of spermatozoa in mini-
straws by determination of the operative volume of bovine
semen. Theriogenology, Apr 1; 57(6): 1743-54
Herold, F.C., Gerber, D., Aurich, J.E. (2002): Influence of homolgous
seminal plasma on bovine epididymal semen frozen with Triladyl® or
AndroMed®. Wiener Tierärztliche Monatsschrift, 90, 8–61.
Nabiev, D., Gilles, M., Schneider, H., Mahabir, E., Koll, H., Schellander,
H. & K. (2003): AndroMed® versus Triladyl® - influence on functional
in vitro fertility parameters and IVP of frozen thawed bovine semen.
Vet. Med. Austria 90, Suppl. 1.
Herold, F.C., Gerber, D., de Haas, K., Noethling, J.O., Cooper, D., Theu-
nisen, W., Spillings, B. (2003): Comparison of three different media for
freezing epididymal sperm from African buffalo (Syncerus caffer)
and influence of equilibration time on the post-thaw sperm quality.
Theriogenology, 59, 393.
Herold, F.C., Aurich, J.E., Gerber, D. (2003): Epididymal sperm from Af-
rican buffalo (syncerus caffer) can be frozen successfully with Andro-
Med® and with Triladyl® but the addition of bovine seminal plasma is
detrimental. Therioenology, in press
Szasz F., Gabor G., Solti L. (2000): Comperative study of different me-
thods for dog semen cryopreservation and testing under clinical
conditions. Acta Vet Hung, 48(3): 325-33
Garde J.J., Soler A.J., Cassinello J., Crespo C., Malo A.F., Espeso G., Go-
mendio M., Roldan E.R. (2003): Sperm cryopreservation in three spe-
cies of endangered gazelles (Gazella cuvieri, G. dama mhorr, and G.
dorcas neglecta). Biol reprod., Aug; 69(2): 602-11. Epub 2003 Apr
16
Janett F., Keo S., Bollwein H., Hässig M., Thun R. (2004): Comparison
of AndroMed®, Bioxcell and Triladyl® extender for cryopreservation of
bull semen. Conference on Physiology and Pathology of Reproduc-
tion; Zürich, February 2005
Janett F., Fuschini E., Keo S., Thun R. (2005): Comparison of Andro-
Med® and TRIS-egg yolk extender for cryopreservation of buck se-
men. ESDAR Conference, Murcia, Sept. 2005
Complete publication list available on request.