Diff. vol.

Glucose/ Somogyi Conc. of Vol. of

Tube Distilled Na2S2O3.5H2O Na2S2O3.5H2O used
Sample reagent dextrose Na2S2O3.5H2
No. water (mL) (mL) for blank & glucose
solution (mL) (mL) (µL/mL) O (mL) used
conc.
initial final
1 0(Blank) 5 5 0 0 29.1 29.1 0
2 1 4 5 20 0 21 21 8.1
3 2 3 5 40 4 18.6 14.4 14.7
4 3 2 5 60 0 6.3 6.3 22.8
5 4 1 5 80 6.3 7.7 1.4 27.7
6 5 0 5 100 7.7 7.8 0.1 29
T1 5 0 5 8.1 18.6 10.5 18.6
T2 5 0 5 18.6 30.3 11.7 17.4
T3 5 0 5 30.3 43.2 12.9 16.2

Diff. vol. thiosulphate used for blank and glucose

35
conc. vs. Glucose conc.
Differences between volume of thiosulphate

30 f(x) = 6.05428571428571 x − 4.14

used for blank and glucose solution

R² = 0.963697590091226
25

20

15

10

0
0 20 40 60 80 100
Concentration of glucose