Roll No.: …………..

MALWANCHAL UNIVERSITY Paper Code:

Bachelor of Pharmacy- 6th Semester

Examination July 2023

Subject: Pharmaceutical Biotechnology Subject Code: BP 605T

Time Max.: 30 Min + 2 Hours and 30 Min Max Marks: 20+55=75
Instructions:-

Mark () the right answer

Q.1 Multiple Choice Questions- (20x1=20)

1. Direct administration of antibodies to increase the resistance to disease is called ……..
(a) Active immunization (b) Passive immunization (c) Amination (d) None of the above
2. The B-cells are responsible for the production of ……………
(a) Cellular immunity (b) Passive immunity (c) Humoral immunity (d) Active immunity
3. How many base pairs of recognition sequence will get by type-II restriction enzymes action?
(a) 4-6 bp (b) 25 bp (c) 1000 bp (d) Non of the above
4. Direct replacement of hydrogen atom on a given carbon in microbial transformation process is
called……………
(a) Epoxidation (b) Hydrolysis (c) Decarboxylation (d) Hydroxylation
5. Steroidal bioconversion is performed by ……. fermentation technique
(a) Submerged aeration (b) Surface fermentation (c) Solis-state fermentation (d) None of the above
6. HAT media contains …………… drug
(a) Amoxicillin (b) Streptomycin (c) Aminopterin (d) All
7. The transfer of genetic material from one cell to another by a bacteriophage is called ……
(a) Transduction (b) Transformation (c) Conjugation (d) Protoplast fusion
8. Mutagenic agents increase the rate of mutation and these mutations are called ……….
(a) Spontaneous mutation (b) Induced mutation (c) Both A & B (d) None of the above
9. The maximum production of penicillin is obtained ………… days
(a) within 4-5 days (b) within 3-4 days (c) within 2-3 days (d) within 1-2 days
10. …… is used for the protein denaturant during gel electrophoresis in western blotting technique
(a) NaCl (b) NaHCo3 (c) Sodium dodecyl sulphate (d) Calcium chloride
11. ………. organism is present in the BCG vaccine
(a) Mycobacterium tuberculosis (b) Mycobacterium leprae (c) Vibrio cholerae (d) E. coli
12. Which media is used in the inoculum preparation of vitamin B 12 fermentation?
(a) MS media (b) Bennett’s broth (c) White’s medium (d) LS media
13. The transfer of foreign gene into another cell is done by the following methods …….
(a) Transduction (b) Conjugation (c) Transformation (d) All the above
14. In the world wide …. fermentation technique is most widely used for the citric acid production
(a) Surface fermentation (b) Solis-state fermentation(c) Submerged fermentation (d) None
15. The HGPRT enzyme is present in ……… type of cells
(a) Antibodies (b) Myloma cells (c) RBC’s (d) None of the above
16. Palindromic sequence contain……..
(a) Blunt end sequence (b) Ligation (c) Complementary sequence (d) None of the above
17. ….. enzymes catalyse both dehydrogenation and reduction reactions in microbial transformation
(a) Isomerase (b) Amylase (c) Glucoamylase (d) Oxido-reductases
18. Bioreactors are used for the growth of ………. type of cells
(a) Eukaryotic cells (b) Prokaryotic cells (c) Antibodies (d) Myloma cells
19. Vitamin B12 is produced by …….. organisms
(a) Bacteria (b) Actinomycetes (c) Both A & B (d) None of the above
20. …… bacteria was used by F. Griffith (1928) in the bacterial transformation experiment
(a) Streptococcus pneumonia (b) Staphylococcus aureus (c) E coli (d) Streptococcus aureus
Roll No.: ………….. MALWANCHAL UNIVERSITY Paper Code:

Bachelor of Pharmacy- 6th Semester

Examination July 2023

Subject: Pharmaceutical Biotechnology Subject Code: BP 605T

Time Max.: 30 Min + 2 Hours and 30 Min Max Marks: 20+55=75
Instructions:-

1. All Questions are Compulsory

2. Attempt all questions strictly in serial order only
3. Draw well labeled & colored diagrams wherever necessary.

Q.2 Long answer questions (Attempt any two) 2x10=20

a) Write in detail the hybridoma technology.

b) Explain in detail different biotransformation reactions with special reference to steroids.

c) Give the details of penicillin production with flow diagram.

Q.3 Short answer questions (Attempt any Seven) 7x5=35

a) Bacterial transformation with Griffith’s experiment.

b) Techniques used for immobilization of enzymes.

c) Draw a flow sheet and explain the production of citric acid.

d) Bacterial vaccines.

e) General preparation and types of standard biological products.

f) Polymerase chain reaction (PCR).

g) Southern blotting technique.

h) Hypersensitivity reactions

i) Transduction.