Time (days) Cell Concentration (cells ml^-1 x 10^-6) Ln (Cell Concentration (cells ml^-1 x 10^-6))

0 0.45 -0.798507696217772
0.2 0.52 -0.653926467406664
0.5 0.65 -0.430782916092454
1 0.81 -0.210721031315653
1.5 1.22 0.198850858745165
2 1.77 0.570979546585738
2.5 2.13 0.756121979721334
3 3.55 1.26694760348732
3.5 4.02 1.39128190263093
4 3.77 1.32707500145992
4.5 2.2 0.78845736036427

Ln(x)=miumax+Ln(Xo)
y= mx+b
vel max 0.6651 1/días
td 1.0421698700345
 Cell Concentration (cells ml^-1 x 10^-6)
4.5

Cell Concentration (cells ml^-1 x 10^-6)

3.5 f(x) = 0.740911119375232 x + 0.388301599107475
R² = 0.741799502012373

2.5

1.5

0.5

0
0 0.5 1 1.5 2 2.5 3 3.5

Time (days)

Ln (Cell Concentration (cells ml^-1 x 10^-6))

1.5
Ln (Cell Concentration (cells ml^-1 x 10^-6))

f(x) = 0.665148028547331 x − 0.802265253493677

1 R² = 0.993275798969771

0.5

0
0 0.5 1 1.5 2 2.5

-0.5

-1

Time (days)
 (cells ml^-1 x 10^-6)

475

2.5 3 3.5 4 4.5 5

me (days)

n (cells ml^-1 x 10^-6))

93677

5 2 2.5 3 3.5

Time (days)
Time (h) Cell Concentration x (kg m^-3) Substrate Concentration s (kg m^-3) Ln(x)
0 0.2 25 -1.60943791
0.33 0.21 24.8 -1.56064775
0.5 0.22 24.8 -1.51412773
0.75 0.32 24.6 -1.13943428
1 0.47 24.3 -0.75502258
1.5 1 23.3 0
2 2.1 20.7 0.74193734
2.5 4.42 15.7 1.4861397
2.8 6.9 10.2 1.93152141
3 9.4 5.2 2.24070969
3.1 10.9 1.65 2.38876279
3.2 11.6 0.2 2.4510051
3.5 1.7 0 0.53062825
3.7 11.6 0 2.4510051

Vel max
Td

Yx/s
2.8 0.461002178649237 kg biomasa/kg sustrato
3
 Cell Concentration x (kg m^-3) Chart T
14 3
2.5
12
f(x) = 1.49900602596841 x − 2
2 R² = 0.999986575577151
10
1.5
f(x) = 2.94386468019263 x − 1.50249623455504
8 R² = 0.652279025750423 1
0.5
6
0
0 0.5 1 1.5
4 -0.5

2 -1
-1.5
0
0 0.5 1 1.5 2 2.5 3 3.5 4 -2

1.499 1/h
0.46240639 h
27.7443835 min

g biomasa/kg sustrato
 Chart Title

f(x) = 1.49900602596841 x − 2.25860744605791

R² = 0.999986575577151

0.5 1 1.5 2 2.5 3 3.5

Time (h) Cell Concentration (g l^-1 dry weight) Sugar Concentration (g l^-1)
0 0.02 9.87
1 0.03 9.77
2 0.03 9.38
3 0.042 9.45
4 0.21 9.02
4.5 0.33 8.55
5 0.35 9.12
5.5 0.38 8.09
6 1.13 7.58
6.5 1.95 6.24
7 3.66 1.3
7.5 4.09 1.14
8 4.23 0.05
8.5 4.07 0.02
9 3.85 0.015
9.5 2.66 0.018
10 2.42 0
11 2.03 0
12 1.54 0
14 1.89 0
16 1.45 0
24 1.67 0

12

10

0
0 5 10 15 20 25 30
2

0
0 5 10 15 20 25 30
 Nisin Concentration (IU ml^-1)
0 Cell Concentration
278 4.5

Cell Concentration (g l^-1 dry weight)

357 4
564 3.5
662 3
695 2.5
1213 2
1341 1.5
1546 1
1574 0.5
1693 0
0 5 10 15 20 25 30
1678
1793 Time (h)
1733
1567
1430 Sugar Concentration
1390 12
1305
Sugar Concentration (g l^-1)

10
1220
995 8
806
6
617
4

0
0 5 10 15 20 25 30
Time (h)

20 25 30
20 25 30
 Nisin Concentration (IU ml^-1)
2000
1800

Nisin Concentration (IU ml^-1)

1600
1400
1200
1000
800
600
400
200
0
20 25 30 0 5 10 15 20 25 30
Time (h)

20 25 30