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Fluorometry of Dna Intercalating Fluorophores
Fluorometry of Dna Intercalating Fluorophores
Amplicon Assessment
In an unlit hood and away from direct light, aliquot 199 μl ONE dsDNA Dye to the S tube and 200 μl
to all the rest
Transfer 1 μl of amplicon eluate or negative controls to the corresponding labeled sample tube with
ONE dsDNA Dye (from Step 6.3 Elution)
Vortex mix, spin down and let stand at RT for 2 mins
Before loading any tube, wipe tube surfaces with line-free wipes’ Out:
<Amplicon Conc’n> to
Calibration 8.1
Sample measurement
Load a sample tube on to the device, press down lid to measure and record amplicon concentration
NOTE NEC and NTC reactions that record <1 ng/ul are acceptable
IMPORTANT
Decontamination with UV and or DNAzap between sample batches is recommended