Content

1. Introduction.
2. Items / Equipment.
3. Tank Construction.
4. Tank Aeration.
5. Fish Suitable for Biofloc.
6. Items for Water Preparation.
7. Water Parameters.
8. Water Testing & Control.
9. Water Preparation.
10. Floc Development.
11. Fish Sanitization & Stocking.
12. Ammonia Control & C:N Ratio.
13. Feed Management.
14. Daily Routing in the Fish Farm.
15. Fish Important Disease & Control.
16. Harvesting and Selling.

2
 1) Introduction ( i f j p ; %& Biofloc
Biofloc
Heterotrophic Wastage Floc
Floc
Floc

Advantage
a) Biofloc

b)

c)

d)
FCR (Feed Conversion Ratio)

Disadvantage
a) 24

b) -
c) water Parameter
d) High Density

2) Items / Equipment ( Biofloc

Items ( ) use Parameter

3
 Biofloc
Biofloc
Biofloc Use
a) PH Potential of Hydrogen) Meter PH
Biofloc PH 6.8
b) Salinity Meter - Salinity
Biofloc Salinity -
- 0.5 ppt 1.5 ppt
c) TDS Meter

d) Digital Thermometer - Temperature

Biofloc Temperature 25°C
35°C
e) Digital Weight Machine - Biofloc
-
f) Ammonia Kit (TAN- Total Ammonia Nitrogen)- Biofloc
Biofloc

g) Nitrite No2 Kit - Biofloc Nitrite

Biofloc Nitrite
h) Nitrate (No3) Kit - Biofloc Nitrate

i) DO (Dissolved Oxygen) Meter/Kit -

Biofloc ppm
ppm

3) Tank Construction ( )- Biofloc Technology

4
 Biofloc Technology Round

Some Important Instruction for Tank Construction

1)
2) Sludge
3) inch feet
4) Drainage
sludge
,
10000

Biofloc

1.
2.
Tarpaulin Tank Round Tank :- Round
Diameter Height
Diameter Dm

1. Iron Mesh ( MM )
2.
3. (10 MM)
4.
5.
6.
7.

5
 1
meter

Round Tank Calculation Method

Volume v = r2 h ( = 3.14, r = radius h= height)

V = 3.14*(2)2*1m

V = 12.56 m3 ( m3 = 1000 Liter )

V = 12.56*1000

V = 12560 Liter Water

Round Tank Iron Mesh Calculation Method

Circumference of Circle =

c = 2*3.14*2

c = 12.56 m (1M= 3.28Feet )

c = 12.56*3.28

c = 41.20 Feet

41.20 Feet Iron Mesh feet Iron Mesh

mesh
10000 25000 30000

6
 5 10000 15000

Cement Tank :-
Biofloc

1.
2.
3.
4.
5.

Rectangle and Square Tank Calculation Method

Volume v = Length*Width*Height

= 10*10*4

= 400*28.3

= 11320 Liter

10000 30000 35000

15 20 , ,

4) Tank Aeration Biofloc

Aeration Biofloc

7
Biofloc

Important Point of Air System-----

1) Floc Biofloc System

2) Aeration Sludge

3) Biofloc
4) Biofloc ppm ppm

5) 10000 Catfish LPM ( Liter Per

Minute LPM Pressure 20 KPA Kilopascal Air Pump

6) 10000 IMC LPM ( Liter Per

Minute LPM Pressure 20 KPA Kilopascal Air Pump

Types Of Air Pump For Biofloc-

1) Root Blower -

8
2) Diaphragm Air Pump

3) Magnetic Air Pump

4) Ring Blower

9
5) Fish Suitable for Biofloc - Biofloc
Biofloc Suitable Catfish
Pangasius, Koi, Singhi, Desi Magur, Tengra, Pabda IMC Indian
Major Carp Tilapia, Common Carp, Amur Carp, Floc
Biofloc
Catfish Biofloc

IMC Gills

6) Items for Water Preparation Biofloc

Items

1) Raw Salt ( )

10
2) CaCo3

3) Probiotic

4)

11
7) Water Parameters Biofloc

Biofloc
Biofloc Biofloc

Important Parameters For Biofloc

Parameters Minimum Maximum

Temperature 25oC 35oC
PH 6.8 Ppm 8.0 Ppm
Ammonia 0.0 Ppm 0.5 Ppm
Nitrite 0.0 Ppm 0.5 Ppm
Nitrate 0.0 Ppm 0.5 Ppm
Alkalinity 80 Ppm 120 Ppm
Salinity 0.5 Ppt 1.5 Ppt
Floc 10ML 20ML
Dissolved Oxygen (DO) 5 Ppm 10 Ppm

8) Water Testing & Control ( )

1) PH Test - PH PH Meter
Biofloc PH 6.8 ppm ppm
PH PH 7.5

12
 The Effects of pH on Warm-Water Pond Fish
pH Effects on fish
4 Acid death point
4 to 5 No reproduction
4 to 6.5 Slow growth
6.5 to 9 Desirable ranges for fish reproduction
9 to 10 Slow growth
Alkaline death point
How to Increase Biofloc PH
Calcium Carbonate Caco3
Calcium Carbonate ph
Biofloc
Calcium Carbonate Caco3
How to Decrease PH Vineger
( ) Muriatic Acid
ph
ph

13
2) Temperature
o
Biofloc C
o
C

Tank Cover
3) Ammonia Test Ammonia Test Kit

ppm

Biofloc

Sludge
Ph

14
4) Nitrite Test No2 - Nitrite Nitrite Test Kit
Nitrite
Nitrite ppm )
Nitrite Biofloc Nitrite
Nitrification Nitrite

5) Nitrate Test No3 - Nitrate Nitrite Test Kit

Nitrate Nitrite
Nitrate ppm)

15
6) Alkalinity Test - Alkalinity Alkalinity Test Kit
Biofloc Alkalinity 80 ppm ppm

7) Salinity Meter - Salinity ( ) Salinity

Meter Biofloc Salinity 0.5 ppt
ppt
Salinity Row Salt

8) Dissolved Oxygen (DO) Meter - DO ( )

DO Meter Biofloc DO 5 ppm
ppm

16
9) Water Preparation Parameters
Biofloc
water Preparation 7 Biofloc Water
Preparation
Step 1 Air Pump

Step 2 Add Row Salt -

Row Salt ( ) Aeration
Step 3 Add CaCo3 - CaCo3
CaCo3 PH PH 7.5 ppm
CaCo3 PH 7.0
CaCo3 Aeration
Step 4 Add Molasses & Probiotic - Molasses &
Probiotic Molasses & Probiotic
Probiotic molasses ( ) probiotic

Step 5 Molasses
parameters

17
 10) Floc Development

Floc Floc ML

11) Fish Sanitization & Stocking ( )- Biofloc

Sanitization

Important Point for Fish Sanitization & Stocking -----

Biofloc

Sanitization Potassium Permanganate Row salt

Potassium Permanganate

Potassium Permanganate
Biofloc
12) Ammonia Control & C:N Ratio Biofloc
Roll Biofloc
Biofloc

wastage

Sludge
PH

Ammonia TAN- Total Ammonia Nitrogen Carbon

18
C:N Ratio

C : N

Carbon Nitrogen

Molasses Or Sugar Ammonia

Pure Carbon

C:N Ratio Maintain

Carbon
10 ratio
10 : 1
C:N Ratio maintain
45

molasses
Biofloc C:N Ratio Calculation
I. Fish Feed Calculation
II. Ammonia Test Kit Easy Ammonia PPM(Part Per Million)
ppm

Gram Mile Gram .

0 0 20
Molasses, Sugar Jiggery)

19
 Waste

ratio ratio
ratio extra gm
gm
Molasses

Some Important C:N Ratio Calculation-----

Water Ammonia Pure Carbon Molasses Sugar

Liter ppm = Gram 1 Gram 45 Gram 25 Gram
Liter ppm = Gram 1 Gram 450 Gram 250 Gram
Liter ppm = Gram 2 Gram 900 Gram 500 Gram
2 Liter 2 ppm = Gram 4 Gram 1800 Gram 1000 Gram

13) Feed Management ( ) Biofloc FCR(Feed

Conversion Ratio)

FCO (Fermented Carbon Organic)

kg ml FCO ml

Feed Supplement

20
FCO (Fermented Carbon Organic) ;- FCO

Probiotic
kg Molasses

AIR Aeration Air Stone

Heterotrophic

Water Preparation Probiotic

0
0

kg total Body Weight

FCR(Feed Conversion Ratio) FCR

kg kg FCR

21
 FCR = Total Feed Total Weight
Gram / Gram
FCR

Feed Management Chart ( )

Fish Body Feed % (Total Floating Feed Protein%

Weight (Gm) Body Weight) size (MM)
1-5 gm 4% only Biofloc 0.5-0.8 mm 38-32%
6 20 gm 6% 1.0- mm 32%
21-50 gm 5-6% 1.5 mm 30%
51-100 gm 4% 2 mm 28%
101-300 gm 3.5-3% 3-4mm 28%
301-400 gm 2.5% 3-4mm (Mix) 24%
401-600 gm 1.5-2% 4mm 24%
601 gm Above 1% 4mm or 6MM (Mix) 20%

14) Daily Routine in the Fish Farm

parameter
parameter Data
C : N Ratio Maintain

disturb
Water Parameters Data Chart Format-

Date PH TDS Ammonia Temp. Nitrite Nitrate Feed Molasses

22
15) Fish Important Disease & Control ( )
I. Bacterial Diseases
II. Fungal Diseases
III. Parasitic Diseases
IV. Viral Diseases

Bacterial Diseases- Bacterial

a) Fin & Tail Root

Treatment - Potassium Permanganate 0

Tetracycline B-Complex 2-2 Capsule - gm kg

b) Dropsy

23
Treatment

TDS 2500

Oxytetracyclin kg
c) Eye Disease

Treatment Potassium Permanganate

Tetracycline B-Complex 2-2 Capsule - gm kg

d) Columnaris

Treatment Copper Sulphate Potassium Permanganate

Tetracycline B-Complex 2-2 Capsule - gm kg

24
Fungal Diseases - Fungal

a) Cotton Wool Disease

Treatment TDS 3000 PPM

Potassium Permanganate

Tetracycline B-Complex 2-2 Capsule - gm kg

b) Gill Root Disease - fungal

25
 Treatment Potassium Permanganate Copper Sulphate
1
Ciprofloxacin Tetracycline B-Complex 2-2 Capsule - gm kg

c) Ichthyophonus Disease

Treatment ,
Parameter

Antibiotic

16) Harvesting and Selling

Biofloc
Harvesting

Harvesting ,
Harvesting

26