DECLARATION

I hereby declare that the thesis entitled “ Synthesis of silver nanoparticles using combination
of Beta vulgaris and Mentha extract to check its antibacterial and catalytic activity”
submitted in partial fulfilment for the award of degree of Master of Science Microbiology to
Shoolini University of Biotechnology and Management Sciences, Solan (H.P.) is original
research work carried out by me under the guidance and supervision of Dr.Nitika Thakur. No
part of this thesis has been submitted for any other degree or diploma to this or any other
university.

Name and signature of student

Place :

Date :
 CERTIFICATE -I

This is to certify that the thesis entitled “Synthesis of silver nanoparticles using combination
of Beta vulgaris and Mentha extract to check its antibacterial and catalytic activity”
submitted in partial fulfilment for the award of the degree of Master of Science in Microbiology
to Shoolini University of Biotechnology and Management Sciences, Solan (H.P.) is original
research work carried out by NITIKA THAKUR (PGD/2021/4794)under my/our guidance and
supervision. No part of this thesis has been submitted for any other degree or diploma to this
or any other university.

The assistance and help received during the course of investigation has been acknowledged.

(Name and Signature of Major Research Guide)

Place:
Date:
 CERTIFICATE-II

The thesis entitled “ Synthesis of silver nanoparticles using combination of Beta vulgaris
and Mentha extract to check its antibacterial and catalytic activity” Submitted by NITIKA
THAKUR(PGD/2021/4794) to Shoolini University of Biotechnology and Management
Sciences, Solan (H.P.), in partial fulfilment for the award of the degree of Master of Science in
Microbiology in the Faculty of Applied Sciences and Biotechnology has been approved by the
student’s Research Guiding Committee after an oral examination of the same.

Name & Signature of Chairman

Name & Signature of Research Guiding Committee Members

Dean of the Faculty

Dean Academic Affairs

 ACKNOWLEDGMENT

First and above all I would to thank the almighty God for bestowing upon me his
choicest blessings and providing me the capability to proceed successfully in my life.
The thesis bears the imprint of many people. With immense pleasure and deep
satisfaction, I express my gratitude to Dr. Nitika Thakur, Supervisor for the M.Sc.
program, for her valuable guidance, constant inspiration, encouragement, help, and
sustained interest during thestudy, research, and preparing the thesis.

I offer my profound gratitude to Dr P.K Khosla, Chancellor of Shoolini University of

Biotechnology and Management Science Bajhol, Solan (HP) for providing me the
opportunity to do this research work in this esteemed institution. I also pay sincere
thanks to Dr. Saurabh Kulshreshtha, Dean of the School of Biotechnology Shoolini
University for providing me a chance to undergo research in this department.

Finally, an honorable mention goes to my beloved parents and my friends (Rahul,

Rashi) for their love, inseparable support, and whole-hearted blessing for the
successful completion of this project with sincerity. May almighty bless all the nice
souls mentioned above!

Nitika Thakur

(PGD20214794)
 LIST OF SYMBOLS ANS ABBREVIATIONS

S. No Symbols Abbreviation

1. g Gram

2. ml Milliliter

3. Cm Centimeter

4. % Percentage
SEM
5. Scanning electron microscopy
°C
6. Calciols
 LIST OF TABLES

Table Title Page No

No.

1. Nutritional value of beetroot 13

Numerous antiviral characteristics of mint

2. 14

Results of antimicrobial test

3. 34
 LIST OF FIGURES

Figure Title Page

No. No.

1. Raw sample of Beta vulgaris 12

2. Samples of Beet root and Mint 24

3. Aqueous extract of Beetroot 25

Mint color changes from green to brown – green

4. 25

After centrifugation dry the samples in hot air oven for two
5. days 26
According to the scanning electron microscope (SEM)
6. silver nanoparticles with mean particle size of 26 nm and 33
spherical shape were the most frequent particles

Antibacterial tests carried out on a) Escherichia coli, b)

7. Staphylococcus aureus, c) klebsiella 34