BUREAU OF F ISHERIES AND AQUATIC RESOURCES

NATIONAL FISHERIES LABORATORY DIVISION

Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

FEE PER PNS ISO/IEC

RELEASING
ANALYSIS METHOD DETECTION LIMIT REQUIREMENTS SAMPLE 17025:2017
RESULTS
(PhP) ACCREDITED
FISH HEALTH LABORATORY (FHL)
A. Residue Analysis
1. Chloramphenicol (CAP) ELISA Meat: 0.008 ppb Fresh Chilled/Frozen 1,000.00 Fifteen (15) Accredited for CAP, AOZ
working days after and AMOZ in Food and
-Whole Finfish (Milkfish, Tilapia, sample preparation Agricultural Products for
Feeds: 0.100 ppb 1,000.00
Stonefish, Eel, Grouper, Pompano the following sample
2. Nitrofurans etc.): type:
At least 1.3 kg (2-3 pcs)
a. AOZ ELISA Meat: 0.070 ppb 1,000.00 A. Fish
-Bamboo Shark: At least 1.3 kg. B. Crustaceans (Shrimps,
b. AMOZ Meat: 0.140 ppb 1,000.00 Lobsters, Sea Mantis etc.)
-Salmon Head/Belly: At least 2 kg. C. Mollusks
3. Aflatoxin Feeds: 2.00 ppb 1,000.00 D. Derived Products
-Shrimp (Head-on): At least 1.3 kg.
Using RIDASCREEN
-Sea Mantis: At least 1.2 kg. Commercial Kit

-Lobsters (Headless): At least 800 g.

(at least 2 pieces)

-Canned/Pasteurized/Chilled
Crab Meat: At least 300 g

Live
-Geoduck: At least 1.5 kg (at least 2
pcs)
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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-Crabs: At least 1.2 kg (2-3 pcs with

claws attached)

-Nylon Shell: 1.2 kg

-Other Shells: At least 1.5 kg

Kept Dry
Aquatic Feeds: At least 300 g
B. Molecular Diagnostic
1. for Crustaceans Conventional PCR Qualitative Live, chilled, frozen or FIXED in 95% 600.00 Five (5) working Accredited for the
(Shrimp, Crabs, etc.) (WSSV, IHHNV, EHP, Ethanol per analysis days after sample Identification of
AHPND, MBV, NHPB, receipt pathogens in plants and
DNA TSV, IMNV, YHV/GAV, Shrimp: At least 300 pcs for fry/pl or animals (Crustaceans)
MRNV, PVNV and at least 5 pcs adult for the following
-WSSV, IHHNV, EHP,
CMNV) microbial pathogens:
AHPND, MBV, NHPB and
Crabs: At least 2pcs
DIV 1 A. White Spot Syndrome
Real Time PCR
(WSSV, EHP, IMNV, Virus (WSSV)
RNA B. Infectious Hypodermal
TSV, YHV and
- TSV, IMNV, YHV/GAV, AHPND) and Hematopoietic
MRNV, PVNV and CMNV Necrosis Virus (IHHNV)
C. Enterocytozoon
Insulated hepatopenaei (EHP)
Isothermal PCR- D. Vibrio
IIPCR parahaemolyticus
(DIV 1) (AHPND)
E. Necrotising
Hepatopancrea Bacteria
(NHPB)
F. Monodon Biculovirus
(MBV)
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

G. Infectious Myonecrosis
Virus (IMNV)
H. Taura Virus (TSV)
I. Macrobrachium
rosenbergii nodavirus
(MRNV)
J. Yellow Head Virus
(YHV)
K. Penaeus vannamei
nodavirus (PVNV)

Using Conventional PCR

Commercial Kit
2. for Finfishes (Milkfish, Conventional PCR Qualitative -Live, Chilled, frozen or FIXED in 95% 600.00 Five (5) working N/A
Tilapia, Koi, etc.) (KHV, Irido, SCVC and Ethanol per analysis days after sample
VNN) receipt
DNA -At least 30 pcs fry/fingerlings
-KHV, Irido Insulated
Isothermal PCR- -At least 10 juvenile/ grow out
RNA IIPCR moribund or fish exhibiting
- TiLV, VNN and SVCV (TiLV and Irido-G) clinical signs of a disease
3. Parasitological Microscopic Qualitative Must be LIVE 75.00 Three (3) working N/A
Examination Finfish: At least 30 pcs days after sample
receipt
Lobster: At least 10 pcs with
carapace length >10cm

Eel: At least 30 pcs (>16 cm)

Shrimp: At least 300 pcs for fry/pl

or at least 5 pcs adult
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

4. Gross Microscopic Qualitative 100.00 Three (3) working N/A

Morphological/Necropsy days after sample
/Microscopic receipt
5. Bacterial Count Serial Dilution Quantitative Water: At least 1 L submitted 100.00
(Luminous and Vibrio) within 3-4 hours from the time of
collection

Shrimp: At least 30 pcs

fry/fingerlings and at least 10
juvenile/ grow out moribund or
fish exhibiting clinical signs of a
disease
6. Physico-Chemical (Salt, Brackish or Fresh intended for Aquaculture
a. pH Lamotte pH 0.00-14.01 ppm Water: At least 1 L submitted 30.00
meter within 3-4 hours from the time of
b. Acidity/Alkalinity Unit dose Vial 0.200 ppm collection 100 .00
c. Carbon Dioxide Titration Quantitative 100.00
d. Dissolved Oxygen Winkler 0.0-10.0 ppm 150.00
(DO)
e. Ammonia Salicylate 0-1.00 ppm 180.00
f. Nitrate/Nitrite FW: 0.05-0.80
ppm;
Colorimetric SW: 0.2510.0 180.00
ppm/
FW: 0.05-0.80 ppm
g. Phosphate Ascorbic Acid 0.00-3.00 ppm
300.00
Reduction
h. Total Hardness UDV 0-450 ppm 300.00
i. Salinity Refractometer 0-100 ppm 30.00
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

7. Bacterial Identification Analytical Profile Qualitative -At least 30 pcs LIVE N/A
for Finfish Index fry/fingerlings/ at least 10
a. API 20 Strep juvenile/ grow out moribund or
b. API Staph fish exhibiting clinical signs of a
c. API 20 E disease
d. API 20 NE
200 per Ten (10) working
- If bacterial isolates are in a
analysis per days after sample
culture media, it should be
sample receipt
transported in a refrigeration
temperature with a sealed
packaging. Pertinent details about
the sample should be complete
and will be assessed first before
the receipt of samples
8. Histopathology Histotechniques Qualitative -At least 30 pcs LIVE Fifteen (15) N/A
and microscopic fry/fingerlings/ at least 10 working days after
examination of juvenile/ grow out moribund or sample preparation
histological slides fish exhibiting clinical signs of a
disease

- At least 100 pcs LIVE fry/PL for

shrimp 500.00

- At least 10 moribund
juvenile/adult shrimp

- If samples are already fixed, it

should be properly transported
with its pertinent details attached.
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

Samples will be assessed first

before the samples are received.

FIXATIVE:
Shrimp: Davidson’s fixative

Fish: 10% Neutral buffered

formalin, 10% buffered formalin,
bouin’s solution
FISHERIES PRODUCT TESTING LABORATORY (FPTL)

1. pH Good Quality: 6.2- Fresh/Chilled/Frozen: 50.00 Seven (7) working N/A

Potentiometric 6.9 Sour/Putrid: days after sample
5.2&below Cephalopods: At least 500 g receipt
2. Water activity (Aw) Heavily Salted Fish: 75.00 Ten (10) working
0.6-0.7 Mollusks: At least 2 kg w/ shell days after sample
Novasina Aw and at least 500 g meat receipt
Measuring Value Bagoong PNS/FDA
36:2012: 0.85 Crustaceans: At least 1 kg w/
3. Sodium Chloride (NaCl) Fresh: 0.2-3% shell and at least 500 g meat 160.00
Dried Fish: 8-10%
Smoked: 3-5% Fish: At least 500 g;
Titrimetry-Volhard
Bagoong: 8-15%
Dried Fish and Fishery Products:
At least 1 kg
4. Heavy Metals
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

a. Cadmium (Cd) 0.000084 ppm A. For EU and US Export and 1,200.00 per Fifteen (15) N/A
Plant Verification sample working days after
sample preparation
Fresh/Chilled/Frozen
GFA AAS NINE (9) samples per batch or
b. Lead (Pb) 0.000025 ppm production code with 300 grams 1,200.00 per
each. sample

B. For other purposes

c. Mercury (Hg) MVU-AAS 0.0054 ug/g 1,200.00 per
Cephalopods: At least 1 kg sample

Mollusks: At least 1.5 kg w/ shell

and at least 1 kg meat
5. Histamine HPLC-FLD 0.0310 mg/kg 450.00 per
Crustaceans: At least 1.5 kg sample
w/shell and at least 1 kg meat

Fish: At least 1 kg
6. Microbial Examination
a. APC Fish & Fishery Dried Fish and Fishery Products: Ten (10) working I. Accredited for Aerobic
Product: At least 1 kg 200.00 days after sample Plate Count (APC) for
BAM AOAC, 2001 the following:
500,000 cfu/g receipt
(for meat)
b. Staphylococcus aureus Fish & Fishery Fresh/Chilled/Frozen
A. Fish and Shellfish
Product: 300.00
SMEWW (for ice products
1,000 cfu/g Cephalopods: At least 1 kg B. Fresh frozen fish and
and water)
c. Fecal Coliform for Fish: 11 mpn/g cold smoked
water and ice Mollusks: At least 2 kg w/ shell 250.00
Shellfish: and at least 1 kg meat
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

16 mpn/g C. Pre-cooked breaded

Crustaceans: At least 1.5 kg w/ fish
Water: <1.1 shell and at least 1 kg meat D. Frozen raw
mpn/100 mL crustaceans
E. Cooked, chilled and
d. E. coli Fish & Fishery Fish: At least 1 kg frozen crab meat
Product: F. Fish and shellfish
11 mpn/g Water: At least 1 L contained in a products hermetically
Shellfish: sterilized bottle jar sealed containers
16 mpn/g Live (thermally processed)
Ice: At least 1 kg 350.00
Shellfish: With Pour Plate Method
23 mpn/g Bacteriological
Analytical Manual (BAM)
Water: <1.1
mpn/100 mL
II. Accredited for
e. Heterotrophic Plate Ice and Water: <1 Heterotrophic Plate
Count (HPC) for water and cfu/mL 200.00 Count (HPC) for the
ice following:
f. Salmonella Fish & Fishery
products: 400.00 A. Potable Water
Absent in 25 g B. Source Water
g. Enterococci/Fecal C. Others Ice
Streptococci Water: <1.1
350.00 With Pour Plate Method
mpn/100 mL SMEWW

AQUATIC TOXICOLOGY LABORATORY (ATL)

 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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7.0 7.2 Master List of Analyses

1. Paralytic Shellfish ELISA 0.3 ug STX Five (5) working N/A

Toxin (PST) equivalent/100 g days after sample
600.00
receipt
RBA Quantitative
2. Amnesic Shellfish Toxin AOAC Method No. SHELLFISH 2,500.00 Seven (7) working
0.2 ug/mL
(AST) 991.26 Meat: at least 250 g. days after sample
3. Diarrhetic Shellfish Protein 3,000.00 receipt
Toxin (DST) Phosphatase 2A 63 ug/Kg With shell: at least 1 kg.
Assay
4. Brevetoxin IAEA TechDOC 1729 CRUSTACEAN (ALAMANG): at 4,500.00 Fifteen (15)
< 5 ng/g least 250 grams working days after
completion of the
5.Ciguatoxin IAEA TechDOC 1729 4,000.00 required number of
5 ng PbTX eq / g samples per filter
plate
6. Cyanide 3 - 5 pcs per sample, small sized
APHA Method No. (ornamental fish)
0.04 ppm 450 .00 Seven (7) working
4500
days after sample
1 pc per sample (< 250g ) receipt
7. Formaldehyde Spectrophotometric At least 5 pcs
Method Nash 0.60 ug/g 250.00
Reagent
NOTES and REMINDERS:

1. All samples that will be submitted for analysis must have COMPLETE INFORMATION including other documentary requirements such as Local Transport Permit
(LTP) or Auxilliary Invoice for samples that are wild caught.

2. ALL samples for other analysis MUST be placed in a plastic container, plastic bag or Ziploc bag and should be properly SEALED for each sample type. KINDLY
ensure that there are no holes in the container to avoid contamination.
 BUREAU OF F ISHERIES AND AQUATIC RESOURCES
NATIONAL FISHERIES LABORATORY DIVISION
Arcadia Bldg., 860 Quezon Avenue, Quezon City
Document No. Revision No Effectivity Date Page:
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SECTION: Sub-Section: SUB-TITLE:
7.0 7.2 Master List of Analyses

3. The SEALED samples must then be placed in a Styrofoam box or Cooler Insulated Box and ice SHOULD be packed SEPARATELY around the samples. This is NOT
REQUIRED for AQUATIC FEEDS and LIVE samples.

4. Samples for MICROBIAL EXAMINATION will be accepted every MONDAY between 8:00AM to 12:00PM in a week with five (5) working days for 20 samples
(maximum of 5 samples accepted per exporters).

5. For Physico-Chemical and Microbial Analysis or Examination, samples MUST be in a Sterilized Zipped Plastic Sealer Bag or Vacuumed Sealed Plastic Bag or Bottle
and put in a Cool Box or Container or Styrofoam Box as chilled or maintain its ice temperature.

6. Aquatic Feeds samples can be analyzed for CAP and Aflatoxin only.

7. Samples for Bacteriology (Luminous and Vibrio) will be accepted from Monday to Thursday between 8:00AM to 4:00PM.

SAMPLES THAT DO NOT COMPLY WITH THE SET REQUIREMENTS AND WITH INCOMPLETE INFORMATION WILL BE REJECTED.

FOR INQUIRIES you may contact or email us:

0919-0041280 receivingnfld.bfar@gmail.com