A

TECHINCAL REPORT

ON

STUDENT INDUSTRIAL WORK EXPERIENCE SCHEME (SIWES)

TRAINING PROGRAM

UNDERTAKEN AT

GRACELAND MEDICAL CENTRE

BENIN CITY, EDO STATE

BY

ONAGHISA AISOSA JENNIFER

LSC1806825

MCB300

SUBMITTED TO THE DEPARTMENT OF MICRBIOLOGY

FACULTY OF LIFE SCIENCES,

UNIVERSITY OF BENIN

IN PARTIAL FULFILLMENT FOR THE AWARD OF THE DEGREE

OF BACHELOR OF SCIENCE (B.SC.), EDO STATE, NIGERIA

 NOVEMBER 2022

CERTIFICATION

This is to certify that ONAGHISA AISOSA JENNIFER , with matriculation number

LSC1806825 from the department of Microbiology, Faculty Of Life Sciences, University Of

Benin has successfully undergone the STUDENT WORK EXPERIENCE SCHEME (SIWES) at

GRACELAND MEDICAL CENTRE.

Signed

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 .................................................
PROF.(MRS) F.I. Akinnibosun
Head of Department

...................................................
Dr. C.E. Oshoma
Industrial Training Coordinator

...................................................
Mrs Iyelumi Augustina
Industrial Training Supervisor

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DEDICATION

I dedicate this work to God Almighty for the opportunity to be part of this Industrial

Training program. I am grateful for the grace and empowerment made available for me to be

able to complete my industrial training (IT) without sweat and also for the good health He

granted me throughout my period of attachment.

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 ACKNOWLEDGEMENT

I thank God Almighty for the golden opportunity to do my Industrial Training at Graceland

Medical Centre and for the grace to complete it successfully.

My sincere gratitude and appreciation goes to my lovely parents and siblings for their unending

love, support, care, prayers and encouragement throughout the period of my Industrial Training.

May God bless you all, I love you guys so much!

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My heartfelt appreciation goes to the H.O.D. Prof. (Mrs) F.I. Akinnibosun and my supervisor

from the University of Benin, Dr. C.E. Oshoma of the Department of Microbiology. To my

industry-based supervisor Mrs. Iyelumi Augustina thank you for your teachings, encouragement

and for investing your time and resources in me.

I also thank the entire staff of Graceland Medical Centre for making my stay at the hospital a

memorable experience. Special thanks go to the CEO and Founder of Graceland Medical centre,

Prof. Michael Emefiele Aziken for giving me the opportunity to be able to perform my Industrial

Training in his hospital. And to the H.O.D. of the Laboratory, Miss Marian and my superior, Mr.

Obaro. Finally, my love and gratitude goes to my friend and I.T Colleague at Graceland Medical

Centre, Mustpaha Aisha for your unrelenting support, encouragement and for making my

experience a lovely and memorable one. Thank you and God bless you.

ABSTRACT

This report is a summary of the experience I acquired during my six months Student

Industrial Work Experience Scheme (SIWES) in GRACELAND MEDICAL CENTRE, Edo

State. I worked in the lab assisting the lab scientists in performing series of medical tests for

patients. I acquired special knowledge and skills in carrying out most of the medical tests which

are: Packed cell volume (PCV), Malaria parasite test (MP), syphilis test, hepatitis test, urinalysis

test, Full blood count (FBC), Blood grouping test, among others. I was opportuned to work in

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four (4) sections which are phlebotomy section, hematology/Immunohematology section,

serology section and clinical microbiology section. These sections have exposed me to

precautions, rules and regulations of the laboratory, how to diagnose patients and how the tests

are being analysed.

I also acquired knowledge in Basic Laboratory Practices, preparation of media, microbial

isolation and culture, Microscopy, Gram staining, Sterilization techniques. I was also trained in

handling and use of laboratory equipments and machineries like the incubator, refrigerator,

autoclave, centrifuge and clear idea in all operative carried out in the hospital Lab.

Most importantly, it describes the activities and my experience gained during the period of the

training, it also stated the problems encountered and also gave suggestion for improvement of the scheme.

TABLE OF CONTENTS

TITLE i
CERTIFICATION ii

DEDICATION iii

ACKNOWLEDGEMENT iv

ABSTRACT v

TABLE OF CONTENTS vi

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CHAPTER ONE: INTRODUCTION 1

1.1 Brief history and background of Graceland Medical Centre 1

1.2 Organizations chart and organogram 3

CHAPTER TWO: THE LABORATORY 4

2.1 Introduction to the laboratory 4

2.2 Safety Rules in the laboratory 4

2.3 Emergency in the laboratory 5

2.4 Hazardous Materials 6

2.5 Hazardous Equipments 6

2.6 Laboratory Equipments and their Uses. 7

CHAPTER THREE: The Laboratory Sections and Various

Tests Performed 11

3.1 The Phlebotomy Department 11

3.1.1 Identification of materials used in phlebotomy and their uses 13

3.1.2 Phlebotomy diagrams 16

3.2 Hematology and Immunohematolgy (Blood Bank) Section 16

3.2.1 Materials used in hematology 16

3.2.2 Full blood Count (FBC) 17

3.2.3 Packed Cell Volume (PCV) 18

3.2.4 Blood grouping and Genotype tests 20

3.3 Serology Section 20

3.3.1 HBsAg Test for Hepatitis, VDRL (Veneral Diseases

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 Research Laboratory) test for syphilis using the Rapid Diagnostic
test strips 22

3.3.2 Blood pregnancy and urine pregnancy test using Rapid

Diagnostic Test strip 23

3.3.3 Widal Test 24

3.3.4 Retroviral Test 26

3.4 Blood Glucose 26

3.5 Microbiology Section 28

3.5.1 Basic rules for working in the Microbiology laboratory 29

3.5.2 General Remarks 29

3.5.3 Material/apparatus used in Microbiology Section 30

3.6.1 How to prepare, sterilize and test culture media 31

3.6.2. How to dispense culture media 32

3.6.3 How to inoculate culture media 33

3.7 Common media in routine use 33

3.7.1 Disposal 35

3.7.2 Precautions taken when preparing media 35

3.8. Gram staining 36

3.9 Procedure for Autoclaving. 37

3.10 Microscopy, Culture and Sensitivity Tests 38

3.11 Urine Microscopy, Culture and Sensitivity 38
3.12 High vaginal, Urethra and Endocervical swab 39

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3.13 Antimicrobial susceptibility testing 40
3.14 Interpretation of zone sizes 41

CHAPTER FOUR: Summary, Challenges Encountered, Recommendation and

Conclusion 43

4.1 Summary 43

4.2 Challenges Encountered 44

4.3 Conclusion 44

4.4 Recommendation 44

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