Name: Precy B.

Ramada Date: November 15, 2022

Module 8 – Individual Output

Some Metabolic Activities of Bacteria

Perform the activities in the following online simulations:

Catalase test: https://learn.chm.msu.edu/vibl/vibl/Catalase/catalase_HTML5Canvas.html

Oxidase Test: https://learn.chm.msu.edu/vibl/vibl/Oxidase/oxidase_HTML5Canvas.html

Place an image of your results and fill up the table of your online simulation here:

Catalase Test

Bacteria used Catalase Result Image

Streptococcus pyogenes negative

Staphylococcus aureus positive

Interpretation/Inference:
When mixed with H2O, the isolated colony of Streptococcus pyogenes showed no bubbling while
the Staphylococcus aureus showed an immediate strong bubbling. This simply means that Streptococcus
pyogenes is a catalase-negative bacteria. These bacteria may be anaerobes or may be facultative
anaerobes that only ferment and do not respire using oxygen as a terminal electron acceptor. On the other
hand, Staphylococcus aureus is an example of a catalase-positive bacteria which means having the ability
to respire using oxygen and uses the catalase enzyme to protect them from the toxic by-products of
oxygen metabolism.

Oxidase Test

Bacteria used Oxidase Result Image

Neisseria gonorrhoeae Positive
Salmonella enteritidis Negative

Enterobacter aerogenes Negative

Interpretation/Inference:
Upon rubbing the bacteria onto the DrySlide, Neisseria gonorrhoeae turned purple-black in color
while both Salmonella enteritidis and Enterobacter aerogenes were colorless or showed no changes in
color. This means that the enzyme, cytochrome c oxidase is present in Neisseria gonorrhoeae but absent
in Salmonella enteritidis and Enterobacter aerogenes. The oxidase-positive bacteria are aerobic and can
use oxygen as a terminal electron acceptor in respiration whereas the oxidase-negative bacteria just
simply do not contain the enzyme that oxidizes the test reagent but may respire using other oxidases in
electron transport.

References:
https://learn.chm.msu.edu/vibl/vibl/Catalase/catalase_HTML5Canvas.html
https://learn.chm.msu.edu/vibl/vibl/Oxidase/oxidase_HTML5Canvas.html