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Journal of Applied Ecology - 2004 - Stander - Spoor Counts As Indices of Large Carnivore Populations The Relationship
Journal of Applied Ecology - 2004 - Stander - Spoor Counts As Indices of Large Carnivore Populations The Relationship
Applied Ecology
Spoor counts as indices of large carnivore populations]
0887\ 24\
267Ð274
the relationship between spoor frequency\ sampling effort
and true density
P[E[ STANDER
Ministry of Environment and Tourism\ c:o PO Box 5102\ Windhoek\ Namibia\ and Department of Zoology\
University of Cambridge\ Cambridge CB1 2EJ\ UK
Summary
0[ Studies of large carnivore populations and\ particularly\ reliable estimates of popu!
lation density are necessary for e}ective conservation management[ However\ these
animals are di.cult to study\ and direct methods of assessing population size and
density are often expensive and time!consuming[
1[ Indirect sampling\ by counting spoor\ could provide repeatable and inexpensive
measures of some population parameters[ The relationship between true population
density and indirect sampling results has seldom been described in large carnivore
studies[
2[ In northern Namibia the population densities of leopards\ lions and wild dogs were
measured through recognition of individuals and groups[ Spoor counts were then
conducted independently\ to assess the relationship between true density and the
distribution of spoor[
3[ Sampling e}ort\ both in terms of the number of roads and total road distance in a
sample zone\ and the intensity of sampling\ had a marked e}ect on the accuracy and
precision of spoor frequency calculations[
4[ In a homogeneous habitat\ leopard spoor were evenly spread along di}erent roads
and spoor frequency was independent of road length[ Taking into account very low
sample sizes\ the spoor density of leopards\ lions and wild dogs showed a strong linear
correlation with true density[ The slope of the regression for leopards was di}erent
to that of lions and wild dogs[
Key!words] leopard\ lion\ population estimate\ wild dog[
Journal of Applied Ecology "0887# 24\ 267Ð274
267
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
268 standing[ The true density of several large African servation status "3758 km1^ 08>29?S\ 19>29?E# is
P[E[ Stander carnivores in Namibia was determined independently inhabited by Ju:Hoansi San at a density of 9=28 people
by means of direct sampling[ Extensive spoor counts km−1[ The vegetation is dominated by forest and
of these species were then performed in the same area shrub savannah woodland "Giess 0860# with Burkea
and time frame[ The paper demonstrates the sampling africana "Harms#\ Commiphora africana "Rich#\ Ter!
characteristics of spoor counts\ and describes the minalia sericea "Burch# and Grewia spp[ as the major
relationship between spoor frequency and true density species[ Both KGR and TD fall within the 399Ð499!
in a semi!arid environment where carnivores live at mm summer rainfall isohyet with annual eva!
low density[ potransporation equalling 1799Ð2999 mm\ and where
the climate is described as warm semi!arid steppe with
temperatures ~uctuating between 4 and 25>C "van der
Methods
Merwe 0872#[ The soil\ classi_ed as arenosols\ formed
The study was carried out in the Kaudom Game hard surfaced topsoil with soft interior sands\ a sub!
Reserve and Tsumkwe District\ Namibia "Fig[ 0#[ strate where the spotting of spoor on the road network
Kaudom Game Reserve "KGR# is 2731 km1 in size was moderate to easy[
with central co!ordinates of 19>19?S and 19>29?E[ Between 0880 and 0884 the population sizes of lions
Tsumkwe District "TD#\ an area with no formal con! Panthera leo Linnaeus 0647 and wild dogs Lycaon
Þ 0887 British
Ecological Society\
Journal of Applied
Ecology\ 24\ Fig[ 0[ The Kaudom Game Reserve and Tsumkwe District in Namibia with an outlay of a 133!km1 experimental zone\ and
267Ð274 the sampling roads used for the study[
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
279 pictus Temminck 0719 in KGR and lions\ wild dogs imental zone were truly random[ Some roads "09Ð
Spoor counts as and leopards in TD were ascertained[ Population size 07)# in KGR and TD followed particular vegetation
indices of large and\ hence\ true density was determined from the anomalies[ All roads were sampled at equal frequency[
carnivore recognition and monitoring of radio!collared and The number and length of roads in each zone were
populations marked individuals and groups[ True density was esti! related to an index "penetration# of the size of the area
mated independent of spoor counts[ All animals "n "Table 0# to indicate the sampling e}ort[ {Penetration
75# were immobilized following procedures described rate| is de_ned as the sum of the combined roads in a
by Stander + Morkel "0880#\ and with a traditional sample zone "KGR\ TD or experimental zone# expre!
San bow and arrow "Stander et al[ 0885#[ Photographs ssed as a ratio of 0 km to x km1 surface area of the
were used in the recognition of 35 individual wild dogs sampling zone[ When encountered\ fresh spoor was
"Maddock + Mills 0883# and 36 lions were per! assessed for species\ group size\ age and sex[ Obser!
manently marked with an individually recognizable vations of spoor were weighted by group size\ there!
hot brand\ while immobilized with veterinary fore spoor refers\ not to a group\ but to an individual
approved anaesthetics "Orford\ Perrin + Berry 0877#[ animal[ {Spoor density| is the number of individual
By the end of 0881 all the leopards in the experimental animals| spoor per 099 km "where an individual|s
zone and −79) of lions and wild dogs in the study spoor is only counted once per day#\ and {spoor fre!
areas were marked or individually recognizable[ These quency| is the number of kilometres per spoor[
ratios of marked and known animals versus unknown The majority of data presented in this paper were
animals were maintained until the end of the study collected in the experimental zone with a focus on
period[ Eighteen leopards\ 03 lions and _ve wild dogs leopards[ All the leopards that utilized the study area
were radio!collared[ were immobilized by the San using a modi_ed tra!
Spoor counts were done throughout the study area ditional bow and arrow "Stander et al[ 0885# and
over a period of 5 months with the help of experienced radio!collared[ This was only possible because of the
San hunters[ The reliability and high accuracy of their tracking skills of the San\ in particular their ability to
tracking skills have been described in detail "Stander recognize individual animals from spoor[ The spoor
et al[ 0886a#[ Relevant to the present paper are high of new or unknown leopards were followed\ and they
probabilities of correctly identifying species were then captured and marked[ The spoor of marked
"binomial^ P 0=9^ n 036#\ sex "P 0=9^ n 58# leopards were followed frequently and the San|s
and individually known animals "P 0=9^ n 21# ability to identify individuals was con_rmed[ The fre!
from spoor[ Smallwood + Fitzhugh "0882# demon! quency of leopard spoor crossing a road was moni!
strated statistical variation in the spoor of di}erent tored[ During 5 months the 19 roads were sampled
individual cougars\ based on shape and size dis! 710 times "range 00Ð15 times each# providing a total
crepancies[ The accuracy of the San in estimating sample of 2978 km[ Only spoor made the previous
group size from spoor of lions "P 0=9^ n 28# and night was recorded and the leopard|s identity deter!
wild dogs "P 9=8884^ n 01# was equally high mined from it[ An individual|s spoor was counted only
"Stander et al[ 0886a#[ once per day[
In the present study both direct population esti! The frequency of spoor on roads was assessed as a
mates and indirect sampling were done in three zones] possible index of the true population density\ and the
KGR^ a 133!km1 experimental zone in TD^ and the sex of the animal producing the spoor was used as
remainder of TD[ The 133!km1 zone\ termed the an index of the sex composition of the population[
{experimental zone| in the centre of TD "Fig[ 0# was Sampling intensity was worked out using bootstrap
selected for homogeneity in habitat and availability analyses "Sokal + Rohlf 0884# by randomly selecting
of roads to sample spoor frequency[ The roads\ in the two samples and increasing the sample progressively
form of {cutlines|\ were made for geological pros! to 3\ 5\ 7 [ [ [ x\ with fresh means\ coe.cient of variance
pecting\ and were cut through the habitat at pre! and 84) con_dence intervals calculated each time
determined random locations and angles[ "Grieg!Smith 0846#[ The data were then plotted
Roads in all three zones were scanned daily for against the sampling e}ort "e[g[ Figs 1 and 2#[ Sam!
spoor of large carnivores[ An open vehicle was driven pling intensity was determined arbitrarily at the point
at 19 km h−0 along the roads with between two and where the coe.cient of variance reached an asymptote
four people sitting on the bumper and sides of the and did not improve markedly with an increase in
vehicle scanning for spoor[ The condition of the road sample size "Grieg!Smith 0846#[
surface\ time of day and number of observers were Data were tested for normality "KomogorovÐSmor!
recorded during each road sample[ Only early morn! nov two!sample test# and the relationship between two
ing observations\ where the road condition was {fresh| interdependent variables were _tted by ordinary least
"surface not disturbed by other vehicle tracks\ rain or squares linear regression[ Usually an independent
Þ 0887 British
wind#\ and at least one observer on the bumper and variable such as sample size or true density was expre!
Ecological Society\
Journal of Applied one on the side of the vehicle\ were used for analyses[ ssed in terms of a linear function of a dependent vari!
Ecology\ 24\ In all three zones all available roads were used to able\ coe.cient of variance or spoor frequency[ Non!
267Ð274 monitor spoor frequencies[ Only roads in the exper! parametric statistics were used when data were not
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
270 Table 0[ Area size\ sampling e}ort and population densities in three sample zones in Namibia[ Population densities were based
P[E[ Stander on direct observations of recognized individuals and groups
The sum of the distances of all the roads in the area expressed as a ratio of 0 km road]
× km1 surface area[
Results
Þ 0887 British
Ecological Society\
Journal of Applied
Ecology\ 24\ Fig[ 3[ The relationship between sampling precision\ measured by coe.cient of variance "SE as the percentage of the mean#
267Ð274 and increased sample size[
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
272
P[E[ Stander
Fig[ 4[ The relationship between true density of leopards "leopards 099 km−1# in four independent segments of the experimental
zone population "open circles# and respective spoor density counts "spoor 099 km−0#[ Dotted lines are 84) con_dence intervals[
The total population density and spoor density datum "black square#\ with 84) CI\ represents the sum of the four open circle
data points\ but was not used in the linear regression analyses[
Discussion
Successful monitoring of carnivore density or presence
from spoor counts is dependent on thorough sampling
design[ Determining the most e.cient sampling inten!
sity to meet the requirements is important and may
vary considerably in areas of di}erent carnivore
Fig[ 5[ The relationship between the "a# sex ratio "0 male] x!
density[ Habitat utilization by carnivores is an impor!
female# of leopards in the experimental zone and "b# sam!
pling precision ") coe.cient of variance#\ with increased tant variable that will in~uence spoor counts on roads[
sample size[ In the uniform habitat of the present study\ especially
the experimental zone with random sampling roads\
leopards used roads at random[ Heterogeneous habi!
tats pose more of a problem as carnivores frequent
showed similar relationships between true and spoor particular vegetation or geological types\ and spoor
density "di}erences among slopes] ANCOVA^ F 9^ frequencies on roads associated with their preferred
P 0=9#[ When combined\ spoor density for lions and habitats are higher than expected "Van Dyke\ Brocke
wild dogs appears to be a function of true density with + Shaw 0875^ Smallwood + Fitzhugh 0884#[
strong linear properties "b 2=17 2 9=13^ r1 9=87^ An increase in the number of roads\ or the total
t 02=44^ P ³ 9=90#[ Error margins\ especially along length of roads sampled to determine spoor frequency
the x!axis "true density#\ are large\ but the relationship in a particular area has an increasing e}ect on the
between spoor and true density\ nevertheless\ remains precision of the data[ In the present study desired
apparent[ levels of precision\ for leopard spoor frequency in the
The slope of the regression between spoor and true experimental zone\ were obtained when road length
density for leopards is signi_cantly di}erent to that of "penetration# was increased to a ratio of 5=4 km1 sur!
Þ 0887 British
lions and wild dogs combined "ANCOVA^ F 01=87^ face area for every kilometre of road sampled[ Once
Ecological Society\
Journal of Applied P ³ 9=94#[ This di}erence indicates that\ theoretically\ the sampling road length has been determined\ the
Ecology\ 24\ higher spoor density is expected for lions and wild sampling e}ort along those roads has further sig!
267Ð274 dogs than for leopards in the event that they all occur ni_cant e}ects on both the accuracy and precision
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
273
Spoor counts as
indices of large
carnivore
populations
Fig[ 6[ The relationship between true density "animal 099 km−1# and spoor density "spoor 099 km−0# of two separate populations
of lions and wild dogs[ Both true density and spoor density are given with 84) con_dence intervals[
of the spoor frequency estimates[ As sampling e}ort between spoor and true density[ Assuming that the
increases\ accuracy tends to stabilize and precision linear properties of this relationship are stable\ the
reaches an asymptote of marginal improvement with slope is expected to vary depending on such factors as
increased sample size[ In the present study\ such a habitat use and behaviour of species[
threshold of spoor frequency estimates was reached It is acknowledged that the sample sizes in this study
at 29 spoor samples or 0199 km sample distance[ Esti! are statistically extremely small\ but nevertheless\ it is
mating the sex ratio of leopards from spoor required argued that the data and analyses are of ecological
a slightly higher sampling e}ort to attain levels of and economic importance\ given the high costs and
precision similar to spoor frequency in the same zone[ e}ort involved[ The advantages are that this approach
An understanding of the relationship between spoor of indirect sampling of spoor frequencies is objective\
frequency and true density is of primary importance free from personal bias\ is open to scrutiny and can be
when designing an indirect measure of carnivore amended or improved as more accurate information
populations[ Spoor frequency may simply be a func! becomes available "Mentis 0879#[ The use of highly
tion of range utilization or road use\ but it is fair to skilled trackers "Stander et al[ 0886a# in this study\
assume that the extent of range and road use would especially the ability to recognize individual animals\
increase under higher densities[ For this reason spoor improved our understanding of spoor distribution\
frequency under su.cient sample sizes may well be but is not essential in the design of future monitoring
an index of true density[ By double sampling\ the techniques[ Observers with moderate tracking skills\
relationship between spoor and true density may pro! such as hunters\ would be capable of determining the
vide the means to calibrate and improve the reliability distribution of spoor along roads or transect lines[
of cheaper indirect sampling techniques "Eberhardt + This paper\ however\ does not aim to provide a tech!
Simmons 0876#[ Double sampling\ however\ is expens! nique for estimating density or monitoring of trends\
ive and time consuming\ and as a result has not been but to contribute towards an understanding of the
performed in many previous studies[ relationship between spoor counts and true density[
Data from the present study show that for leopards\
lions and wild dogs\ spoor density is a function of true
Acknowledgements
density[ These relationships are strongly linear with
intercepts at zero\ which is encouraging since it would I am most grateful to the Ju:Hoansi trackers for their
be fair\ theoretically\ to assume that zero density signi_cant contribution to this study[ Their broad eco!
would result in zero spoor density[ The slope of the logical knowledge and in!depth understanding of ani!
regression for lions appears similar to wild dogs\ but mal spoor has made this study possible[ A special
signi_cantly di}erent for leopards[ This may be the thanks to Ghau\ Tsisaba\ oma\ =ui\ Kaqece\ Young
Þ 0887 British
result of di}erential range utilization illustrated by the =ui\ Nisa\ =am and Dam[ I would also like to thank
Ecological Society\
Journal of Applied variation in daily ranges and size home ranges[ It is Dirk Heinrich and Keith Eltringham for their help
Ecology\ 24\ suggested that the slope of such regressions portray and contributions at various stages[ The Namibian
267Ð274 the ecological characteristics of the relationship Ministry of Environment and Tourism^ Department
13652664, 1998, 3, Downloaded from https://besjournals.onlinelibrary.wiley.com/doi/10.1046/j.1365-2664.1998.00313.x by INASP/HINARI - PAKISTAN, Wiley Online Library on [04/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
274 of Zoology\ University of Cambridge^ WWF "US#^ traception\ reproduction and demography of free!ranging
Etosha lions "Panthera leo#[ Journal of Zoology\ London\
P[E[ Stander Grand Canyon Spur^ Caltex\ Namibia^ INTV and
105\ 606Ð622[
Total\ Namibia are thanked for provided funding and Panwar\ H[S[ "0868# A note on the tiger census technique
institutional support[ based on pugmark tracings[ Indian Forestry "Special issue
on International Symposium on Tiger#\ February\ 69Ð66[
Schaller\ G[B[ "0861# The Serengeti Lion[ University of
Chicago Press\ Chicago[
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Eberhardt\ L[L[ + Simmons\ M[A[ "0876# Calibrating popu! estimating mountain lion Felis concolor california popu!
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worths\ London[ Freeman and Company\ New York[
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Merwe\ J[H[ van der "0872# National Atlas of South West Stander\ P[E[\ + Morkel\ P[ vdB[ "0880# Field immobilization
Africa[ National Book Printers\ Cape Town[ of lions using disassociative anaesthetics in combination
Norton\ P[M[ "0889# How many leopards< A criticism of with sedatives[ African Journal of Ecology\ 18\ 027Ð037[
Martin and de Meulenaer|s population estimates for Van Dyke\ F[G[\ Brocke\ R[H[ + Shaw\ H[G[ "0875# Use of
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Orford\ H[J[L[\ Perrin\ M[R[ + Berry\ H[H[ "0877# Con! Received 05 August 0885^ revision received 03 January 0887
Þ 0887 British
Ecological Society\
Journal of Applied
Ecology\ 24\
267Ð274