Conclusion:

The three main key findings is

 Mtt Lps induced cell death

 LPS induced nitrite production and LPS induces apoptosis

in macrophages mostly through the autocrine production of TNF-α. LPS induces

apoptosis in bone marrow-derived macrophages (BMDM). LPS-induced apoptosis
reached a plateau at about 6 hours of stimulation, whereas the production of NO by
the inducible NO-synthase (iNOS) required between 12 and 24 hours. Furthermore,
LPS-induced early apoptosis was only moderately reduced in the presence of an
inhibitor of iNOS or when using macrophages from iNOS -/-mice. In contrast, early
apoptosis was paralleled by the rapid secretion of TNF and was almost absent in
macrophages from mice deficient for one (p55) or both (p55 and p75) TNF-receptors.
During the late phase of apoptosis (12-24 hours) NO significantly contributed to the
death of macrophages even in the absence of TNF-receptor signaling. NO-mediated
cell death, but not apoptosis induced by TNF, correlated with the induction of p53
and Bax genes. Thus, LPS-induced apoptosis results from 2 independent
mechanisms: first and predominantly, through the autocrine secretion of TNF-
(early apoptotic events), and second, through the production of NO (late phase of
apoptosis). Dexamethasone is a glucocorticoid medication used to treat rheumatic
problems, a number of skin diseases, severe allergies, asthma, chronic obstructive
lung disease, croup, brain swelling, eye pain following eye surgery, and along with
antibiotics in tuberculosis. In adrenocortical insufficiency, it may be used in
combination with a mineralocorticoid medication such as fludrocortisone. The
effects of dexamethasone are frequently seen within a day and last for about three
days. Dexamethasone has been investigated in the RECOVERY trial in the treatment
of hospitalised patients with confirmed or suspected SARS-CoV-2 infection and
severe disease. The results show that dexamethasone reduce 28-day mortality
substantially among patients who received oxygen or ventilation at the time of
randomisation.