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Cholesterol liquicolor 2. With Standard
Conversion factor: 1 mg/dl = 0.02586 mmol/l
CHOD-PAP-Method A sample
with Lipid Clearing Factor (LCF) C = [STD] x  [mg/dl; mmol/l]
A [STD]
Package Sizes
[REF] 10017 4 x 30 ml Complete test kit Performance Characteristics
10019 3 x 250 ml Complete test kit Linearity
10028 4 x 100 ml Complete test kit The test is linear up to a cholesterol concentration of 750 mg/dl (19.3 mmol/l).
Dilute samples with a higher cholesterol concentration 1 + 2 with physiological
[IVD]
saline (0.9%) and repeat the determination. Multiply the result by 3.
Method Typical performance data can be found in the Verification Report, accessible
The cholesterol is determined after enzymatic hydrolysis and oxidation. The via:
indicator quinoneimine is formed from hydrogen peroxide and 4- www.human.de/data/gb/vr/su-chol.pdf
aminoantipyrine in the presence of phenol and peroxidase.
www.human-de.com/data/gb/vr/su-chol.pdf
Reaction Principle If the performance data are not accessible via internet, they can be obtained
CHE free of charge from your local distributor.
Cholesterolester + H2O  cholesterol + fatty acid
Reference ranges
CHO Adults  190 mg/dl or 5.0 mmol/l
Cholesterol + O2  cholestene-3-one + H2O2
These values are given for orientation only; each laboratory should establish its
POD own reference ranges.
2 H2O2 + 4-amino-  quinoneimine + 4 H2O
antipyrine + phenol Quality Control
All control sera with values determined by this method may be employed. We
Contents recommend to use our quality control sera HUMATROL based on animal serum
[RGT] 4 x 30 ml, 3 x 250 ml or 4 x 100 ml Enzyme reagent (R1) or our SERODOS based on human serum.
Phosphate buffer (pH 6.5) 30 mmol/l
4-Aminoantipyrine 0.3 mmol/l Automation
Phenol 5 mmol/l Proposals to apply the reagents on analysers are available on request. Each
Peroxidase ≥ 5 KU/l laboratory has to validate the application in its own responsibility.
Cholesterolesterase ≥ 150 U/l
Cholesteroloxidase ≥ 100 U/l Notes
Sodium azide 0.05 % The test is not influenced by hemoglobin values up to 500 mg/dl, bilirubin
values up to 40 mg/dl and ascorbic acid up to 8 mg/dl.
[STD] 3 ml Standard
Cholesterol see label False low Cholesterol results may possibly occur with samples from patients
Sodium azide 0.095 % treated with N-acetyl cysteine (NAC, treatment of paracetamol overdose), N-
acetyl-p-benzoquinone imine and/or Metamizole. Blood sampling should be
Reagent Preparation performed before administration of metamizol.
The [RGT]/R1 and the [STD] are ready for use.
Safety Notes
Reagent Stability [STD] Warning
The reagents are stable up to the given expiry date, even after opening, when H317 May cause an allergic skin reaction.
stored at 2...8°C. The opened reagent is stable for 2 weeks at 15...25°C.
H319 Causes serious eye irritation.
Contamination must be avoided. Do not freeze.
[RGT]/R1, [STD]
Specimen P234 Keep only in original container.
Serum, heparinised or EDTA-plasma.
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
Note: Lipemic specimens usually generate turbidity of the sample/ reagent P262 Do not get in eyes, on skin, or on clothing.
mixture which leads to falsely elevated results. The CHOLESTEROL liquicolor
P281 Use personal protective equipment as required.
test avoids these falsely elevated results through its built-in Lipid Clearing
P303+P361+P353 IF ON SKIN (or hair): Take off immediately all contaminated
Factor (LCF). The LCF clears up totally a turbidity caused by lipemic specimens.
clothing. Rinse skin with water/shower.
Assay P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes.
Wavelength: 500 nm, Hg 546 nm Remove contact lenses, if present and easy to do. Continue rinsing.
Optical path: 1 cm P337+P313 If eye irritation persists: Get medical advice/attention.
P401 Store in accordance with local/regional/national/international
Temperature: 20...25°C or 37°C
regulations.
Measurement: Against reagent blank. Only one reagent blank per series is P501 Dispose of contents/container in accordance with
required. local/regional/national/international regulations.
Pipetting Scheme References
Please use the standard included in the test kits or AUTOCAL, [REF] 13160 for 1. Thomas L., Labor und Diagnose, 8 ed. (2012)
automated procedures.
2. European Heart Journal 33, 1635-1701 (2012)
Pipette into cuvettes Reagent blank Sample or [STD]
3. Richmond W., Clin. Chem. 19, 1350 (1973)
Sample/ [STD] --- 10 μl
4. Röschlau P. et al., J. Clin. Chem. Clin. Biochem. 12, 403 (1974)
[RGT]/R1 1000 μl 1000 μl 5. Trinder P., Ann. Clin. Biochem. 6, 24 (1969)
Mix, incubate 10 min. at 20...25°C or 5 min. at 37°C. Measure the absorbance
of the sample/[STD] against the reagent blank (A) within 60 min. SU-CHOL INF 1001701 GB 12-2021-30 |
Manual calculation of the Cholesterol Concentration
1. With Factor
Wavelength C [mg/dl] C [mmol/l]
Hg 546 nm 840 x A 21.7 x A
500 nm 553 x A 14.3 x A

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