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Biochemistry A Short Course 3rd Edition Tymoczko Test Bank Download
Biochemistry A Short Course 3rd Edition Tymoczko Test Bank Download
Biochemistry A Short Course 3rd Edition Tymoczko Test Bank Download
Tymoczko
Full download at link: https://testbankpack.com/p/test-bank-for-
biochemistry-a-short-course-3rd-edition-by-tymoczko-test-bank-isbn-
1464126135-9781464126130/
Matching Questions
Use the following to answer questions 1–10:
Choose the correct answer from the list below. Not all of the answers will be used.
a) protein kinase A (PKA)
b) calmodulin
c) fatty acids
d) proto-oncogene
e) R2C2
f) R1C2
g) cAMP
h) phospholipase C
i) protein kinase C (PKC)
j) epinephrine
k) oncogene
l) small G proteins
1. ____________ The primary messenger responsible for the “fight or flight” response.
Ans: j
Section: 13.2
3. ____________ The and subunits of heterotrimeric G proteins are anchored to the cell
membrane by being covalently linked to these types of molecules.
Ans: c
Section: 13.2
4. The ____________ enzyme becomes active when bound to Ca2+ and diacylglycerol.
Ans: i
Chapter 13 Signal Transduction Pathways 2
Section: 13.2
7. ____________ A gene that leads to the transformation of susceptible cell types into cell types
with cancer-like characteristics.
Ans: k
Section: 13.6
8. The ____________ protein binds to calcium ions and serves as a Ca2+ sensor in eukaryotic cells.
Ans: b
Section: 13.5
Fill-in-the-Blank Questions
11. Protein kinase A phosphorylates serine and residues.
Ans: threonine Section: 13.2
12. is the membrane protein that catalyzes the conversion of ATP to cAMP.
Ans: Adenylate cyclase Section: 13.2
18. The binding of IP3 to the IP3 receptor results in the release of from the endoplasmic
reticulum.
Ans: calcium ions Section: 13.2
19. When activated, the insulin receptor results in the mobilization of transporters to the cell
surface.
Ans: glucose Section: 13.4
Multiple-Choice Questions
21. Most signal molecules:
A) easily diffuse through the membrane and bind to a receptor in the cytoplasm.
B) bind to membrane receptors and transmit information across a membrane without
traversing the membrane.
C) carry out functions in the nucleus after binding to a receptor in the cell membrane.
D) A and C.
E) A, B, and C.
Ans: B Section: 13.1
25. Which form of the guanyl nucleotide is bound in the unactivated state?
A) GTP
B) GDP
C) GMP
D) dGTP
E) None of the above.
Ans: B Section: 13.2
29. The enzyme responsible for induction of the phosphoinositide cascade is:
A) phospholipase C.
B) phospholipase A.
C) C-dependent protein (CDP).
D) All of the above.
E) None of the above.
Ans: A Section: 13.2
30. What are the two messenger products formed by cleavage of PIP2?
A) diacylglyercol and inositol 1,4,5-triphosphate
B) diacylglyercol and inositol 1,3,5-triphosphate
C) diacylglyercol and inositol 1,3-diphosphate
D) diacylglyercol phosphate and inositol 1,4,5-trisphosphate
E) None of the above.
Ans: A Section: 13.2
32. Cross-phosphorylation is possible when two receptor proteins with kinase domains
A) are cleaved.
B) form dimers.
C) are internalized into organelles.
D) All of the above.
E) None of the above.
Ans: B Section: 13.4
35. When insulin binds to its receptor, which of the following occurs?
A) A PIP2-dependent kinase is activated.
B) Calmodulin binds to Ca2+.
C) Sos stimulates the exchange of GTP for GDP.
D) All of the above.
E) None of the above.
Ans: A Section: 13.4
36. Which of the five steps in the generalized scheme of transduction pathways is defective in
Cushing Syndrome?
A) termination
B) release of primary messenger
C) relay of information by second messenger
D) reception of primary messenger
E) activation of effectors
Ans: E Section: 13.2
Chapter 13 Signal Transduction Pathways 6
37. That Gα subunits have intrinsic GTPase activity is important in which of the five steps in the
generalized scheme of transduction pathways?
A) termination
B) release of primary messenger
C) relay of information by second messenger
D) reception of primary messenger
E) activation of effectors
Ans: A Section: 13.2
38. The cleavage of PIP2 is important in which of the five steps in the generalized scheme of
transduction pathways?
A) termination
B) release of primary messenger
C) relay of information by second messenger
D) reception of primary messenger
E) activation of effectors
Ans: C Section: 13.2
39. 7TM proteins action is important in which of the five steps in the generalized scheme of
transduction pathways?
A) termination
B) release of primary messenger
C) relay of information by second messenger
D) reception of primary messenger
E) activation of effectors
Ans: D Section: 13.3
Short-Answer Questions
40. What are some of the common structural features of the receptors to which signal molecules
bind?
Ans: The molecule must have a signal-binding site on the extracellular side of the membrane,
and must have an intracellular domain. Binding of the ligand to the receptor must induce
change into another form (transduced) that affects the shape of the intracellular portion so
the signal can be transmitted.
Section: 13.1
42. What happens when signaling paths are not terminated properly?
Ans: The cell will not be able to respond properly to new stimuli. The errant signals may lead
to cancer, uncontrolled cell growth, or other problems.
Section: 13.1
43. How many 7TM membranes are there? What are some of their functions?
Chapter 13 Signal Transduction Pathways 7
Ans: There are estimated to be thousands of 7TM receptors. Functions include sensory signals,
physiological control, exocytosis, chemotaxis, neurotransmission, cell development and
growth, and viral infection. (See Table 13.1 of the textbook for a complete list.)
Section: 13.2
44. What is the general mechanism for signal transmission by 7TM receptors?
Ans: The receptors “snake” through a membrane, with domains extending on the extracellular
and cytoplasmic sides. A ligand binds to a site on the extracellular side, inducing a
conformation change that is detectable on the cytoplasmic side of the cell.
Section: 13.2
45 How does binding of epinephrine initiate the cAMP production? Discuss briefly in terms of
receptor structure and function.
Ans: Epinephrine binds to a 7TM receptor, which interacts with heterotrimeric G protein,
causing it to exchange GDP with GTP. The binding of GTP causes the G protein to
dissociate and the active Gα–GTP complex binds to the enzyme adenylate cyclase.
Adenylate cyclase is a large membrane-embedded protein, with two large domains
located on the inside of the cell. This interaction induces a conformational change in the
enzyme, resulting in a more catalytically active form; thus, more cAMP is made. See
Figure 13.4 of the textbook for more detail.
Section: 13.2
Section: 13.5
Tyrosine
H H
kinase
H C H C
N N
O O
Section: 13.3
52. What steps lead from the activation of a cross-phosphorylated receptor tyrosine kinase to an
activated small G protein such as Ras?
Ans: The phosphorylated receptor binds an adapter protein called Grb-2, which in turn binds
Sos. Sos stimulates the exchange of GTP for GDP in Ras.
Section: 13.3 and Figure 13.16
53. What is the difference between heterotrimeric G proteins and small G proteins?
Ans: Heterotrimeric G proteins are composed of αβγ subunits. The α subunit contains the
guanyl nucleotide binding site. Upon activation by the signal-receptor event, the GDP is
exchanged with a GTP, and the βγ subunits dissociate from the α bound with GTP, which
is the form that activates adenyl cyclase. Small G proteins, such as Ras, are single-subunit
proteins. They are activated by proteins such as Sos in the EGF signal pathway. The
activation causes the exchange of GDP for GTP to convert it into an active kinase.
Sections: 13.2 and 13.3
54. Describe the role of phosphoinositol 4,5-bisphosphate (PIP2) in insulin signal transduction.
Ans: PIP2 is one of the signal molecules in the insulin pathway. It is converted into PIP3 by
phoshoinositide 3-kinase. The binding of PIP3 activates PIP3-dependent protein kinase,
which phosphorylates and activates other kinases in the pathway.
Section: 13.4
55. Why are mutated or overexpressed receptor tyrosine kinases frequently observed in tumors?
Ans: Because some small amount of the receptor can dimerize and activate the signaling
pathway even with binding to the primary messenger, the overexpression of the receptor
increases the likelihood that a “grow and “divide” signal will be inappropriately sent to
the cell.
Section: 13.6