Professional Documents
Culture Documents
Micro Med Midterms Unit Test
Micro Med Midterms Unit Test
Micro Med Midterms Unit Test
COLLEGE OF MEDICINE
2. Which of the following bacteria has cell-wall containing mycolic acid but considered non-acid fast
bacilli?
A. Nocardia
B. Corynebacterium
C. Rhodococcus
D. Streptomyces
A. I, III, V
B. II, IV
C. I, II, IV
D. IV, V
8. Which of the following stage of the growth cycle of bacteria is the most sensitive to antibiotics in
sensitivity test?
A. Physiologic phase
B. Exponential phase
C. Plateau phase
D. Decline phase
A. TCBS
B. LJ agar
13. Susceptibility testing performed on quality control organism using a new media lot number
yielded zone of inhibitions that were too large for all antibiotics tested. The testing was repeated
using media from a previously used lot number, all zones were acceptable. Which of the
following best explains the unacceptable zone sizes?
A. The antibiotic disks were not stored with the proper dessicant
B. The depth of the media was too thick
C. The depth of the media was too thin
D.The antibiotic disks were not properly applied to the media
A. Haemophilus
B. EBC
C. Enterococcus
D. L. monocytogenes
15. According to the Kirby Bauer Susceptibility Testing, what should be done when interpreting the
zone of a motile swarming organism such as Proteus?
A. The zone outside will be measured
16. The D-zone test is used to test inducible resistance on S. aureus strains demonstrating an
initial antibiotic susceptibility profile of:
A. I, II
B. II, III
C. I, III
D. II, IV
21. A gram stained sputum shows 40-50 squamous epithelial cells per LPF, along with G(+) cocci,
many gram negative rods and many g(+) cocci in pairs using the OIO. How should the MT report the
result of this smear?
B. ReportG(+) cocci, many gram negative rods and many g(+) cocci in pairs, as well as many
squamous epithelial cells
22. Assuming that the clean-catch mistream urine was processed, which of the following is indicative
of UTI?
24. Which of the following organisms will give the appropriate positive and negative reaction for the
Quality control of the test listed?
A. Gram stain= Positive: E. coli = Negative: N. meningitidis
B. Indole= Positive: E.coli = Negative: P. vulgaris
C. Catalase= Positive: S. aureus= Negative: S. epidermidis
D. Oxidase= Positive: P. aeruginosa = Negative: E. coli
27. A Beta hemolytic, catalase positive, g(+) cocci is coagulase negative by the slide coagulase test.
Which of the following is the most appropriate action in identification of the organism?
A. Report a coagulase negative staphylococci
B. Perform Novobiocin susceptibility test
C. Reconfirm the Slide coagulase test
D. Perform a tube coagulase test
28. Which of the following test should be used to differentiate S. aureus from S. intermedius?
A. Acetoin
B. Catalase
C. Slide Coagulase
D. Novobiocin
29. From the following biochemical test: G (+); Cat (+); Coagulase (+); PYR (-); VP (+); Chromagar (+);
MSA (Yellow). What is the suspected treatment for this bacteria?
A. Oxacillin
B. Vancomycin
C. Tetracycline
D. Penicillin
30. A child presented in august at the pediatric clinic with a superficial skin infection of the neck. The large
itchy lesions were cultured and diagnosis of Impetigo was made. Biochemical Tests were perfomed
and the following are the results:
BAP: B-hemolytic
Catalase: Neg
Bacitracin Test: 11mm
PYR: Positive
SXT: Resistant
6.5% NaCl: Neg
What is the probable causative agent of the Impetigo?
A. S. aureus
B. Group A Strep
C. Group B strep
D. Enterococci
31. When streaking a throat culture on 5% Sheep Blood agar plate, stabbing the agar plate provies
enhance detection for:
A. Protein M
B. Streptolysin S
C. Streptolysin O
D. DNAse
33. Neonatal meningitis is uncommon but significant disease. Two important causes of this disease may
be somewhat difficult to differentiate on preliminary investigation. Which of the following sets of tests
provide the best differentiation of Group B strep and L. monocytogenes?
A. Gram stained smear, oxidase, optochin
B. Camp test, Hippurate Hydrolysis, Beta Hemolysis
C. Gram stained smear, catalase, motility
D.Reverse Camp, Bile esculin, H2S production
36. Abiotrophia, a formerly known as Nutritionally Variant Streptococci, will not grow on routine blood or
chocolate agars because they are deficient in:
A. Hemin
B. Pyridoxal
C. Vitamin B12
D. Protein
38. All of the following are appropriate when isolating N. gonorrhea from a genital specimen, except:
A. Transport the genital swab in charcoal transport medium
B. Plate the specimen in MTM
C. Incubate in increased CO2
D.Culture in BAP and in ambient oxygen at 37C
39. Gray, Translucent, smooth colonies, may have dry, claylike consistency on BAP:
A. N. gonorrheae
B. N. meningitidis
C. N. lactamica
D. Moraxella
E. N. elongata
40. Large, nonpigmented or gray, opaque smooth friable, hocky puck consistency; colony may be
moved intact over surface of CAP. Oxidase positive; DNAse Test: Colorless; Butyrate Test: Positive
A. N. gonorrheae
B. N. meningitidis
C. N. lactamica
D. Moraxella
41. A sputum culture from a13 year old cystic fibrosis patient grew a predominance of short, gram
negative rods that tested oxidase negative. On MacConkey, Chocolate and blood agar plates, the
organism appeared to have a lavender-green pigment and smells like ammonia. Further testing
showed:
Motility (+)
DNAse: Colorless
Glucose: Oxidizer
Lysine Decarboxylase test: +
A. S. maltophilia
B. P. aeruginosa
C. A. baumanii
D.B. cepacian
42. Agram negative rod is isolated from a patient with second and third degree burns. The isolate
produces a bluish-green pigment and a characteristic fruity odor. Other characteristic observations
are:
TSI: K/K Motility: Positive Oxidase: Positive
A. Acinetobacter
B. Pseudomonas
C. Moraxella
D.E. coli
43. Which organisms are plump coccobacilli that may resist decolorization and maybe mistaken for
Neisseria?
A. Acinetobacter
B. Bordetella
C. Stenotrophomonas
D.Burkholderia
44. Colonies appear black or very dark purple and cultures smell ammonium cyanide (almond-like)?
A. S. maltpohilia
B. C. violaceum
C. C. meningosepticum
D.S. marsescens
45. A group of elementary students became ill after eating undercooked ground beef prepared in
school cafeteria. The suspected bacteria is EBC. Panel of biochemical tests were performed.
TSI: Yellow/ Yellow with Gas Urease: Negative
VP: Negative MUG: No blue fluoresence
Indole: Positive MacConkey with Sorbitol: Colorless
Citrate: Green Color
A. E. coli
B. Klebsiella
C. Enterobacter
D.Edwardsiella
46. A sputum culture from a nursing home patient grew gram-negative rods on MacConkey agar as
pink to dark pink Oxidase Negative colonies. Given the following result, which is the most likely
organism?
TSI: Yellow/ Yellow with Gas LDC: Positive
VP: Positive Citrate: Blue Color
Indole: Positive Motility: Negative
Urease: Positive Resistant to Carbapenem
A. E. coli
B. K. pnumoniae
C. K. oxytoca
D.P. vulgaris
47. A leg culture from a hospitalized 70 y/o diabetic men grew motile, lactose negative colonies on
MacConkey agar. Given the following reactions at 24 hours, what is the most probable organism?
H2S TSI: (-) Indole: Negative
MR: Negative Citrate: Positive
DNAse: Positive Phenylalanine Deaminase: Negative
Gelatinase: Positive Lipase: Positive
A. E. coli
B. P. vulgaris
C. P. mirabilis
D. S. marcescens
48. Three blood cultures taken from a 30 y/o cancer patient receiving chemotherapy &admitted with
UTI grew a lactose-negative, motile, gram negative rods. Given the following biochemical reactions,
which is most likely organism?
LIA: R/A, H2S (-)
TSI: K/A, H2S(+)
IMViC: ++-v
Urease: Magenta
PAD: Green
A. P. vulgaris
B. P. mirabilis
C. P. retgerri
D.E. coli
49. A bloody stool cultured from 26 y/o woman after 3 days of severe diarrhea showed the following
results:
MacConkey agar: Colorless
HEA: Blue green colonies
SSA: Colorless colonies without black centers
Negative for Oxidase, Indole, Urease, H2S
Catalase: Negative
A. Shigella dysenteriae
B. Shigellasonnei
C. Salmonella typhi
D.E. coli
50. Three consecutive stool cultures from a25 year-old male patient produced scant normal fecal flora
on MacConkey agar and Hektoenagar. However, colonies on CIN agar shows bull’s eye colonies
with Oxidase negative test. The patient had been suffering from enterocolitis with fever,
diarrheaand abdominal pain for 2 days. What is the most likely identification for this gran negative
rod?
A. Y. pestis
B. Y. enterocolitica
C. Aeromonas
D.Klebsiella
51. Which of the following tests best differentiate Shigella from E. coli?
A. H2S, VP, Citrate, Urease
B. H2S, MR, Citrate, Urease
C. Lactose, Indole, ONPG, motility
D.Gas, Citrate, VP
52. Which of these enzymes are not possessed by delayed lactose fermenters?
A. β-galatosidase
B. β-galactoside permease
C. Lactose permease
53. Identify the bacteria given the following biochemical tests results:
TSI: Yellow/ Yellow G(+) LDC: +
EMB: Fish eye colonies ODC: +
Motility: Positive ADH: Negative
IMVIC: --++
A. E. cloacae
B. E. aerogenes
C. E. agglomerans
D. K. pnuemoniae
E. P. shiggeloides
54. A culture of decubitus ulcer show agram-negative facultative bacilli. On TSI, it produced an
alkaline slant and acid slant with blackening of the agar. Test reaction in other media were as
follows:
Indole: Negative Urease: Negative after 1 hr PAD: Negative Citrate: Positive
A. P. mirabilis
B. C. freundii
C. C. koseri
D. P. vulgaris
55. An isolate of E. coli recovered from the stool of a patient with severe bloody diarrhea should be
tested for which sugar before sending to a reference laboratory?
A. Sorbitol
B. Lactose
C. Sucrose
D. Mannitol
56. The positive result on Citrate test is the alkalinity of the medium after the bacteria had utilized
the citrate. The positive color is:
A. Blue
B. Green
C. Yellow
D. Red
57. Which of the following tests detects the production of mixed acids as a result subsequent
metabolism to pyruvate?
A. Methyl Red
C. Citrate
D. Indole
58. Tryptophan broth is inoculated and incubated 24 hours. After incubation, Kovac’s reagent is
added. A red color develops at the surface of the broth. What product of metabolism is formed?
A. Mixed acids
B. Acetoin
C. Malonate
D. Indole
60. Which organism is associated with Hemolytic Uremic Syndrome & TTP?
A. E. coli 086
B. E. coli 0157:H7
C. EIEC
D. EPEC
B. Taxo N &Dimethylsulfoxide
D. Sulfanilicacid &a-naphtylamine
62. Enterobactericeaeare:
A. All are lactose fermenters but not all are glucose fermenters
B. Facultative aerobes
A. O.5% NaCl
B. O.5% Desoxycholate
C. 0.5% ethanol
D. 2% acid alcohol
64. A 35 y/o patient is diagnosed with Guillain-Barr Syndrome & gastroenteritis. Isolate shows
growth on Campy and Skirrows Medium incubated at 42C. Further biochemical tests were
performed. There was a purple color on Hippurate hydrolysis test. From these results, what is
causative agent?
A. C. coli
B. C. jejuni
C. C. fetus
D. H. pylori
65. H. pylori can be identified presumptively by the following positive tests:
67. A small gram-negative coccobacillus from CSF of a2 year old child have the following results:
Indole: Positive Glucose= (+ acid) Hemolysis: (-)
X requirement: + V-requirement: +
Urease: +/- Porphyrin Test: Negative
A. H. ducreyi
B. H. aphrophilus
C. H. influenzae
D. H. parahaemolyticus
68. A mixture of slender gram negative rods an coccobacilli with rounded ends was recovered from a
blood culture from a patient following root canal surgery. Given the following results, after 48
hours, what is the most likely organism?
Negative = Cat; LDC; Urease; ODC; X & V requirement; Indole
Positive= Oxidase, Piiting of agar in MacConckeyagar
A. H. aprophilus
B. A. actinomycetemcommitans
C. C. hominis
D. E. corrodens
E. K. kinggae
69. Smooth gray colonies showing no hemolysis are recovered from an infected cat scratch on blood
and chocolate agar but fail to grow on MacConkey agar. The organisms are gram-negative
pleomorphic rods that are both catalase & oxidase positive and strongly indole positive. The most
likely organism is:
A. Chromobacterium
B. Capnocytophaga
C. Pasteurella
D. Kingella
72. A newborn girl becomes febrile and will not feed about an hour after birth. A gram (+) is recovered
from blood cultures from the newborn. The isolate has the following biochemical tests:
BAP: Beta hemolytic Catalase: Positive BEA: Positive
H2S : Negative Motile at room temp Hippurate: Purple
A. L. monocytogenes
B. Corynebacterium
C. Group B strep
D. Erysopelathrix
73. The production of H2S is one of the characteristic used to differentiate which of the aerobic g (+)
bacilli?
A. Listeria
B. Erysopelathrix
C. Corynebacterium
D. Lactobacillus
B. II, IV, VI
C. I, II, III
D. IV, V, VI
76. Which of the following organisms will display absence of opalescence in Nagler Test?
I. C. perfringens
II. C. botulinum
III. C. sordelli
IV. C. novyia
V. C. bifermentans
VI. C. sporogenes
VII. C. baratti
B. II, IV, VI
C. I, II, III
D. IV, V, VI
77. A slow-growing Mycobacterium that typically produces a rough buff-colored colony that is non-
chromogenic in a light test:
A. M. kansasii
B. M. scrofulaceum
C. M. tuberculosis
D. M. leprae
B. Nalidixic acid
C. N-acetlyl-L-cysteine
D. Malachite green
79. Chistensen’s urea agar slants contain glucose, urea, NaCl, agar and phenol red. The
development of pink-red color is the result of:
80. Oxidative-fermentative (OF) medium of Hugh and Leifson is well suited for nonfermenters
because of a:
81. A TSI tube inoculated with an organism gave the following reactions: I. Alkaline slant II. Acid butt III.
H2S positive IV. Gas produced. The organism is most likely:
A. K. pneumoniae
B. S. dysenteriae
C. S. typhimurium
D. E. coli
82. A TSI tube inoculated with an organism gave the following reactions: I. Alkaline slant II. Acid butt III.
No H2S IV. No gas produced. The organism is most likely:
A. K. pneumoniae
B. S. dysenteriae
C. S. typhimurium
D. E. coli
B. Iridescent, brown
84. A pink zone on a DNase plate containing toluidine blue would be seen in 24 hours around colonies of:
A. Enterococcus faecalis
B. Serratia marcescens
C. Escherichia coli
D. Staphylococcus epidermidis
85. What is this organism that exhibits a safety-pin appearance that grows colonies with deep pink
color in Ashdown media?
A. B. pseudomallei
B. Y. pestis
C. P. aeruginosa
D. S. marcescence
Part 2. Application:
1. Give the functions/importance/ purposes of:
a. Biochemical Testing of bacteria
- Microbial biochemistry tests shorten the time required to identify microbes, reduce costs,
and ensure or enhance the accuracy of identification of an unknown sample. It is the
fastest developing trend in microbial identification. In recent years, the rapid commercial
test kits for anaerobic bacteria have become available.
- The structural differences with respect to shape, size and arrangement of bacteria only
help in the process of identification, because there are many species of bacteria having
similar shape, size and arrangement. Therefore, ultimately, the identification of bacteria is
mostly based on the differences in their biochemical activities. Biochemical tests are
among the most important methods for microbial identification. It shortens the time
required to identify microbes, reduce costs, and ensure or enhance the accuracy of
identification of an unknown sample.
-
b. Antibiogram
- Antibiograms help guide the clinician and pharmacist in selecting the best empiric
antimicrobial treatment in the event of pending microbiology culture and susceptibility
results. They are also useful tools for detecting and monitoring trends in antimicrobial
resistance. When antimicrobial susceptibility testing data are summarized cumulatively
for a hospital, healthcare system, or other healthcare facility periodically (such as yearly
or quarterly), trends in resistance can be identified and investigated.
2. Give the IMViC reactions of the following enterobacteria:
a. Yersinia intermedia (-+--)
b. Yersinia enterolitica (+/-,+,negative after 24 hours at 36°C but positive after
48h at 25°C, + (v+--)
c. Edwardsiella tarda (++--)
d. Proviencia stuartii (++-+)
e. Providencia rettgeri (++-+)
f. Proteus mirabilis( -+-+)
g. Proteus penneri (-+--_
h. Citrobacter freundii (-+-+)
i. Serratia liquefaciens (--++)
j. Hafnia alvei (-vv-) (v-variable)
k. Enterobacter agglomerans (-,-,++)
l. Enterococcus cloacae= (--++)
m. Klebsiella oxytoca (+,+,++
n. Klebsiella pneumoniae(-,-++)