1.

OBJECTIVE:
The purpose of this document is to provide a procedure for identification of drug actives and
Excipients or any other substances through software.
2. SCOPE:
This SOP covers the procedure of proper handling of FTIR in order to identify different samples to
perform an accurate identification and qualification.
RESPONSIBILITIES:
1. Lab Manager
2. Service Engineer.

3. Principle of FTIR:

FTIR relies on the fact that the most molecules absorb light in the infra-red region of the
electromagnetic spectrum. This absorption corresponds specifically to the bonds present in the
molecule. The frequency range is measured as wave numbers typically over the range 4000 –
600 cm-1.
FTIR spectrometer consists of following components
Source, interferometer, sample compartment, detector, amplifier, A/D convertor, and a computer.
The source generates radiation which passes the sample through the interferometer and reaches
the detector.
3 Precautions
 Maintain the temperature in the work space between 16 to 27 C.
 Avoid placing the instrument near air-conditioning duct or large windows and also protect
from heating source.
 Protect from dust particles and clean daily.
 To keep spectrometer in good order, check the desiccant indicator frequently and
replace the desiccant canisters as soon as they become saturated (pink color).
Care should be taken while taken volatile liquid spectra; there is no need to apply pressure for
liquid samples.
3. PROCEDURE:

a- Clean the crystal and knob with dry tissue then clean with pure methanol.
b- Plug-in and turn it on the instrument and PC.
c- Log-in by using ID and Password.
d- Click on Ominc software on PC.
e- Press the option “COL Background” on the top.
f- Place the sample to be analyzed on the crystal by using butter paper.
g- Fix the knob on crystal and apply the pressure until a click sound is heard.
 h- Press the option “COL SAMP” in the software on the top.
i- Enter the sample name to be analyzed.
j- Go to the option “Analyze” on the task bar and select Library Set-up then select
appropriate library name, add it and remove the other libraries.
k- Press the option “OK” then search; spectrum will appear on the screen.
l- Interpret the result by observing the Spectra by comparing with already saved spectra in
library.
m- Take the print-out of spectra.
n- Remove the sample from its crystal carefully and clean with methanol.

REFERENCES:
Manufacture manual.
7. REFERED DOCUMENTS:
System generate report. / Performance verification.
User’s Log Book