[ABSTRACTS—-FIRST INTERNATIONAL CONFERENCE 287 [COSEGREGATION BETWEEN D2i S11 EcoRT ALLELES AND THE PAD (FAMILIAL ALZHEIMER'S DISEASE) GENE IN A PEDIGREE IF. DAVID and G. LUCOTTE, Laboratory of Molecular Genetics, INTS, 6 rue Alexandre Cabanel, Paris 15éme, France Segregation study of Alzheimer's disease (AD) gene and a cloned DNA probe (D21 S11), which detects an BooRT restriction fragment length polymorphism ( RFLP: for a sequence located in the medial part (in situ hybridization experiments jare presented) of the long arm of chromosome 21, are reported in a large cala~ lorian pedigree (Italian and french branchs} in which AD is transmitted as an lautosomal dominant mendelian trait. In this pedigree, the AD gene cosegregates with one of the alternative Jalleles at the probe (B = 1.9 EcoRI kb allele), raising the possibility of usage lof such a marker for presymptomatic diagnosis of the fourth and five's genera~ Jtions pedigree individuals at -risk for the disease. calculated lod-score, using an age-~dependant model, are : & | 0 0.05 0.10 0-15 0.20025 0.30 Ppa i | Oe Oe Ce Lod-score is low, at @= 0, but70. It is the first tine that we obtained Jan evidence of cosegregation in this family, between a polymorphic DNA marker Jand the FAD gene. In the past incompatibilities in cosegregations.was noted lwith other markers of the chromosome 21, like : D21 83, ETS2, SOD-1 and € -amyloid probes.