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Rapid - Test - Kits para Antibióticos Incluyendo Beta Star
Rapid - Test - Kits para Antibióticos Incluyendo Beta Star
Rapid - Test - Kits para Antibióticos Incluyendo Beta Star
June 2002
1
CONTENTS
Page
DISCLAIMER 3
1. INTRODUCTION 4
4. CONCLUSIONS 18
5. REFERENCES 19
2
DISCLAIMER
The Food Safety Authority of Ireland cannot accept any liability for inaccuracies in the
test specifications which were based on information provided by the manufacturers.
The costs outlines were accurate at the time of publication.
The work reported does not imply that the Food Safety Authority of Ireland endorse
or in any other way promote the products and manufacturers featured.
3
1. INTRODUCTION
Antibiotic contamination in milk can seriously affect consumers’ health by causing
allergic reactions to residues or by the development of resistant strains of
microorganisms. Therefore, subsequently antibiotic contamination in milk can also
cause significant economic losses for producers and manufacturers of milk and milk
products.
Presently, the standard test method used for the detection of antibiotic residues in
liquid milk at the pasteurising plants in Ireland is the Delvo® SP (Fallon et al.,
1995/1996). The Delvo® SP is a test dependent on rapid growth and acid production
of Bacillus stearothermophilus var. calidolactis. This test method requires a lengthy
incubation time of 2 hours and 30 minutes. Thus, to avoid delays at milk intake
points, rapid antibiotic screening tests are regularly used on raw milk at the milk
pasteurising plants prior to completion of the Delvo® SP test.
The Delvo® SP is a microbial inhibition assay. Microbial inhibition assays test a broad
spectrum of antibiotics, whereas rapid test kits generally only detect a specific family
of antibiotics.
4
inspections representative samples of the day’s production are collected for analysis
in the Department of Agriculture, Food and Rural Development Dairy Science
laboratories (Fallon et al., 1995/1996). The standard sampling protocol is 1 litre of
product sampled every 20 minutes (personal communication, Regional Veterinary
Laboratory, Sligo). The Delvo® SP test method is used by the DAFRD laboratories
for the determination of antibiotic residues in milk.
The Delvo® SP microbial inhibition test is required by DAFRD to be carried out for the
detection of antibiotics and sulphonamides in milk. However, rapid test kits are often
used by industry prior to the Delvo® SP test method, often for the positive release of
milk tankers.
The objective of the report is to provide an overview of the rapid test kits available for
the detection of antibiotics and sulphonamides in milk, thus enabling the dairy
industry to choose the test kit which is most appropriate to their use.
The report reviews test parameters such as the test sensitivity concerning the
maximum residue limits which have been set in accordance with EU Council
Regulation No. 2377/90 and amendments.
The MRL is based on the type and amount of residue considered to be without any
toxicological hazard for human health as expressed by the acceptable daily intake
(ADI), or on the basis of a temporary ADI that utilises an additional safety factor. It
also takes into account other relevant public health risks as well as food technology
aspects.
The report also includes descriptions of the various test principles and the cost
(where supplied by the relevant company) of the rapid detection kits. Microbial
inhibition tests methods based on the EU reference method for the detection of
antibiotic residues in milk are described. The minimum reported sensitivities of the
individual rapid test kits for detection of antibiotic residues in milk are compared to
both the sensitivity of microbial inhibition tests (Delvo® SP) and to the EU MRL.
5
2. MICROBIAL INHIBITION ASSAYS FOR THE DETECTION OF
ANTIBIOTICS IN MILK
Benzylpenicillin 2 6
Ampicillin 2 5
Cloxacillin 15 35
Nafcillin 6 11
Tetracycline 100 400
Oxytetracycline 200 450
Chlortetracycline 150 500
Chloramphenicol 7000 15000
Dihydrostreptomycin 4000 13000
Neomycine 1000 22000
Kanamycin 9000 28000
Bacitracin 60 140
Erythromycin 1000 2250
Rifamycin 10 140
Diaphenylsulfone 10 100
Sulphamethazine (Sulphadimidine) 500 1000
(1)
Benzylpenicillin and bacitracin expressed as IU/kg, all others antibacterial substances as
µg/kg.
6
Table 2. List of microbial inhibition assays for the detection of antibiotics in milk.
Name of Microbial Manufacturer Time per test Antibiotic tested Reaction type
Inhibition Assays
Delvotest SP Kit Gist-brocades/DSM 2. h 30 min Broad spectrum Colour change
Copan test (CH ATK) Copan Italia SpA 2 h 30 min -3 h Broad spectrum Colour change
Charm Farm Test Charm Sciences 2 h 30 min -3 hr Broad spectrum Colour change
Charm AIM-96 Charm Sciences 4 hrs Broad spectrum Colour change
*For best sensitivity a control time reading is advised using a negative control
sample.
7
The Public Health Unit of the DAFRD, Regional Veterinary Laboratory (RVL), Sligo
examine all biochemical and microbiological test methods carried out at the liquid
milk processing plants. The Public Health Unit of the DAFRD, RVL, Sligo make the
following recommendations regarding the Delvo® SP test methodology:
• The sample incubation period is crucial to the accuracy of the Delvo® SP test
method. This particularly applies to the detection of sulphonamides as the
sensitivity to sulphonamides is greatly reduced by an increased incubation time.
An incubation time of 2 hr 30 min (at the time the negative control has been
changed to yellow) is recommended with a 15 minute extension time of the test
in the case of a suspect sample. For reading times of 2 h 45 min or 3 hours the
sensitivity of the test will diminish. The use of antibiotic free skim milk powder as
a control is also advised.
• It is essential that the correct temperature is maintained in the waterbath (64°C ±
0.5°C)
• The use of a proper waterbath lid, a sloping lid is advised
• The temperature control in the waterbath should be a digital temperature readout
• Good circulation should be maintained in the waterbath
The DSM, Delvo range of products is supplied by The Carbon Group, Ringaskiddy,
Co. Cork (Contact: Gerry O’Brien 021-4378988). The cost of the Delvo® SP test is: ~
€ 96.5 per 480 tests i.e., ~20 cent per test (standard flat price). It must be noted that
the above price quotation is for the materials only cost per test.
As for the Delvo® SP test, no instrumentation is required for this test method and an
accurate, controlled circulation waterbath is essential to ensure a constant
temperature of 64°C.
8
The Copan Test is a product of Copan Italia SpA, via Perotti 10, 25125 Brescia, Italy
(phone: +39 030 2687201; +39 030 2687213; E-mail info@copanitalia.com). Davidson
and Hardy, 8 Pembroke Road, Dublin 4 (01-6600725) are the agents for the Copan
Test in Ireland. Pricing is subject to quantity discounts and volume usage.
The Charm AIM-96 Test is designed for high volume, broad spectrum screening of
raw, pasteurised, homogenised or skim milk. The results can be read by visual colour
comparison or optionally with a microplate reader. The Charm AIM-96 detects beta-
lactams, sulphonamides , tetracyclines, aminoglycosides and macrolides. 96 tests
can be completed simultaneously in approximately 4 hours. The manufacturer’s
declared sensitivity to antimicrobials using the Charm AIM-96 are outlined in Table 3.
9
Table 3. Comparison of the EU maximum residue limits (EEC Regulation 2377/90 and
amendments) with the sensitivities of the microbial inhibition assays for the detection of
antibiotics in milk.
β-lactams
Penicillin 4 2 1-2 4 3
Penethamat 4 (2) 4
Ampicillin 4 2-3 <2 6 4
Amoxicillin 4 2 2-4 4 4
Nafcillin 30 5 5-10
Cloxacillin 30 15 10-15 50
Dicloxacillin 30 10 10-15 30
Oxacillin 30 5 5-10 30
Cefecetril 125 20
Cefalexin 100 40-60 >45
Cefalonium 10 5-10
Ceftiofur(4) 100(5) 50 50-100 50 50
Cephapirin 10 5 2.5-5 10
Cephazolin 50 (10) 5-10
Cefquinom 20 (100) 30-100(8) 20
Cefaperazon 40 25-50
Tetracyclines
Chlortetracycline(4) 100(6) 150 250-500 150
Doxycycline(4) 0 (100) 150
Oxytetracycline(4) 100(6) 100 250-500 150 100-150
Tetracycline(4) 100(6) 100 250-500 100
Sulphonamides
Sulphadimidine 100(7) equal to sulfamethazine
Sulphadimethoxine 100(7) 50 50-100 15
Sulphamethazine 100(7) 25 100-200 10 50-100
Sulphathiazole 100(7) 50 50-100 5
Sulphadiazine 100(7) 50 50-100 5
Sulphadioxine (100) 100-200
Sulphamethoxazole (50) <50
Sulphamonometossina <50
Macrolides
Erythromycin 40 50 >200 100
Pirlimycin 150 (50-200)
Spiramycin 200 200 >2000 500
Tilmicosin 50 (100) 75-100 180
Tylosin 50 50 50-100 50 40-50
Aminoglycosides
Gentamicin 100 100-300 100-500 30 300-500
Neomycin 500 100-200 500-2000 100
Spectinomycin 200 (2500) >300
DH/Streptomycin 200 300-500 <1000 600/500
Kanamycin 2500
10
Antibiotic EU Maximum Sensitivity Sensitivity Charm Charm
Residue Limit(1) Delvo SP(2)* Copan(3)* AIM-96* Farm Test*
in Milk Test
µg/kg)
(µ µg/kg)
(µ µg/kg)
(µ µg/kg)
(µ µg/kg)
(µ
Quinolones
Enrofloxacin 100 (1000-1500)
Danofloxacin 30 (close to enrofloxacin)
Difloxacin 0 (close to enrofloxacin)
Flumequin 0 (no data available)
Marbofloxacin 75 (close to enrofloxacin)
Various
Bacitracin 150 (1000)
Baquiloprim 30 (60)
Chloramphenicol 0 2500 5000
Clavulanic acid 200 (not antibacterial)
Colistin 50 (no data available)
Dapsone 0 1 2-4 5
Lincomycin 150 100
Novobiocin 50 (1000)
Rifaximin 60 (50)
Thiamphenicol 50 (close to >100
chloramphenicol)
Trimethoprim 50 50 100-150 200
* It is important to note that the sensitivities of the individual test kits are based on
manufacturer’s declared levels.
(1)
Council Regulation (EEC) No. 2377/90 and amendments.
(2)
Values are for detection levels at control time. For reading times of 2 h 45 min or 3 hours
the sensitivity will diminish. Values in parenthesis are given as indication from the DSM
data-base.
(3)
Screening test, Bundesanstalt f r Milchforschung, September 2000.
(4)
Mother compound.
(5)
Mother compound and metabolites.
(6)
Mother compound and 4-epimer.
(7)
Sum of substances if this group
(8)
The lowest tested concentration (100 µg/kg) was evaluated as positive.
11
3. RAPID TEST KITS FOR THE DETECTION OF ANTIBIOTICS IN
MILK
The following section of the report and Table 4 outline the test methods available for
the rapid detection of antibiotics in milk as distinct from section 2 above which
describes the microbial inhibition assays for the detection of antibiotics in milk.
Microbial inhibition assays are lengthy and test for a broad spectrum of antibiotics,
whereas rapid test kits generally only detect a specific family of antibiotics.
The standard test method used for the detection of antibiotic residues in liquid milk at
the pasteurising plants in Ireland is the Delvo® SP which is a microbial inhibition
assay. To avoid delays at milk intake points, rapid antibiotic screening tests are often
used on raw milk prior to completion of the Delvo® SP test.
Name of Rapid Test Manufacturer Time per test Antibiotic tested Reaction type
Parallux β-Lactam Idexx Inc. 4 min β-lactams Fluorescence
Parallux Tetracyclines Idexx Inc. 4 min Tetracyclines Fluorescence
Parallux Sulphonamides Idexx Inc. 4 min Sulphonamides Fluorescence
SNAP β-Lactam Idexx Inc. < 10 min β-lactams Colour change
SNAP Tetracyclines Idexx Inc. < 10 min Tetracyclines Colour change
Charm MRL β-Lactam Charm Sciences < 9 min β-lactams Colour change
Charm MRL Charm Sciences < 9 min Tetracyclines Colour change
Tetracycline
Charm MRL Charm Sciences < 9 min Sulphonamides Colour change
Sulphonamides
Charm MRL Quinolone Charm Sciences < 9 min Quinolone Colour change
Beta s.t.a.r. UCB-Bioproducts 5 min β-lactams Colour change
Delvo-X-Press βL-II Gist-brocades/DSM < 7 min β-lactams Colour change
Fluorophos BetaScreen Advanced Instruments 10 min β-lactams Fluorescence
Test (EU) Inc.
12
test. The Parallux Test Kit identifies the specific residue detected. The minimum
sensitivities for detection of beta-lactams, sulphonamides and tetracyclines in milk
using the Parallux test kits are shown in Table 6.
Table 5. Parallux assay cartridges for the detection of antibiotic residues in milk*.
Assay Detects
BETA-LACTAM ASSAY β-Lactam drugs
BETA-LACTAM 2 X ASSAY Batch two samples for β-Lactam drugs
BL/SULFA/TETRA ASSAY B-Lactam, sulfonamides and tetracyclines
SULFA/TETRA ASSAY Sulfamethazine, sulfathiazole, sulfadimethoxine and
tetracyclines
BETA-LACTAM/TETRACYCLINE β-Lactam & tetracyclines
BETA-LACTAM/ SULFA β-Lactam & sulphonamides
CILLINS ASSAY Amoxicillin, ampicillin, cloxacillin & penicillin G
CEPHAPIRIN ASSAY Cephapirin
CEFTIOFUR ASSAY Ceftiofur
CLOXACILLIN ASSAY Cloxacillin
*Table 5 is based on manufacturer’s declared detection
The assay system was also demonstrated to be specific and sensitive for the
detection of incurred residues at FDA tolerance levels of antibiotic (penicillin-G, 5
ppb; ampicillin, 10 ppb; amoxicillin, 10 ppb; cloxacillin, 10 ppb; cephapirin, 20 ppb,
and ceftiofur, 50ppb). The Association of Official Analytical Chemists (AOAC) also
demonstrated that there was no interference in detection of minimum sensitivity
levels of antibiotic by the presence of somatic cells at approximately one million cells
per ml. Milk containing 300,000 cells/ml of bacteria commonly found in mastitic milk
was also tested, and no interference was found when testing milk containing
tolerance levels of antibiotic. There was no detectable interference of the method by
a wide variety of non-beta-lactam drugs (AOAC Performance Test 000402).
The Parallux test Kits are used as milk tanker positive release tests throughout the
UK and Northern Ireland. In the Republic of Ireland, a number of companies have
used Parallux as a screening test on milk at intake.
IDEXX, the manufacturers of the Parallux test kit have stated that the Parallux
test is only for use in a laboratory situation as it is an instrument based test which
requires trained laboratory staff. The UK dairies that use the Parallux test as a
positive release for tankers carry out the test under laboratory conditions.
IDEXX also state that they work in consultation directly with the customer to ensure
that they have the correct test kit for their requirements. In a situation where
production staff may be carrying out the testing, IDEXX will always promote the
SNAP test procedure due to ease of use.
13
3.1.2 Cost per test
The Parallux range of products are supplied in the Republic of Ireland by IDEXX
Laboratories, (Contact Sue Plant 0044 7970 245 068 / 0044 161 881 9184 or sue-
plant@idexx.com).
Equipment required:
(a) Parallux Processor for Beta Lactams, Sulfonamides and Tetracyclines. €5,680.00
(c) Beta Lactam (100) Test Kits: €400.00 (€4.00 per test)
Beta Lactam 2x (200) Test Kits: €720.00 (€3.60 per test)
Beta Lactam / Sulfonamide (100) Test Kits: €440.00 (€4.40 per test)
Beta Lactam/Tetracycline/Sulfonamide (100) Test Kits: €570.00 (€5.70 per test)
for all 3 drug families
The SNAP® test is a more user friendly test kit than the Parallux kit and is used by
some farmers to test milk at farm level.
The SNAP® range of products are supplied in the Republic of Ireland by IDEXX
Laboratories, (Contact Sue Plant 0044 7970 245 068 / 0044 161 881 9184 or sue-
plant@idexx.com
Equipment:
(b) Beta Lactam Test Kits (20): €72.00 (€3.60 per test),
€60.00 (€3.00 per test) for volumes > 15,000 tests p.a.
Tetracycline Test Kits (20): €72.00 (€3.60 per test)
14
3.3 CHARM SCIENCES INC., CHARM MRL ANTIBIOTIC TEST KIT.
The Charm MRL antibiotic residue test is a rapid test kit for the determination of beta-
lactams, sulphonamides, tetracyclines and quinolones in bovine milk. There are
separate strips for testing each of the aforementioned antibiotic families. The
minimum sensitivities for detection of the beta-lactams are shown in Table 6. The
Charm MRL antibiotic residue test takes < 9 minutes to complete. The Charm MRL
test can be used for raw commingled bovine milk, individual cows milk and
homogenised whole milk (not for skim HTST milk or UHT milk).
The Charm ROSA Reader can store up to 4,000 results and you can also download
the results from the reader onto a PC database (using Microsoft Excel) or to a printer.
The MRL test strips can be frozen for three months.
The Charm MRL Beta-lactam test is used in many milk plants as a positive release
for tankers. The Charm MRL Beta-lactam kit is the only one of the Charm antibiotic
residue rapid test kits in use presently in Ireland by dairy companies.
An advantage of the Charm MRL is that there are no moving parts in the kit.
Equipment Required:
(a) The Charm ROSA Reader for beta-lactams, sulphonamides, tetracyclines,
quinolones and aflatoxin, each €6031.26.
(b) Dual place Incubator, each €612.00 /Quad Incubator, each €747.90.
The above kits quotes are for milk and the cost of the equipment and consumables
depends on the number of tests in a given workload. Charm MRL is also used for
testing tissue and urine samples, however, these kits are more expensive.
15
3.4 UCB-BIOPRODUCTS BETA S.T.A.R. ANTIBIOTIC TEST KIT.
Beta s.t.a.r. is a receptor assay for rapid determination of beta-lactams antibiotics in
milk. The Beta s.t.a.r. antibiotic test kit supersedes the PENZYME® antibiotic test kit.
The minimum sensitivities for detection of the beta-lactams using the Beta s.t.a.r.
Test Kit are shown in Table 6.
The test involves a specific Beta-lactam receptor linked to gold particles. The
preliminary incubation of a specific amount of the receptor with milk containing
antibiotics results in the interaction of the antibiotics present with the receptor. In the
second stage the solution is transferred onto an immunochromatographic medium.
The first band of this medium will capture all the receptors which have not seen any
antibiotic during the first incubation. The second band on the
immunochromatographic medium serves as a reference band.
The Beta s.t.a.r. assay takes 5 minutes to complete and is used for rapid
determination of beta-lactams in milk.
The milk samples and reagents are mixed in tubes coated with a β-lactam receptor.
After washing the tube, a colour developer is added that detects residual binding
sites not occupied by antibiotic present in the milk sample. Colour development is
inversely proportional to the concentration of β-lactam residues in the sample. Six
samples and a standard can be evaluated at a time and all incubations are made
automatically in a compact Incubator-Shaker-Reader-Printer (Russell, 1997).
16
3.6 ADVANCED INSTRUMENTS INC. FLUOROPHOS®
®
BETASCREEN TEST (EU).
The BetaScreen® is an ELISA-based test (using fluorescence and alkaline
phosphatase as a marker). The Fluorophos® BetaScreen® Test (EU) detects six
beta-lactams: penicillin, ampicillin, amoxicillin, oxacillin, cloxacillin, and dicloxacillin.
BetaScreen® test results are available in ten minutes. The test is instrument-based
with push-button operation, microprocessor-controlled prompts and diagnostic
menus, resulting in semi-quantitative results with no operator interpretation
necessary. The Fluorophos Test System offers a digital display plus a hard copy for
a permanent record. The computer interface allows connection to laboratory
computers for analysis and reporting of data.
17
4. CONCLUSIONS
Antibiotic residue test methods based on the IDF reference method other than Delvo
SP® are appearing on the Irish market. For example, the Copan Test Kit and the
ELIPSE test kit which is manufactured by Z.E.U.-INMUNOTEC, Spain (www.zeu-
inmunotec.com) have both recently undergone validation exercises in Irish
laboratories. The ELIPSE test method is also a microbial inhibition assay for the
detection of antibiotics and sulphonamides in milk based on the IDF standard method
for determination of antibiotic residues and sulphonamides in milk.
The Parallux test kit is one of the most rapid test to carry out (< 8 minutes) and is
capable of identifying the specific antibiotic(s) detected. However, the range of
antibiotics identified is dependent on the specific assay cartridge used. Also this
method is relatively technical as it is an instrument-based test and thus, is not
suitable for use at milk intake where no laboratory facilities are available.
The Charm MRL kit has minimum sensitivities close to the EU MRL, with the
exception of Nafcillin and Cefquinom. However, the Charm MRL kit requires
separate strips for testing each of the antibiotic families as do many other rapid test
kits for the detection of residues in milk. It would be advisable to use the Charm
ROSA Reader for determination of the results as visual interpretation of test results
which involve colour change are often subjective in nature. The cost of the Charm
MRL kits escalates for sulphonamides, tetracyclines and quinolones.
The SNAP beta-lactam test, Delvo-X-Press and Beta s.t.a.r. tests only measure beta-
lactam residues in the milk. However, a separate SNAP test for tetracycline is also
available. The detection was less than or equal to the EU MRL for 8, 8, 6 and 8
compounds for the Charm MRL for beta-lactams, SNAP test for beta-lactams, Delvo-
X-Press and Beta s.t.a.r. respectively. Regarding the use of the rapid test kits as a
rapid screen for beta-lactams in raw milk, the Charm MRL for beta-lactam, SNAP test
beta-lactam assay and Beta s.t.a.r. appear to be suitable. It has been reported that
the Delvo-X-Press test is not recommended, mainly due to its insufficient test
sensitivity (Kroll, 1999). However, caution must be taken in the interpretation of
screening results on raw milk from rapid test kits which are designed to detect beta-
lactams alone, as the legal requirements for the detection of residues in milk require
the detection of a range of antibiotic residues and sulphonamides in milk, in
accordance with the EU MRL’s set out in Council Regulation No. 2377/90 and
amendments (as shown in Table 6).
It should be noted that the rapid test kits outlined are only useful for screening
purposes or positive release of tankers as inevitably the milk will have to pass a
broad spectrum microbial inhibition test (e.g. Delvo SP® test) prior to sale. Also, such
factors as high or low pH values, increased somatic cell counts, increased bacterial
counts, natural inhibitors in colostrum and compositional changes in the milk can
adversely affect the minimum sensitivities of the test methods described. However,
the examination of the influence of these factors was outside the scope of this study.
18
5. REFERENCES
AOAC Performance Test 000402. Paralluxtm Beta-lactam: A Capillary Based
Fluorescent Immunoassay For The Determination Of Penicillin-G, Ampicillin,
Amoxicillin, Cloxacillin, Cephapirin, And Ceftiofur In Bovine Milk.
[http://www.aoac.org/RI/000402.htm]
Council Directive 92/46/EEC of 16 June 1992. Laying down the health rules for the
production and placing on the market of raw milk, heat-treated milk and milk
products.
Fallon, M., O’Nuallain, N. & Hogan, G.P. (1995/1996) An outline of the current
position in relation to residues in milk and the control measures operating in this
industry in Ireland. Scientific Papers, The Food Safety Advisory Board, Ireland.
Kroll, S. (1999) Suitability of rapid test methods for the detection of residues of beta-
lactam antibiotics in milk. Dissertation, University of Munchen, Germany.
Radostitis, O.M., Blood, D.C. & Gay, C.C. (1994) Principles of antimicrobial therapy.
In: Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats
and Horses. 8th edition, pp. 153-156, Bailliere Tindall Publishers.
19
Table 6. Comparison of the EU maximum residue limits (MRL) with the sensitivities of different antibiotic test kits.
β-lactams
Penicillin 4 4 5 2 3.2 4 2-4 2-4 2-4
Penethamat 4 (2)
Ampicillin 4 10 2-3 2.9 4 4-5 4-8 2-5
Amoxicillin 4 10 2 3.6 4 6 4-8 2-4
Nafcillin 30 5 60 75-85 8-20
Cloxacillin 30 10 15 7.4 30 30 30-60 5-10
Dicloxacillin 30 10 20 25 30 25-50 5-10
Oxacillin 30 5 10 30 35-40 25-50 5-10
Cefacetril 125 20 62.5
Cefalexin 100 40-60 tbc(7) 25-50
Cefalonium 10 5-10 tbc 3-4 7.5-15
Ceftiofur(8) 100(9) 100 50 50 33.7 100 50 4-8 75-150
Cephapirin 10 20 5 16.3 10 5 4-8 8-16
Cephazolin 50 (10) 40-50 25-27.5
Cefquinom 20 (100) 20 22.5-27.5 5-20
Cefaperazon 40 tbc 5-8
20
Antibiotic EU MRL FDA Delvo Parallux Charm SNAP Delvo Beta
MRL Codex USA SP* Test* MRL* Test* X-Press* star*
(µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg)
Sulphonamides
Sulphadimidine 100(10) 25 10 equal to sulfamethazine
Sulphadimethoxine 100(10) 10 50 10
Sulphamethazine 100(10) 10 25 10
Sulphathiazole 100(10) 10 50 10
Sulphadiazine 100(10) 10 50
Tetracyclines
Chlortetracycline(8) 100(11) 100 300 150 125 30
Doxycycline 0 (100) tbc
Oxytetracycline(8) 100(11) 100 300 100 100 30
Tetracycline(8) 100(11) 100 300 100 100 30
Macrolides
Erythromycin 40 50 50
Pirlimycin 150 (50-200)
Spiramycin 200 100 200
Tilmicosin 50 50 (100)
Tylosin 50 50 50
Aminoglycosides
Gentamicin 100 200 30 100-300
Neomycin 500 500 150 100-200
Spectinomycin 200 200 (2500)
DH/Streptomycin 200 200 125 300-500
Kanamycin 2500
21
Antibiotic EU MRL FDA Delvo Parallux Charm SNAP Delvo Beta
MRL Codex USA SP* Test* MRL* Test* X-Press* star*
(µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg) (µ
µg/kg)
Quinolones
Enrofloxacin 100 (1000-1500)
Danofloxacin 30 (close to enrofloxacin)
Difloxacin 0 (close to enrofloxacin)
Flumequin 0 (no data available)
Marbofloxacin 75 (close to enrofloxacin)
Various
Bacitracin 150 (1000)
Baquiloprim 30 (60)
Chloramphenicol 0 0 2500
Clavulanic acid 200 (not antibacterial)
Colistin 50 (no data available)
Dapsone 0 1
Lincomycin 150 100
Novobiocin 50 100 (1000)
Rifaximin 60 (50)
Thiamphenicol 50 (close to chloramphenicol)
Trimethoprim 50 50
Ticarcillin N/a(12) 50
Cefadroxyl N/a 50
* It is important to note that the sensitivities of the individual test kits are based on manufacturer’s declared levels. In the case
of the Delvo SP test the values are for detection levels at control time. For reading times of 2 h 45 min or 3 hours the
sensitivity will diminish. Values for Delvo SP in parenthesis are given as indication from the DSM data-base.
22
Footnotes to Table 6.
(1)
Council Regulation (EEC) No. 2377/90 and amendments..
(2)
Codex Committee on Residues of Veterinary Drugs in Foods
(3)
Code of Federal Regulations (21 CFR 556) and Centre for Veterinary Medicine.
(4)
Different individual test kits for β-lactams, tetracyclines and sulphonamides
(5)
Different individual test kits for β-lactams, tetracyclines, sulphonamides and Quinolones, however, MRL’s unavailable for some groups to date.
(6)
Delvo-X-Press rapid test kit also detects a number of other cephalosporins not mentioned above.
(7)
tbc, denotes to be confirmed
(8)
Mother compound.
(9)
Mother compound and metabolites.
(10)
Sum of substances if this group.
(11)
Mother compound and 4-epimer.
(12)
N/a, denotes not applicable.
FURTHER INFORMATION
Further information can be obtained from:
23