Phyto Experiment Inference Result

constituent

Molish’s test: Formation of Presence of

ring carbohydrates
Sample with 2 to 3 drops of
1% α-napthol and 2 mL of
conc. H2SO4 added along the
sides.

Fehling’s test: Brick red Presence of

reducing
Sample with equal vol. of precipitate
sugars
Fehling’s solution A and B
and heated.

Benedicts test: Red Presence of

precipitate reducing
Sample treated with
sugars
Benedict’s reagent

Test for
Dragendorff’s test: Reddish brown Presence of
Carbohydrate precipitate alkaloids
Sample with Dragendorff’s
reagent

Dragendorff’s test: Reddish brown Presence of

precipitate alkaloids
Sample with Dragendorff’s
reagent

Mayer’s test: Cream colour Presence of

Test for precipitate alkaloids
Sample with Mayer’s reagent
Alkaloids

Wagner’s test: Reddish brown Presence of

precipitate alkaloids
Sample with Wagner’s
reagent

Chemical Salkowaski test: Red/Yellow Presence of

tests for color appears steroids
Steroids
Sample with CHCl3& conc.
H2SO4

Chemical Lead acetate test: Yellow colored Presence of

tests for precipitate flavonoids
To a small quantity of residue
Flavonoids
add lead acetate

Shinoda test (Magnesium Pink scarlet, Presence of

Hydrochloride reduction crimson red or flavonoids
test): Sample is treated with occasionally
Magnesium ribbon and added green to blue
concentrated Hydrochloric color
acid drop wise

Chemical Hemolysis test: Hemolytic Presence of

tests for zone appears saponins
Drug extract or dry powder is
Saponins
added to 1 drop of blood
glycosides
placed on glass slide

Ferric chloride test : Dark blue or presence of

Greenish phenolic
Ferric chloride solution added
Black colour compounds
to the extract drop wise.

Lead Acetate Test : White colour presence of

precipitate phenolic
Lead Acetate solution added
appear at compounds
to extract drop wise
Chemical test bottom of test

for Tannins tube

Gelatin Test: White colour presence of

precipitate phenolic
Freshly prepared Gelatin
appear at compounds
Solution 2% w/v added to
bottom of test
extract
tube

Foam Test: Froth revealed the

Appeared presence of
 To the plant fractions, 10-20 which saponins
ml of water was added and persisted for
shaking was done for few 60-120
minutes. seconds
Chemical test
for Saponins

Baljet test: yellow to Presence of

orange color glycosides.
To the plant extract added
appeared
sodium picrate solution drop
Chemical test wise
for Glycosides
Keller killiani test: reddish brown Presence of
layer digitoxose
To the plant fractions, equal
appeared
volume of water and 0.5 ml
which turns
of strong lead acetate
bluish green
solution were added, shaken
after standing
and filtered. Filtrate was
extracted with equal volume
of chloroform. Chloroform
extract was evaporated to
dryness and residue was
dissolved in 3 ml of glacial
acetic acid followed by

addition of few drops of ferric

chloride solution. The
resultant solution was
transferred to a test tube
containing 2 ml of conc.
sulphuric acid..

Stain test Oil stain on Ist Presence of

filter paper fixed oils
Press the small quantity of
extract between two filter
papers

Saponification test: The formation Presence of

of soap or Fixed oils and
Chemical test Add a few drops of 0.5N of partial Fats
for Saponins alcoholic potassiumhydroxide neutralization
to small quantities of various of alkali
extracts along with a drop of
Phenolphthalein separately
and heat i a water bath for 1
-2hrs.