DEMONSTRATION OF

COMMON
IMMUNOHEMATOLOGIC
REACTIONS
PART 1: DEMONSTRATION OF
AGGLUTINATION REACTIONS

OLFU-CMLS-QC
Pre-Analytical phase

■ A phlebotomist will collect blood from the patient following strict

protocol for venipuncture. The type of blood collecting tube/s to be used
depend/s on the test/s requested by the physician.

■ Upon receiving in the laboratory a properly coagulated blood specimen of

adequate volume, the medical technologist checks for proper labeling and
will duly log the information into the laboratory information system or its
alternative. Materials and devices appropriate for the test procedure
requested are then
Analytical Phase
Materials
■ Microscope Pasteur pipet Parafilm Glass slides Gum label
■ Applicator sticks

■ Gloves

■ Test tubes
■ Test tube rack Centrifuge Distilled water

■ NSS (in a wash bottle)

■ 5% RCS (any ABO-Rh positive blood)
Analytical Phase
Procedure
■ PART 1: DEMONSTRATION OF
AGGLUTINATION REACTIONS
PART 1: DEMONSTRATION OF
AGGLUTINATION REACTIONS

A. SLIDE METHOD
■ 1. Divide your glass slide
into 2 portions.

■ 2. Label one as ‘U”

(unknown) and the other as
“NC” (negative control)
PART 1: DEMONSTRATION OF
AGGLUTINATION REACTIONS

A. SLIDE METHOD
■ 3. Place a drop of reagent
in each portion
(U: anti-D, NC: NSS )
■ 4. Place a drop of blood
sample in each portion of the
glass slide.

■ 5. Mix the reagent and the

blood sample using an
applicator stick.
PART 1: DEMONSTRATION OF
AGGLUTINATION REACTIONS

A. SLIDE METHOD
■ 6. Observe the result
macroscopically and
microscopically using the LPO.
■ 7. Interpret the result within 2
minutes as positive or negative.
There is no need to grade the
reaction.
PART 1:
DEMONSTRATION
OF
AGGLUTINATION
REACTIONS

A. SLIDE METHOD
 DEMONSTRATION OF
COMMON
IMMUNOHEMATOLOGIC
REACTIONS
PART 2: DEMONSTRATION OF HEMOLYSIS
OR HEMOLYTIC REACTION

OLFU-CMLS-QC
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

1. Prepare 2 test tubes and label them as P

(expected positive) and N (expected
negative).
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

■ 2. Place 2 drops of 5% RCS of

an Rh POSITIVE blood in each
tube.
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

■ 3. Place 2 drops of distilled water

in tube labeled as P.

■ 4. Place 2 drops of NSS in tube

labeled as N.
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

■ 5. Mix and cover each tube with

parafilm.
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

■ 6. Centrifuge for 15 seconds

at 3400 rpm. Do not dislodge
the cell button.
PART 2: DEMONSTRATION OF
HEMOLYSIS OR HEMOLYTIC
REACTION

■ 7. Interpret the result.

■ 8. Compare the two
tubes and take note of
the difference.
END