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    Leinard Clavero
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    Atrial flagellates include Giardia lamblia and Chilomastix mesnili. G. lamblia has a cystic stage with 2-4 nuclei inside a double-walled cyst, and a trophozoite stage that is teardrop-shaped and binucleated with flagella and a sucking disc. C. mesnili has a lemon-shaped cyst and a pear-shaped trophozoite with 3 anterior flagella. Trichomonads such as Trichomonas vaginalis are pyriform with 4 anterior flagella and undulating membrane used for corkscrew motility. Dientamoeba fragilis is an irregular amoebaflagellate

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    Transes for atrial parasitology that will make your life easier.

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    ATRIAL-FLAGELLATES-LAB

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    Atrial flagellates include Giardia lamblia and Chilomastix mesnili. G. lamblia has a cystic stage with 2-4 nuclei inside a double-walled cyst, and a trophozoite stage that is teardrop-shaped and binucleated with flagella and a sucking disc. C. mesnili has a lemon-shaped cyst and a pear-shaped trophozoite with 3 anterior flagella. Trichomonads such as Trichomonas vaginalis are pyriform with 4 anterior flagella and undulating membrane used for corkscrew motility. Dientamoeba fragilis is an irregular amoebaflagellate

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    43 views3 pages

    Atrial Flagellates Lab

    Uploaded by

    Leinard Clavero
    Atrial flagellates include Giardia lamblia and Chilomastix mesnili. G. lamblia has a cystic stage with 2-4 nuclei inside a double-walled cyst, and a trophozoite stage that is teardrop-shaped and binucleated with flagella and a sucking disc. C. mesnili has a lemon-shaped cyst and a pear-shaped trophozoite with 3 anterior flagella. Trichomonads such as Trichomonas vaginalis are pyriform with 4 anterior flagella and undulating membrane used for corkscrew motility. Dientamoeba fragilis is an irregular amoebaflagellate

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    of 3

    ATRIAL FLAGELLATES

    Phylum: Protozoa • Variant-specific surface proteins


    Subphylum: Mastigophora • Old man with eyeglasses
    Class: Zoomastigophora • Falling/floating leaf and erratic
    Flagella tumbling motion
    Ø Slender, whip-like structure
    GIARDIA LAMBLIA Median body
    Cystic Stage
    • Ovoidal Flagella
    • Nuclei: 2-4
    • Double wall
    • Cytoplasm is retracted
    • Median bodies
    o Parabasal bodies
    • Interior flagellar structures Sucking disc
    • Cyst wall protein 1 Axoneme Axostyle
    • Football-shaped
    o Distinct morphological
    characteristic CHILOMASTIX MESNILI
    Cystic Stage
    • Lemon-shaped
    Nuclei • Knob-like or nipple-shaped
    protuberance
    • 1-2 nuclei
    • Cytosome
    Axoneme
    Median bodies Nipple-shaped

    Trophozoite Stage
    • Teardrop-shaped Cytosome
    • Bilaterally symmetrical
    • Binucleated
    • Parabasal bodies
    • Prominent axostyle Trophozoite Stage
    o Rod-like support structure • Pear-shaped
    • Four pairs of flagella • Asymmetrical
    • Longitudinal binary fission • Mononucleated
    • Ventral sucking disc • Large spherical nucleus
    o Pathological determinant o Located on the anterior
    o Once this attaches to the portion of organism
    villi, it will cause flattening • Three (3) anterior free flagella
    • Cytosomal fibril or Shepherd’s • Mononucleated
    crook • Small cytosome
    • Longitudinal spiral groove • Four (4) free qual flagella & fifth
    • Boring/spiral forward movement on the margin of UM
    • Cork-screw motility • UM: 2/3 of the costa

    Longitudinal
    Spinal Groove

    TRICHOMONAS VAGINALIS
    Trophozoite Stage
    • Pyriform TRICHOMONAS HOMINIS
    o Pear-shaped Trophozoite Stage
    • Mononucleated • Pyriform
    • Four (4) free anterior flagella • Mononucleated with small central
    • 5th flagella is along the margin of karyosome
    the undulating membrane • Five (5) anterior flagella & a
    • Prominent axostyle posterior flagellum
    • UM: half of the costa o Trailing flagellum at the
    • Siderophil granules end of UM
    • Jerky tumbling motility • Conical cytostome cleft
    • UM: as long as the costa
    • Axostyle extends from anterior to
    Axostyle posterior

    TRICHOMONAS TENAX
    Trophozoite Stage
    • Pyriform
    Amoebaflagellate
    DIENTAMOEBA FRAGILIS
    • Irregular
    • 1-2 rosette shaped nuclei
    • 4-6 chromatin granules
    • Vacuoles with ingested debris

    Chromatin
    Granules

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