What needs to happen before blood sample can be

used for PCR?

How does the DNA polymerase extend the primers
DNA has to be isolated from the cells into a new DNA strand?

Adding nucleotides to the 3' end of the primers

Great job! What is the function of primers in a PCR

reaction?
Which of these sequences is the complementary
Bind specific sites on the DNA sequence to the template sequence 5'-
GTGGTCTGATCAACGGTAA

3' - CACCAGACTAGTTGCCATT - 5'

For which enzyme are nucleotides the substrate?

DNA polmerase
When using one primer pair in different individuals
which phrase describe the PCR product?
What is the template of the PCR?
Different length may be found in each individual
DNA

What does a DNA polymerase do?

What happens to the probability of a 100% match
Synthesizes DNA between two different individuals when using 13 sets
of primers for the DNA profile instead of one?

It decreases
Why is it important to change the pipette tip?

To avoid cross contamination

How many copies of DNA are required to see bands
on the electrophoresis gel?
At this step in the PCR process, what happens to the Millions of copies
DNA?

It will be separated into two strands

Which word describes the charge of the DNA?

Negatively charged
How is the DNA separated into single strands?

The high temperature (95c)

DNA is negatively charged. To which location in the
electrophoresis gel does it migrate?
What is the step in the PCR that is now shown called? Positive pole
Annealing

So who is the murderer?

The area where the primers bind marks which part of The rival scientist
the PCR product?

Beginning 5' end

What are the building blocks of new copies of DNA?

Nucleotides
The PCR products get a certain length due to which
fact?

The placement of the primers

What is the function of primers in a PCR?

They bind specific sited on the template DNA to Why is it possible to distinguish individuals by running
initiate and direct DNA synthesis these PCR products on a gel?

The PCR products are different lengths

What would happen if no polymerase was added to

the PCR?

New DNA would not be generated

Which reagent acts as a template for the DNA

polymerase so that it knows which new DNA to make?

DNA from a blood sample

DNA polymerase binds to the template DNA. In which

direction is the new DNA subsequently synthesized?

5' to 3'

Why is the Taq-polymerase special compared to most

other polymerases?

It can resist high temperatures

What can contamination of reagents lead to?

Unreliable results

How many sets of primers are needed for DNA

profiling?

13

What is the purpose of PCR?

To copy and then make many copies if a specific

region of DNA

Why is a PCR cycle repeated 30 times?

To get enough DNA

What can DNA ladder help determine?

The length of a fragment