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BACT211

CLINICAL BACTERIOLOGY
BATCH
2021

COLLEGE OF MEDICAL LABORATORY SCIENCE-OUR LADY OF FATIMA UNIVERSITY (LAGUNA CAMPUS)

Bacterial Cytology and Morphology

Microbiology Cell wall


•study of microorganisms •Gram positive
•large and diverse group of microscopic organisms that exist as • thick peptidoglycan murein layer
single cells or cell clusters • consists of glycan chains of alternating N-acetyl-d- glucosamine (NAG)
•bacteria, parasites, fungi, viruses etc and N-acetyl-d-muramic acid (NAM)
•Bacteria • teichoic acid Peptidoglycan
--unicellular organisms • lipoteichoic acid
--lack a nuclear membrane and true nucleus
--prokaryotes •Gram negative
• inner peptidoglycan
•Classification based on cellular type: - thinner than gram (+)
1. Prokaryotes halophiles •outer peptidoglycan periplasmic space
•Archaea (Archaeobacteria) thermophiles - additional & unique to gram (-)
-extremophiles
-cell walls lack peptidoglycan
anaerobic •proteins, phospholipids, and lipopolysaccharide
methanogen
•Bacteria (Eubacteria)
2. Eukaryotes
s •Acid fast
•Fungi, algae, protozoa, animals, and plants • Mycobacterium & Nocardia Gram (+)
• waxy layer of glycolipids and fatty acids mycolic acid
Cytology

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BACT211
CLINICAL BACTERIOLOGY
BATCH
2021

COLLEGE OF MEDICAL LABORATORY SCIENCE-OUR LADY OF FATIMA UNIVERSITY (LAGUNA CAMPUS)

•Cellular organelles (eukaryotic cells) •Acid fast stain


•Endoplasmic reticulum •Ziehl-Neelsen method or Kinyoun method
- process and transport proteins •Heat or methyl alcohol
•Golgi body
- process substances for transport out of cell
•Mitochondria
- generate energy
•Lysosomes
- provide environment for controlled enzymatic degradation of
intracellular substances
•Nucleus
- provide membrane enclosure for chromosomes

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