Supplementary Table S2. Stock culture and feeding media.

Name Component Supplier & Catalog No. Final concentration Tissue type
DMEM stock Advanced DMEM/F12 Invitrogen 12634-010 1X Pancreas
culture Fetal Bovine Serum Sigma F8067-500ml 10%
medium
Penicillin/Streptomycin Invitrogen 15140-122 100 U·ml-1 / 100 mg·ml-1
ADF stock Advanced DMEM/F12 Invitrogen 12634-010 1X Colorectal, anal,
culture appendix, biliary,
Glutamax, 100x Invitrogen 35050-061 1X
medium breast, endometrial,
HEPES, 1M Fisher SH30237.01 10 mM hepatocellular, lung,
lymphoma, NET,
Penicillin/Streptomycin Invitrogen 15140-122 100 U·ml-1 / 100 mg·ml-1 ovarian, prostate
ADF feeding ADF stock (recipe above) 1X Anal, appendix, biliary,
medium breast, endometrial,
EGF, recombinant mouse Invitrogen 53003-018 50 ng/ml
hepatocellular, lung,
lymphoma, NET,
ovarian, prostate
Wnt feeding Wnt3a conditioned medium (collected in-house) 50% Colorectal
medium
ADF stock (recipe above) 50%
EGF, recombinant mouse Invitrogen 53003-018 50 ng/ml
RRD feeding DMEM stock (recipe above) 1X Pancreas
medium
R-spondin conditioned medium (collected in-house) 17%
Y27632 Sigma Y0503-5mg 20 uM
Fetal Bovine Serum Sigma F8067-500ml 7%
EGF, recombinant mouse Invitrogen 53003-018 50 ng/ml