Professional Documents
Culture Documents
The Pectinase Professionals Project: Pectinase Lab Report Descriptive Title: Background Information
The Pectinase Professionals Project: Pectinase Lab Report Descriptive Title: Background Information
The Pectinase Professionals Project: Pectinase Lab Report Descriptive Title: Background Information
Descriptive
Title:
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Background
information
First
section:
What
enzymes
are,
how
they
work,
and
what
factors
can
affect
enzymatic
activity.
Second
section:
What
is
pectinase?
Where
is
pectinase
used?
Why?
**
Important
–
document
all
of
your
sources.
Use
in-‐text
citations
and
a
bibliography**:
You
should
have
a
minimum
of
3
LEGITIMATE
sources.
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Purpose:
The
purpose
of
this
experiment
is:
–
what
are
you
trying
to
find
out?
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Independent
variable
–
what
are
you
going
to
manipulate?
pH,
temperature,
concentration.
Be
specific
In
this
experiment,
my
independent
variable
is
Dependent
variable:
-‐
What
you
test
or
measure
during
the
experiment,
the
outcome.
Be
specific.
In
this
experiment,
my
dependent
variable
is
Measured
in
(unit):
Investigable
question
format:
How
does
the
INDEPENDENT
VARIABLE
affect
the
DEPENDENT
VARIABLE?
Question:
The
question
that
we
will
investigate
during
this
lab
is:
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Hypothesis:
Your
hypothesis
is
a
statement
that
tells
the
reader
what
you
believe
the
answer
to
our
question
will
be
along
with
the
reasoning
that
supports
your
statement.
If
(change
of
indepdent
variable)_______then
(affect
on
dependent
variable)_____
BECAUSE
-‐
give
at
least
one
reason
that
is
based
on
scientific
knowledge
–
this
is
a
great
place
to
tie
in
information
from
your
background
research.
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2
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Materials:
List
items
you
need
to
complete
the
experiment.
Include
quantities!
Ex.
One
25
mL
graduated
cylinder.
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Procedure:
The
control
group
procedure
is
explained
below.
Your
job
is
to
create
a
comparable
experimental
group
procedure.
Be
sure
to
hold
your
controlled
variables
constant.
Remember
that
controlled
variables
are
any
variables
that
could
potentially
mess
up
your
results
if
they
are
not
kept
the
same
from
one
experimental
trial
to
another.
Controlled
variables
could
include
things
like
the
amount
of
applesauce
used
in
each
trial,
the
size
of
measuring
tools
(beakers),
the
accuracy
of
the
measurements,
etc.
Control:
1.Applesauce
only
–
no
enzyme:
The
class
control
will
test
25
mL
of
applesauce
at
room
temperature
(70
degrees),
with
3
ml
of
distilled
water
(added
to
equal
the
liquid
added
for
the
pectinase
solution)
and
at
the
unadjusted
pH
of
applesauce
as
it
comes
out
of
the
bottle
of
approximately
4.
The
amount
of
apple
juice
extracted
from
the
applesauce
will
be
measured
as
it
filters
through
a
coffee
filter
into
a
funnel
that
pours
into
a
250
mL
beaker.
The
juice
will
be
poured
into
a
25
mL
graduated
cylinder
and
allowed
to
drip
for
10
minutes.
After
10
minutes,
the
amount
of
juice
will
be
measured,
and
recorded
on
a
table.
This
will
be
measured
over
3
trials.
An
average
for
the
control
without
enzyme
added
will
be
computed.
2.
Applesauce
WITH
enzyme:
The
class
control
will
test
25
mL
of
applesauce
with
3
ml
concentration
of
2%
pectinase
solution
(in
distilled
water)
at
room
temperature
(70
degrees),
and
at
the
unadjusted
pH
of
applesauce
as
it
comes
out
of
the
bottle
of
approximately
4.
The
amount
of
apple
juice
extracted
from
the
applesauce
will
be
measured
as
it
filters
through
a
coffee
filter
into
a
funnel
that
pours
into
a
250
mL
beaker.
The
juice
will
be
allowed
to
collect
for
10
minutes.
The
juice
will
be
poured
into
a
25
mL
graduated
cylinder,
measured
and
recorded
on
a
table.
This
will
be
measured
over
3
trials.
An
average
for
the
control
with
enzyme
added
will
be
computed.
Experimental:
Design
a
procedure
to
test
your
independent
variable
as
described
in
your
research
question.
Be
sure
to
include
your
control
procedure,
and
your
IV
enzyme
change
procedure.
A
detailed
list
of
steps
describing
to
someone
how
to
complete
the
experiment.
S.I.R
(Specific,
In
order,
and
Replicable)
–
if
someone
had
never
seen
your
experiment,
they
should
still
be
able
to
repeat
it.
3
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Data:
Make
a
table
of
the
observations
and
data
you
collected
during
the
experiment.
Descriptive
title
of
table:
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Trial
time
elapsed
Control
group
Experimental
group
number
amount
of
juice
in
mL
amount
of
juice
in
mL
no
enzyme
with
enzyme
no
enzyme
with
enzyme
1
2
3
Average
4
Graph:
Make
a
Bar
graph
that
visually
represents
the
amount
of
juice
collected
by
each
trial.
Label
mL
Don’t
forget
to
LABEL
your
graph!
Add
all
control
and
experimental
data
and
their
averages.
The
x-‐axis
is
labeled
for
you.
You
will
need
to
label
the
y-‐axis.
Be
sure
to
add
a
legend
that
interprets
the
bar
graph
colors
or
patterns
you
choose
to
use
to
represent
the
control
and
experimental
groups.
Title
for
your
graph:
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Trial
1
Trial
2
Trial
3
Averages
Results:
This
is
not
the
place
to
state
whether
or
not
your
hypothesis
was
supported
or
not
supported
–
only
a
place
to
state
verbally
what
your
data
tables
and
graphs
show
visually.
My
results
indicate
that
(what
did
your
data
show
.
.
.
this
is
a
literal
restating,
not
an
interpretation
place
for
what
you
think
happened)
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5
Analysis
and
Conclusion:
The
critical
analysis
section;
the
place
to
interpret
and
evaluate
your
data
and
to
speculate
on
other
possibilities.
Also,
remember
that
you
can
ONLY
state
that
your
evidence
supported
your
hypothesis,
or
your
evidence
did
not
support
your
hypothesis.
Your
hypothesis
is
never
“right”
or
“wrong”
or
“correct”
or
“incorrect.”
In
this
laboratory
investigation,
we
explored
the
following
research
question:
(list
your
question):
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The
_________________________________________________________________________(your
independent
variable)
_____________
(did
or
did
not)
affect
the
______________________________________________________________________________
(your
dependent
variable
=
what
you
measured).
Thus
my
original
hypothesis
of
(re-‐state
your
hypothesis
here):
______________________
_________________________________________________________________________________________________________
was
supported/
was
not
supported
by
the
data
collected
as
shown
by
(give
2-‐3
specific
examples
of
the
data
that
either
supported
or
did
not
support
your
hypothesis
–
use
individual
data
points
as
well
as
the
averages)
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What
evidence
leads
you
to
this
conclusion
(cite
specific
evidence
from
your
data!)
How
did
the
independent
variable
_________
affect
the
dependent
variable
_______?
How
do
you
know?
(what
was
your
evidence?)
What
patterns,
cause/effect
can
you
infer
from
your
data?
How
do
you
know
this
was
the
case?
Refer
to
your
data
for
all
of
the
statements.
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At
what
point
did
you
observe
the
greatest
yield
of
apple
juice?
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Why
do
you
think
this
is
the
case?
(background
information
can
be
a
great
tool
for
explaining
at
this
point)
What
other
experiences
can
you
draw
on
that
would
support
your
reasoning?
Be
specific
in
your
examples!
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6
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Discussion:
What
factors
could
potentially
have
confounded
your
results?
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How
did
you
control
for
these
variables?
Was
the
control
method
effective?
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Were
there
any
other
weaknesses
in
your
testing
that
could
potentially
have
altered
your
results?
(yes
–
you
will
have
some
–
think
about
them
and
identify
them)
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Future
considerations:
Ways
to
improve
this
experiment
include…
What
variables
could
have
had
an
unintended
impact
on
your
experimental
results?
What
could
you
do
to
control
those
variables
if
you
were
to
do
this
experiment
again?
What
flaws
did
you
notice
in
your
experimental
design?
How
could
those
flaws
be
remedied
if
you
were
to
do
the
experiment
again?
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7
Why
does
your
experiment
matter
to
other
researchers?
Can
you
generalize
your
results
to
other
situations
where
the
information
you
gained
from
your
experiment
could
be
helpful?
(HINT:
go
back
to
the
purpose
of
your
lab
at
the
top)
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What
suggestions
do
you
have
for
the
direction
future
research
could
take
from
your
research
–
what
questions
are
still
unanswered
that
are
specific
to
the
research
that
you
just
completed?
What
other
questions
does
your
research
lead
you
to?
What
other
question
would
you
ask
and
investigate
as
a
continuation
of
the
research
you
just
completed?
Why?
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Bibliography
All
citations
and
background
information
sources
should
be
listed
here
in
alphabetical
order
by
Author’s
last
name.
Use
APA
formatting.
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8
10
Teacher
Information
This
lab
is
designed
for
students
who
are
learning
about
enzymes.
The
skills
that
are
the
focus
of
this
lab
activity
include:
• Generalizing
information
about
factors
that
affect
enzymatic
activity
(pH,
temperature
and
concentration
of
enzyme/substrate)
• Formatting
a
testable
question
• Stating
a
hypothesis
that
is
supported
by
scientific
reasoning
• Developing
a
procedure
to
test
the
hypothesis
that
is
specific,
written
in
order,
and
is
replicable
(S.I.R.
is
the
acronym
I
use
for
procedures)
• Becoming
aware
of
potential
sources
of
error
• Measuring
data
• Analyzing
data
to
draw
a
conclusion
.
.
.
and
making
sure
that
the
conclusion
can
be
tied
back
to
the
original
lab
question
and
hypothesis
• Discussing
the
nature
of
scientific
research
and
conclusions
based
on
evidence
• Creating
a
formal
lab
write
up
based
on
information
collected
in
the
lab
packet
I
use
this
lab
in
a
MYP
International
Baccalaureate
biology
class,
though
it
is
really
appropriate
for
any
advanced
middle
school
or
high
school
class.
We
are
on
a
block
schedule
with
80-‐minute
class
periods.
The
timeline
I
use
for
this
lab
is:
Day
1:
• Brief
introduction
to
the
upcoming
lab
• Homework
assignment:
complete
background
research.
Day
2:
• As
soon
as
students
walk
in
the
door
I
stamp
their
lab
packets
for
completion
of
background
information.
Students
who
are
missing
a
stamp
when
the
packet
gets
turned
in
lose
points
on
the
background
research
portion
of
the
lab
grade.
• Lab
groups
assigned
(I
try
to
keep
the
labs
as
either
partners
or
3
people
in
a
lab
group
–
no
more).
Lab
groups
work
together
to
compare
background
information
and
collaborate
to
create
a
cohesive
background
information
section
(with
all
citations).
• By
the
end
of
class
students
should
have:
o Cohesive
and
relevant
background
information
with
citations
o Clearly
identified
independent
and
dependent
variables
o Question
o Hypothesis
with
supporting
information
from
background
research
o Experimental
procedure
that
is
detailed
and
meets
the
S.I.R.
requirements
o Bibliography
• I
have
groups
raise
their
hands
each
time
they
finish
a
section
so
that
I
can
give
feedback
during
the
process
of
planning
their
experiments.
If
they
have
met
the
requirements
for
the
section
that
I
review,
I
stamp
their
lab
packet
(i.e.
When
student
questions
are
phrased
in
a
way
that
is
measurable,
I
stamp
the
“question”
part
of
the
lab
packet)
• Students
should
be
ready
to
conduct
their
lab
experiment
by
the
end
of
class.
Day
3:
• As
soon
as
students
walk
in
the
door
I
review
their
lab
packets
for
stamps.
Students
missing
stamps
may
not
proceed
to
the
experiment
until
all
sections
are
stamped.
Students
who
have
11
wasted
time
and
do
not
get
the
lab
packet
completed
in
a
timely
manner
(for
me
this
means
within
10
minutes
of
the
start
of
class)
must
write
a
report
about
enzymes
rather
than
completing
the
lab.
• Students
complete
all
of
the
data
collection
by
the
end
of
class.
Day
4:
• Students
collaborate
to
analyze
their
data
and
generate
a
conclusion.
• In
my
class
I
allow
students
to
begin
working
on
their
formal
lab
write
up
in
class
using
laptop
computers.
• By
the
end
of
class
students
should
have:
o A
completed
lab
packet
o If
doing
a
formal
lab
write
up,
students
should
have
started
the
initial
portions
of
their
lab
write
up.
• Homework:
Finish
the
formal
lab
write
up.
Helpful
hints
for
grading:
I
grade
this
lab
in
two
parts:
the
lab
packet,
and
the
formal
lab
write
up.
I
also
include
a
group
participation
grade
and
look
carefully
at
the
ratings
that
the
team
members
give
to
each
other
to
try
and
identify
areas
of
weakness,
students
who
might
be
struggling
with
group
work,
etc.
I
also
look
at
the
group
evaluations
and
compare
them
against
my
own
observations
of
student
group
work.
When
students
are
obviously
dishonest
in
their
evaluations,
I
talk
with
them
privately
to
figure
out
if
there
is
more
to
the
story
–
and
depending
on
the
situation
I
may
give
them
a
chance
to
revise
their
group
evaluation
scores.
If
a
student
is
blatantly
dishonest
or
is
covering
for
a
friend
who
did
not
contribute,
the
student
who
was
dishonest
loses
group
work
points.
Because
I
teach
in
the
International
Baccalaureate
Programme,
I
use
IB
rubrics
to
grade
labs.
The
last
pages
of
this
lab
packet
are
the
MYP
rubrics
that
I
use
to
grade
the
lab
write
up.
Students
review
the
IB
rubrics
prior
to
starting
the
lab.
In
addition,
I
have
students
highlight
their
“evidence”
within
their
paper
of
where
they
accomplished
the
task
descriptor
as
set
forth
in
the
rubric
and
I
have
them
write
the
location
of
the
“evidence”
on
their
rubric
page.
This
helps
me
to
see
if
students
understand
what
the
task
descriptors
or
mark
bands
require.
Any
grading
rubrics
for
labs
can
be
applied
to
this
lab
–
not
just
IB
rubr.
Happy
Pectinasing!
If
you
would
like
a
more
advanced
version
of
this
lab,
please
check
back
to
my
store.
The
advanced
version
requires
students
to
include
a
null
hypothesis,
standard
error,
multiple
group
comparisons,
and
a
more
complex
analysis
and
discussion.
Rebecca Peterson, M. Ed.
Rebecca
Peterson,
M.
Ed.
Becca’s Biome © 2015
Pabcdefghijklmnoqrstp
12
Pectinase
Lab
Criterion
B:
Inquiring
and
Designing
i.
Explain
a
problem
or
question
to
be
tested
by
a
scientific
investigation.
ii.
Formulate
a
testable
hypothesis
and
explain
it
using
scientific
reasoning.
iii.Explain
how
to
manipulate
the
variables,
and
explain
how
data
will
be
collected.
iv.
Design
scientific
investigations.
Achieve Task
Specific
Level
Descriptors
Evidence in your work of achievement level
ment
Level
(The
descriptors
that
are
made
more
specific
for
this
particular
task)
• Be
specific.
• Highlight
the
criteria
key
words
• Highlight
where
you
demonstrated
the
key
items
in
your
• Write
where
your
“proof”
can
be
found
in
this
column
ne
0
Student
does
not
complete
the
assignment.
1-‐2
i.
State
a
question
about
factors
that
affect
pectinase
activity
i.
o minimal
background
information
to
provide
context
for
the
question.
ii.
ii. State a testable hypothesis that is brief.
iii. State the dependent and independent variables with minimal success. iii.
iv.
Design
a
method,
with
limited
success.
o Step-‐by-‐step
instructions
are
outlined
in
general
terms.
iv.
o Elements
of
S.I.R.
(Specific,
In
order
and
Replicable)
are
partially
present.
3-‐4
i.
Outline
a
question
about
factors
that
affect
pectinase
activity.
i.
o Question
contains
the
independent
and
dependent
variable.
o
Question
is
appropriate
for
the
background
information
and
context.
ii.
ii.
Formulate
a
testable
hypothesis
using
basic
scientific
reasoning.
iii.
State
the
dependent
and
independent
variables
with
success.
o Outline
how
to
manipulate
the
independent
variable.
iii.
o Outline
how
relevant
data
will
be
collected.
iv.
Design
a
safe
method
in
which
he
or
she
selects
materials
and
equipment.
iv.
o Write
a
procedure
that
is
Specific,
In
Order,
and
Replicable
with
moderate
success.
o Someone
following
your
procedure
could
partially
replicate
your
experiment
using
only
your
procedure
for
direction.
5-‐6
i.
Describe
a
problem
or
question
to
be
tested
by
a
scientific
investigation
i.
about
factors
that
affect
pectinase
activity.
o Description
contains
the
independent
and
dependent
variable,
as
well
as
detail
about
the
problem
to
be
investigated.
ii.
ii. Formulate and explain a testable hypothesis using scientific reasoning.
o Reference
is
made
to
your
background
information
as
support
for
your
reasoning.
iii.
iii. Describe how to manipulate the variables, and describe how sufficient,
relevant data will be collected.
iv.
Design
a
complete
and
safe
method
in
which
he
or
she
selects
iv.
appropriate
materials
and
equipment.
o Write
a
procedure
that
is
Specific,
In
Order,
and
Replicable
with
success.
o Someone
following
your
procedure
could
replicate
your
experiment
using
only
your
procedure
for
direction
with
only
a
few
questions.
13
7-‐8
i.
Explain
a
problem
or
question
to
be
tested
by
a
scientific
investigation
i.
about
factors
that
affect
pectinase
activity.
o Explanation
contains
the
independent
and
dependent
variable,
as
well
as
detail
about
the
relevance
of
the
problem
to
be
investigated
ii.
o Context
for
the
experiment
is
established
(what
is
the
question
and
why
does
it
matter?).
ii. Formulate and explain a testable hypothesis using correct scientific
reasoning. iii.
o Reasoning
is
evident
in
statements
and
explanatory
sentences
in
your
lab
write-‐up.
o Answer
the
question,
“why
do
I
think
this
hypothesis
may
be
supported
by
my
data?”
iv.
o Background
information
is
used
to
support
scientific
reasoning
for
the
hypothesis.
iii. Explain how to manipulate the variables, and explain how sufficient,
relevant data will be collected.
iv.
Design
a
logical,
complete
and
safe
method
in
which
he
or
she
selects
appropriate
materials
and
equipment.
o Write
a
procedure
that
is
Specific,
In
Order,
and
Replicable
with
clear
success.
o Someone
following
your
procedure
could
replicate
your
experiment
using
only
your
procedure
for
direction
without
any
questions.
14
Pectinase Lab
Criterion c: Processing and Evaluating
i. Present collected and transformed data.
ii. Interpret data and explain results using scientific reasoning.
iii. Evaluate the validity of a hypothesis based on the outcome of the scientific investigation.
iv. Evaluate the validity of the method.
v. Explain improvements or extensions to the method.
Achiev Task Specific
ement
Evidence in your work of achievement level
Level
(The descriptors that are made more specific for this particular task) • Be specific.
• Highlight the criteria key words
• Highlight where you demonstrated the key items in
your work.
• Write where your “proof” can be found in this column
next to the level you achieved.
0 Student does not complete the assignment.
15
5-6 i. Correctly collect, organize and present data in numerical and/or visual i.
forms.
o Tables
and
graphs
have
labels
identifying
what
they
represent.
o Only
very
minor
errors
are
present.
ii. Accurately interpret data and explain results using scientific reasoning. ii.
o Have
you
discussed
how
you
interpreted
your
data?
o Have
you
identified
any
patterns
or
trends?
o Have
you
included
data
from
your
experiment
in
your
results
section?
iii. Discuss the validity of a hypothesis based on the outcome of a scientific iii.
investigation.
o Is
your
hypothesis
supported
or
not?
o Is
this
discussed
with
3-‐4
sentences
to
support
your
statement?
iv. Discuss the validity of the method based on the outcome of a scientific
investigation. iv.
o Does
your
procedure
help
you
to
investigate
your
hypothesis
and
answer
your
question?
o Have
you
discussed
how
your
method
will
answer
your
question?
o Why
do
you
think
your
procedure
will
work?
v. Describe improvements or extensions to the method that would benefit the v.
scientific investigation.
o What
could
you
change
to
improve
your
experiment?
o Describe
those
changes
7-8 i. Correctly collect, organize, transform and present data in numerical and/or i.
visual forms.
ii. Accurately interpret data and explain results using correct scientific
reasoning.
o Explain
what
significance
any
errors
in
measurement
might
have
ii.
on
your
data.
iii. Evaluate the validity of a hypothesis based on the outcome of a scientific
investigation.
o Is
your
hypothesis
supported
or
not?
o
Is
this
explained
so
that
your
reasoning
is
clear?
iii.
o How
do
you
know
your
hypothesis
is
or
is
not
supported?
iv. Evaluate the validity of the method based on the outcome of a scientific
investigation.
o Does
your
procedure
help
you
to
investigate
your
hypothesis
and
answer
your
question?
iv.
o Have
you
explained
how
your
method
will
answer
your
question?
o Why
do
you
think
your
procedure
will
work?
o Have
you
compared
your
method
compared
to
other
options?
o Have
you
stated
why
or
why
you
would
not
choose
another
method?
Advantages?
Disadvantages?
v.
v. Explain improvements or extensions to the method that would benefit the
scientific investigation.
o What
could
you
choose
to
improve
your
experiment?
Why?
o What
is
your
reasoning
for
making
those
recommendations?
o What
questions
are
a
logical
extension
of
your
research?
16