Professional Documents
Culture Documents
Medios Micro Alimentos
Medios Micro Alimentos
Medios Micro Alimentos
References Availability
1. Hajna and Perry. 1943. Am. J. Public Health 33:550.
2. Tennant, Reid, Rockwell and Bynoe. 1961. Can. J. Microbiol. 1:733.
Difco™ EC Medium
3. Fishbein and Surkiewicz. 1964. Appl. Microbiol. 12:127. AOAC BAM CCAM COMPF EPA ISO SMD SMWW
4. Eaton, Rice and Baird (ed.). 2005. Standard methods for the examination of water and wastewater,
21st ed., online. American Public Health Association, Washington, D.C. Cat No. 231420 Dehydrated – 100 g
5. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 231430 Dehydrated – 500 g
4th ed. American Public Health Association, Washington, D.C.
6. Wehr and Frank (ed.). 2004. Standard methods for the examination of dairy product, online. American
231410 Dehydrated – 10 kg
Public Health Association, Washington, D.C.
7. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC Interna-
tional, Gaithersburg, Md.
8. Horwitz (ed.). 2007. Official methods of analysis of AOAC International, 18th ed., online. AOAC
International, Gaithersburg, Md.
9. Ray. 1986. J. Food. Prot. 49:651.
Cultural Response
Difco™ EC Medium with MUG
Prepare the medium per label directions. Inoculate tubes in duplicate with fresh 18-24 hour cultures. Incubate
the first set at 35 ± 2°C for 24 ± 2 hours and the second set at 44.5 ± 0.2°C for 24 ± 2 hours. Read fluorescence
under a long-wave UV light.
RECOVERY at RECOVERY at
Organism ATCC™ 35°C/Gas 44.5°C/Gas Fluorescence
Enterobacter aerogenes 13048 Good/± Inhibition to good/– –
Enterococcus faecalis 19433 Inhibition/– Inhibition to good/– –
Escherichia coli 25922 Good/+ Good/+ + Escherichia coli
ATCC™ 25922
201
EC Medium, Modified
Novobiocin Antimicrobic Supplement
Intended Use Principles of the Procedure
EC Medium, Modified is used with Novobiocin Antimicrobic Peptone supports good growth of E. coli O157:H7 and is rich
Supplement in the detection of Escherichia coli O157:H7 in in peptides and nitrogen. Lactose is an additional source of
meat and poultry products. carbon for organisms, such as E. coli, that can ferment this
sugar. Dipotassium phosphate and monopotassium phosphate
Summary and Explanation are buffers that facilitate recovery of injured cells. Sodium
EC Medium, Modified and Novobiocin Antimicrobic Supple- chloride provides a suitable ionic environment for growth of
ment are based on the formula for modified EC broth with microorganisms.
novobiocin (mEC+n) as described by Okrend and Rose.1 In Selectivity of the medium is achieved by the incorporation of Bile
modifying the EC Medium formula, Okrend and Rose reduced Salts No. 3 into the base medium and by the addition of sodium
the Bile Salts No. 3 from 1.5 g per liter to 1.12 g per liter and novobiocin to the complete medium. These agents suppress
added 20 mg per liter of sodium novobiocin. Okrend et al. the growth of nuisance organisms commonly found in foods.
reported that mEC+n was useful in the enrichment and detection The sodium novobiocin is provided in the freeze-dried state
of E. coli O157:H7 from meats and poultry products.2-4 as Novobiocin Antimicrobic Supplement. This supplement is
rehydrated before use with sterile purified water.
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Directions for Preparation from NOTE: Refrigerated broth generally becomes cloudy or forms
Dehydrated Product precipitates but clears upon warming to room temperature.
Difco™ Lauryl Tryptose Broth However, clarity is not important because only gas production
1. Suspend 35.6 g of the powder in 1 L of purified water. Mix is significant.
thoroughly.
2. Warm slightly to completely dissolve the powder.
Procedure
Refer to the official test procedures for the detection of coliforms
3. Dispense required amounts into tubes containing inverted
in the compendia of methods for microbiological examination
fermentation vials (see table).
of foods, dairy products and waters.2-5
4. Autoclave at 121°C for 15 minutes. Cool the broth as quickly
as possible.
Expected Results
5. Test samples of the finished product for performance using
After incubation of the tubes with loosened caps at 35 ± 0.5°C
stable, typical control cultures.
for 24 hours, examine for turbidity and for gas production in
BBL™ Lauryl Sulfate Broth the Durham fermentation tubes. If no gas has formed and been
1. Suspend 35.6 g of the powder in 1 L of purified water. trapped in the inverted tube, reincubate and reexamine after
2. Dispense in test tubes, containing inverted Durham tubes, 48 hours.2-5
in 10 mL amounts for testing samples of 1 mL or less. For Turbidity of the medium accompanied by formation of gas in
testing 10 mL quantities of samples, dissolve 71.2 g of the any amount in the Durham tubes within 48 hours is a positive
powder in 1 L of purified water and distribute in 10 mL presumptive test for the presence of coliforms in the sample.2-5
amounts. The concentration of the medium should be The result should be confirmed by additional standard testing.
varied according to the size of the test samples (see table).
3. Autoclave at 121°C for 15 minutes. After autoclaving, cool References
the broth as quickly as possible. 1. Mallmann and Darby. 1941. Am. J. Public Health 31:127.
2. Downes and Ito. 2001. Compendium of methods for the microbiological examination of foods. 4th
4. Test samples of the finished product for performance using ed. American Public Health Association, Washington, D.C.
3. Wehr and Frank (ed.). 2004. Standard methods for the examination of dairy products, 17th ed.
stable, typical control cultures. American Public Health Association. Washington, D.C.
4. Eaton, Rice and Baird (ed.). 2005. Standard methods for the examination of water and wastewater,
Preparation of Lauryl Tryptose (Sulfate) Broth4 21st ed., online. American Public Health Association, Washington, D.C.
5. Horwitz (ed.). 2007. Official methods of analysis of AOAC International. 18th ed., online. AOAC
amount of dehydrated International. Gaithersburg, Md.
medium in volume of medium
Inoculum mL the tube mL medium+inoculum mL required g/L
1 10 or more 11 or more 35.6 Availability
10 10 20 71.2 Difco™ Lauryl Tryptose Broth
AOAC BAM CCAM COMPF ISO SMD SMWW
10 20 30 53.4
Cat. No. 224140 Dehydrated – 100 g
20 10 30 106.8 224150 Dehydrated – 500 g
100 50 150 106.8 224120 Dehydrated – 2 kg
100 35 135 137.1 224130 Dehydrated – 10 kg
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