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    BIO 024 LAB ACTIVITY 2 PH Buffers

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    Theresa Marie Baniqued Tejada
    This document outlines an experiment on pH and buffers, including discussing pH, the Henderson-Hasselbalch equation, and using pH paper and meters. It provides a table to classify samples as acidic or basic and questions about defining pH and buffers, the importance of pH, why blood pH remains constant, and calculating H+ and OH- concentrations and the pH of a buffer solution.

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    BIO 024 LAB ACTIVITY 2 PH Buffers

    Uploaded by

    Theresa Marie Baniqued Tejada
    15 views3 pages
    This document outlines an experiment on pH and buffers, including discussing pH, the Henderson-Hasselbalch equation, and using pH paper and meters. It provides a table to classify samples as acidic or basic and questions about defining pH and buffers, the importance of pH, why blood pH remains constant, and calculating H+ and OH- concentrations and the pH of a buffer solution.

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    BIO-024-LAB-ACTIVITY-2-pH-Buffers

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    This document outlines an experiment on pH and buffers, including discussing pH, the Henderson-Hasselbalch equation, and using pH paper and meters. It provides a table to classify samples as acidic or basic and questions about defining pH and buffers, the importance of pH, why blood pH remains constant, and calculating H+ and OH- concentrations and the pH of a buffer solution.

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    © All Rights Reserved
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    Flag for inappropriate content
    Download as pdf or txt
    15 views3 pages

    BIO 024 LAB ACTIVITY 2 PH Buffers

    Uploaded by

    Theresa Marie Baniqued Tejada
    This document outlines an experiment on pH and buffers, including discussing pH, the Henderson-Hasselbalch equation, and using pH paper and meters. It provides a table to classify samples as acidic or basic and questions about defining pH and buffers, the importance of pH, why blood pH remains constant, and calculating H+ and OH- concentrations and the pH of a buffer solution.

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    of 3

    PHINMA UNIVERSITY OF PANGASINAN

    COLLEGE OF ALLIED HEALTH SCIENCES


    DEPARTMENT OF NURSING

    BIOCHEMISTRY LABORATORY

    NAME:_________________ DATE: _________________


    GROUP NO. :____________ RATING: _______________

    ACTIVITY NO. 2
    pH and BUFFERS

    I- DISCUSSION:
    pH, hydronium ion concentration, buffers
    Henderson-Hasselbalch Equation: pH = pKa + log
    Use of pH paper (litmus paper) and pH meter

    II- PROCEDURE:
    A. DETERMINATION OF pH
    1. Classify the following substances if it is acidic or basic. Completyie the table below:

    SAMPLE CLASSIFICATION

    1. milk  
    2. egg white  
    3.vinegar  
    4.coke  
    5.pepsi  
    6. coffee  
    7. tea  
    8. calamansi juice  
    9. chlorox  
    10.shampoo  
    11. soap solution  
    12. detergent solution  
    13. rubbing alcohol  
    14. hydrogen peroxide  
    15. beer  
    16. baking soda soln.  
    III- QUESTIONS:

    1. Give the meaning of pH and buffer.

    2. Give the importance of pH.

    3. Why is it that the pH of body fluids like blood remains virtually constant?

    4. Calculate the [H+] and [OH-] of a solution with pH 5.6

    5. Calculate the pH of the buffer solution formed when 0.3M acetic acid (Ka = 1.8 x 10-5) is
    added to 0.2M sodium acetate.

    IV- Reference/s used: (APA format 7th edition)

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