BACHELOR OF SCIENCE IN MEDICAL TECHNOLOGY /

MEDICAL LABORATORY SCIENCE

COURSE OUTLINE IN HEMATOLOGY 2

(CMO 13 series 2017)

Course Title: HEMATOLOGY 2

Course Credit: 3 units (2 units lecture and 1 unit laboratory)
Contact Hours: Lecture: 2 hours per week (36 hours)
Laboratory: 3 hours per week (54 hours)
Placement: 3rd Year, 2nd Semester
Pre-requisites: Hematology 1

Course Description:

The course deals with the study of the fundamentals, pathophysiology and the mechanism
of coagulation and hemostasis, with emphasis on laboratory diagnostic procedures

Course Outcomes:

At the end of this course, the student should be able to:

1. explain the concepts and principles of primary hemostasis, coagulation and fibrinolysis;
2. demonstrate manual dexterity in the performance of laboratory procedures in evaluating
defects in primary hemostasis, coagulation and fibrinolysis;
3. compare the different tests in primary hemostasis, coagulation and fibrinolysis, as well
as their significance in clinical evaluation;
4. differentiate the pathophysiology of various hematologic and bleeding disorders;
5. evaluate traditional and advanced techniques, and their value, in diagnosis of
hematologic and bleeding disorders; and
6. appreciate the evolution of tests, from traditional to advanced, in the diagnosis of
hematologic and bleeding malignancies.

Course Topic Outline:

I. Platelets (Thrombocytes)

A. Megakaryopoiesis
B. Platelet Structure
C. Platelet Function
D. Abnormal Platelet Structure

II. Tests for Platelets

A. Platelet Counting
B. Platelet Estimation
C. Platelet Function Tests
D. Other Tests for Platelets
E. Automation and Quality Control
III.Hemostasis

A. Basic Principles of Hemostasis

B. Primary Hemostasis
C. Secondary Hemostasis

IV. Coagulation

A. Coagulation Factors
B. Coagulation Pathways
C. Inhibitors of Coagulation

V. Fibrinolysis

A. Mechanism of Fibrinolysis
B. Fibrinolytic Pathway
C. Inhibitors of Fibrinolysis

VI. Laboratory Evaluation of Coagulation, Hemostasis and Fibrinolysis

A. Specimen Collection, Transport and Processing

B. PT and APTT
C. Other/ Special Coagulation, Fibrinolysis and Hemostasis Tests
1. Thrombophilia Testing
2. Fibrinolytic Assays
3. Genetic Tests
4. Miscellaneous Tests
5. Novel/Global Tests for Hemostasis
D. Automation, Instrumentation and Quality Control

VII.Bleeding Disorders

A. Platelet Disorders
1. Quantitative Platelet Disorders
2. Qualitative Platelet Disorder

B. Disorders due to Vascular Defects

C. Coagulation Disorders
1. Congenital/ Hereditary
2. Acquired

D. Disorders due to Presence of Circulating Anticoagulants

VIII. Leukemia and Other Malignancies

A. Acute Leukemia
B. Chronic Leukemia
C. Lymphoid and Plasma Cell Neoplasms
D. Myeloproliferative Neoplasms
E. Myelodysplastic Syndromes

IX.
LABORATORY EXERCISES:

1. Platelet Count
2. Platelet Estimation (Blood Smear)
3. Bleeding Time (Ivy Method)
4. Activated Clotting Time (ACT)
5. Capillary Fragility Test
6. Prothrombin Time
7. Computation of ISI and INR
8. Activated Partial Thromboplastin Time
9. APTT Mixing Studies
10. Thrombin Time
11. Fibrinogen Assay

REFERENCES:

Textbook

1. Keohane, E. et.al (2019). Rodak’s Hematology: Clinical Principles and Applications

(6th edition).

References

1. Turgeon, M. L. (2018). Clinical Hematology: Theory and Procedures (6th edition).

2. Ciesla, B. (2018). Hematology in Practice (3rd edition).
3. Greer, J., et al. (2018). Wintrobe’s Clinical Hematology (14th edition).
4. Bain, B., et al. (2017). Dacie and Lewis Practical Haematology (12th edition).
5. McPherson, R. & Pincus, M. (2017). Henry’s Clinical Diagnosis and Management by
Laboratory Methods (23rd edition).
6. Orillaza, M. et al. (2012). PCQACL’s Standardization and Harmonization of
Complete Blood Count in the Philippines (1st edition).
7. Campo, E. et al. (2017). WHO Classification of Tumours of Hematopoeitic and
Lymphoid Tissues (4th edition).

Journals

1. Blood: American Society of Hematology. http://www.ashpublications.org/blood

2. International Journal of Laboratory Hematology: International Society for Laboratory
Hematology
3. LabMedicine: American Society for Clinical Pathology
4. Journal of Thrombosis and Haemostasis: International Society on Thrombosis and
Haemostasis
5. Research and Practice in Thrombosis and Haemostasis: International Society on
Thrombosis and Haemostasis

Applications - Virtual Simulation Laboratory

1. Labster.  https://www.labster.com

Other References

1. International Council for Standardization in Hematology. https://www.icsh.org

2. International Society for Laboratory Hematology. https://www.islh.org
3. American Society of Hematology. https://www.hematology.org
4. Practical Haemostasis. https://practical-haemostasis.com/
5. International Society on Thrombosis and Haemostasis. https://www.isth.org/
Prepared by:

HEMATOLOGY CORE GROUP

DEAN CHARITO M. BERMIDO

Centro Escolar University - Manila

DEAN ZENAIDA C. CAJUCOM

Unciano Colleges - Antipolo

DEAN BERNARD U. EBUEN

Arellano University

DEAN MARK RAYMUND G. NAVA

Our Lady of Guadalupe Colleges