ARELLANO UNIVERSITY

MEDICAL LABORATORY SCIENCE PROGRAM-JUAN SUMULONG CAMPUS

2600 Legarda St., Sampaloc, Manila
www.arellano.edu.ph

BACHELOR OF SCIENCE IN MEDICAL TECHNOLOGY /

MEDICAL LABORATORY SCIENCE

COURSE OUTLINE IN HEMATOLOGY 1

(CMO 13 series 2017)

Course Title: HEMATOLOGY 1

Course Credit: 4 units (3 units lecture and 1 unit laboratory)
Contact Hours: Lecture: 3 hours per week (54 hours)
Laboratory: 3 hours per week (54 hours)
Placement: 3rd Year, 1st Semester
Pre-requisites : Histology, Biochemistry

Course Description:

This course deals with the study of blood as a tissue and the pathophysiology of the cellular
elements of blood. Emphasis is on laboratory diagnostic procedures.

Course Outcomes:

At the end of this course, the student should be able to:

1. Explain the factors and principles of hematological determinations,

2. Recognize microscopically the different normal and abnormal blood cells,
3. Demonstrate competency in the collection, handling and transportation of specimen for
hematology tests,
4. Correlate the importance of hematological tests in the diagnosis and prognosis of diseases,
5. Apply concepts of quality assurance in Hematology,
6. Perform hematological tests with precision, accuracy and reliability, and
7. Develop the necessary skills such as technical, clinical, mathematical and judgment skills in
performing Hematology tests.

Course Topic Outline:

I. Introduction to Hematology
A. Overview of Hematology
B. Safety in Hematology Laboratory
C. Basic Hematological Methods of Examination
D. Specimen Consideration in Hematology Testing

II. Hematopoiesis/ Cell Kinetics

A. General Principles of Hematopoiesis
B. Erythropoiesis
C. Leukopoiesis
D. Thrombopoiesis
E. Nomenclatures

III. The Erythrocytes (Red Blood Cells)

A. Normal Mature Red Cell Structure and Functions
B. RBC Production and Destruction
C. RBC Metabolism and Membrane Physiology
D. Abnormal RBC Morphology
 IV. Hemoglobin
A. Hemoglobin Synthesis
B. Functions
C. Normal and Abnormal Variants
D. Catabolism of Hemoglobin

V. The Leukocytes (White Blood Cells)

A. Normal WBC Morphology
B. Functions
C. Abnormal WBC Types and Morphology

VI. Complete Blood Count

A. Hemocytometry
B. Hemoglobin Determination
C. Hematocrit Determination
D. Differential Leukocyte Count
E. Reference Range and Reporting Units
F. Examination of Peripheral Blood Smear
G. Automated Cell-Counting Instrumentation

VII. Other Routine Hematology Tests

A. RBC Indices and Novel Blood Cell Parameters
B. Reticulocyte Count
C. Erythrocyte Sedimentation Rate

VIII. Special Hematology Tests

A. Bone Marrow Examination
B. Hemoglobin Electrophoresis
C. Cytochemistry/Special Staining
D. Molecular Diagnostics/ Leukemia Genetic Testing
E. Flow Cytometric Analysis
F. Other Historical Special Tests of Clinical Importance

IX. Quality Assurance and Quality Control

X. Non-Malignant Blood Disorders

A. Red Blood Cell Disorders

1. Anemia
2. Polycythemia
3. Hemoglobinopathies
4. Thalassemias
5. Porphyrias

B. White Blood Cell Disorders

1. Hereditary
2. Non-Hereditary

LABORATORY EXERCISES:

1. Blood Collection Techniques for Hematology Tests

a. Venipuncture
b. Capillary Puncture
2. Hemocytometry
3. Blood Smear Preparation
4. Complete Blood Count
a. Hemoglobin Determination
b. Hematocrit Determination
c. Red Blood Cell Count
d. White Blood Cell Count
 e. Differential WBC Count
5. RBC Indices Computation
6. Reticulocyte Count
7. Erythrocyte Sedimentation Rate
8. Prussian Blue Staining/Perl’s Iron Staining

REFERENCES:

Textbook

1. Keohane, E. et.al (2019). Rodak’s Hematology: Clinical Principles and Applications (6th
edition).

References

1. Turgeon, M. L. (2018). Clinical Hematology: Theory and Procedures (6th edition).

2. Ciesla, B. (2018). Hematology in Practice (3rd edition).
3. Greer, J., et al. (2018). Wintrobe’s Clinical Hematology (14th edition).
4. Bain, B., et al. (2017). Dacie and Lewis Practical Haematology (12th edition).
5. McPherson, R. & Pincus, M. (2017). Henry’s Clinical Diagnosis and Management by
Laboratory Methods (23rd edition).
6. Orillaza, M. et al. (2012). PCQACL’s Standardization and Harmonization of Complete Blood
Count in the Philippines (1st edition).
7. Campo, E. et al. (2017). WHO Classification of Tumours of Hematopoeitic and Lymphoid
Tissues (4th edition).

Journals

1. Blood: American Society of Hematology. http://www.ashpublications.org/blood

2. International Journal of Laboratory Hematology: International Society for Laboratory
Hematology
3. LabMedicine: American Society for Clinical Pathology

Applications - Virtual Simulation Laboratory

1. Labster.  https://www.labster.com
2. Cellavision. https://www.cellavision.com

Other References

1. International Council for Standardization in Hematology. https://www.icsh.org

2. International Society for Laboratory Hematology. https://www.islh.org
3. American Society of Hematology. https://www.hematology.org

Grading System

LECTURE: AQ (Quizzes) = 10%; AA (Activities) = 20%; AR (Recitation) = 5%; SE (Summative

Exam) = 20%; TE (Term/Major Exam) = 40%
LABORATORY: AQ (Quizzes) = 10%; AA (Activities) 25%; AT (Attendance) = 5%; SE (Practical
Exam) = 30%; TE (Term/Major Exam) = 30%
COURSE GRADE: 50% + LABORATORY 50%

Prepared by:

BERNARD U. EBUEN
Program Chair
Reviewed and Approved by:

BERNARD U. EBUEN
Program Chair