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4891148translate Ru en 817702-Srs-Difteriya
4891148translate Ru en 817702-Srs-Difteriya
The material is taken from the slant agar, placed in a drop physiological
solution on a glass slide, a thin smear is made, and it is dried in the air, fixed on the
flames and stain according to Neisser.
Diphtheria bacilli are located in the smear lean at an angle to each other in the form
of outstretched fingers. The body of the stick turns yellow, and the ends turn
darker; they are called Babesh-Ernst bodies, representing the accumulation of
reserve nutrient - volutin.
Volutin granules
2. Sowing material from the pharynx on Clauberg's medium:
Material for research (thicke only from the pharynx and nose) are taken with
sterile cotton swab. Right after taking material should produce sowing, time
between taking material and sowing should not exceed 5-6 hours.
Diphtheria bacilli grow well on Clauberg's medium will support Na, К tellurium,
Sowing put in a thermostate 370C. After 18-20 hours growth on the surface of the
medium, small colonies appear with a slight compaction in the center or in the
form of large flat colonies with a jagged edge (type gravis) or in the form of round,
convex, shiny colonies with a smooth edge (type gentle). Colonies turn black or
grey-black
Produce in a column of agar with cysteine injection method then test-tube will
be placed in the thermostate at370From at 20-24 h. On the place of injection sein
blackening environment. In presence corynobacterium diphtheria around grow that
a depth of 1 cm from the surface y appears brown "cloud".
1. bacteriological method.
Research material for whooping cough and parapertusis cough is mucus out
deep airway sand excreted by coughing.
For the taking slime swab, without touching the mucous membrane of the
tongue, enter swab over the soft palate and remove the mucus from the back of the
throat. Then seeding is done on Borde-Gengu medium (potato– glycerine before
sowing with a tampon at the surface of the nutrient media with penicillin. Cups
with sowing contain in the thermostate at 370C- 3 days. Colonies of pertussis and
parapertussis rods are smooth, convex, moist, shiny, gray in color, easily removed.
3. Serological method.