Bioinformatics Practical Internal Test

Total Marks: 20 marks

Time: 1 hour
Date: Wednesday 15th February 2023

Question 1. Using NCBI databases, answer the following questions: (Answer the questions
and also paste the screenshots of the database accessed) 6 Marks

a) What is the GC content of DNA and Number of proteins encoded by Staphylococcus

aureus?

Ans:

b) What is the Number of articles published on Klebsiella pneumoniae? Also mention the
total number of free full text article on this organism.

Ans.

Question 2. Retrieve the following sequences (Copy paste the sequence in FASTA format
and also paste the screenshot of the sequence) 4 Marks

a) DNA sequence of tryptophanase gene of Escherichia coli str. K-12 substr. MG1655.

b) Protein sequence of Lipase produced by Bacillus subtilis.

Question 3. Using BLAST, identify the following protein sequence and the producer
organism (Write the name of the organism and paste the screenshot of results) 4 Marks
MFAKRFKTPLLPLFAGFLLLFHPVLAGPAAASAETANKSNELTAPSIKSGTILHAWNWSFNTLKHNMKDI
HDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQPTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAV
INHTTSDYAAISNEVKSIPNWTHGNTQIKNWSDRWDVTQNSLLGLYDWNTQNTQVQSYLKRFLERALNDG
ADGFRFDAAKHIELPDDGSYGSQFWPNITNTAAEFQYGEILQDSASRDAAYANYMNVTASNYGHSIRSAL
KNRNLGVSNISHYASDVSADKLVTWVESHDTYANDDEESTWMSDDDIRLGWAVIASRSGSTPLFFSRPEG
GGNGVRFPGKSQIGDRGSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSS
SVSINTATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVTHSFNDQ
LTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTIMLKGTNSDGVTRTEEYSFIKRDPA
SAKTIGYQNPNHWSQVNAYIYKHDGGQAIELTGSWPGKPMTKNADGIYTLTLPADTDTTNAKVIFNNGSA
QVPGQNQPGFDYVQNGLYNDSGLSGSLPH

Question 4. Using a suitable bioinformatic tool, state how many number of restriction sites
are present on the given DNA sequence. Also mention 3 examples each of blunt end cutters,
sticky end cutters 5’extension and 3’extension (Write the results and paste the screenshot of
the result) 6 Marks
ATGTTTGAAAAACGATTCAAAACCTCTTTACTGCCGTTAGTCGCCGGATTTTTATTGCTGTTTCATTTGG
TTTTGTCAGGGCCTGCGGCTGCAAACGCTGAAACCGCTGACAAATCAAATGAGGTGACCGCATCTTCAGT
CAAAAGCGGAACCATCCTTCATGCCTGGAATTGGTCATTCAATACGTTAACACAAAATATGAAAGATATT
CGTGATGCAGGCTATGCAGCCATTCAGACGTCTCCGATTAACCAAGTAAAGGAAGGGAACCAAGGAGATA
AGAGCATGAGGAATTGGTACTGGCTGTATCAGCCGACATCGTACCAAATCGGCAACCGTTACTTAGGAAC
TGAACAAGAGTTTAAGGACATGTGCGCAGCCGCAGAAAAATATGGCGTAAAAGTCATTGTCGATGCGGTC
ATCAATCATACGACCAGTGATTATAGCGCGATTTCTGACGAGATTAAGCGTATTCCAAACTGGACCCATG
GAAACACACAAATTAAAAATTGGTCGGACAGATGGGATGTCACTCAAAATTCACTATTGGGGCTGTATGA
TTGGAATACTCAAAATACTGAGGTGCAAACCTATCTGAAACGCTTCTTGGAAAGAGCATTGAATGACGGA
GCGGACGGATTCCGTTATGATGCTGCCAAGCATATAGAGCTTCCGGATGACGGGAATTACGGCAGCCGAT
TTTGGCCGAATATCACAAATACAGCGGCGGAATTCCAATACGGAGAAATCCTGCAAGACAGCGCGTCCAG
AGATACTGCTTATGCGAATTATATGAATGTGACCGCTTCAAACTATGGACATTCCATCAGGTCCGCCTTG
AAGAACCGCAATCTGAGTGTGTCGAATATTTCCCATTATGCATCTGATGTGTCTGCGGACAAATTGGTCA
CATGGGTGGAATCACATGATACGTATGCCAATGATGAGGAAGAGTCTACATGGATGAGCGATGACGATAT
CCGTTTAGGCTGGGCAGTGATCGGTTCCCGCTCAGGAAGTACACCTCTTTTCTTTTCCAGACCTGAGGGC
GGAGGAAATGGTGTAAGATTTCCCGGAAAAAGTCAAATAGGCGATCGCGGGAGTGCATTATTTAAAGATC
AAGCGATCACTGCGGTCAATATCTTTCACAATGTAATGGGCGGTCAGCCTGAGGAACTCTCGAATCCGAA
TGGGAACAATCAAATCTTTATGAATCAGCGCGGCTCAAAAGGCGTTGTGCTGGCAAATGCAGGATCGTCT
GCCGTTGCCGTCAATACGTCAACGAAATTACCTGACGGCAGGTATGATAATAGAGCCGGCATCGGTTCAT
TTCAAGTGACGAACGGTAAACTGACAGGCACGATCAATGCCAGATCCGTTGCTGTTCTTTATCCTGACGA
TATCGGAAATGCGCCTCATGTCTTCCTTGAGAATTATCAAACGGGAGCAGTCCATTCTTTCAACGATCAG
CTGACGGTCACCCTGCGTGCAAATGCGAAAACAACAAAAGCCGTTTATCAAATCAATAATGGGCAGCAGA
CGGGATTTAAGGACGGAGACCGATTAACGATCGGAAAAGGAGATCCAATCGGCACGGCATACGACATCAA
GTTAACCGGAACGAACAGCGAGGGTGCAGCAAGAACCCAAGAATACACGTTTGTCAAAAAAGACCCGTCT
CAAGCCAACATCATTGGCTATCAGAATCCGGACCATTGGGGCCAGGTAAATGCTTATATCTATAAACATG
ATGGAGGCAGGGCCATAGAATTAACCGGATCGTGGCCGGGGAAAGCCATGACTAAGAATGCAGATGGAAT
TTACACGCTGACACTGCCTGAGAATGCGGATACGGCCAACGCCAAAGTGATTTTTAACAATGGCAGCGCC
CAAGTGCCCGGACAGAACCAGCCCGGCTTTGATTATGTGAAGAATGGTTTGTATAACAATTCTGGTTTGA
ATGGCTATCTTCGGCATTAA