AGRIC 312- Post-Harvest Handling and Seed Technology

Science and Technology of Seed Production, Processing, Storage,

Testing and Quality control.
LABORATORY ACTIVITY FOR SET A
WEEK 10-17
Introduction
Germination percentage is an estimate of the viability of a population of seeds. The equation to
calculate germination percentage is: GP = seeds germinated/total seeds x 100 . The germination
rate provides an measure of the time course of seed germination.
Plant hormones, mainly abscisic acid (ABA) and gibberellin (GA), are the major endogenous
factors that act antagonistically in the control of seed dormancy and germination; ABA
positively regulates the induction and maintenance of dormancy, while GA enhances
germination.
Gibberellins control different stages of plant development, including seed germination, seedling
growth, stem elongation, root extension, leaf size and shape, flower and fruit development,
pollination (Garcia-Martinez et al., 1997; Yamaguchi, 2008; Heden and Thomas 2012)
Gibberellin is the other major phytohormone with an important role in the regulation of seed
dormancy and germination (Finch-Savage and Leubner-Metzger, 2006).

Worktext in Agri 312- Post-Harvest Handling and Seed Technology

MARCEL B. TOMAS& NORBERT L. RALUSTIAN
Compiler
 AGRIC 312- Post-Harvest Handling and Seed Technology
Science and Technology of Seed Production, Processing, Storage,
Testing and Quality control.
Job sheet no. 4
Title: RICE SEED GERMINATION TESTING
Performance 1. Prepare a demonstration of Seed Germination using Gibberellic Acid
Objectives (GAA3)
2. Measures seed germination and rate
Materials GAA 3, 3 L water, 16 polypropylene box, 2 Filter paper or Tissue paper
Facilities/Area Home/ School
Step and Procedure 1. Prepare 500 grams filled rice seeds (good seeds) and at least 2
liters of clean water.
2. Clean the seeds with tap water thoroughly in two (2) times if
necessary to avoid contamination. Seeds that are floated in the
water should be removed.
3. Air dry the seeds until it is good to transfer into the
polypropylene box. Count 50 seeds per box.
4. Submerge the seeds in 500 ml of water at various GAA 3
Concentration at a different length of time. See table 1: Various
Concentrations at different length of time.
5. Moisten the filter paper or Tissue paper with pure water only.
Spread the seeds on a layer of filter paper or tissue paper in the
polypropylene box.
6. Observe the process of germination for the Monocotyledon seeds.
7. Record the date of planting, breaking the seedcoat and from the
emergence of the radicle and plumule.
8. Determine the germination rate of each treatment as submerge to
different length of time.
9. Make your activity report using the following.
- Observation
- Findings and Result
- Conclusion
- Documentation
10. Complete the data needed in the table 2
11. Submit your Report not later than December 07, 202
Assessment Method Examination. Physical report

Table 1: Various Concentrations level of Gibberellic Acid as expose to different length of

time.
Worktext in Agri 312- Post-Harvest Handling and Seed Technology
MARCEL B. TOMAS& NORBERT L. RALUSTIAN
Compiler
 AGRIC 312- Post-Harvest Handling and Seed Technology
Science and Technology of Seed Production, Processing, Storage,
Testing and Quality control.
TIME (MINUTES)
15 30 45 60
Various a. Control (pure water)
Concentrations of b. 15 ppm (0.015 g/l) A1 A2 A3 A4
Gibberellic Acid c. 25 ppm (0.025 g/l) B1 B2 B3 B4
d. 35 ppm (0.035 g/l) C1 C2 C3 C4
Note: equal level of water at 1L and Boxes should be labeled according to Treatment use

Table 2: Tabulation Result

Gibberellic Total number of Germinated Seeds
Acid at various 15 % 30 % 45 % 60 min %
concentrations min Germinate min Germinate min Germinate Germinate
Control
15 ppm
25 ppm
35 ppm

Materials

Gibberellic Acid (GAA3) Polypropylene box- you can use either

the circle one. The 500 ml can do

Worktext in Agri 312- Post-Harvest Handling and Seed Technology

MARCEL B. TOMAS& NORBERT L. RALUSTIAN
Compiler