Name: Capalungan Queen Bernadeht Date:13/09/2023

Section: BSN-Y1-14 Lab Instructor: John Heart Gavino

Laboratory Report 2: Cellular Structures and Functions

Discuss how to properly use the microscope by recalling your exercise experiences and by
answering the following guide questions:
1. Describe the following objectives: scanner, low power objective, high power objective.
Explain their uses.
= Scanner is the technologies for imaging entire slides that rely on microscopes. Individual
images are captured from each field of vision while the microscope stage moves quickly
and incrementally across the entire microscope slide or sample tray. Next is the lower
power objective or lpo is the several smaller specimens or looking at large specimens since
they have a broad field of view. Lastly is the high power objective or known as hpo deal
for observing fine details within a specimen sample. The total magnification of a high-
power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving
you a very detailed picture of the specimen in your slide.
2. When should someone use the coarse adjustment knob? Fine adjustment knob? What are
their functions?
= We will use coarse adjustment knob and fine adjustment knob when with the lowest
power objective lens. Once it is in focus, you will only need to use the fine focus. Using
the coarse focus with higher lenses may result in crashing the lens into the slide. And the
function of the coarse adjustment and fine adjustment is the coarse adjustment knob quickly
brings the image into focus, and the fine adjustment knobs help maintain focus as the
magnification increases.
3. Is the diameter of field the same when observing the specimen under low power and high
power objective? Explain your answer.
= I think the diameter of the field is not the same when we observing the specimen under
low power and high power objective because the lowest power objective has the largest
field of view. The field of view shrinks towards the center as you increase the power. On
low power, you'll be able to see more of an object. To find an object, center it, then move
to the next higher power and repeat, it is therefore better to start with a low power.
4. Where should the microscope slides be placed? How can we secure the slides from falling
out of the microscope?
= The microscope slides will be placed on the mechanical stage and fasten it with the stage
clips to secure the slides from falling out the microscope
5. What is the purpose of using the stain (methylene blue) in preparing the cheek cell? The
cover slip?
= The purpose of (methylene blue) in preparing the cheek cell it makes the nuclei of the
cells more clear and visible. to hold the specimen in place and protect it from contamination
from the environment.

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Attach the images of your cheek cells observation. Label the images and put a proper figure title.

Theoretical magnification:Scanner Theoretical magnification:LPO Theoretical magnification: HPO

Figure 2.1. HUMAN CHEEK CELL

Draw and label the parts of the cell.

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Fill the table with the needed information. Provide a proper table title.

Table 2.1. _____________________________________________________________________

Organelle Function
selectively- Provide a selective barrier for the cell.
permeable
membrane
Centriole organizing microtubules that serve as the cell's skeletal system .
Mitochondrion produce energy through the process of oxidative phosphorylation
Contains the genetic material
Golgi Apparatus helps process and package proteins and lipid molecules, especially proteins
destined to be exported from the cell.
ribosomes Site of amino acid chain or protein synthesis
Lysosomes Digest foreign particles and worn out parts of the cell
Smooth responsible for the synthesis of essential lipids
Endoplasmic
reticulum
Single, whip-like structure used to propel the cell

Laboratory Performance Rating: ____________