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Service Manual Screenmaster New
Service Manual Screenmaster New
SCREEN MASTER
LIHD113
SERVICE MANUAL
V. 2.0 02/02 ENG
HOSPITEX DIAGNOSTICS
Via S.Piero a Quaracchi, 244 - 50145
Florence Italy
Tel. +39 055 374083 Fax + 39 055 374084
e-mail : hospitex@dada.it
web : http://www.hospitex.com
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Hospitex Diagnostics Screen Master – Service manual
I N D E X:
Page
1. INTRODUCTION ……………………………..………….……………............. 3
3. MEASUREMENT……………. ……………………………………………...... 4
4. INSTALLATION..……………………………………………………………..... 5
4.1 Packing contents……………………………………………………...... 5
4.2 Keyboard……………………………………………………………...... 6
4.3 Rear panel setting…………………………………………………….... 7
4.4 Location of the instrument…………………………………………..... 7
4.5 Power supply connection…………………………………………….... 7
4.6 Reset and switching off……………………………………………….... 8
4.7 Measurement lock settings…………………………………………..... 9
4.8 Tube setting…………………………………………………………...... 9
4.9 Printer settings………………………………………………………..... 10
4.10 Incubator……………………………………………………………...... 11
4.11 Optical group..………………………………………………………..... 11
4.12 amplification…………………………………………………………..... 11
5. SERVICE FUNCTIONS……………………………………………………....... 12
6. INSTALLATION MAP…………………………………………………………. 15
6.1 Connector list………………………………………………………….... 18
6.2 Hydraulics…………………………………………………………......... 19
6.3 Control and calibration……………………………………………….... 20
6.4 Diafram table………………………………………………………….... 22
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Hospitex Diagnostics Screen Master – Service manual
1. INTRODUCTION
It performs:
- End Point
- Kinetic
- Fixed Time
- Multistandard assays
Results are shown on graphic display and printed on paper by in-built printer.
Wavelength 340nm-700nm.
Wavelength selection Automatic via 8-position filter wheel;
6 standard interference filters: 340nm, 405nm, 505nm, 546nm, 578nm, 630nm.
Two free positions for optional filters.
Photometric Range: 0-2.5 O.D.
Flow cell system 32L micro-flow cell with 10mm light path, interchangeable
with 80L micro-flow cell or standard cuvettes (macro or semi-
micro, disposable or special optical glass).
Reading time 1s-300s.
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Hospitex Diagnostics Screen Master – Service manual
Weight Kg 8.5
3. MEASUREMENT PRINCIPLES
The concentration of a solute that absorbs light at a determinate wavelength can be determined with
a spectrophotometer. The fraction of light absorbed is proportional to the concentration of the
absorbing solute (Beer’s low):
(1) I / I0 = e-kc
Where:
I0= Incident light intensity before going through to sample.
I= Transmitted light intensity through to sample.
K= Constant that depends upon the length of light path, nature of the substance and the
light wavelength used.
C= Substance concentration in solution.
log( I0/I) = kc
The quantity log(I0/I) is called Optical Density (OD) or Absorbance (ABS) and is proportional to
concentration.
A spectrophotometer measures the intensity of a known wavelength (measured in nanometers, nm)
of light passed through the sample: OD = log( I0/I)
By using specific reagents in solution for the substance that we want to measure, it is possible to
estimate the constant ( k) for a fixed wavelength, in this way transmitted light intensity becomes
concentration function.
The instrument has a reading point, kept at 25-30-37°C temperature, and in order to perform a
measure it will be necessary to have:
I0 I
receiver A/D
Amplification
Selective filter
and focal system
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4. INSTALLATION
Inside the package you will find a starting kit composed as listed here below, which will allow you
to correctly install the instrument.
If any of the following items is missing or damaged, please contact us for help.
- Power supply cable.
- two fuses.
- Waste bottle and silicone tube.
- One hundred macro cuvette.
- Two paper rolls.
- Instrument cover lining.
- Two cuvette adapters:
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Hospitex Diagnostics Screen Master – Service manual
4.2 Keyboard
Correct use of the keyboard is very important to perform the analyses and to make operations fast
and accurate.
KEY FUNCTIONS
- To insert ‘0’ and to set parameters to zero in ABS mode.
0
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Hospitex Diagnostics Screen Master – Service manual
Located in the rear panel (from left to the right as shown in the picture) you will see:
- Fan for internal cooling (another similar is located under the instrument).
- The power supply switch.
- The polysnap (for the power supply).
- The waste outlet. FAN
POWER
SUPPLY
SWITCH
POLYSNAP
OUTLET
Before switching on the instrument, remember to connect the plastic outlet connector
(blue) to a waste tank by means of a silicone tube.
The instrument should be located in a clean environment, placed on a stable surface, away from dir-
ect sunlight which could affect the operating temperature and the quantity of light measured by the
instrument.
The following points should be taken into consideration.
- Ensure that it is on one level.
- Avoid positions subject to jerks or vibrations.
- Make sure that the instrument is not placed close to air conditioning or heat sources.
- For the long life of the instrument these temperature conditions should be followed.
5°C - 50°C for instrument storage.
15°C - 30°C for instrument use.
- Please check the setting of power supply switch according to your country’s electrical net-
work.
- Connect the power plug to a grounded AC wall outlet, preferably not shared with other
electric appliances and with low fluctuation of the line voltage compared to the standard
voltage specified (10-15%).
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Hospitex Diagnostics Screen Master – Service manual
- Keep the instrument away from other appliances that generate high frequency electrical
noises (e.g. radiological instruments).
- Before connecting the power cord, check that the AC power supply is of the same value as
the one stated on the label stuck on the instrument.
Labels:
For countries with 110 AC voltage supply: For countries with 230 AC voltage supply:
H H
H HOSPITEX DIAGNOSTICS H
HOSPITEX DIAGNOSTICS
Model : SCREEN MASTER Model : SCREEN MASTER
Code : LIHD113 S/N : XXXXXXX Code : LIHD113 S/N : XXXXXXX
AC 110V 50/60 Hz 75W Fuse 4A AC 230V 50/60 Hz 75W Fuse 2A
Made in Italy Made in Italy
Caution
Do not connect to an AC power supply different to the value indicated on the label.
- Before connecting the power and finishing the installation section, make sure that the
instrument is switched off (check the polysnap located on the rear part of the instrument).
- Make sure that your AC main line has an efficient ground line. A bad ground line
connection may compromise analysis results and damage the instrument.
- After switching on the instrument, do not go near the fan with liquids or micro solid
substances.
- Pay attention not to spill liquids and micro solid substances on the surface around the
instrument.
- Keep the instrument away from young children.
If the above procedures are carefully followed up, it will be possible to SWITCH ON the
instrument by using the switch located on the polysnap block.
Remember that the instrument must only be reset if the software does not work.
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Hospitex Diagnostics Screen Master – Service manual
Open the measurement lock cover. It is placed on the left part on the top of the instrument.
You will find:
- a peristaltic pump that allows the solution to enter the instrument for measurement.
- two hollows: one, below, for reading and one for the flow cell service place.
- an incubator which keeps the cuvette at the temperature of approximately 37°C.
- a inlet pipe.
To set the pipe in reading mode, rotate it by about 90 degrees clockwise as displayed in
the figure below, until the tube fits perfectly onto the hole in front of the instrument.
Pump type B (from serial number 1131559): Make sure that the transparent tube (the one that
connects the steel inlet pipe with the internal hydraulic circuit) is not twisted or squashed.
PUMP PUMP
Pump type B
Pump type A
CUVETTE CUVETTE
RED TUBE
POSITION POSITION
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Hospitex Diagnostics Screen Master – Service manual
Under the measurement block there is a lever (the PUSH button). Press the lever for no more than
half a second to pump samples into the flow cell.
MEASUREMENT
BLOCK DOOR
INLET PIPE
PUSH button
GREEN LEVER
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Hospitex Diagnostics Screen Master – Service manual
4.10 Incubator
The incubator temperature is brought up to 37 °C by the software and it will be kept until the
instrument is turned off.
This function is very important to heat macro cuvettes for performing the analysis at the correct
temperature.
Light pulse is performed by an halogen lamp, that covers wavelength range (340630 nm)
with its emission.
Wavelength is selected using optical filters.
Iigth beam is focus on sample by a proper lens system.
Receiver is a photobattery that changes light signal in tension signal.
4.12 Amplification
PREAMP. ON
OPTICAL GROUP
DIFFERENTIAL ON
MAIN BOARD
AMPLIFICATION ON
MAIN BOARD
A/D
Preamplification is performed in a preamp board on optical group, and it has a 12V dinamics.
Differential on main board erases on line noises (giving no further amplification).
Amplification on main board supplies gain over a 5V dinamics.
A/D conveter works on 5V dinamics.
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Hospitex Diagnostics Screen Master – Service manual
5 SERVICE FUNCTIONS
IMPORTANT :
at the same time press several times the following keys depending on which menu you want to
enter:
KEY MENU
Select Italian language
1
2 Select English language
3 Select French language
Temperature Check and adjustment:
4
CUVETTE:
press 1 for 25°C
press 2 for 30°C
press 3 for 37°C
INCUBATOR:
Press 6 for 37°C (fixed temperature)
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Hospitex Diagnostics Screen Master – Service manual
WATCH TENSION
WATCH TENSION
FILTER HOME 1
FILTER 3 4 0
In this screen you can monitor (after having filled water) tension coming from optical group (OPT
TENS) and tension coming in A/D converter (A/D TENS) , using a particular filter (FILTER)
WATCH TENSION SCREEN is very useful to verify lamp calibration , filter / lamp / lens /
photodiode / preamp board quality , position sensor working.
Moreover in this screen you can test correct pump motion.
1…. 340nm
2…. 405nm
3…. 505nm
4…. 546nm
5…. 578nm
6…. 630nm
Press any other 1…6 key if you want to change filter, press PUSH BUTTON if you want to fill
again water.
If you have a correct calibrated optical group, when you use the flow cell, the OPT TENS values
are between 250 mV (for 340 nm) and about 3.5 V (for 630 nm) for all filters.
If you have a correct working of digital potentiometer , you will see A/D TENS value in this range :
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Hospitex Diagnostics Screen Master – Service manual
Each time you set a filter position program is showing you number of step sensor has recognized
wheel crack :
WATCH TEMPERATURE
This screen allows you monitoring temperature gradients of incubator and cuvette according to
temperature displayed in user program and digital level acquired by microcontroller (both current
and target).
As you enter in this menu you will see MEASURE POINT case (cuvette):
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Hospitex Diagnostics Screen Master – Service manual
you will see increasing / decreasing current temperature and digital level towards targets.
when program considered reached target values (with a consequent decrease of temperature
gradient op to zero) software is showing you current values in reverse mode.
6 INSTRUMENT MAP
TRANSFORMER
OPTICAL GROUP
PRINTER
MAIN BOARD
INCUBATOR
DISPLAY
TRANSFORMER
KEYBOARD
EARTH
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Hospitex Diagnostics Screen Master – Service manual
1) 12 – 0 – 12 V for 0. 2 A
2) 7 – 0 – 7 V for 5 A
OPTICAL GROUP
7
3
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Hospitex Diagnostics Screen Master – Service manual
INCUBATOR
PRINTER
DISPLAY
KEYBOARD
MAIN BOARD
Power
Lamp(j1)
Wheel
Earth motor(j4)
connection
12
Pump
motor(j5)
Optical
head(j2) Incubator
Resistors(j17)
Cuvette temp.
Sensor(j19) Peltier
cell(j18)
Incubator
temp.
sensor(j20)
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Index:
1. Power area.
2. Microcontroller area.
3. Memory area.
4. Regulators area.
5. Driver wheel motor area.
6. Driver pump motor area.
7. Driver peltier cell power area.
8. Serial port area.
9. Incubator temperature acquire area.
10. Cuvette temperature acquire area.
11. Optical signal acquire area.
12. Earth connection.
J1 = Lamp.
J2 = Preamplification.
J3 = Trasformer.
J4 = Filter motor.
J5 = Pump motor.
J6 = Position Sensor (OPB).
J7
J8 = Display and keyboard.
J9
J10 = PUSH.
J11
J12
J13
J14
J15
J16 = Data printer.
J17 = Incubator.
J18 = Peltier.
J19 = Temperature sensor cuvette.
J20 = Temperature sensor incubator.
J21 = Power printer.
J22
J23
J24
J25
J26
J27
J28
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Hospitex Diagnostics Screen Master – Service manual
4) Teflon tube AWG 18 with brim towards cuvette part, length cm 40.5.
5) Measure cuvette.
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Hospitex Diagnostics Screen Master – Service manual
1 = 0 V
2 = 7 Vac
3 = 14 Vac
4 = null
5 = null
6 = 12 Vac
7 = 0 Vac
8 = 12 Vac
Tp7 = GND
Tp6 = 12 Vcc
Tp8 = - 12 Vcc
Tp12 = 12 Vcc
Tp14 = 5 Vcc
Lamp tension
Connect Voltmeter Tp10 (GND) and Tp1 and regulate 12 Vcc by RV1
Connect lamp (J1) and control tension again.
Printer tension
Disconnect J15
Connect Voltmeter Tp10 (GND) and Tp9 and regulate 5,6 Vcc by RV5
Connect power printer J15 and control tension again.
Connect Voltmeter Tp10 (GND) and Tp2 and regulate 7.5 Vcc by RV2.
6) Temperature calibration.
Zero offset
-Connect Jp24.
-Connect Voltmeter Tp10(GND) and Tp15 and regulate least tension by RV8.
-Disconnect Jp24.
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Hospitex Diagnostics Screen Master – Service manual
-Connect Jp25.
-Connect Voltmeter Tp10(GND) and Tp17 and regulate least tension by RV10.
-Disconnect Jp25.
Temperartures calibration
Cuvette
-Connect J19.
-Switch on instrument and press 4 times 2 key during chess board on display.
-On menu tes select watch temperature.
-Put termometer on test cuvette test and press 3 (37°), trim digital level 190 by RV7 in
order to have 37.5°C on termometer. When balance is reached the digital level will fluctuate
around 190.
- Switch off instrument.
Incubator
-Connect J20.
-Switch on instrument and press 4 times 2 key during chess board on display.
-On menu test select watch temperature.
-Put termometer on incubator, press OK and 3 (37°), trim digital level 190 by RV9 in
order to have 38.3°C on termometer. When balance is reached the digital level will fluctuate
around 190.
7) Analog calibration.
Offset
- Disconnect J2.
- Connect JP5.
- Connect Voltmeter between TP5 and pin1 of JP6 (near TP4).
- Make zero tension by RV4.
- Disconnect JP5.
- Connect JP6 by a grabbe cable.
- Connect voltmeter between other head of grabber cable and TP4.
- Make zero tension by RV3.
- Disconnect JP6.
Preamplification calibration
- Connect Voltmeter between pin 1 and pin 2 in J2 (extract contacts from connetctor and
connect them at voltmeter).
-Switch on instrument and press 4 times 5 key during chess board on display
-On menu test select watch tension.
- Select 630 nm filter and regulate tension 3,5 Volt by trimmer on preamp board ( using
full of distilled water cuvette ).
- On this table sign up tensions of each filter for at least 3 times.
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Hospitex Diagnostics Screen Master – Service manual
N.B.
For each filter you have to see a value not greater than 3.5 V.
If you find a tension greater than 3.5 V, control if a correct diafram is present, if not, change it and
calibrate preamplification gain.
7. TROUBLESHOOTING
POSSIBLE CAUSES :
POSSIBLE CAUSES :
10 10
10
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Hospitex Diagnostics Screen Master – Service manual
POSSIBLE CAUSES :
POSSIBLE CAUSES :
POSSIBLE CAUSES :
Cases :
1. Wrong ABS values inside significative range (for example little ripples in ABS values):
POSSIBLE CAUSES :
2. Out of significative range (from 3.00 to 5.00) ABS values for every reading session:
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Hospitex Diagnostics Screen Master – Service manual
POSSIBLE CAUSES :
Open case of the instrument, pulling out four screws down the equipment and three ones on the
back side .
Pull out two screws at right and left sides of cuvette site, and move away pump support (the one
with pump rotor).
Move away the little tongue that fix the lamp, by pulling out the only screw .
Extract old lamp, and set carefully the new one, bringing it up to the bottom of the site (pay
attention to not touch lamp glass with finger skin).
Set up again the little tongue, and check the top of the lamp being inside the little hole.
Set up again pump support and equipment case.
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Hospitex Diagnostics Screen Master – Service manual
The red tube of peristaltic pump ( pump type A) does not aspire well.
The white tube of peristaltic pump (pump type B) does not aspire well. To check this operate as
follow:
Switch on the instrument and when chessboard appair press number 7 several times
The following screen will appair:
5100 1150
Cuvette hose
Peristaltic pump:
1. Red tube (type A)
2. white tube (type B)
Manual
cuvette 25
Flow cell
Hospitex Diagnostics Screen Master – Service manual
If water stops at beginning of aspiration tip or stops to near the cuvette the red tube has a problem
and you have to adjust aspiration by changing the aspiration pump steps.
proceed as follow:
Press up arrow keys to increase aspiration pump steps (the liquid will stop nearer to cuvette)
Press down arrow keys to decrese aspiration pump steps(the liquid will stop further to
cuvette)
Prepare 500 l of water in a cuvette set the aspiration tip in an bottom angle of manual
cuvette and press (.) key to aspire
If the pump works correctly, the water should be aspired completely from the manual
cuvette, and also the aspiration tip should be empty from water:
Keep adjusting until the aspiration is correct
1. Disconnect the red tube from peristaltic pump by pulling one of the white holder.
2. Free the red tube from holders by pulling the two white pieces apart:
3. Measure the red tube with a ruler, the length must be 10 cm, if not cut the exceeding of the
tube with sharp cutter and set the tube back. Then check aspiration as appendix 2.
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