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MCQ Biotechnology CRT Questions
MCQ Biotechnology CRT Questions
MCQ Biotechnology CRT Questions
1. Which of the following is the most commonly used carbon source in Plant tissue culture
media?
(a) Agar
(b) Sucrose
(c) Kinetin
(d) EDTA
2. Higher concentration of which hormone promotes shoot induction in Plant tissue culture
(a) Sucrose
(b) Inositol
(c) Auxins
(d) Cytokinins
3. The culture of tissues and cells cultured in a liquid nutrient medium produce single cells
and cell clumps, this is called
(a) Callus culture
(b) Meristem culture
(c) Tissue culture
(d) Suspension culture
5. Which of the following form the most successful plant transformation system
(a) Particles Bombardment method
(b) Direct gene transfer
(c) Agrobacterium mediated gene transfer
(d) Electroporation
7. Bacillus thuringiensis is a
(a) Bacteria
(b) Fungi
(c) Mold
(d) Cotton
23. In plant tissue culture, the callus tissues can be regenerated into complete plantlets
primarily by altering the concentration of
(a) Sugars
(b) Vitamins
(c) Amino acids
(d) Hormones
25. The problem of necrosis and gradual senescence while performing tissue culture can be
overcome by
(a) Spraying auxins
(b) Spraying cytokinins
(c) Suspension culture
(d) Subculture
26. Somaclonal variation appears in plants
(a) Growing in polluted soil or water
(b) Exposed to gamma rays
(c) Raised in tissue culture
(d) Transformed by recombinant DNA technology
27. The ability of the component cells of callus to form a whole plant is known as
(a) Redifferentaition
(b) Dedifferentiation
(c) Either (a) or (b)
(d) None of these
29. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by
enzymatic treatment with
(a) Cellulolytic enzymes
(b) Pectolytic enzymes
(c) Both Cellulolytic and Pectolytic enzymes
(d) Proteolytic enzymes
34. The mechanism which has the ability to engineer new organisms is known as
(a) Totipotency
(b) Molecular cloning
(c) Genetic Engineering
(d) Splicing
36. Golden rice is a transgenic crop of the future with the following improved trait.
(a) Insect resistance
(b) High protein content
(c) High vitamin A content
(d) High lysine content
37. The technique of obtaining large number of plantlet by tissue culture method is called
(a) Plantlet culture
(b) Micropropagation
(c) Organ culture
(d) Macro propagation
38. Maximum application of animal cell culture technology today is in the production of
(a) Insulin
(b) Interferons
(c) Edible proteins
(d) Vaccines
39. Two bacteria found to be very useful in genetic engineering experiments are
(a) Nitrobacter and Azotobacter
(b) Rhizobium and Diplococcus
(c) Nitrosomonas and Klebsiella
(d) Escherichia and Agrobacterium
47. The unpaired nucleotides produced by the action of restriction enzymes are referred to
have
(a) Sticky ends
(b) Single strands
(c) Restriction fragments
(d) Ligases
49. The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-
causing bacteria.
(a) Would be of no concern if it occurred.
(b) Has occurred
(c) Can never occur
(d) Seems unlikely
51. In first step of genetic engineering, special enzymes that are used to separate identified
gene are called
(a) Expressed nucleases
(b) Inserted nucleases
(c) Restriction end nucleases
(d) Isolated nucleases
52. Particular enzyme produced by genetic engineering which is used to dissolve clots of
blood is called
(a) Interferon
(b) Urokinase
(c) Thymosin
(d) Beta-endorphin
54. Cutting certain genes out of molecules of DNA requires the use of special
(a) Degrading nucleases.
(b) Restriction endonucleases.
(c) Eukaryotic enzymes.
(d) Viral enzymes.
61. Process of manipulating genes usually outside normal reproductive process is known as
(a) Genetic modification
(b) Gene targeting
(c) Genome recombination
(d) Gene linking
65. The first successful transformation of rDNA molecule into a bacterium was carried out by
(a) Nathan, Arber and Smith
(b) Watson, Crick and Wilkins
(c) Boyer and Cohen
(d) Paul Berg
66. The DNA molecule to which the gene of insert is integrated for cloning is called
(a) Carrier
(b) Vector
(c) Transformer
(d) None of these
67. The most important discovery that lead to the development of rDNA technology was
(a) Double helix model of Watson and Crick
(b) Discovery of ligase enzyme
(c) Discovery of plasmids
(d) Discovery of restriction enzymes
68. The mechanism of intake of DNA fragments from the surrounding medium by a cell is
called
(a) Transformation
(b) Transduction
(c) both a and b
(d) conjugation
70. Which of the following enzyme is used to cut DNA molecule in rDNA technology
(a) Restriction enzymes
(b) Ligase
(c) Phosphatase
(d) Ribonuclease
72. Which of the technologies listed below is a valuable method for mass-producing drugs
and other useful proteins?
(a) Recombinant DNA technology
(b) Transgenic technology
(c) Biotechnology
(d) Gene targeting
73. Which gene transfer technique involves a tiny needle which is used to inject DNA into a
cell lacking that DNA sequence?
(a) Electroporation
(b) Liposome transfer
(c) Microinjection
(d) Particle bombardment
78. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
(a) In both cases molecules migrate to the anode
(b) Both techniques rely on a constant charge to mass ratio
(c) Both techniques utilize the sieving properties of gels
(d) All of the above
79. In order to insert a foreign gene into a plasmid, both must __________
(a) Have identical DNA sequences
(b) Originate from the same type of cell
(c) Be cut by the same restriction enzyme
(d) Be of the same length
80. The gene formed by the joining of DNA segments from two different sources are called
as
(a) Recombinant gene
(b) Joined gene
(c) Both a and b
(d) Chimeric gene
84. All the terminator codon begins with the nucleotide of-
(a) Uracil
(b) Adenine
(c) Guanine
(d) Cytosine
87. UGA, UAG and UAA are called termination codons because they-
(a) Terminate anticodon
(b) Do not specify any amino acid
(c) Present at the beginning of m-RNA
(d) Indicate initiation of polypeptide chain
93. Which of the following inactivates the 60S subunit of eukaryotic ribosomes?
(a) Chloramphenicol
(b) Cycloheximide
(c) Diphtheria toxin
(d) Ricin
98. Amino acids are joined together into a protein chain by which of the following?
(a) Transfer RNA
(b) DNA polymerase
(c) Hydrogen bonds
(d) Messenger RNA
102. The pathway of a tRNA during polypeptide elongation on the ribosome is-
(a) A site → P site → E site
(b) P site → entry site → exit site
(c) A site → P site → entry site
(d) P site → A site → E site
103. Which of the following amino acid starts all proteins synthesis?
(a) Glycine
(b) Proline
(c) Thymine
(d) Methionine
104. Peptide bond formation between amino acids of growing polypeptide chain is catalysed
by?
(a) Peptide polymerase
(b) Amino acyl-tRNA synthetase
(c) Peptidyl transferase
(d) Peptidyl synthetase
110-The process by which mRNA is made by DNA and protein by mRNA are respectively
called?
(a) Translation and Transcription
(b) Transcription and Translation
(c) Synthesis of mRNA and protein
(d) Replication of mRNA and protein
120- Which of the following conditions leads to maximal expression of the lac operon?
(a) Lactose present, glucose absent
(b) Lactose present, glucose present
(c) Lactose absent, glucose absent
(d) Lactose absent, glucose present
121- Which of the following is a type of regulation of gene expression unique to eukaryotes?
(a) Attenuation
(b) Use of alternate σ factor
(c) Alarmones
(d) Chemical modification of histones
128-When more than one codon code for the same amino acid, this is called as?
(a) Universal nature of genetic code
(b) Redundancy of genetic code
(c) Punctuation in genetic code
(d) Continuous nature of genetic code
130-Genes that are involved in turning on or off the transcription of a set of structural genes
are called?
(a) Polymorphic genes
(b) Operator genes
(c) Redundant genes
(d) Regulatory genes
133-Which one of the following codons codes for the same information as UGC?
(a) UGU
(b) UGA
(c) UAG
(d) UGG
134- In the lactose operon of Escherichia coli, what is the function of promoter?
(a) Binding of Gyrase enzyme
(b) Binding of RNA polymerase
(c) Codes for RNA polymerase
(d) Processing of messenger RNA
Answer key:
1. (b), 2. (d), 3. (d), 4. (a), 5. (c), 6. (c), 7. (a), 8. (c), 9. (b), 10. (d), 11. (c), 12. (b), 13. (b), 14.
(a), 15. (b), 16. (d), 17. (b), 18. (b), 19. (c), 20. (d), 21. (a), 22. (a), 23. (d), 24. (c), 25. (b), 26.
(c), 27. (a), 28. (c), 29. (c), 30. (d), 31. (a), 32. (b), 33. (c), 34. (c), 35. (b), 36. (c), 37. (b), 38.
(d), 39. (d), 40. (d), 41. (a), 42. (c), 43. (a), 44. (b) 45-(b), 46-(d), 47-(a), 48-(d), 49-(d), 50-
(a), 51-(c), 52-(b), 53-(d), 54-(b), 55-(c), 56-(d), 57-(c), 58-(d), 59-(b), 60-(b), 61-(a), 62-(d),
63-(d), 64-(b), 65-(c), 66-(b), 67-(d), 68-(a), 69-(c), 70-(a), 71-(d), 72-(a), 73-(c), 74-(a), 75-
(b), 76-(d), 77-(a), 78-(d), 79-(c), 80-(d), 81-(a), 82-(b), 83-(c), 84-(a), 85-(d), 86-(c), 87-(b),
88-(d),89-(a), 90-(c), 91-(a), 92-(c), 93-(d), 94-(c), 95-(b), 96-(d), 97-(c), 98-(a), 99-(b), 100-
(a), 101-(d), 102-(a), 103-(d), 104-(c), 105-(d), 106-(c), 107-(b), 108-(a), 109-(c), 110-(b),
111-(c), 112-(a), 113-(d), 114-(b), 115-(d), 116-(a), 117-(c), 118-(b), 119-(c), 120-(a), 121-(d),
122-(b), 123-(c), 124-(d), 125-(b), 126-(c), 127-(a), 128-(b), 129-(a), 130-(b), 131-(a), 132-
(d), 133-(a), 134-(b), 135-(c), 136-(b), 137-(a), 138-(b), 139-(d), 140-(b), 141-(a), 142-(d),