Laboratory Report No.

3
Separation of Constituents of Mixtures through Centrifugation
submitted by: Carmina S. Villaruel
BSEd Science III
Eastern Samar State University-Salcedo Campus
Carminavillaruel2000@gmail.com

submitted by: Rica C. Elacion

Assistant Professor
Eastern Samar State University-Salcedo Campus
rica.c.elacion@gmail.com
Date submitted: September 12, 2023

The separation of a mixture into its pure
constituents by the usual methods such as
fractional distillation, fractional crystallization,
etc., sometimes is a very slow and tedious
process. This is especially true in the case of
many constant boiling point mixtures.
Therefore, it might be of interest to mention
some experiments in which our vacuum type
tubular centrifuge was used to separate a
mixture of sub-stances of different molecular
weights while in the vapor.
Fig. 1 Example of Low Speed Centrifuge
In a solution, particles whose density is higher
than that of the solvent sink (sediment), and
particles that are lighter than it floats to the top.
The greater the difference in density, the faster
they move. If there is no difference in density
(isopycnic conditions), the particles stay
steady.To take advantage of even tiny
differences in density toseparate various
particles in a solution, gravity can bereplaced
with the much more powerful
“centrifugalforce” provided by a centrifuge. A
centrifuge is a piece of equipment that puts an
objectin rotation around a fixed axis (spins it in
a circle),applying a potentially strong force
perpendicular to theaxis of spin (outward).
This paper presents a different sample mixture
that is being centrifuge in a low speed
centrifuge. This different mixture is rotated in
the centrifuge with different time allotted on
every group of samples.
Documented in this paper on how we conduct
our experiment in centrifugation and how
manage to use the low speed centrifugation in
different period of time, and also the result of
our experiment is been documented in this
paper. The activity presented on this After conducting the experiment clean the table
experiment can give students an insight on how and the materials that you use in the
to use or handle the centrifuge and explore on experiment, separate the disposal garbage and
how different solution can separate its particles the materials that are need cleaning. Return the
to its liquid materials. materials to its proper place and don’t leave the
table dirty. Wash your hand and clean yourself
Materials: before leaving the laboratory.
• Centrifuge Result and Discussion:
• Goggles
• Beaker By following the procedure of the
• Centrifugal tube experiment in separating constituents of
• Milk mixtures from different sample, we obtain
• Orange Juice this result.
• Pineapple juice
• Blood
Orange Juice
• Muddy water
Time Speed Documentation
•
Procedure:
Satisfactory in separating mixtures through
centrifugation technique might be successful in
3 minutes 1200 rpm
following the procedure that is provided.
Prepare the materials needed for the experiment
(2) Prepare the sample and put small amount of
it in every centrifugal tube. (3) Make sure that
every tube has same volume . (4) Open the
centrifuge and place the tube with samples,
place it facing each other samples. (5) make
sure that the position of every sample is balance
so that the centrifuge work well. (6) Set the time
and speed you want to set to achieve the desired
result. (7) Wait for the centrifuge to finish its 5 minutes 1200 rpm
rotation, if it stop you may now get the tubes
and observe. (8) observe the sample and the
result before and after it place in centrifuge. (9)
Write your observation in your notes.

Safety Precaution:
In doing different experiment everyone should
Table 1: Show the result of orange juice
always follow the safety precautions, student
sample after being centrifuge, with 3 minutes
must always wear the PPE in conducting
time and 5 minutes with a speed of 1200 rpm.
laboratories, wearing; laboratory gown and
gloves is a must to avoid contact in different
chemicals and other hazardous substance. Wear
face mask and practice etiquette in using
laboratory equipment. Label the materials
especially the chemical that you use in the
experiment.
 Pineapple Juice Mil
Time Speed Documentation Time Speed Documentation

3 minutes 1200 rpm

3 minutes 1200 rpm

5 minutes 1200 rpm

5 minutes 1200 rpm

Table 3: Show the result of milk sample after

Table 2: Show the result of pineapple sample being centrifuge, with 3 minutes time and 5
after being centrifuge, with 3 minutes time minutes with a speed of 1200 rpm.
and 5 minutes with a speed of 1200 rpm.

Blood Mil
Time Speed Documentation Time Speed Documentation

3 minutes 1200 rpm 3 minutes 1200 rpm

5 minutes 1200 rpm 5 minutes 1200 rpm

Table 3: Show the result of blood sample after Table 3: Show the result of muddy water
being centrifuge, with 3 minutes time and 5 sample after being centrifuge, with 3 minutes
minutes with a speed of 1200 rpm. time and 5 minutes with a speed of 1200 rpm.
The tables show the different mixture http://www.phys.virginia.edu/History/Beams/
sample that are tested and centrifuged in a Papers/Beams%201937e.pdf, September 10,
Low Speed Centrifuge. The first column in 2023.
each table shows the time of every samples. 2. Moreno, J. (2000). Centrifugation
Next column is the speed of every sample Retrieved from :
while inside in the centrifuge, the last
column shows the result or the documented http://www.rdscollege.ac.in/studymaterial/15
result of each samples. Every sample is 96509081.pdf?uid September 10, 2023
tested in centrifuge in different time 3. Heip C. (2010). Centrifugation Method
allotted, and the result varies depending the Retrieved from:
speed and the content of every ample.
https://www.flottweg.com/fileadmin/user_up
Generalization load/data/pdf-downloads/Bohrschlamm-
EN.pdf September 10, 2023
In this laboratory experiment, we
conducted about the separation of Appendices:
constituents of mixture through
centrifugation. This technique is useful in
separating two miscible substances, and an
effective assisted technique to remove the
concentrated solution from different
mixtures. This technique is useful
especially in clinical or scientific usage in
separating solid substance from a liquid.
From this experiment, we have an insight Fig. 2 Some of the materials and sample
on how centrifuge work on separating mixture
mixtures of any substance, gas can also
separate by centrifugation that is why this
technique is use even in engineering but in
high-speed type of centrifuge.

In conclusion, separation of constituents of

mixture through centrifugation has a vital
importance in the field of science, and this
applied in different application, medicinal, Fig. 3 Low speed Centrifuge
engineering and so on. The result of this Fig. 1 Example of Low Speed Centrifuge
technique may vary depending of the first,
sample mixture, and second the type of
centrifuge, third the time and speed allotted on
Example of Low Speed Centrifuge
every samples.

References:
1. Beams, W., Snoddy, L. (2012). The
separation of Mixtures by Cetrifuging Fig. 4 sample mixture after undergoing
Retrieved from: centrifugation
Fig. 1 Example of Low Speed Centrifuge

Example of Low Speed Centrifuge