OLIVAREZ COLLEGE TAGAYTAY

PROGRAM: BACHELOR OF SCIENCE IN NURSING

A.Y. 2023 - 2024 1st
SEMESTER
Name: ___________________________________________ Date: _________________________
Section: __________________________________________ Grade: ________________________

Instruction: Print this worksheet on or before the scheduled activity date.

Worksheet 2
Laboratory Activity for Week 3: Viewing Plant and Animal Cells under the microscope.

o You are tasked to prepare, preserve, and mount microscopic specimens from plant and animal parts to be
observed under the microscope.

Materials:

Compound Microscope 1 Dropper Tissue

6 Slides and Coverslip 1 Forceps Balsam
1 Razor Blade 1 Beaker Transparent Nail Polish
Wright Stain Xylene Methylene Blue/ Saffron

Execution of how to prepare slide with dry mounting, wet mounting, squashing, and staining
 Dry mount – fixing a dry specimen at the top of the slide with a cover slip
 Wet mount – adding a drop of oil or water at the top of the slide
 Squashing
1. Place a drop of water on the slide and then a small piece of the specimen into the water.
2. Carefully position the specimen in the center and place the cover glass on top, as if making a
regular wet mount.
3. Using a soft round object, such as an eraser, carefully press down on the coverslip without
horizontal movement, which would introduce shearing forces. This is the testing stage, to check if
the specimen is sufficiently soft. The cover glass may break otherwise. This is, why you should use
an eraser, to protect yourself.
4. If the sample is sufficiently soft, you can press down with more force. The sample should form a
thin, almost transparent film between coverslip and slide. Again, do not introduce a horizontal
movement.
5. Excess water should be soaked up with tissue paper. If some of the specimen starts to appear
from beneath the cover glass, then you used too much specimen.
 Staining
1. Positive Staining
 Gram Positive
 Apply Crystal Violet then mordant iodine
2. Negative Staining
 Gram Negative
 Lose the stain with alcohol then stain with safranin

Procedure:

I. Obtain the samples and follow the procedure on how to prepare the sample.

PLANT SAMPLES ANIMAL SAMPLES

Pollen Grain Small Arthropod (Insect: ant, lice, fruit fly, mosquito)

1. Obtain an anther with pollen grains from a flower. 1. Place a dead small arthropod (insect) in the middle
2. Place it in the middle of the slide. of the slide.

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 OLIVAREZ COLLEGE TAGAYTAY
PROGRAM: BACHELOR OF SCIENCE IN NURSING
A.Y. 2023 - 2024 1st
SEMESTER
3. Pour enough balsam/ nail polish and cover it with a 2. Add a few drops of water and cover it with a
coverslip. coverslip.
4. Carefully press the cover slip against the pollen. 3. Observe under the microscope to check the quality.
5. Observe under the microscope to check the quality. 4. Once you obtain good samples, mount in balsam
6. Observe under the microscope for illustration. and coverslip.
5. Observe under the microscope for illustration.

Onion Epidermis Liver Prints

1. Peel an onion bulb. Cut it into two parts.
2. Peel a thin section of the epidermis of the onion.
3. Cut a 1.0 x 0.5 cm of the epidermis and place it on
the slide.
4. Add drops of iodine solution until the nucleus of the
cells become visible.
5. Air-dry the specimen.
6. Clean excess stain using xylene.
7. Observe under the microscope to check quality.
8. Once you obtain good samples, mount in balsam
and coverslip.
9. Observe under the microscope for illustration.
1. Obtain raw pork liver. Cut it to 1x1x1 cm or 1 cm 3.
2. Using forceps, let the liver touch the surface of the
slide leaving a mark on it.
3. Air-dry the liver print left.
4. Add drops of methylene blue.
5. Tilt the slide by holding one of its ends.
6. Carefully drop water on the elevated part of the
slide removing excess stain.
7. Air-dry the specimen.
8. Observe under the microscope to check the quality.
9. Once you obtain good samples, mount in balsam
and coverslip.
10. Observe under the microscope for illustration.

II. Illustrate the images you have observed under the microscope.
III. Label the parts of the specimen if they are familiar to you. Indicate the magnification power.

PLANT SPECIMEN

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 OLIVAREZ COLLEGE TAGAYTAY
PROGRAM: BACHELOR OF SCIENCE IN NURSING
A.Y. 2023 - 2024 1st
SEMESTER
ANIMAL SPECIMEN

IV. Write a reflection journal about your difficulties and learnings from this activity. Reflective essay should be
composed of Introduction, Body and Conclusion.

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