3.

Briefly explain the importance of equilibrating the developing chamber before

chromatography. Ready to be Transferred
It's important to do chamber equilibration to help the vapor from the mobile phase spread
out evenly in the chamber. This speeds up the “development” or the separation of
components between the mobile and stationary phases and also helps avoid any issues
with getting the same results every time. Getting the chamber equilibrated or "saturated"
is really important to consistently get the same Rf values.

4. What colored pigments were extracted from malunggay leaves, kindly draw the structure
for each. From the pigment structures, identify which is the most and least polar. Ready to be
Transferred

Pigment Structure Polarity (Rank: 1 is most polar; 8 is least

Extracted polar)

Orange

Orange

Yellow

Yellow 0.71

Dark green Rf value = 0.59

Lime green Rf value = 0.42

1
Dark gray 4

Bluish Gray 3