V13 - 00 Technical Manual H18-Ant2

Revision History
Version Date Modification

V7.00 02/03/2009 Initial release
V12.00 04.2012 Total redesign
V13.00 09/12/2013 Added Appendix 5, User Maintenance Schedule
H18 Light V2_Service Manual_English_V13.00 | 3

Table of contents
1. SPECIFICATIONS......................................................................... 15
1.1. FRONT FACE VIEW 15
1.2. BACK PANEL VIEW 16
1.3. SYSTEM SPECIFICATIONS 17
1.3.1. PARAMETERS 17
1.3.2. VOLUMES 18
1.3.3. DILUTION 18
1.3.4. INPUT/OUTPUT 18
1.3.5. PRINTOUT 18
1.3.6. LINEARITY AND PRECISION 18
2. INSTALLATION PROCEDURE ....................................................... 19

2.1. PRE-INSTALLATION ON SITE INSPECTION 19
2.1.1. SPACE REQUIREMENTS 19
2.1.2. LOCATION REQUIREMENTS 19
2.1.3. POWER REQUIREMENTS 19
2.2. PRE-INSTALLATION INSTRUMENT INSPECTION 19
2.3. UNPACKING / INSTALLING THE H18 LIGHT 19
2.4. PERFORMANCES VERIFICATION 23
2.4.1. BACKGROUND 23
2.4.2. STABILITY : CV 23
2.4.3. CALIBRATION 24
3. THEORY OF OPERATION ............................................................ 25

3.1. COUNTING BLOOD CELLS 25
3.1.1. COULTER PRINCIPAL 25
3.1.2. TREATING THE SIGNAL 26
3.2. HGB READING 26
4. FLUIDICS SYSTEM ........................................................................ 27

4.1. FLUIDICS PHASES 27
4.1.1. STAND BY 27
4.1.2. DILUTION 27
4.1.3. COUNTING 27
4.1.4. CLEANING 27
4.2. FLUIDIC ALGORYTHM 29
4.3. FLUIDIC SCHEMATIC 30
4.4. FLUIDICS COMPONENTS 31
4.4.1. RBC CHAMBER : ITEM CODE S020010 32
4.4.2. WBC CHAMBER 32
4.4.3. DILUTION BLOCK 33
4.4.4. VACUUM BLOCK 35
4.4.5. SAMPLE PROBE WASHER 36
4.4.6. TWO WAYS AND THREE WAYS VALVES 36
4.4.7. FLUIDICS PARTS LIST 31
5. ELECTRONICS ............................................................................. 39

5.1. SWITCHING POWER SUPPLY 39
5.1.1. ITEM CODE: S020056-V2 39
5.1.2. FUNCTIONS 39
5.1.3. NOTE 39
5.2. CENTRAL COMMAND UNIT, CCU BOARD 39
5.2.1. ITEM CODE: S020003-02 39
5.2.2. FUNCTIONS 39
5.2.3. FUNCTIONAL SCHEM 42
5.2.4. COMPONENTS LAYOUT 43
5.2.5. CONNECTORS’ DESCRIPTION TABLE 45
5.2.6. TEST POINTS TABLE 46
5.3. CENTRAL PROCESSING UNIT: CPU BOARD 47
5.3.1. ITEM CODE: S020001-02 47
5.3.2. FUNCTIONS: 47
5.4. LIQUID SENSOR ADJ. /CONNECTION DISTRIBUTION BOARD 48
5.4.1. ITEM CODE: S020063-02 48
5.4.2. FUNCTIONS: 48
5.5. LCD ADAPTOR BOARD 49
5.5.1. ITEM CODE: S020067 49
5.5.2. FUNCTION: 49
5.6. ON BOARD THERMAL PRINTER DRIVER BOARD 49
5.6.1. ITEM CODE: S020028 (COMPLETE ASSEMBLY, INCLUDED PRINTER HEAD) 49
6. MECHANICS ............................................................................... 50
6.1. SAMPLE PROBE ARM 50
6.1.1. ITEM CODE: S020062-02 50
6.1.2. FUNCTION 50
6.2. DILUTION MACHINE 51
6.2.1. ITEM CODE: S020023 51
6.3. VACUUM PUMP MACHINE 52
6.3.1. ITEM CODE: S020064 52
7. SERVICE SOFTWARE ................................................................... 53

7.1. MECHANICS 53
7.1.1. ELECTRONIC VALVE: 54
7.1.2. MOTOR 54
7.1.3. CIRCUIT 55
7.2. SPECIAL 56
7.2.1. FACTORY MODE 56
7.2.2. PARAMETERS SCREEN 56
7.3. NETWORK TRANSFERT PROTOCOL 58
7.3.1. INTRODUCTION 58
7.3.2. TRANSFER PROTOCOL 58
7.3.3. WIRING 58
7.3.4. DATA BLOCK FORMAT 58
7.3.5. USAGE OF WBC, RBC, PLT HISTOGRAM DATA 59
7.3.6. EXAMPLE 59
7.3.7. HARDWARE TO TEST COMMUNICATION 61
8. ADJUSTMENTS AND CONTROLS ................................................ 64

8.1. REAGENT ALARM TEST 64
8.2. COOLING FAN TEST 65
8.3. VALVES COMMUTATION TEST 65
6 | H18 Light V2_Service Manual_English_V13.00

8.4. MICROCONTROLER TEST 66
8.4.1. A/D CONVERSION 66
8.4.2. INT 66
8.5. POWER SUPPLY: 5VDC 66
8.6. HGB BLANK VOLTAGE 66
8.7. ACCURATE COUNTING VACUUM CHECK 68
8.8. BURNING FUNCTION CHECK 69
8.9. DILUENT TEMPERATURE CHECK 69
8.10. SPEAKER CHECK 69
8.11. APERTURES VOLTAGES CHECK 70
8.12. MOTORS CHECK 70
8.13. DILUENT DISPENSING CHECK 70
8.14. REAGENT SENSORS SENSITIVITY CHECK 70
8.15. CHANNELS GAIN ADJUSTMENT 70
8.16. APERTURE BASELINE VOLTAGE SETTING 70
8.16.1. ENTER INTO FACTORY MODE 70
8.16.2. BLANK TEST 70
8.16.3. CHANNEL DATA MONITOR 71
8.16.4. APERTURE BASELINE VOLTAGE SETTING 71
9. MAINTENANCE ........................................................................... 72
9.1. USER MAINTENANCE 72
9.1.1. DAILY MAINTENANCE 72
9.1.2. WEEKLY MAINTENANCE 72
9.1.3. ON DEMAND MAINTENANCE 72
9.1.4. MAINTENANCE LOG 73
9.2. SFRI TECHNICAL MAINTENANCE 75
9.2.1. PREVENTIVE MAINTENANCE PROCEDURE 75
10. Troubleshooting ......................................................................... 85
Appendices – TSB ............................................................................... 87

APPENDIX 1: SAFETY SYMBOLS 89
APPENDIX 2: PREVENTIVE MAINTENANCE CHECK LIST 91
APPENDIX 3: PASSWORDS 94
APPENDIX 4: THE FLUIDIC CYCLE STEP BY STEP 95
APPENDIX 5: USER MAINTENANCE SCHEDULE SHEET 110
APPENDIX 6: SPARE PARTS LIST 111

Statement
SFRI Sarl owns all rights to this unpublished work and intends to maintain this work as confidential.
SFRI Sarl may also seek to maintain this work as an unpublished copyright. This publication is to be
used solely for the purposes of reference, operation, maintenance, or repair of SFRI Sarl equipment.
No part of this can be disseminated for other purposes.
In the event of inadvertent or deliberate publication, SFRI Sarl intends to enforce its rights to this work
under copyright laws as a published work. Those having access to this work may not copy, use, or
disclose the information in this work unless expressly authorized by SFRI Sarl to do so.
All information contained in this publication is believed to be correct. SFRI Sarl shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the furnishing,
performance, or use of this material. This publication may refer to information and protected by
copyrights or patents and does not convey any license under the patent rights of SFRI Sarl, nor the
rights of others. SFRI Sarl does not assume any liability arising out of any infringements of patents or
other rights of third parties.
Content of this manual is subject to changes without prior notice.
Responsibility on the Manufacturer Party

SFRI Sarl is responsible for safety, reliability and performance of this equipment only in the condition
that:
· All installation, expansion, change, modification and repair of this equipment are conducted
by SFRI Sarl qualified personnel;
· Applied electrical appliance is in compliance with relevant National Standards;
· The analyzer is operated under strict observance of this manual.
Warning
For continued safe use of this equipment, it is necessary that the listed instructions are followed.
However, instructions listed in this manual in no way supersede established medical practices
concerning patient care.
· This equipment is intended for use only by medical professionals in health care institutions.
· To avoid electrical shock, you should not open any cover by yourself. Service must be carried
out by qualified personnel only.
· It is dangerous to expose electrical contact or applicant coupler to normal saline, other liquid
or conductive adhesive. Electrical contact and coupler such as cable connector, power supply
must be kept clean and dry. Once being polluted by liquid, they must be thoroughly dried. If
to further remove the pollution, please contact your biomedical department or SFRI Sarl.
It is important for the hospital or organization that employs this equipment to carry out a reasonable
maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.
Upon request, SFRI Sarl may provide, with compensation, necessary circuit diagrams, calibration
illustration list and other information to help qualified technician to maintain and repair some parts,
which SFRI Sarl may define as user serviceable.

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.
Workmanship and Materials

SFRI Sarl guarantees new equipment other than accessories to be free from defects in workmanship
and materials for a period of one year from date of shipment under normal use and service. SFRI Sarl's
obligation under this warranty is limited to repairing, at SFRI Sarl’s option, any part which upon SFRI
Sarl's examination proves defective.
Exemptions
SFRI Sarl's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the improper
use or application of the product or the substitution upon it of parts or accessories not approved by
SFRI Sarl or repaired by anyone other than a SFRI Sarl authorized representative.
This warranty shall not extend to any instrument which has been subjected to misuse, negligence or
accident; any instrument from which SFRI Sarl's original serial number tag or product identification
markings have been altered or removed, or any product of any other manufacturer.
Safety, Reliability and Performance

SFRI Sarl is not responsible for the effects on safety, reliability and performance of the H18 Light if:
· Assembly operations, extensions, re-adjusts, modifications or repairs are carried out by
persons other than those authorized by SFRI Sarl.
· Personal unauthorized by SFRI Sarl repairs or modifies the instrument.
Warning
The H18 Light is an In Vitro Diagnostic instrument. It may indirectly bring risks to patients and present
a direct risk to operators and the environment during manipulation. To avoid these risks, be sure to
read the following instructions carefully.
This device uses AC 220V alternating courant. Do not open the rear cover before switching
off the power supply. If you need to open the rear cover of the device for maintenance
reasons, please contact our service personnel or professionals.
Patient samples are potentially contaminating agents. Manipulate with care using
appropriate protection equipment (glasses, gloves and laboratory coat) and be sure to
dispose of the waste in accordance with relevant regulations of your hospital.
Do not operate the device unless you have read this Operation Manual carefully

Company Contacts (Manufacturer)
Address: SFRI Sarl
Lieu-dit Berganton
33127 SAINT JEAN D’ILLAC
FRANCE
Phone: +33 (0) 556 688 050
Fax: +33 (0) 556 217 903
E-mail : support@sfri.com

About this Service Manual
This Service Manual, in addition to the training courses run by SFRI Sarl FRANCE, is the basis for the
maintenance and repair of your 3 Part Diff Cell Counter H18 Light Version 2 (H18 Light V2, is H18 Light
with serial number above 02010411). This manual is intended only for suitably qualified service
technicians.
In the interest of the safety of patients and all operators:
Only service technicians trained and authorized by SFRI Sarl FRANCE may service the H18 Light V2.
Please refer to the operating instructions for any information you need on operating the H18 Light V2.
This Service Manual contains references to the corresponding chapters in the operating instructions.
(If required).

1. SPECIFICATIONS
Class
According to anti-electric shock: Grade II, Pollution 2
According to defend deleterious liquid: General Equipment (Closed Equipment)
According to disinfection method: Recommend by manufactory
According to safety degree: Do not use the equipment under the environment of mixed with
flammable air or the air is mixed with O2 and NO
According to working state: Continuous running Equipment
Règlementation
DIV 98/79
1.1. Front Face View
Screen
Right Panel
Instrument
Status LED
Sample Probe
Window
Start Key
Thermal Printer
Door
Picture 1-1

1.2. Back Panel View
11
1
12
2
13
3 14
4 15
16
5
17
6
18
19
8
9
10
Picture 1-2
# Description
1 Parallel Printer Port
2 PS2 Mouse Port
3 PS2 Keyboard Port
4 Equipotential Connector
5 Power Switch
6 Lyse Inlet Tube Connector
7 Waste Full Sensor Connector
8 Waste Outlet
9 Cleaner Inlet Tube Connector
10 Diluent Inlet Tube Connector
11 Cooling Fan (Expulses air)
12 USB Port 1
13 USB Port 2
Table 1-1

# Description
14 VGA Port
15 RS232 Port, COM 1
16 RS232 Port COM 2
17 Cooling Fan (Aspirates air, with air filter)
18 Power Socket with Fuses Housing
19 Switching Power Supply Cooling Fan
Table 1-1
1.3. System Specifications

1.3.1. PARAMETERS
20 parameters and 3 histograms
Measured parameters
ITEMS UNITS
9
WBC 10 / L
RBC 1012 / L
MCV fL
HGB G/L
PLT 109 / L
MPV fL
Table 1-2
Parameters given by histogram
ITEMS UNITS
LYM% %
MON% %
GRAN% %
RDW-SD fL
RDW-CV %
PDW %
P-LCR %
Table 1-3
Calculated parameters
ITEMS UNITS
LYM# 109 / L
MON# 109 / L
GRAN# 109 / L
HCT %
MCH pg
MCHC g/L
PCT %
Table 1-4

1.3.2. VOLUMES
Sample Volume Reagent Volume per test

Venous & Capillary modes : 14 μl Diluent : 20.2 mL
Prediluted mode: 20.0μL Rinse : 5.3 mL
Lyse : 0.50 mL
1.3.3. DILUTION
Dilution Ratio
Venous and Capillary mode: Prediluted Mode:
WBC/HGB 1:300 WBC/HGB 1:355
RBC/PLT 1:44600 RBC/PLT 1:44500
Display Language
8” LCD screen English
High resolution: 640x480 mm
Color display
1.3.4. INPUT/OUTPUT
Electrical input x 1 VGA x 1

Print socket x 1 RS-232 x 2
USB x 2 PS/2 x2
1.3.5. PRINTOUT
On board fast thermal printer, 57.5 mm wide paper, recording width 48 mm

Optional external printer, models recommended: Epson LX300+ and Panasonic KX-P1121
1.3.6. LINEARITY AND PRECISION
PARAMETERS LINEARITY RANGE PARAMETERS LINEARITY RANGE

9
WBC 0.0 -99.9x10 /L GRAN# 0 -99.9x109/L
RBC 0.00 -9.99x1012/L HCT 0.0 -100.0%
HGB 00.0 -300g/L MCH 0.0 -999.9pg
9
PLT 0 -3000x10 /L MCHC 0.0 -999.9g/L
MCV 0 -250fL RDW-SD 0.0 -99.9 fL
LYM% 0 -100% RDW-CV 0.0 -99.9%
MON% 0 -100% PDW 0.0 -30.0%
GRAN% 0 -100% MPV 0.0 -30.0fL
9
LYM# 0 -99.9x10 /L PCT 0.0 -9.99%
MON# 0 -99.9x109/L P-LCR 0.0-99.9%
Table 1-4

2. INSTALLATION PROCEDURE
2.1. Pre-Installation on Site Inspection

2.1.1. SPACE REQUIREMENTS
H18 Light measures 330(W) x 430(H) x 380(D) mm. There should be adequate space for the
instrument as well as the mouse, keyboard, external printer (optional), external barcode
reader (optional). Make sure that there is at least 15 cm of clearance around the top, sides and
back of the instrument. Ensure that there is adequate space for reagents either under the
counter or at counter level. Instrument weight is 17,5 kg. Make sure you will enough space to
perform annual preventive maintenance or on demand repair.
2.1.2. LOCATION REQUIREMENTS
The H18 Light should be located in a controlled environment free from dust, mechanical
vibrations, loud noises and electrical / magnetic interferences. Avoid proximity to brush type
motors, flickering fluorescent lights and electrical contacts that regularly open and close. Avoid
placing the instrument in direct sunlight or in front of a source of heat or drafts. The instrument
should be kept in a temperature controlled environment between 18°C to 35°C (65°F to 95°F)
with humidity between 10% to 90% non-condensing.
2.1.3. POWER REQUIREMENTS
H18 Light requires a properly grounded outlet supplying 100 – 264 Va.c., 47 to 63 Hz. Power
consumption is 150 VA max.
We recommend the use of a 300 VA UPS.
Humidity should be less than 70%
Atmospheric pressure has to be between 86.0 and 106 kPa.
2.2. Pre-Installation Instrument Inspection

A pre-installation inspection of the instrument upon arrival at the distributor’s facilities is strongly
recommended. Even though the H18 Light is carefully packed, damage can occur during shipment.
Perform the installation procedures contained within this chapter to verify the operational integrity of
the H18 Light.
NOTE: theft or damage to the H18 Light and related systems is not responsibility of SFRI France. It is
responsibility of the consignee to fill all claims with the freight carrier and to make sure that all
shipments are properly insured.
2.3. Unpacking / Installing the H18 Light

IMPORTANT: Read this section completely before powering on the unit. Any attempt to power on
without following this procedure will void warranty.
DO NOT SWITCH ON THE UNIT BEFORE READING COMPLETELY THIS SECTION

i. Examine the shipping containers for any damage. If there is any obvious damage,
photograph the damage.
ii. Carefully remove the tape from the H18 Light shipping container.
iii. Open the container and examine the instrument for any signs of damage. If any
damage is present, photograph the damage and report it immediately to the carrier.
iv. The instrument must not be installed in direct sunlight or near heating and air
conditioning vents.
v. Do not install the H18 in carpeted areas. A static discharge may result and damage
sensitive components.
vi. Remove the H18 from the shipping container and place the instrument in its final
location. Save the shipping container and package inserts.
vii. With the Allen wrench 2.5 remove the two screws of the right side panel (cf Picturexx),
and pull it out by sliding it backward.
Picture 2-1
viii. Take out the tie wrap blocking the sample arm like shown in the photograph bellow
Picture 2-2

ix. Take out the tape covering the counting chamber, making sure you are not living any
piece of tape.
Picture 2-3
Picture 2-4
x. Remove the fluidic ports protectors in the back side of the unit
xi. Connect the reagents supply tubes in the inlet fluidic ports (rear panel).
xii. Place the reagents near the instrument.
DO NOT INCREASE THE LENGTH OF ANY OF THE REAGENT PICKUP TUBE

ASSEMBLIES.

xiii. Insert the reagent pickup tubes in their respective reagent. The reagent pickup tubes
have color coded luer caps that correspond to the luer connections on the rear panel
of the instrument. Remove the shipping plugs from the luer connections. Make sure
that the reagent pickup tubes are placed in the proper reagent. (Green = Diluton, Blue
= Diluterge, and Red = Lysoglobine).
xiv. Connect the waste tube assembly to the back of the H18 Light.
DO NOT INCREASE THE LENGTH OF THE WASTE TUBE ASSEMBLY.

xv. Inflate the waste container and attach the waste tube assembly to the container.
Place the waste container on the floor. Waste may also be directed down a sink drain.
DISPOSE OF WASTE IN ACCORDANCE WITH GOVERNMENT REGULATIONS.

xvi. Save shipping containers in a cool, dry place.

2.4. Performances verification
2.4.1. BACKGROUND
We call background a measurement done without aspirating any blood, but air. In order to
avoid registering the results of background tests in the DATALOG, you may enter 999999 code
in patient Id.
Press the Start Key without presenting any tube to the sample probe and let the instrument
perform a complete counting cycle. The results given by the unit should comply with the
following table:
Item Range
WBC 0.0 – 0.2 x 109/L
RBC 0.00 – 0.02 x 106/µL
PLT 0 – 6 x 103/µL
HCT 0.0 – 0.5 %
Table 2-1
Should the Background results be out of tolerances, please refer to Trouble shooting section.
2.4.2. STABILITY : CV
Once the system is able to perform at least five backgrounds in range you can go through the
stability verification.
Run 15 replicates of a normal level SFRI blood control in factory mode (Refer to Software
Chapter) and make the instrument calculate for you the CV.
If the CV values are in range (see table 2-2 below), then see if they are in target, ie, if the results
comply with the control target. If they match, the system is ready to be used. If they are not
you have to perform a calibration.
PARAMETERS PRECISION CV%

WBC ≤2.5%
RBC ≤2.0%
HGB ≤2.0%
MCV ≤1.0%
PLT ≤5.0%
Table 2-2
SFRI Blood Control:

Name: BLOODTROL, Item Code: R021005, Expiration (close): 105 days after production,
Expiration (open): 2 weeks after opening the vial.
NOTE: NEVER CALIBRATE A SYSTEM WHICH IS NOT STABLE

2.4.3. CALIBRATION
IMPORTANT NOTE:
H18 Light System has two sets of calibration factors.
One set is the factory one, and can be seen and adjusted in “Factory” mode of the software.
The second set is the user one, and can be adjusted manually or automatically by the user, in
“Normal” software mode.
If your system is stable and the values are not in range, you may need to calibrate the unit.
Use only SFRI Calibration Blood, and follow the calibration procedure as described in Operator
Manual version H18 LIGHT_Operator Manual_ENG_V12.00 or above.

3. THEORY OF OPERATION
3.1. Counting Blood Cells

3.1.1. COULTER PRINCIPAL
The Coulter principle states that particles pulled through an orifice, concurrent with an electric
current, produce a change in impedance that is proportional to the volume of the particle
traversing the orifice. This pulse in impedance originates from the displacement of electrolyte
caused by the particle. The Coulter principle was named for its inventor, Wallace H. Coulter
when he was working for the Department of Naval Research to standardize the size of solid
particles in the paint used on US battleships in order to improve its adherence to the hull. The
principle has found commercial success in the medical industry, in particular in hematology,
where it can be applied to count and size the various cells that make up whole blood.
Cells, being non-conducting particles, alter the effective cross-section of the conductive micro
channel. If these particles are non-conducting, the electrical resistance across the channel
increases, causing the electric current passing across the channel to briefly decrease. By
monitoring such pulses in electric current, the number of particles for a given volume of fluid
can be counted. The size of the electric current change is related to the size of the particle,
enabling a particle size distribution to be measured, which can be correlated to mobility,
surface charge, and concentration of the particles.
Picture 3-1

3.1.1.1. OHM LAW
The Coulter principal basically deals with the well known Ohm low:
U=RxI
Where U is the voltage, R the resistance and I the current.
A constant current generator is applied to the electrodes of two chambers separated by

an orifice, filled with electrolyte (diluent). This current generates a base line voltage
whose value changes while blood cells are crossing the orifice.
As a result, the measured voltage is a direct function of the size of the cells crossing the
apertures.
U = f(ps)
Where U is the voltage and ps the particule size.
3.1.2. TREATING THE SIGNAL
3.1.2.1. THREASHOLDS
Each cell crossing the apertures will create a signal, a voltage pulse, which amplitude will
be compared to triggers, allowing their classification on WBC, RBC and PLT. These
thresholds are software values that are inside the executable program. Neither the user
nor the service people are able and allowed to modify these values.
3.1.2.2. HISTOGRAMS
Each pulse will be analyzed and stored to a specific memory area, depending on its
characteristics. Once the counting time is over (after 12 seconds), the memorized data is
processed and the histograms are performed. It is then possible to get any histogram
derived data.
3.2. HGB reading

Adding lyse in the blood, the red blood cell will be hemolyzed and hemoglobin released. Hemoglobin
and lyse form a new mixture, which can absorb the wavelength of 540nm. Absorbency is then
measured. Through the comparison of the absorbency between the pure diluent and the sample, the
concentration of the sample hemoglobin is calculated.
Picture 3-2

4. FLUIDICS SYSTEM
4.1. Fluidics phases

4.1.1. STAND BY
After initialization and between each sample counting, the unit is in standby mode.
All the reagents are primed and the chambers are filled with diluent.
Vacuum / Pressure chamber is at atmospheric pressure.
The constant current generator is off.
Burn voltage is off.
Dilution mechanism and vacuum /pressure mechanism are at home position while sample
probe arm is positioned ready to pipette a new sample blood.
All valves are off (ie, not energized). Two ways valves are closed, and three ways valves are in
NO (Normally Opened) position.
HGB lamp is on.
Motors power is off.
4.1.2. DILUTION
The blood tube is presented to the sample probe, start switch is pushed, 14 µL of sample is
aspirated. A first dilution is done in the WBC chamber. Dilution ratio is 1/300 in venous and
capillary mode. A little amount of volume is then aspirated to prepare the second dilution in
the RBC chamber. Dilution ratio is 1/44600. Lyse is dispensed to WBC chamber: 500 µL. HGB is
read by the spectrophotometer.
4.1.3. COUNTING
As soon as the dilutions are ready in both chambers, the vacuum/pressure pump generates an
accurate and stable counting vacuum. The cells are then hydro-focused in a fluidic current in
order to be counted separately during 12 seconds.
Simultaneously, RBC, PLT, and WBC are counted. RBCs and PLTs are counted in the RBC
chamber and the WBCs are counted in the WBC chamber.
The software is controlling the consistency of cells circulation in order to detect any fluidics
problem.
CAUTION: Vacuum value is not controlled by the system. It is important to run

every morning a quality control blood to make sure this function is performed
properly.
In principal if the preventive maintenance is done according to SFRI recommendation, the
vacuum should be operating properly between at least two preventive maintenance visits.
Should you suspect any counting vacuum problem please use an external manometer to make
sure this function is complying with the specs.
4.1.4. CLEANING

Once the counting is finished, the chambers are emptied (to external waste), the counting line
is cleaned with Diluterge primed by the vacuum pump. The chambers are cleaned internally
with diluent and finally filled again with diluent, in order to get back to standby mode. In the
mean time the probe has been cleaned internally as well with diluent.
You can find an accurate explanation of the fluidics phases in

Appendix 4

4.2. Fluidic Algorythm
Picture 4-1

4.3. Fluidic schematic
Picture 4-2

4.4. Fluidics components
4.4.1. FLUIDICS PARTS LIST
Reference Item Designation Item Code Length

G1 TYGON® S-50-HL Class VI S020059 850 mm
G24 TEFLON ----------- 120 mm
G34 TYGON® S-50-HL Class VI S020058 -----------
G47 TEFLON ----------- 103 mm
Table 4-8

4.4.2. RBC CHAMBER : ITEM CODE S020010
The second dilution is performed in this chamber used to count the RBCs and the PLTs. Its ratio
is 1/44600.
The aperture of this chamber has an 80 µm orifice and the aperture voltage range is between
13 Vdc and 23 Vdc. The ruby separates two areas of the chamber; in each of them an electrode
is located, driving either the counting controlled constant current or the “high AC voltage”, in
charge of the burning function coming from the CCU board.
The electrodes of the chamber are connected to the CCU Board at J28 / J30
Picture 4-3 Picture 4-4
# Item Code Item Designation

1 NA Counting Chamber with HGB sensor Assy
2 NA Aperture to Chamber Fixing Block (x1)
3 NA Silicon Spacers (x2)
4 NA Aperture (Rubis) / 100 µm, V shaped (x1)
5 NA Screw (x2)
Table 4-1
4.4.3. WBC CHAMBER
ITEM CODE : S020009

FUNCTIONS: white blood cells counting and HGB reading
The first dilution is performed in this chamber. Its ratio is 1/300.

The aperture has a 100 µm orifice and its aperture voltage range is between 9 Vdc and 19
Vdc.
The HGB Assy is mounted on this chamber: LED 540 nm light source, and photo sensor. Both
are connected to CCU board to connector J21.

The electrodes of this chamber are connected to CCU board at J25 / J29
Picture 4-5 Picture 4-6

1 NA Counting Chamber with HGB sensor Assy
2 NA Aperture to Chamber Fixing Block (x1)
3 NA Silicon Spacers (x2)
4 NA Aperture (Rubis) / 100 µm, V shaped (x1)
5 NA Screw (x2)
Table 4-2
4.4.4. DILUTION BLOCK
ITEM CODE: S020024
FUNCTIONS:
The function of this assembly is to prime and aspirate / dispense reagents, sample blood
(sample, control, and calibrator). It is composed of a single Teflon block, in which 3 chambers
and their corresponding pistons are in charge to deliver accurate volumes of blood, diluent
and lyse.
The three pistons are mechanically linked and thus are moving at the same time. The valves
activation program allows proper volumes delivery as required.
IMPORTANT: the sample piston has a specific design, making possible to control the other two
pistons without any consequence on the sample one. Two mechanical stops, located at specific
position allow this to be possible. Refer to picture 4-8 below (mark 2).
NEVER TOUCH THESE TWO STOP NUTS, THEIR POSITION IS FACTORY

ADJUSTED AND NEVER HAVE TO BE CHANGED.
CAUTION: the part 4 shown in picture 4-8, is a precision Teflon guide. Please handle with care
while performing maintenance on the Dilution Block.

Picture 4-7

1 Not available Dilution Block
2 Not available Diluent Piston
3 Not available Lyse Piston
4 S020032 Diluent Piston Seal
5 S020030 Lyse Piston Seal
6 Not available Pistons Maintaining Bracket
10 Not available Sample Piston Assy
Table 4-3
SAMPLE PISTON ASSY DETAIL
Picture 4-8

1 Not available Sample Piston
2 Not available Sample Piston Stops
3 Not available Teflon Stopper Screw
4 Not available Teflon Sample Piston Slide Guide
5 Not available Teflon Sample Piston Spacer
6 S020060 Sample Piston O-Ring
Table 4-4
4.4.5. VACUUM BLOCK
ITEM CODE : S020022
FUNCTIONS :
ü Counting vacuum: provide: accurate and stable vacuum for the counting phase
ü Mixing: Provide positive pressure to send air bubbles to the chambers, mixing function
ü Priming the cleaner during cleaning phase
ü Waste the vacuum pump contain
Picture 4-9

1 Not available Vacuum Housing Piston
2 S020033 Vacuum Piston Seal
3 Not available Vacuum Piston
4 S020065 Vacuum Rod
5 Not available Home Flag
Table 4-5

4.4.6. SAMPLE PROBE WASHER
ITEM CODE: S020026
FUNCTIONS:
Wash the external part of the sample probe after blood aspiration
Picture 4-10

1 Not available Sample Probe Washer Block
2 Not available Sample Probe Washer Cover
3 S020034 Sample Probe Washer O-ring
Table 4-6
4.4.7. TWO WAYS AND THREE WAYS VALVES
ITEM CODE: S020007 (Two way valve) / ITEM CODE: S020008 (Three way valve)
FUNCTION TABLE
Valve Number Valve Type Valve Function

V2 Two ways Sample Probe Washer Aspiration
V3 Two ways Atmospheric pressure port
V4 Two ways Cleaner dispensing valve
V5 Two ways Counting valve
V6 Two ways Waste valve
V9 Three ways Lyse dispensing valve
V10 Three ways Diluent path selection (dilution or external probe washing)
V11 Three ways Diluent path selection (Probe washer or ruby – external)
V12 Three ways Diluent Priming Valve
Table 4-7

5. ELECTRONICS
5.1. Switching Power Supply

5.1.1. ITEM CODE: S020056-V2
5.1.2. FUNCTIONS
- Provide +12Vdc and +5Vdc to CPU board (via CCU, and jumper JP1) and CCU board
- +5Vdc / 4A is intended to power the CPU board
- +12 Vdc is intended to power the CCU board
- Provide +5Vdc and +12Vdc to
-
5.1.3. NOTE
- An internal trimmer allows the adjustment of these voltages, paying attention that the
CPU voltage mustn’t be lower than 4,5 Vdc
- Two fuses of 3,15A (delayed)
5.2. Central Command Unit, CCU board

5.2.1. ITEM CODE: S020003-02
5.2.2. FUNCTIONS
5.2.2.1. ACTUATORS
o Motors driver
§ Motor A (1): Sample probe vertical movement
§ Motor B (2): Sample probe horizontal movement
§ Motor C (3): Dilution block movement
§ Motor D (4): Vacuum / Pressure pump
o 2 ways valves
o 3 ways valves
o Constant current generator (coulter principal cells counting)
o High AC voltage for “burn” function
5.2.2.2. SENSORS
o Start switch (mechanical)

o Waste full (resistive)
o Reagents presence (optical): diluents, cleaner and lyse
o Diluent Temperature (resistive)
o HGB spectrometer
o Cells counts (resistive - WBC, RBC, PLT)
5.2.2.3. TECHNICAL DESCRIPTION
The board has been developped around a CPLD (Complex Programmable Logic Device)
powered by a voltage of 3,3 Vdc, supplied by the positive voltage regulator LT1117.

ü XC95288XL: high performance CPLD, low-voltage applications in leading-edge
communications and computing systems. It is comprised of 16 54V18 Function Blocks,
providing 6,400 usable gates with propagation delays of 6 ns.
ü HCT373M: The SL74HCT373 is used as a level converter for interfacing TTL or NMOS
outputs to High-Speed CMOS inputs.
ü HC14M : HEX SCHMITT INVERTER
ü T89C2051 (V1.0 – 6008) : microcontrôleurs 8 bits
ü HCT244A : Octal 3−State No inverting Buffer/Line Driver/Line Receiver with
LSTTL−Compa ble Inputs
ü IDT7203: The IDT7203 (CMOS ASYNCHRONOUS FIFO) is a dual-port memory buffer
with internal pointers that load and empty data on a first-in/first-out basis. The device
uses Full and Empty flags to prevent data overflow and underflow and expansion logic
to allow for unlimited expansion capability in both word size and depth.
ü ADC0820: 8-Bit High Speed µP Compatible A/D Converter with Track/Hold Function
ü HCT245 is an advanced high-speed CMOS OCTAL BUS TRANSCEIVER (3-STATE). This IC
is intended for two-way asynchronous communication between data buses, and the
direction of data transmission is determined by DIR input. The enable input G can be
used to disable the device so that the buses are effectively isolated. All inputs are
equipped with protection circuits against static discharge and transient excess voltage.
All floating bus terminals during High Z State must be held HIGH or LOW.
ü AD586 (High Precision 5 V Reference) represents a major advance in the state-of-the-
art in monolithic voltage references. Using a proprietary ion-implanted buried Zener
diode and laser wafer trimming of high stability thin-film resistors, the AD586 provides
outstanding performance at low cost.
ü 8L05A 3-Terminal Positive Voltage Regulator (5Vdc). These regulators employ internal
current-limiting and thermal-shutdown, making them essentially indestructible. They
can deliver up to 100mA output current.
ü MAX4051 Low-Voltage, CMOS AnalogMultiplexers/Switches
ü A3977SED-T Microstepping DMOS Driver with Translator. The A3977xED and
A3977xLP are complete microstepping motor drivers with built-in translator. They are
designed to operate bipolar stepper motors, with output drive capability of 35 V and
±2.5 A. The translator is the key to the easy implementation of the A3977. By simply
inputting one pulse on the STEP input the motor will take one step (full, half, quarter,
or eighth depending on two logic inputs). There are no phase-sequence tables, high-
frequency control lines, or complex interfaces to program. The A3977 interface is an
ideal fit for applications where a complex μP is unavailable or over-burdened. Internal
synchronous-rectification control circuitry is provided to improve power dissipation
during PWM operation.
ü ULN2003 SEVEN DARLINGTON ARRAYS. ULN 2003 is a high voltage, high current
Darlington arrays each containing seven open collector Darlington pairs with common
emitters. Each channel rated at 500mAand can withstand peak currents of
600mA.Suppressiondiodesare included for inductive load driving and the inputs are
pinned opposite the outputs to simplify board layout. These versatile devices are
useful for driving a wide range of loads including solenoids, relays DC motors, LED
displays filament lamps, thermal printheads and high power buffers.
ü LM339 SINGLE SUPPLY COMPARATORS: these are used in the reagent detection circuit.
ü LM134 3-Terminal Adjustable Current Sources
ü OP37G LOW-NOISE HIGH-SPEED PRECISION OPERATIONAL AMPLIFIERS : Cells counting
signal amplification. The OP27 and OP37 operational amplifiers combine outstanding
noise performance with excellent precision and high-speed specifications. The
outstanding characteristics of the OP27 and make this device excellent choice for low-
noise amplifier applications requiring precision performance and reliability

ü TL072BC LOW NOISE J-FET DUAL OPERATIONAL AMPLIFIER. The TL072B is high speed
J–FET input dual operational amplifier incorporating well matched, high voltage J–FET
and bipolar transistors in a monolithic integrated circuit. The devices feature high slew
rates, low input bias and offset current, and low offset voltage temperature
coefficient.

5.2.3. FUNCTIONAL SCHEM
Picture 5-1

5.2.4. COMPONENTS LAYOUT
5.2.4.1. FRONT VIEW
Picture 5-2

5.2.4.2. REAR VIEW
Picture 5-3

5.2.5. CONNECTORS’ DESCRIPTION TABLE
Connector # Description
J1 Not Used
J2 Power Supply
J3 Home Sensor Motor A (Probe Vertical Movement)
J4 Command Power Motor A
J5 Home Sensor Motor B (Probe Horizontal Movement)
J6 Command Power Motor B
J7 PC 104 Connector
J8 Home Sensor Motor C (Dilution Block)
J9 Command Power Motor C
J10 PC104 Connector
J11 Home Sensor Motor D (Vacuum Pump)
J12 Command Power Motor D
J13 Solenoid Valves Connector (V1 to V8)
J14 Solenoid Valves Connector (V9 to V14)
J15 Lyse presence detector (optical sensor)
J16 Distribution Board
J17 Cleaner presence detector (optical sensor)
J18 Diluent presence detector (optical sensor)
J19 Waste Full Sensor (Resistive)
J20 Start Switch
J21 HGB Sensor Assy
J23 Diluent Temperature
J24 On Board Thermal Printer
J25 WBC Chamber
J26 Front Panel LED Status Indicator
J27 Burn Trasnformer
J28 RBC Chamber
Table 5-1

5.2.6. TEST POINTS TABLE
TP # TP Description Comment
TP1 Ground
TP2 Not Used
TP3 2,5 Vdc
TP4 Reagent Temperature Sensor
TP5 Cleaner Presence Sensor Typical value 4 Vdc with liquid, bellow 1,5 Vdc without
TP6 Not Used Not Used
TP7 Diluent Presence Sensor Typical value 4 Vdc with liquid, bellow 1,5 Vdc without
TP8 Lyse Presence Sensor Typical value 4 Vdc with liquid, bellow 1,5 Vdc without
TP9 RBC Counting Pulses
TP10 Not Used Not Used
TP11 PLT Counting Pulses
TP12 A/D Convertor Clock
TP13 WBC Counting Pulses
TP14 HGB OD Signal
Table 5-2

5.3. Central Processing Unit: CPU board
5.3.1. ITEM CODE: S020001-02
5.3.2. FUNCTIONS:
This is the HID of the instrument. This industrial computer provides all basic input and output
system for the user, and communication with machine level software, ie, communication with
the CCU firmware through a PC104 bus.
A compact flash memory, or a Disc On Module, connected to the CPU, contains all BIOS, OS
and application software, allowing the unit to operate. The picture below shows all the
connectors detail.
Picture 5-4

5.4. Liquid Sensor Adj. /Connection Distribution
Board
5.4.1. ITEM CODE: S020063-02
5.4.2. FUNCTIONS:
This board interfaces the connections between the CCU board and the actuators and sensors.
It also has a dedicated electronics to adjust the optical reagent detection sensitivity, as shown
in detail E.
Detail D shows the valves connection interface, detail C the sensors, and detail B the actuators.
Picture 5-6

5.5. LCD Adaptor Board
5.5.1. ITEM CODE: S020067
5.5.2. FUNCTION:
The main function of LCD Adaptor Board is to connect the screen to the CPU with cable. It
interfaces TFT through FPC soft cable.
Picture 5-7
5.6. On Board Thermal Printer Driver Board

5.6.1. ITEM CODE: S020028 (COMPLETE ASSEMBLY, INCLUDED PRINTER HEAD)
Picture 5-8

6. MECHANICS
6.1. Sample probe arm

6.1.1. ITEM CODE: S020062-02
Picture 6-1
6.1.2. FUNCTION
This mechanical assembly allows the system to position the sample probe at operator disposal,
to aspirate the sample, calibrator or control blood and then to the counting chambers, in order
to perform the dilutions.
While performing maintenance, the mechanical system will cover the same function but
Hemaclair or Diluclair will replace the blood.
Two movements are carried out to perform these positioning: vertical and horizontal.
Thus, two stepper motors are involved, Motor A (vertical movement), and Motor B (horizontal
movement). Each movement has a home position, controlled by an optical sensor.

6.2. Dilution Machine
6.2.1. ITEM CODE: S020023
Picture 6-2

6.3. Vacuum Pump Machine
6.3.1. ITEM CODE: S020064
Picture -6-3

7. SERVICE SOFTWARE
7.1. Mechanics
This Service Program allows the service engineer to troubleshoot, by checking actuators and sensors.
Basically, this program sends single commands to the CCU.
From the main screen select Service, Mechanic.
Picture 7-1

Enter password 6100 when prompted. You’ll reach the following screen.
Picture 7-2
We do have four main parts in this screen:
7.1.1. ELECTRONIC VALVE:

Here we can activate each valve for a couple of seconds by clicking on its “T” command.
Doing so, you have to hear the commutation sound, which is louder for the two way valves.
NOTE: Valves 1, 7 and 8 are not used. The “T” command is grey, and a click on it has no effect.
Valve 15 actually is a relay command
7.1.2. MOTOR
A click on “T” command of each motor will generate a movement to get the mechanical part
to home position. If the mechanism is already at home position the firmware will move the
flag out and in again. If the mechanism is out of home position the firmware will move the flag
toward home position.
If the system doesn’t detect any error on the movement the software will send an “Ok” status,
otherwise “Failed” will be prompted.
ü Motor A: Vertical Movement of the Sample Probe Arm
ü Motor B: Horizontal Movement of the Sample Probe Arm
ü Motor C: Dilution Block Movement
ü Motor D: Vacuum Pump Movement

7.1.3. CIRCUIT
This part of the Mechanic software is dedicated to test the CCU board
7.1.3.1. ANALOGICAL / DIGITAL CONVERTOR

WBC AD / RBC AD / PLT AD: test of the Analogical to Digital converters for each channel.
There are three converters, one by cell type. The system sends a value to convert and the
software tests the feedback. The system returns “OK” if the returned value is the good one
and “Failed” if not.
7.1.3.2. INTERRUPT REQUEST / MICRO CONTROLLER

This function allows to test the interruptions control between main micro-controller of the
CCU and the dedicated 3 micro-controllers in charge of the cell pulses acquisition (WBC,
RBC, PLT pulses).
7.1.3.3. 5V POWER
This is an integrated voltmeter returning the actual +5 Vdc value.
7.1.3.4. HGB
This function returns the actual HGB sensor reading. Please refer to Adjustments and
Controls chapter for more details.
7.1.3.5. VACUUM
This function creates a specific vacuum value (counting vacuum). Activating this command
will make the vacuum piston move up to predefined specific position.
7.1.3.6. DC POWER
This is the “Enable” signal sent by the software to the CCU board.
7.1.3.7. CC POWER
This function allows you to test the Constant Current generator. A click on its “T” command
will activate relays K4 and K5, as a result the Constant Current will be sent to the electrodes
of both counting chambers inorder to generate the base line voltage.
7.1.3.8. BURN
This function sends 110 Vac directly to the electrodes of both counting chambers in order
to clean the apertures and prevent clogs that could appear due to orifice diameter
reduction while proteins get agglutinated. Relay K2 is activated to send this “high” AC
voltage to the chambers.
7.1.3.9. R. TEMP
This function returns the actual diluents’ temperature, measured by the sensor integrated
in the sample line.
7.1.3.10. SPEAKER
Internal speaker test. You should hear a little “pock” sound.
7.1.3.11. WBC HOLE / RBC HOLE

These two functions are working together while clicking on “T1”.
The system will generate a vacuum, open the counting valve V5, and send the constant
current to the electrodes in order to generate the base line voltage. The current aperture
voltages will be displayed after a couple of seconds. The values have to be as follow:

ü WBC Hole between 9 and 19 Vdc
ü RBC Hole between 11 and 13 Vdc
Then the system goes back to stand by, ie, stops the constant current and the diluent
circulation. The measured value remains in the screen.
An action on the T2 command will refresh the actual baseline voltage value that has to drop
below 3 Vdc. Tipical value is around 1,5 Vdc.
7.1.3.12. INTERFACE
You can test the three peripheral dedicate to receive results.
ü On board thermal printer (Recorder)
ü External Printer
ü RS232 Serial Port
7.2. Special
7.2.1. FACTORY MODE
From main screen :

- Service -> Special -> Enter password « 6666 ».
- Press F12
- Check that the H18 is actually in Factory Mode (you should see SFRI France in the bottom left
corner of the screen, and “Factory” appears in the main title screen (Top center).
The two principal functions you access in factory mode are:

ü The factory calibration factors
ü CV calculation functionality
NOTE: In factory mode, the apertures voltages are displayed in the

left bottom area of the screen, in red. This functionality allows you
to check if the aperture voltages are stable and in range.
Pressing F12 will switch from normal to factory mode unless you enter again 6666 password
as explained above, or instrument is powered off.
IMPORTANT NOTE: never leave the unit with factory mode accessible
for the end user.
All the standard functions of the unit are the same than in user mode.
7.2.2. PARAMETERS SCREEN
You should print and save these parameters before sending the unit at customer site
Access to this screen:

- Service -> Special -> Enter password 6161
The “Parameters Adjust screen is shown here below:

Picture 7-3
The parameters adjust screen is divided in two parts.
7.2.2.1. PARA
Do not attempt to adjust these parameters, they are factory set and don’t need any
adjustment in the field, unless SFRI authorized specialist indicates you to perform changes
there.
7.2.2.2. FLUIDICS
WBC & RBC Time (s): Software fixed at 12 seconds. This value mustn’t be changed.
WBC & RBC Hole: These are the reference values that the system uses in order to return a
clog situation. Refer to adjustment chapter for procedure.
WBC & RBC threshold: Factory values that don’t have to be changed.
Temp. Low: sets the minimum room temperature allowed to the system to work.
Temp High: sets the maximum room temperature allowed to the system to work.
Motor A, B, C and D: motor steps for mechanics positioning. Factory adjusted, they don’t
have to be modified.

7.3. Network Transfert Protocol
7.3.1. INTRODUCTION
SFRI H18 Light hematology analyzer use RS-232 port as a network transmission. There are
three data lines on transmission: TxD, RxD, GND. The data transfer to the receiving device by
an one-way transmission, not response of data request for any external device. This protocol
adopts characteristic identification method and data block on transmission. All the decimal
points could not be transferred in practice.
7.3.2. TRANSFER PROTOCOL
Baud Rate 9600

Data Bits 8
Parity None
Stop Bits 1
Table 7-1
7.3.3. WIRING
Picture 7-4
7.3.4. DATA BLOCK FORMAT
Start-identifier of data block: @ MCH: ###.#

Identifier of data block: a Alarm: # (#: normal, h: high, l: low)
Sample No.: ########### MCHC: ####
Year: #### Alarm: # (#: normal, h: high, l: low)
Month: ## RDW-SD: ##.#
Date: ## Alarm: # (#: normal, h: high, l: low)
Hour: ## RDW-CV: ##.#
Minute: ## Alarm: # (#: normal, h: high, l: low)
Second: ## PLT: ###

WBC: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) MPV: ##.#
LYM%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) PDW: ##.#
MID%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) PCT: #.##
GRAN%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) P-LCR: ##.#
LYM#: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) Reserved: ########
MID#: ##.# Low temperature: #(#: normal, t: temperature)
Alarm: # (#: normal, h: high, l: low) WBC bubble: #(#: normal, b: bubble)
GRAN: ##.# WBC clog: #(#: normal, c: clog)
Alarm: # (#: normal, h: high, l: low) RBC bubble: #(#: normal, b: bubble)
RBC: ##.# RBC clog: #(#: normal, c: clog)
Alarm: # (#: normal, h: high, l: low) Reserved: ########
HGB: ### RM: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) R1: #(#: normal, r:alarm)
HCT: ##.# R2: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) R3: #(#: normal, r:alarm)
MCV: ###.# R4: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) PM: #(#: normal, r:alarm)
Reserved: ########
Lower limit of WBC first line: ###
Upper limit of WBC first line: ###
Lower limit of WBC final line: ###
Upper limit of WBC final line: ###
Lower limit of RBC: ###
Upper limit of RBC: ###
Lower limit of PLT: ###
Upper limit of PLT: ###
Reserved: ########
WBC histogram: ### (total number: 256)
RBC histogram: ### (total number: 256)
PLT histogram: ### (total number: 256)
End-identifier of data block: &
7.3.5. USAGE OF WBC, RBC, PLT HISTOGRAM DATA
The total number of the received data between (0,255) is 256. If there are 256 points on the
X-axis of the histogram, set the proportion of Y-coordinates as “a”. Divide the 256 received
data respectively by “a”, the results as the values of Y-coordinates. Draw the corresponding X-
coordinates in the received order.
7.3.6. EXAMPLE
The following is a complete data flow example.
Start-identifier of data block: @ Alarm: #

Identifier of data block: a HCT: 361
Sample No.: 050603008 Alarm: #
Year: 2005 MCV: 0806

Month: 06 Alarm: #
Date: 03 MCH: 0226
Hour: 21 Alarm: #
Minute: 43 MCHC: 0263
Second: 08 Alarm: #
WBC: 069 RDW-SD: 465
Alarm: # Alarm: #
LYM%: 269 RDW-CV: 150
Alarm: # Alarm: #
MID%: 121 PLT: 117
Alarm: # Alarm: #
GRAN%: 610 MPV: 097
Alarm: # Alarm: #
LYM#: 019 PDW: 113
Alarm: # Alarm: #
MID#: 008 PCT: 011
Alarm: # Alarm: #
GRAN: 042 P-LCR: 141
Alarm: # Alarm: #
RBC: 420 Reserved: ########
Alarm: # Low temperature: #
HGB: 095 WBC bubble: #
WBC clog: #
RBC bubble: #
RBC clog: #
Reserved: ########
RM: #
R1: #
R2: #
R3: #
R4: #
PM: #
Reserved: ########
Lower limit of WBC first line: 020
Upper limit of WBC first line: 057
Lower limit of WBC final line: 082
Upper limit of WBC final line: 255
Lower limit of RBC: 010
Upper limit of RBC: 164
Lower limit of PLT: 010
Upper limit of PLT: 214
Reserved: ########
WBC histogram: ###
RBC histogram: ###
PLT histogram: ###
End-identifier of data block: &
Here is the flow itself :
@a05060300820050603214308069#269#121#610#019#008#042#420#095#361#0860#0226#0263#
465#150#117#097#113#011#141####################################0200570822550101640
10214########00000000000000000000000000000000000000000000000000000000100200200300

500700901201201401902402903704205005806607007507607808007707607006906606005404704
303803703002602502202001901701501801701802002102302402902903203403303403403603403
503403003103002802602502302202202102002001901801801901901902002102102102202202302
302502802903303103403603804004004204504704705005205305605705906006206206005805605
505405405004804604404103903803603503403503403503302802802502402102001801801901601
601601501501501501401401201201201201101001000900900700600600700700600700700900800
700700700800700700600700700700700500500500500500500400400500500500500500500500500
400400400400400400400500500500500500500400400400400500500500500500600600600500500
500500500500500500600700600700801001001001001001001000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000100100100200200300
300400500500600700800901101301401801902302503003103503904304605005305206006306506
907007307308007507507407207106906706206105805605305004704504404103703603303202902
702402302101901801601501401401201101000900900800700600600500500500400400400400400
300300200200200200200200200200200200200200200200100100100100100100100100000100000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000300300700701401402202203003003903
904804805605606306306806807007006906906506506206205905905705705305305005004704704
604604604604604604604604504504604604604604504504304304104103903903703703603603203
203003002702702502502502502402402202202002001901901701701701701601601401401301301
201201201201201201101101001000900900900900900900800800700700700700600600600600500
500500500500500500500600600500500400400300300300300300300300300300300300300300300
400400500500600600600600600600600600600600500500400400300300300300200200200200200
200200200300300300300300300300300300300300300300300300300300300300300400400500500
600600600600600600600600600600600600600600600600600600600600700700700700800800900
900900900900900900900900900900900900900900900900904804804804805605600000000000000
000000000000000000000000000000000000000000800800000004304300000002102100000000300
300000000600600000021321300700700000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
024024021621603203208908900000001501500000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
00000000000000000&
7.3.7. HARDWARE TO TEST COMMUNICATION
Use Hyper Terminal or HTerm and a female / female DB9 crossed RS232 cable, using only RxD, TxD
and GND.
The data reception with HTerm communication software is illustrated bellow.

Picture 7-4
The related data of the transfer is shown in the following result printout
Picture 7-5

8. ADJUSTMENTS AND CONTROLS
After each Preventive Maintenance, or as required during troubleshooting, error or warning messages,
some controls and / or adjustments may be performed. You will read bellow the detailed explanation
/ procedure for each adjustment and control.
8.1. Reagent Alarm Test

Select Settings — Setting — Config, as show in picture 8-1, in the pop-up menu (Picture 8-2), set Alert
in the Sound to On, select Ok to return.
Picture 8-1

In this screen you can allow automatic printing, set the Sleep time and set the clean period.
You can also decide to print or not the limits.
Picture 8-2
At this time, it should issue a crisp Di Di sound, Waste Full alarm will be displayed in the right bottom
area of the screen.
When the system runs out of reagent, Diluent Empty, Lyse Empty and Cleaner Empty alarm
information will be displayed in the error message area of the screen.
Unplug the waste liquid short circuit connector on the back board of the H18 Light, power off and on.
The instrument should be able to start, without any warning message.
8.2. Cooling Fan Test

In the back panel of the unit two fans are in charge to keep proper internal working temperature.
One fan is aspirating air from outside (this fan has a dust filter and it is located at the bottom), the
other is extracting warm air from the inside of the unit, it is located on the top.
Make sure those fans are operating smoothly, without noise, and that the air filter is clean.
8.3. Valves Commutation Test

Select Service, Mechanic, enter password 6100.
The Electronic Valve area allows you to commutate each valve separately in order to ensure it is
operating properly.
Clicking on T button makes the corresponding valve commutate, remaining open for a couple of
seconds. You must hear the mechanical sound of commutation, taking in account that two way valves
have a louder sound than the 3 way valves.

8.4. MICROCONTROLER TEST
8.4.1. A/D CONVERSION
The analogical to digital converter is automatically checked by activating the T command of

each A/D converter (WBC, RBC and PLT). The software sends a command with data to convert
and collects the expected result. The status screen will display OK or FAILED.
8.4.2. INT
The Interruption Controller is responsible for the cells signals acquisition. To check the
interruptions request click T command of each channel, each microcontroller. The status
screen will return OK or FAILED.
8.5. Power Supply: 5Vdc

Click on T command of the 5V Power. The status screen have to display 5 Vdc +/- 0,5. An adjustment
can eventually be done on the switching power supply itself. A trimmer adjusts both together the +12
Vdc and the +5 Vdc.
8.6. HGB Blank Voltage

Click T command of the HGB, actual value is displayed. The target is 4,31 Vdc +/- 0,2 Vdc. If the value
has to be adjusted, using a special trimmer screwdriver, adjust VR2 trimmer on CCU board (figure 8-
3). To refresh the displayed current value while adjusting the trimmer, you have to click on the T button
simultaneously.
NOTE: turning VR2 clockwise decreases HGB value / counterclockwise increases HGB value.
IMPORTANT: you have to make sure before adjusting that the chamber is clean and filled with diluents,
properly primed.
VR2 Trimmer : 4,31 Vdc

±0,2
VR1 Trimmer : 0.0 Vdc

±0,2
Picture 8-3

IMPORTANT: two error messages are related to the HGB, depending on the blank voltage value read
during the test.
HGB BUBBLES: if the blank voltage is lower than 3 Vdc

HGB LAMP FAILURE: blank voltage higher than 4,8 Vdc

8.7. Accurate Counting Vacuum Check
Measuring the vacuum
i. Open the right panel of the unit by unscrewing the two allen screws (2.5 mm)
You can access to the fluidics panel
ii. Disconnect the tubing as shown in photograph #1:
Picture 8-3
iii. In the same fitting of the vacuum pump, connect the vacuum meter tubing, as shown I
photograph #2
Picture 8-4
Nota : make sure you gauge is at scale (0 at atmospheric pressure).
iv. Enter into the “Mechanic” function of the software
v. Click on “Vacuum” button, piston pump will go up…

Picture 8-5
You have to read negative pressure of 236 mbars.
vi. Click again on T button of Vacuum function. The piston will go down to home position.
vii. Disconnect the manometer tubing, and put in place the original tubing.
8.8. Burning Function Check

When click T button of Burning function, you can hear a sound directly from the chamber (be careful,
the chamber should be fill of diluents).
Using a voltmeter with chambers full of diluent:
ü Open the right panel of the unit by unscrewing the two allen screws (2.5 mm), you can access
to the fluidics panel
ü Set voltmeter to Vac
ü Introduce the red probe of the voltmeter inside the WBC/RBC chamber and apply the black
probe to ground, as shown on the photograph below:
ü Click on T button of Burning function
ü You should read a value around 90 Vac (the relay remains switched a very short time, so the
reading of the target value is furtive).
8.9. Diluent Temperature Check

Click on T command of R. Temp function. The system returns on status field the diluents temperature
in the piece of tube where the sensor is located.
This value indicates the diluent chamber temperature +/- 5°C.
Use an external calibrated thermometer in order to check the value given by the H18 Light.
NOTE: H18 Light is designed to work between 18°C and 35°C.

Make sure the room temperature where the unit is located complies with this requirement.
8.10. Speaker Check

Click on T command of Speaker allows the system to issue a sound from the buzzer of the CPU board.

8.11. Apertures Voltages Check
Clicking T1 command will initialize the fluidics to check the apertures voltages (Vacuum will be
generated and counting valve opened). CCU board will send to the electrodes the constant current,
and the related voltage will be read by the system.
The voltages values have to be in the following ranges:
WBC hole voltage: 9 to 19 Vdc

RBC hole voltage: 11 to 25 Vdc
Clicking T2 command will switch off the constant current to the electrodes. At that moment the
residual voltage displayed have to be less than 3 Vdc for both chambers.
8.12. Motors Check

This function will bring the motors A, B, C or D to their home position. Should any of them be at home
position already, it will be driven out and in again.
The intent of this function is to check the motion, smooth, without noise, and the home sensor
positioning.
8.13. Diluent Dispensing Check

Click “Settings”, “Sample modes”, “Prediluted” to enter into the prediluted measuring mode, and then
click “Service”---“Diluent”, put a 10ml graduated cylinder under the sampling needle, press the start
button, continuous add diluent 5 times, the total amount should be 8.0±0.4ml, and click “exit”.
Set sample mode back to Venous.
8.14. Reagent Sensors Sensitivity Check

Set voltmeter to DC, and connect black probe to ground and the red probe to TP1, TP2, and TP3 of
cable board, monitor the relative voltage, there are the output voltage of lyse, cleaner and diluents.
When reagent is present in the liquid sensor, the voltage should be 3 - 5Vdc, when no reagent is
present in the sensor the voltage should equal to or less than 1,5Vdc, otherwise adjust the VR1, VR2
or VR3.
8.15. Channels Gain Adjustment

This procedure is provided during technical training from SFRI. Gains are factory adjusted and don’t
need to be touched in the field.
8.16. Aperture Baseline Voltage Setting

8.16.1. ENTER INTO FACTORY MODE
Click “Service”—“Special”, in the pop-up message box input password: “6666”, click OK, press
F12 on the keyboard, to make the host enter into “Factory” mode “the system interface above
should display “factory”
8.16.2. BLANK TEST

Under the venous mode, run 5 backgrounds, blank test (press start switch without presenting
any sample). Each background result should be in specs. If the blank tests are out of tolerances,
cleaning or strong cleaning procedure may be required, as well as burn function.
8.16.3. CHANNEL DATA MONITOR

After each blank test, press F5, F6 and F7 in order to monitor the pulse graph and data amount
for WBC channel, RBC channel and PLT channel, the pulse graph of each channel should have
no disturb noise “the good pulse picture should smooth ”, the data amount should meet the
requirement below.
WBC ≤200KB (Displayed 200 000)

RBC ≤100KB
PLT ≤300KB
8.16.4. APERTURE BASELINE VOLTAGE SETTING
Record the W.H.V.S. and R.H.V.S. after each test, calculate the average of 5 times W.H.V.S. and
R.H.V.S., and input into the Parameters (click Service----Special, in the pop-up message box
input WBC Hole and RBC Hole under password 6161)
Picture 8-6

9. MAINTENANCE
9.1. User maintenance

9.1.1. DAILY MAINTENANCE
ü Ask the user to perform a background before starting the working day. Check that the result is
in range.
ü Run a QC SFRI bloodtrol and check that the result is in target.
ü At the end of the day, perform a Shutdown using the Diluclair when the instrument prompts
you.
ü Clean the unit panels with neutral detergent. Clean the screen, keyboard and mouse as
requested with adequate cleaner.
9.1.2. WEEKLY MAINTENANCE
At the end of a working week, it is recommended to run the “Maintenance” procedure,

launching it from “Mechanic” menu, and using HemaclairTM
9.1.3. ON DEMAND MAINTENANCE
For any brake down, malfunction, that the user is not able to handle, the end user
representative will have to contact the local SFRI representative technical service, in order to
proceed without delay to the relevant repair actions.

9.1.4. MAINTENANCE LOG
Frequency Operation Reagent / Material Potential trouble

Incorrect backgrounds
Run
Daily Diluclair H18 Inaccurate results
“shut down”
Clogs in fluidic
Clean up Neutral detergent
Daily Contamination
front panel Cloth
Abnormal results
Run
Weekly Hemaclair H18 Incorrect backgrounds
“enhanced flush”
Clogs in fluidic
Abnormal results
Run
Weekly Diluclair H18 Incorrect backgrounds
“maintain program”
Clogs in fluidic
Electronic troubles
Clean up Overheat
Monthly Brush
the air filter Abnormal counts
Clogs
Electronic troubles
Clean up Neutral detergent Abnormal counts
Monthly
the analyzer Cloth Clogs
Biological contamination
Table 9-1
Specific cleaning solutions:

Diluclair H18 and Hemaclair H18.
Risk of non use and benefit of use.
Diluclair H18:
Name Diluclair H18
Presentation 60ml
code HSC301
exp 1 year from date of manufacture
Description Diluclair H18 is a concentrated enzymatic cleaner that
will increase efficiency of the cleaning. The proteolytic enzyme contained
will eliminate all traces of blood in the fluidic.
Benefit Regular use will significantly decrease the risk of
clogging and of incorrect backgrounds.
Risk This will increase the consumption of reagents and it
will provoke a waste of time due to more frequent cleanings of the
instrument and especially the fluidic.

Hemaclair H18:
Name Hemaclair H18
Presentation 60ml
code HSC101
exp 1 year from date of manufacture
Description Hemaclair H18 is a hypochlorite solution mixed with
tensioactive product specifically designed for the emergency cleaning of
the fluidic in case of clogs and the cleaning of the enzyme contained will
eliminate all traces of blood in the fluidic.
Benefit Regular use will significantly decrease the risk of
clogging and of incorrect backgrounds.
Risk This will increase the consumption of reagents and it
will provoke a waste of time due to more frequent cleanings of the
instrument and especially the fluidic.

9.2. SFRI Technical Maintenance
SFRI strongly recommends performing a complete preventive maintenance at least once a year, or
every six months if the number of samples per day is equal or higher than 30.
This maintenance has to be performed by a trained service engineer working for an official SFRI
distributor. Any authorized service engineer is able to prove his habilitation by presenting an official
SFRI authorization document.
The main operations that have to be performed during preventive maintenance (normally performed
in a three hours), are summarized in the PM (Preventive Maintenance) check list bellow.
9.2.1. PREVENTIVE MAINTENANCE PROCEDURE

9.2.1.1. TOOLS:
Ultrasonic Cleaning Device

Distilled water
Hemaclair / Diluclair

ITEM DESIGNATION: Preventive Maintenance Kit H18 light-1 year
ITEM CODE: PMK0201
9.2.1.2. PROCEDURE
Replace seals and cleaning
Vacuum Pump
The seal of the vacuum pump piston must be replaced after one year (or after 6 months if number of
samples / day > 30), to ensure proper functioning (stable and accurate vacuum counting value).
Pictures 9-1 & 9-2
1/ Disassemble the bearings bracket, by unscrewing the 3 screws, which allow you to take out easily
the rod and the piston from the block (1, 2 and 3).
1 2 3
Picture 9-3
2/ Take out the tubes from the vacuum pump, push up gently the rod and the piston to take them out
from the block.

Pictures 9-4 & 9-5
3/ Disassemble the parts to clean.
4/ Clean the vacuum pump piston with an ultrasonic washing device or distilled water.
Picture 9-6

5/ Clean the rod and the gear from old 6/ Unscrew the 4 screws on the top of the block
grease and lubricate again.
Pictures 9-7 and 9-8
7/ Take out the seal from the block and clean the vacuum pump with ultrasonic washing device or
distilled water.
8/ Replace the seal with a new one. Re-assemble the parts and respect the order of assembling.
Be careful with the sense of the seal when you replace it

1.2. Dilution Block
The seals of the dilution block must be replaced after one year (or after 6 months if number of samples
/ day > 30), to ensure the proper functioning.
1/ Remove the retaining Teflon bracket, by unscrewing the two screws.
2/ Take out gently the two pistons (Lyse piston and Diluten piston) from the block by pushing down
the metal part to create a space and the pistons for disassembling from the block.
3/ Unscrew the five screws to remove Teflon bracket covering seals and o-ring.

4/ Pull out the sample piston from its chamber.
5/ Use the sample piston to turn the cable gland. “a quarter turn!”
6/ Take out the Diluents and Lyse seals from the block.
7/ Replace the seals with new spare parts.

Pictures 9-19, 9-20 and 9-21
8/ Clean all the parts of the dilution block by using the cleaning ultrasonic device or any equivalent
cleaning equipment. Re-assemble the sample piston as shown.
Picture 9-22
MANDATORY: respect the de-assembling / assembling order.

1. RBC and WBC Chamber
In case the RBC chamber must be cleaned, you have to follow the bellow procedure.
1/ Unscrew the screw to disconnect the electrical grounding wire (black cable) from the chamber (not
forget to disconnect also from the CCU Board)
Pictures 9-23 & 9-24
2/ Unscrew screws behind to de-sassemble the block.
3/ Check the integrity of the chamber (no cracks in the plastic).

4/ Clean the RBC chamber using an ultrasonic device.
5/ Clean the ruby and the silicon spacers with the ultrasonic device or Hemaclair.
CAUTION: there is only one correct way to place the ruby. Please refer to draft bellow.
Picture 9-29

1.4. Washing Head
The seal of the washing head must be replaced after one year (or after 6 months if number of samples
/ day > 30), to ensure the proper functioning.
1/ Take out the tubes from the washing head (2 tubes) and unscrew the two screws on the top of the
washing head (2 screws)
2/ gently push down the washing head, to take it out from the sample probe.
Disassemble the top part from the block to take out the seal from the washing head.
3/ Clean the parts of washing head with ultrasonic device.

4/ Replace the O-Ring with a new one.
END OF PROCEDURE

10. Troubleshooting
This chapter gives instructions for identifying, troubleshooting, and correcting instrument problems. If
you cannot solve the problems according to this guidance or you wish to get more information, please
contact your local distributor.
SYMPTOMES CAUSES ACTIONS

HGB Bubble The system sends an HGB bubble ü Check for bubble presence in the
when the voltage given by the WBC chamber (it is normal to have
sensor is bellow 4,1 Vdc. This bubble in the top of the liquid
situation may be due to an level).
optical, fluidic or electronic ü Check for WBC chamber integrity
problem. (no cracks)
ü If fluidics and optics are ok, adjust
the HGB blank value in CCU board
HGB Lamp Failure Blank voltage read is above 4,8 ü Check the chamber for any foreign
Vdc. A foreign body inside the body presence
chamber may be a possible ü Check WBC Chamber cleanliness
cause. An electronic problem on ü Replace WBC Chamber
the CCU, or electrical contact on ü If fluidics and optics are ok, adjust
the cables of the WBC chamber. the HGB blank value in CCU board
RBC CLOG Base line voltage is higher than ü Diluent temperature is cold
reference value. ü Aperture is getting clogged,
perform Enhance Flush
WBC CLOG Base line voltage is higher than ü Diluent temperature is cold
reference value. ü Aperture is getting clogged,
perform Enhance Flush
Motor X Stop Busy Number of steps performed by ü No movement : check power to
XX Busy the motorcontroller is higher motor
than expected to reach home ü Mechanical obstruction : clean
position. and lubricate
ü Home sensor out of order :
replace
ü CCU board defective : replace
Temp Low Room temperature below lower ü Check room temperature and
limit set in the system. regulate as required
ü Check temperature sensor
Temp High Room temperature above higher ü Check room temperature and
limit set in the system regulate as required
ü Check temperature sensor
Lyse Empty Voltage sent by the optical sensor ü Check if the reagent is properly
is below limit primed: if not trouble shoot. If it
is, check the adjustment
ü Readjust, or replace the sensor.
Diluent Empty Voltage sent by the optical sensor ü Check if the reagent is properly
ü Readjust, or replace the sensor.

Cleaner Empty Voltage sent by the optical sensor ü Check if the reagent is properly
Readjust, or replace the sensor.
Waste Full The electrodes of the waste ü Check for real waste full situation
sensor are shortcutted ü If not, replace the waste sensor
ü Check if the problem comes from
the CCU board.
Table 10-1

Appendices – TSB

Appendix 1: Safety Symbols
SYMBOL WARNING CONDITION ACTION
Biohazard: Consider all materials Wear standard laboratory attire,
(specimens, reagents, controls, glove and follow safe laboratory
calibrators, or components that contain procedures when handling any
or have contacted human blood) as being material in the laboratory.
potentially infectious.
Electrical Shock Hazard: The electrical Before continuing, unplug the BA-88
warning icon alerts users to the analyzer from the electrical outlet.
possibility of electrical shock in the
described activity or at the posted
location.
Probe Hazard: The probe may contain Avoid any unnecessary contact with
biohazard materials, including controls the probe and probe area.
and calibrators.
NOTE: Indicates to the operator
information of importance in the
procedure to be performed. This heading
is also used to indicate specific sample
handling techniques that are helpful in
operating the instrument.
CAUTION: Indicate to the operator
information of importance that could
result in damage to the instrument or
affect the test due to improper operation
if these instructions are not followed.
WARNING: Indicate to the operator
information regarding potential hazards
that may cause personal harm to the
operator if these instructions are not
followed.

Appendix 2: Preventive Maintenance Check List

Appendix 3: Passwords
Menu Sub-Menu Password Key Comment

Service Mechanic 6100 na Mechanic Diagnosis Menu:
- Valves
- Electronics check (CCU board
functions)
- Motors
- Data transfer
Service Special 6100 na Instrument counter:
- Accumulative count time (real
counting time only, giving
number of counting cycles)
- Accumulative run time
(instrument on)
- Count times (number of cycles
performed since last power on)
- Run time (total time instrument
is powered on, since last power
on)
Service Special 6161 Parameters Adjust:
- Mechanics Adjustments
- Fluidics Adjustments
Service Special 6666 F9 Continuous Run:
- Series = the instrument
Service Special 6666 F12 Factory Mode:

Appendix 4: The Fluidic Cycle Step by Step
Fluidic Graph
Picture A4-1

1st Step: Aspirating Sample Blood.
Picture A4-2
2nd Step: Cleaning External Side of Sample Probe.
Picture A4-3

3rd Step: Emptying the Vacuum Pump.
Picture A4-4
4th Step: Emptying the WBC Chamber.
Picture A4-5

5th Step: Emptying the Vacuum Pump.
Picture A4-6
6th Step: Dispensing diluent in WBC Chamber.
Picture A4-7

7th Step: Empty the Blood in WBC chamber.
Picture A4-8
8th Step: Creating air pressure in the Vacuum Pump.
Picture A4-9

9th Step: Mix blood and diluent in WBC chamber with air pressure.
Picture A4-10
10th Step: Empty the RBC chamber.
Picture A4-11

11th Step: Empty the Vacuum Pump.
Picture A4-12
12th Step: Aspiration of Blood and Diluent (Mixed) from the WBC chamber.
Picture A4-13

13th Step: Clean Sample Needle.
Picture A4-14
Picture A4-15

15th Step: Put diluent in RBC chamber
Picture A4-16
16th Step: Empty the blood and diluent (Mixed) in RBC chamber and add lyse in WBC chamber.
Picture A4-17

17th Step: Creating air pressure in the Vacuum Pump.
Picture A4-18
18th Step: Mix RBC/WBC chamber with air pressure.
Picture A4-19

19th Step: Counting.
Picture A4-20
20th Step: Empty the WBC chamber.
Picture A4-21

Picture A4-22
22th Step: Put Diluton in WBC chamber.
Picture A4-23

23th Step: Empty the RBC chamber.
Picture A4-24
Picture A4-25

25th Step: Put Diluton in RBC chamber.
Picture A4-26
26th Step: Clean the counting line.
Picture A4-27

Picture A4-28
END OF PROCEDURE

Appendix 5: User Maintenance Schedule Sheet

Appendix 6: Spare parts list
Item Code Item Designation
S020001 CPU Board (w/o memory stick w/o CF card)
S020001 CPU Board (w/o memory stick w/o CF card)
S020001-02 CPU Board V2 6151 (w/o memory stick w/o CF card)
S020001-03 CPU Board V2 6154 (w/o memory stick w/o CF card)
S020002 CF Card for H18 V1 - Software 3.0.2.0.01E
S020002-01 CF Card for H18 V1 - Software 3.0.2.1.01E
S020002-02 Disc On Module for H18 V2 - Software 3.0.2.02.6108E
S020002-03 Disc On Module for H18 V2 - Software 3.0.2.10.6108E
S020002-04 CF Card for H18 V2 - Software 3.02.01.6J08E.09.09.09
S020003 CCU Board V1
S020003-02 CCU Board V2
S020004 Voltage control board (V1 only)
S020005 Optical Switch ITR 8102 (COMMUN, V1)
S020005-02 Optical Switch ITR 8102 (V2)
S020006 Optical Switch ITR 8103 (Needle, V1 only)
S020007 2 Way Valve
S020008 3 Way Valve
S020009 WBC Chamber Assy
S020010 RBC Chamber Assy
S020013 Reagent Detection Sensor (V1, Optical)
S020013-02 Reagent Detection Sensor (V2, Optical)
S020014 MTB Board - Volumetric Metering Board (V1 only)
S020015 Glass Measuring Tube (V1 only)
S020019 H18 Burn Transformer (220V-110V)
S020020 H18 Inverter (V1 Only)
S020021 H18 Step Motor
S020022 H18 Vaccum pump Assembly
S020023 H18 Dilution Machine
S020024 H18 Dilution Block
S020025 H18 Sample Probe
S020026 H18 Probe Washing Head (2 lines)
S020026-02 H18 Probe Washing Head (3 lines)
S020028 H18 Thermal Printer Assy
S020029 Start Switch Assembly
S020030 Seal Lyse Piston
S020032 Seal Diluent Piston
S020033 Seal Vacuum/pressure pump Piston
S020034 O-Ring Probe Washing Head
S020035 H18 LCD Display Assy

Item Code Item Designation
S020036 Instrument Foot
S020040 Fuse T3,15A / 250 V / 5 x 20 mm (X10)
S020041 Diluent Plunger Stick
S020042 Lyse Plunger Stick
S020043 Ruby Red Blood Cell
S020044 Ruby White Blood Cell
S020045 H18 Front Panel
S020046 Hemoglobin Measuring Lamp
S020047 Printer for H18 Light (Obsolète)
S020049 Diluent connection tube (complete line)
S020050 Lyse connection tube (complete line)
S020051 Rinse connection tube (complete line)
S020052 Waste connection tube (complete line)
S020053 Metal braket with bearings for pumps
S020054 H18 Vacuum Pump Gear
S020055 H18 Driver Belt
S020056 H18 Switching Power Supply
S020057_ Bar Code Reader PS2
S020058 Tube Tygon S50HL 2,4 (3/32) x4 (5/32) mm
S020059 Tube Tygon S50HL 1,6 (1/16) x 3,2 (1/8) mm
S020060 O-ring Sample Piston
S020061 SDRAM for CPU Board
S020062 H18 Sample Machine (V1)
S020062-02 H18 Sample Machine (V2)
S020063-02 Liquid Sensor Adj. / Connection Distribution Board (V2 only)
S020064 Vacuum Pump Machine
S020065 Rod for Vacuum Pump
S020066 Rod for Dilution Machine
S020067 LCD Adapter Board
S020068 TFT Cable CPU 615X
S020068-02 TFT Cable CPU E8D
S020070 Contact à sertir femelle pour MOLEX 5-PIN
S020071 H18 Inverter Power Supply Delay
S020072 H18 Light Keyboard
S020073 High Voltage Inverter Board
S020074 Tube Tygon S50HL 2,4 (3/32) x 4 (5/32) mm (15 meters)
S020075 Tube Tygon S50HL 1,6 (1/16) x 3,2 (1/8) mm (15meters)
S020076 Tube PTFE, Sample Line, H18 Light V1 (1m)
S020077 H18 Waste Tool - BNC Load 50 ohms


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Revision History

Version Date Modification

H18 Light V2_Service Manual_English_V13.00 | 3

2. INSTALLATION PROCEDURE ....................................................... 19

3. THEORY OF OPERATION ............................................................ 25

4. FLUIDICS SYSTEM ........................................................................ 27

H18 Light V2_Service Manual_English_V13.00 | 5

7. SERVICE SOFTWARE ................................................................... 53

8. ADJUSTMENTS AND CONTROLS ................................................ 64

6 | H18 Light V2_Service Manual_English_V13.00

10. Troubleshooting ......................................................................... 85

Appendices – TSB ............................................................................... 87

H18 Light V2_Service Manual_English_V13.00 | 7

Content of this manual is subject to changes without prior notice.

Responsibility on the Manufacturer Party

H18 Light V2_Service Manual_English_V13.00 | 9

Workmanship and Materials

Safety, Reliability and Performance

10 | H18 Light V2_Service Manual_English_V13.00

H18 Light V2_Service Manual_English_V13.00 | 11

H18 Light V2_Service Manual_English_V13.00 | 13

1.1. Front Face View

H18 Light V2_Service Manual_English_V13.00 | 15

16 | H18 Light V2_Service Manual_English_V13.00

1.3. System Specifications

H18 Light V2_Service Manual_English_V13.00 | 17

Sample Volume Reagent Volume per test

Electrical input x 1 VGA x 1

On board fast thermal printer, 57.5 mm wide paper, recording width 48 mm

1.3.6. LINEARITY AND PRECISION

PARAMETERS LINEARITY RANGE PARAMETERS LINEARITY RANGE

18 | H18 Light V2_Service Manual_English_V13.00

2.1. Pre-Installation on Site Inspection

2.1.2. LOCATION REQUIREMENTS

2.1.3. POWER REQUIREMENTS

2.2. Pre-Installation Instrument Inspection

2.3. Unpacking / Installing the H18 Light

DO NOT SWITCH ON THE UNIT BEFORE READING COMPLETELY THIS SECTION

H18 Light V2_Service Manual_English_V13.00 | 19

20 | H18 Light V2_Service Manual_English_V13.00

xii. Place the reagents near the instrument.

DO NOT INCREASE THE LENGTH OF ANY OF THE REAGENT PICKUP TUBE

H18 Light V2_Service Manual_English_V13.00 | 21

DO NOT INCREASE THE LENGTH OF THE WASTE TUBE ASSEMBLY.

DISPOSE OF WASTE IN ACCORDANCE WITH GOVERNMENT REGULATIONS.

22 | H18 Light V2_Service Manual_English_V13.00

PARAMETERS PRECISION CV%

SFRI Blood Control:

NOTE: NEVER CALIBRATE A SYSTEM WHICH IS NOT STABLE

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3.1. Counting Blood Cells

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Where U is the voltage, R the resistance and I the current.

A constant current generator is applied to the electrodes of two chambers separated by

Where U is the voltage and ps the particule size.

3.1.2. TREATING THE SIGNAL

3.2. HGB reading

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4.1. Fluidics phases

CAUTION: Vacuum value is not controlled by the system. It is important to run

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You can find an accurate explanation of the fluidics phases in