BSA STANDARD CURVE

ug/ml OD
BSA
0 \\
25
300 0.282
20 500 0.504
f(x) = 20.9278256715875 x − 1.54477287719219
R² = 0.997650052287257 750 0.656
15
1000 0.558
10 1500 0.097
20000 1.081
5

0
0.000 0.200 0.400 0.600 0.800 1.000 1.200

SAMPLE 1a MISURAZIONE 2a MISURAZIONEO.D.

4 0.383 0.399 0.391
6 0.385 0.369 0.377
7 0.405 0.423 0.414
8 0.374 0.353 0.3635
10 0.336 0.343 0.3395
11 0.412 0.422 0.417
12 0.442 0.47 0.456
13 0.356 0.398 0.377
14 0.567 0.605 0.586
15 0.369 0.405 0.387
16 0.31 0.277 0.2935
17 0.432 0.447 0.4395
18 0.63 0.627 0.6285
19 0.418 0.43 0.424
20 0.467 0.499 0.483
22 0.506 0.518 0.512
25 0.383 0.388 0.3855
26 0.452 0.426 0.439
27 0.429 0.461 0.445
28 0.5 0.54 0.52
32 0.331 0.353 0.342
33 0.527 0.561 0.544
34 0.468 0.421 0.4445
35 0.359 0.326 0.3425
36 0.456 0.435 0.4455
Background STANDARD CURVE - BACKGROUND
\\ OD pg/ml
1.032 20
0.531 10
0.34 5
0.198 2.5
0.169 2
0.132 1.5

O.D. - BACKGRO µg/µl 10 gamm

0.391 6.638048 1.50647
0.377 6.345056 1.57603
0.414 7.119392 1.40461
0.3635 6.062528 1.64948
0.3395 5.560256 1.79848
0.417 7.182176 1.39234
0.456 7.998368 1.25026
0.377 6.345056 1.57603
0.586 10.719008 0.93292
0.387 6.554336 1.52571
0.2935 4.597568 2.17506
0.4395 7.653056 1.30667
0.6285 11.608448 0.86144
0.424 7.328672 1.3645
0.483 8.563424 1.16776
0.512 9.170336 1.09047
0.3855 6.522944 1.53305
0.439 7.642592 1.30846
0.445 7.76816 1.28731
0.52 9.33776 1.07092
0.342 5.612576 1.78171
0.544 9.840032 1.01626
0.4445 7.757696 1.28904
0.3425 5.62304 1.7784
0.4455 7.778624 1.28557
50 gamma 25 gamma 15 gamma 60 gamm30 gamm20 gamm40 gamm
7.5323348 3.7661674 2.2597004 9.0388 4.5194 3.01293 6.02587
7.8801511 3.9400755 2.3640453 9.45618 4.72809 3.15206 6.30412
7.0230716 3.5115358 2.1069215 8.42769 4.21384 2.80923 5.61846
8.2473846 4.1236923 2.4742154 9.89686 4.94843 3.29895 6.59791
8.9923917 4.4961959 2.6977175 10.7909 5.39544 3.59696 7.19391
6.9616785 3.4808392 2.0885035 8.35401 4.17701 2.78467 5.56934
6.2512753 3.1256376 1.8753826 7.50153 3.75077 2.50051 5.00102
7.8801511 3.9400755 2.3640453 9.45618 4.72809 3.15206 6.30412
4.6646108 2.3323054 1.3993832 5.59753 2.79877 1.86584 3.73169
7.6285378 3.8142689 2.2885613 9.15425 4.57712 3.05142 6.10283
10.875315 5.4376575 3.2625945 13.0504 6.52519 4.35013 8.70025
6.5333378 3.2666689 1.9600013 7.84001 3.92 2.61334 5.22667
4.307208 2.153604 1.2921624 5.16865 2.58432 1.72288 3.44577
6.8225185 3.4112592 2.0467555 8.18702 4.09351 2.72901 5.45801
5.838786 2.919393 1.7516358 7.00654 3.50327 2.33551 4.67103
5.4523629 2.7261815 1.6357089 6.54284 3.27142 2.18095 4.36189
7.6652505 3.8326253 2.2995752 9.1983 4.59915 3.0661 6.1322
6.542283 3.2711415 1.9626849 7.85074 3.92537 2.61691 5.23383
6.4365307 3.2182653 1.9309592 7.72384 3.86192 2.57461 5.14922
5.3546032 2.6773016 1.606381 6.42552 3.21276 2.14184 4.28368
8.9085653 4.4542827 2.6725696 10.6903 5.34514 3.56343 7.12685
5.0812843 2.5406421 1.5243853 6.09754 3.04877 2.03251 4.06503
6.4452126 3.2226063 1.9335638 7.73426 3.86713 2.57809 5.15617
8.8919873 4.4459936 2.6675962 10.6704 5.33519 3.55679 7.11359
6.4278721 3.213936 1.9283616 7.71345 3.85672 2.57115 5.1423
35 gamma
5.27263
5.51611
4.91615
5.77317
6.29467
4.87317
4.37589
5.51611
3.26523
5.33998
7.61272
4.57334
3.01505
4.77576
4.08715
3.81665
5.36568
4.5796
4.50557
3.74822
6.236
3.5569
4.51165
6.22439
4.49951