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  • Laboratory Assessment 5a. Three-Point Test Cross

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    Claraine Ladia
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    This document outlines a genetics laboratory module involving a three-point cross problem to determine gene order and distance on a chromosome. Students are provided genotype frequencies from a population and must complete tables to identify the parental gametes, double and single crossovers, and proper gene order. They then calculate the genetic distances at each single crossover region and draw the linkage map with a conclusion.

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    Genetic mapping

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    Laboratory Assessment 5a. Three-point Test Cross

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    This document outlines a genetics laboratory module involving a three-point cross problem to determine gene order and distance on a chromosome. Students are provided genotype frequencies from a population and must complete tables to identify the parental gametes, double and single crossovers, and proper gene order. They then calculate the genetic distances at each single crossover region and draw the linkage map with a conclusion.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    Download as pdf or txt
    12 views3 pages

    Laboratory Assessment 5a. Three-Point Test Cross

    Uploaded by

    Claraine Ladia
    This document outlines a genetics laboratory module involving a three-point cross problem to determine gene order and distance on a chromosome. Students are provided genotype frequencies from a population and must complete tables to identify the parental gametes, double and single crossovers, and proper gene order. They then calculate the genetic distances at each single crossover region and draw the linkage map with a conclusion.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 3

    SCORE/

    Name: REMARKS
    Course/Year/Section:
    Lab Schedule:
    Date of Submission:

    AGRIC 3115 – GENETICS FOR AGRICULTURE (Laboratory)

    Laboratory Module 5a.


    PREDICTING POPULATION OF A THREE-POINT
    CROSS MAP
    Part I. Problem Solving using Three-point cross

    1.1 A population was found to have the following genotypes with their corresponding
    frequencies. Identify the three-point gene map by completing the tables and computing for
    the distance between the genes.

    Genotype Observed Number Type of Gametes


    B Gl St 235
    b gl st 270
    B gl st 40
    b Gl St 48
    B gl St 60
    b Gl st 62
    B Gl st 7
    b gl St 4
    Total 726

    1.2 Complete the table below using the data provided above
    Category of the genotypes observed and their frequencies.
    Type of Gamete Genotype Frequency
    Parentals

    Double cross overs

    Single cross over at region


    I
    Single cross over at region
    II

    Proper gene order

    1.3. Questions to Answer:

    1.3a. Compute the gene distance at SCO I

    1.3b. Compute the gene distance at SCO II

    1.3c. Show the linkage map and Conclusion

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