Professional Documents
Culture Documents
Physiological Determinants of Elite Mountain Bike
Physiological Determinants of Elite Mountain Bike
Jacob Bejder, Thomas Christian Bonne, Michael Nyberg, Kim Anker Sjøberg
& Nikolai Baastrup Nordsborg
To cite this article: Jacob Bejder, Thomas Christian Bonne, Michael Nyberg, Kim Anker
Sjøberg & Nikolai Baastrup Nordsborg (2019) Physiological determinants of elite mountain
bike cross-country Olympic performance, Journal of Sports Sciences, 37:10, 1154-1161, DOI:
10.1080/02640414.2018.1546546
SPORTS PERFORMANCE
CONTACT Nikolai Baastrup Nordsborg nbn@nexs.ku.dk Department of Nutrition, Exercise and Sports (NEXS), University of Copenhagen, Universitetsparken 13,
Copenhagen DK-2100, Denmark
© 2018 Informa UK Limited, trading as Taylor & Francis Group
JOURNAL OF SPORTS SCIENCES 1155
performance. If so, this type of testing would be of high benefit for Measurements
coaches and athletes.
Muscle mass
Surface thigh length (L, [cm]; distance from middle point
Methods between anterior superior iliac spine and the pubic tubercle
to patella upper edge) was measured in triplicates together
Participants with circumferences and lateral skinfolds at 1/4 (O1), 1/2 (O2)
Eleven Danish elite male Olympic distance cross-country and 3/4 (O3) of L. Thigh volume was calculated from the
mountain bike athletes with a mean ± standard deviation averages (Jones & Pearson, 1969).
(SD) age, height and body mass of 19 ± 2 years, 182 ± 6 cm
and 70.2 ± 7.2 kg participated. All athletes competed at V ¼ L ð12πÞ1 ðO1 Þ2 þðO2 Þ2 þðO3 Þ2
a national or international elite level. The group contained
a multiple World Champion, World Cup winner as well as ðS 0:4Þ21 L O 103
two athletes with multiple national championships.
The analysis was based on data obtained in the general Quadriceps femoris muscle mass (M, [kg]) was determined
testing of the athletes and such did not require ethical as M = 0.307 × V + 0.353
approval as judged by the local ethical committee (ref.
#17017139). All participants gave their informed consent to
the use and presentation of their individual data. The study Counter-movement jump, maximal voluntary contraction
adheres to the Declaration of Helsinki II. and rate of force development
After 10 min cycling ergometer warm up at ~150 W (Monark,
Varberg, Sweden) three maximal counter-movement jumps
Design were performed using arm-swing and self-determined optimal
A schematic overview of the protocol is provided in Figure 1. width and depth of the legs. The athlete marked the adjacent
Briefly, “test day 1” was initiated after fasting for at least wall as high up as possible during standing and during the
one hour. Resting blood samples were collected and quadri- jump. Jump height was defined as the difference between
ceps femoris mass was estimated by anthropometric measure- marks.
ments (Bangsbo, Gollnick, Graham, & Saltin, 1991) and body Next, m. quadriceps femoris maximal voluntary contraction
composition determined by dual-energy x-ray absorptiometry and rate of force development was determined in a seated
(DEXA) scanning (DPX-IQ Lunar, Lunar Corporation Madison position with a 90° angle in hip and knee. The right ankle was
Wisconsin, USA). Subsequently, counter-movement jump strapped to a calibrated strain gage sampling at 1 kHz, which
height and m. quadriceps femoris maximal voluntary contrac- was connected to an amplifier and a data acquisition system
tion force as well as rate of force development of was mea- (ADInstruments, Oxford, United Kingdom). The athlete was
sured. Immediately after, a 4 × 30 s all-out cycling test was instructed to produce the highest amount of force as fast as
completed. Finally, after at least 45 min of rest the blood possible during three 5 s maximal isometric attempts sepa-
volume and Hbmass were determined (Schmidt & Prommer, rated by 55 s of rest. The maximal voluntary contraction force
2005). At “test day 2”, VO2peak was determined. At “test day was determined as the highest recording during any of the
3” one-legged knee-extensor maximal work capacity and max- three attempts and the rate of force development was calcu-
imal femoral artery blood flow was measured. Test day 1 and 2 lated as the maximal slope in a 200 ms window. Additionally,
were separated by 1–5 days and conducted between April and the maximal torque of m. quadriceps femoris was calculated
July while test day 3 was conducted in November. All athletes by multiplying the maximal voluntary contraction by the dis-
participated in the Danish XCO-MTB National Championship in tance from the strain gauge on the right angle to the fulcrum
June. of the knee.
Figure 1. Schematic overview of the performed measurements. MVC: maximal voluntary contraction, RFD: rate of force development, XCO-MTB: Olympic distance
cross-country mountain bike.
1156 J. BEJDER ET AL.
Repeated sprint ability competition. The test center applies a 5 min self-paced all-out
To ensure that the athlete was warmed up, the athlete exer- test with continuous VO2 measurements (Innocor CPX,
cised at 100–150 W for 30 min on an electronically braked bike Innovision, Glamsbjerg, Denmark). To evaluate the potential
(Monark, Varberg, Sweden) including 5 s of high power output difference in applied method and equipment when measuring
(4–500 W) every fifth min. Following 5 min of rest the RST was VO2peak, three elite mountain bike athletes completed both
initiated by athletes reaching > 100 rpm during unloaded protocols on a separate occasion within a period of 14 days
pedaling and subsequent instant application of 0.125 × kg but with at least 48 hours in between. The mean difference
body mass−1 braking resistance. Athletes pedaled 30 s as fast (± SD) was 59 ± 123 mL/min, ranging from 83 mL/min lower to
as possible followed by 1 min unloaded pedaling. The next 139 mL/min higher VO2peak at the national Olympic test cen-
sprint was initiated by reaching 100 rpm ~5 s prior to start. In ter. Due to differences in the peak oxygen uptake protocol
total, the athletes performed 4 × 30 s all out exercise sepa- between athletes it was not possible to include peak power
rated by 1 min. Mean power output and the highest and from the test.
lowest power output during any 5 s period was recorded
and denoted peak and minimum power output, respectively Limb exercise capacity
(Nordsborg et al., 2012). Fatigue index (FI, [%]) was determined Right leg knee-extensor exercise capacity was determined.
as FI = minimum power/peak power * 100 After 5 min of one-leg knee-extensor exercise at 12 W
(60 rpm) the load was increased by 12 W every minute until
exhaustion (rpm < 57). Peak workload (Wmax) was calculated
Hemoglobin mass and blood volume
as the last completed workload plus 12 × (t/60), with t being
Hbmass was measured using a CO-rebreathing method
the number of seconds at the final exhaustive workload.
(Schmidt & Prommer, 2005). Athletes rested for at least
Femoral arterial diameter and maximal blood flow velocity
10 min in order to stabilize blood volume (Ahlgrim et al.,
was measured at rest and continuously during the incremental
2010) and five baseline capillary blood samples were collected
one-leg knee-extensor exercise using ultrasound doppler
from a fingertip in 35 ul preheparinized tubes (Clinitubes;
(Logic E9; GE Healthcare, Copenhagen, Denmark) equipped
Radiometer, Brønshøj, Denmark). After baseline sampling and
with a linear probe operating at an imaging frequency of
at least one hour following exercise tests, the participants
8.0 MHz and Doppler frequency of 3.1 MHz (Nyberg,
inhaled a bolus of 1.0 mL per kg BW chemically pure
Christensen, Mortensen, Hellsten, & Bangsbo, 2014). The high-
(99.997%) CO (CO N47, Air Liquide, Paris, France) delivered
est obtained blood flow value (averaged over ~16s) was
via a 100 mL plastic syringe (Omnifix, Braun, Melsungen,
defined as femoral artery peak blood flow (Qmax).
Germany) to a custom designed spirometer (Blood tec GbR,
Bayreuth, Germany) creating a closed system. The system
XCO-MTB performance
contained three liters of pure oxygen which was rebreathed
The athletes completed the national championship race in
for two minutes. Five blood samples were collected 9 minutes
XCO-MTB, where all athletes competed on the same route at
following the inhalation of CO using the same technique as
the same day. The race was conducted on in the same area
during baseline sampling. Blood samples were immediately
as a previous XCO-MTB European Championship course, but
analyzed for percent carboxyhemoglobin (%COHb) and hemo-
with a higher emphasis on technical sections. The course
globin concentration ([Hb]) on an ABL 800 blood gas analyzer
consisted of dry tracks, and the course lap was approxi-
(Radiometer, Brønshøj, Denmark). A CO analyzer (Draeger,
mately 5 km with 200 m of climbing, 30% gravel road,
Luebeck, Germany) was used to evaluate if a leak of the closed
55% technical single track and 15% open field per lap. The
system occurred during the rebreathing period and measured
recruited athletes were part of two different groups, as two
any leftover CO in the spirometer and lung cavity following
athletes competed in U19 and the remaining in the elite
the rebreathing period.
group. The elite athletes completed six laps while U19 ath-
The difference in %COHb was used to calculate Hbmass
letes only completed four laps. Thus, to obtain a comparable
(Schmidt & Prommer, 2005). Blood volume (BV, [mL]) was
performance parameter for the two groups, time to comple-
calculated as BV = Hbmass (g) x 100/[Hb] (g⋅dL−1), using only
tion of four laps was utilized. Importantly, the accumulated
the average [Hb] from the resting blood samples obtained
time for completion of four rounds in the elite athletes
before any exercise.
correlated very strongly (r = 0.98) to the accumulated time
for completion of six rounds.
Peak oxygen uptake
The participants VO2peak was determined during an incremental Statistics
bicycle ergometer (Monark 839e, Varberg, Sweden) protocol Statistical analyses was performed using the IBM SPSS statis-
consisting of 6 min at 110 W, 4 min at 150 W followed by an tical software package v. 23 (IBM Corp, New York, NY, USA).
addition of 25 W per min until voluntary exhaustion. In- and Pearson’s correlation coefficient (r) was converted to
expired O2 and CO2 fractions were measured breath-by-breath a coefficient of determination (r2) and used as a measure of
(Quark CPET, Cosmed, Rome, Italy). The highest 30 s VO2 aver- the proportion of the variance in the performance measure
age was defined as VO2peak. that is predictable from the measured physiological variables.
For athletes with a tight national and international compe- The coefficient of determination was interpreted using
tition schedule (n = 5), VO2peak was obtained from the national Hopkins scale of magnitudes (www.sportsci.org). However, as
Olympic test center to minimize interference with training and the scale is based on Pearson’s correlation coefficient, the
JOURNAL OF SPORTS SCIENCES 1157
scale was converted to values corresponding to the coefficient 66.9 minutes. Seven of the elite athletes finished within top
of determination. Thus, r2 < 0.01 is interpreted as trivial, 10, the two remaining within top 20 and the U19 athletes
0.01–0.09 as small, 0.09–0.25 as moderate, 0.25–0.49 as strong, finished within top 10 as well within their class. The results of
0.49–0.81 as very strong and > 0.81 as nearly perfect. the physiological measurements are presented in Table 1.
A performance predictive model was developed by using
multiple linear regression with the backwards elimination pro-
cedure. Initially, measured variables were pooled into five Single variable prediction of XCO-MTB performance
different categories depending of their physiological proper- (Figure 2)
ties. The categories were (1) anthropometric variables (2) cen-
Variables categorized as “very strong and significant predic-
tral aerobic variables (3) peripheral aerobic variables (4) power
tors” of XCO-MTB performance were VO2peak,rel (P < 0.01); fati-
variables (5) strength variables (Table 1). Following categoriza-
gue index during the first sprint of the RST (P < 0.01); peak
tion, the three variables within each category demonstrating
power during the second sprint of the RST (P < 0.01) and one-
the highest correlation to the selected performance variable
legged knee-extensor exercise Wmax normalized to thigh mus-
were selected. If any selected variables correlated with
cle mass (P < 0.05).
r < 0.70, the variable correlating the least to the performance
Variables categorized as “strong and significant predictors”
variable was excluded. The remaining variables were analyzed
of XCO-MTB performance were peak power relative to body
by multiple linear regression. Of the resulting multiple linear
mass during the first sprint of the RST (P < 0.05), fatigue index
regression models, the model with all parameters showing
during the second sprint of the RST (P < 0.05), mean power
a variance inflation factor (VIF) < 5 and P < 0.05 was selected.
during the third sprint of the RST (P < 0.05) as well as the
If no model with VIF < 5 and P < 0.05 for all variables was
average peak power (P < 0.05) and average fatigue index
obtained, the remaining variables were inspected for collinear-
(P < 0.05) during RST.
ity, and of the two variables demonstrating the highest colli-
nearity the one demonstrating the weakest r2 to the
performance variable was excluded. The procedure was
Multi-variable predictive models for XCO MTB
repeated until VIF < 5 and P < 0.05 for all included variables.
performance (Table 2)
The level of significance was set at P < 0.05 and results are
means ± SD. The combination of VO2peak,rel, fatigue index during the first
sprint of the RST and maximal torque of m. quadriceps femoris
resulted in a model able to predict 98% of the variance in
Results
MTB-XCO performance. When exclusively including power and
The average time to complete four laps at the XCO-MTB fatigue variables of the RST in the analysis, 88% of the variance
National Championship was 60.8 ± 3.3 min with a 95% con- in XCO-MTB performance was explained by average mean-
fidence interval of [58.8; 62.7] min and ranging from 55.6 to and peak power.
Figure 2. Illustration of the predictive value of the measured physioogical values for predicting Olympic distance cross-country mountain bike (XCO-MTB)
performance. The predictive value is indicated by Pearson’s correlation of determination (r2). Statistical significant r2 are: * P < 0.05, ** P < 0.01.
making. Thus, the current and previous findings together suggest blood flow (Krustrup, Hellsten, & Bangsbo, 2004) as well as
that only little additional information about athletic potential and fatigue resistance (Nielsen et al., 2004). Thus, it appears possible
capacity is gained from more detailed physiological measure- that a high knee-extensor exercise performance capacity is
ments. The general importance of VO2peak for XCO-MTB perfor- a determinant of XCO-MTB performance. In contrast to this
mance in various conditions and different athletic populations is hypothesis, higher maximal limb blood flow (Qmax) as well
highlighted by the ~10 ml/min/kg lower VO2peak and more tech- as peak work-load in the one-legged knee extensor test (Wmax)
nical course investigated in the previous study (Novak et al., 2018). was associated with slower race times (Figure 2). However, one
Importantly, both studies demonstrate that the strongest perfor- athlete had surprisingly different values from the rest of the
mance prediction is obtained by a multi-variable approach includ- group, and exclusion of this athlete reduces the coefficient of
ing relatively simple physiological variables representing both determination to r2 = 0.07 for Qmax and r2 = 0.28 for Wmax,
aerobic power; strength and muscular power variables. demonstrating that maximal thigh blood flow or peak power is
a weak predictor of XCO-MTB performance.
a multi-variable analytical approach, 88 % of the variance in fatigue index during the 30 s all-out explained ~80 and ~65 %,
XCO-MTB performance could be explained by the RST mean respectively, of the variance in XCO-MTB performance.
and peak power. Thus, a RST seems to be a very strong, cheap
and fast way of determining the performance level of an XCO-
MTB athlete in the specific conditions. Acknowledgments
Simple regression analyses demonstrated that the initial 30 s We thank all athletes for participating in the study. Jacob Bejder was funded
all-out or the average RST peak- and mean power relative to in part by Partnership for Clean Competition and Anti-Doping Danmark.
body mass explained only 0–40 % of the XCO-MTB performance
variance, which is similar or lower compared to previous reports
(Inoue et al., 2012). Opposite of previously (Inoue et al., 2012), Disclosure statement
a high RST peak power predicted a slow race time. However, No potential conflict of interest was reported by the authors.
sprint-trained cyclists produce a higher peak power during
a 30 s sprint test compared with endurance-trained cyclists
with a higher VO2peak,rel (Calbet, De Paz, Garatachea, Cabeza ORCID
de Vaca, & Chavarren, 2003). Furthermore, fatigue index during Jacob Bejder http://orcid.org/0000-0002-9698-9188
Kim Anker Sjøberg http://orcid.org/0000-0001-6880-4804
the 1st sprint explained ~65 % of the variance in XCO-MTB
Nikolai Baastrup Nordsborg http://orcid.org/0000-0001-7077-9265
performance. Thus, both fatigue index and VO2peak,rel are strong
predictors, corresponding to results demonstrating that athletes
with high VO2peak,rel fatigue less compared with athletes with References
low VO2peak,rel (Calbet et al., 2003).
Ahlgrim, C., Pottgiesser, T., Robinson, N., Sottas, P. E., Ruecker, G., &
Schumacher, Y. O. (2010). Are 10 min of seating enough to guarantee
Methodological considerations stable haemoglobin and haematocrit readings for the athlete’s biolo-
gical passport? International Journal of Laboratory Hematology, 32(5),
Test day 1 and 2 were conducted between April and July for all 506–511.
athletes. Albeit the few months’ time span represents Bangsbo, J., Gollnick, P. D., Graham, T. E., & Saltin, B. (1991). Substrates for
a possibility for seasonal fluctuations in test results, the contin- muscle glycogen synthesis in recovery from intense exercise in man.
The Journal of Physiology, 434, 423–440.
uous intense training and competition makes it unlikely that Calbet, J. A., De Paz, J. A., Garatachea, N., Cabeza de Vaca, S., &
major changes in e.g. VO2peak occured (Sassi, Impellizzeri, Chavarren, J. (2003). Anaerobic energy provision does not limit
Morelli, Menaspa, & Rampinini, 2008). Test day 3 was performed Wingate exercise performance in endurance-trained cyclists. Journal
several months later for logistical reasons. It cannot be excluded of Applied Physiology, 94(2), 668–676.
that this influenced the performance explanatory value of the Gregory, J., Johns, D. P., & Walls, J. T. (2007). Relative vs. absolute physio-
logical measures as predictors of mountain bike cross-country race
physiological variables. However, the race season was from performance. Journal of Strength and Conditioning Research / National
March to October and no clear changes in athlete’s rankings Strength & Conditioning Association, 21(1), 17–22.
were apparent. Importantly, exclusion of test day 3 results did Huonker, M., Schmid, A., Schmidt-Trucksass, A., Grathwohl, D., & Keul, J.
not change the derived predictive model. (2003). Size and blood flow of central and peripheral arteries in highly
trained able-bodied and disabled athletes. Journal of Applied
Physiology, 95(2), 685–691.
Practical implications Impellizzeri, F., Sassi, A., Rodriguez-Alonso, M., Mognoni, P., & Marcora, S.
(2002). Exercise intensity during off-road cycling competitions. Medicine
The first choice for evaluating XCO-MTB performance is deter- and Science in Sports and Exercise, 34(11), 1808–1813.
mination of VO2peak and repeated sprint ability as demon- Impellizzeri, F. M., Marcora, S. M., Rampinini, E., Mognoni, P., & Sassi, A.
(2005). Correlations between physiological variables and performance
strated in our model. However, it is demonstrated that
in high level cross country off road cyclists. British Journal of Sports
a simple evaluation of repeated sprint ability alone can pro- Medicine, 39(10), 747–751.
vide valuable information of the expected XCO-MTB perfor- Impellizzeri, F. M., Rampinini, E., Sassi, A., Mognoni, P., & Marcora, S. (2005).
mance. Thus, sensitive and reliable testing appears available to Physiological correlates to off-road cycling performance. Journal of
all. Physical testing provides an opportunity to monitor train- Sports Sciences, 23(1), 41–47.
ing progress objectively, adjust the athlete training schedule Inoue, A., Sa Filho, A. S., Mello, F. C., & Santos, T. M. (2012). Relationship
between anaerobic cycling tests and mountain bike cross-country
or even discover new talented athletes. performance. Journal of Strength and Conditioning Research / National
Strength & Conditioning Association, 26(6), 1589–1593.
Jacobs, R. A., Rasmussen, P., Siebenmann, C., Diaz, V., Gassmann, M.,
Conclusion Pesta, D., … Lundby, C. (2011). Determinants of time trial performance
and maximal incremental exercise in highly trained endurance athletes.
The present study demonstrate that a multiple linear regression
Journal of Applied Physiology, 111(5), 1422–1430.
model explaining up to 98 % of the performance variance in an Jones, P. R., & Pearson, J. (1969). Anthropometric determination of leg fat
XCO-MTB event encompassing large temporal fluctuations in and muscle plus bone volumes in young male and female adults. The
power production, mainly by VO2peak,rel and fatigue index during Journal of Physiology, 204(2), 63p–66p.
a 30 s all-out sprint test. Moreover, the power variables of a 30 s Krustrup, P., Hellsten, Y., & Bangsbo, J. (2004). Intense interval training
enhances human skeletal muscle oxygen uptake in the initial phase of
repeated sprint test explained up to 88 % of the variance
dynamic exercise at high but not at low intensities. The Journal of
in XCO-MTB performance through a multiple linear regression Physiology, 559(Pt 1), 335–345.
model. Performance prediction by multiple linear regression was Morgan, D. W., Baldini, F. D., Martin, P. E., & Kohrt, W. M. (1989). Ten
supported by simple linear regression, where VO2peak,rel and kilometer performance and predicted velocity at VO2max among
JOURNAL OF SPORTS SCIENCES 1161
well-trained male runners. Medicine and Science in Sports and Exercise, and central adaptations of one-legged exercise. Acta Physiologica
21(1), 78–83. Scandinavica, 96(3), 289–305.
Nielsen, J. J., Mohr, M., Klarskov, C., Kristensen, M., Krustrup, P., Juel, C., & Sassi, A., Impellizzeri, F. M., Morelli, A., Menaspa, P., & Rampinini, E. (2008).
Bangsbo, J. (2004). Effects of high-intensity intermittent training on Seasonal changes in aerobic fitness indices in elite cyclists. Applied
potassium kinetics and performance in human skeletal muscle. The Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition
Journal of Physiology, 554(Pt 3), 857–870. Et Metabolisme, 33(4), 735–742.
Nordsborg, N. B., Siebenmann, C., Jacobs, R. A., Rasmussen, P., Diaz, V., Saunders, P. U., Garvican-Lewis, L. A., Schmidt, W. F., & Gore, C. J. (2013).
Robach, P., & Lundby, C. (2012). Four weeks of normobaric “live high- Relationship between changes in haemoglobin mass and maximal
train low” do not alter muscular or systemic capacity for maintaining oxygen uptake after hypoxic exposure. British Journal of Sports
pH and K(+) homeostasis during intense exercise. Journal of Applied Medicine, 47(Suppl 1), i26–30.
Physiology, 112(12), 2027–2036. Schmidt, W., & Prommer, N. (2005). The optimised CO-rebreathing
Novak, A. R., Bennett, K. J. M., Fransen, J., & Dascombe, B. J. (2018). method: A new tool to determine total haemoglobin mass routinely.
A multidimensional approach to performance prediction in Olympic European Journal of Applied Physiology, 95(5–6), 486–495.
distance cross-country mountain bikers. Journal of Sports Sciences, Schmidt, W., & Prommer, N. (2008). Effects of various training modalities
36(1), 71–78. on blood volume. Scandinavian Journal of Medicine & Science in Sports,
Nyberg, M., Christensen, P. M., Mortensen, S. P., Hellsten, Y., & Bangsbo, J. 18(Suppl 1), 57–69.
(2014). Infusion of ATP increases leg oxygen delivery but not oxygen Schmidt, W., & Prommer, N. (2010). Impact of alterations in total hemoglo-
uptake in the initial phase of intense knee-extensor exercise in humans. bin mass on VO 2max. Exercise and Sport Sciences Reviews, 38(2), 68–75.
Experimental Physiology, 99(10), 1399–1408. Stapelfeldt, B., Schwirtz, A., Schumacher, Y. O., & Hillebrecht, M. (2004).
Prins, L., Terblanche, E., & Myburgh, K. H. (2007). Field and laboratory Workload demands in mountain bike racing. International Journal of
correlates of performance in competitive cross-country mountain Sports Medicine, 25(4), 294–300.
bikers. Journal of Sports Sciences, 25(8), 927–935. UCI. (2017). Union cycliste internationale cycling regulations (Part 4 Mountain
Saltin, B., Nazar, K., Costill, D. L., Stein, E., Jansson, E., Essen, B., & Bike v). Retrieved January 1, 2017, from http://www.uci.ch/mm/Document/
Gollnick, D. (1976). The nature of the training response; peripheral News/Rulesandregulation/17/29/73/4MTB-E-1.01.2017_English.pdf