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KM (mM) 0.

5
Vmax (mM/s) 50
K1 (mM) 2
[I] (mM) 1 2 5 10

[s] Vo I Vo II Vo III Vo IV 1/s


0.5 20 16.6666667 11.1111111 7.14285714 2
1 28.5714286 25 18.1818182 12.5 1
1.5 33.3333333 30 23.0769231 16.6666667 0.66666667
2 36.3636364 33.3333333 26.6666667 20 0.5
2.5 38.4615385 35.7142857 29.4117647 22.7272727 0.4
3 40 37.5 31.5789474 25 0.33333333
3.5 41.1764706 38.8888889 33.3333333 26.9230769 0.28571429
4 42.1052632 40 34.7826087 28.5714286 0.25
4.5 42.8571429 40.9090909 36 30 0.22222222
5 43.4782609 41.6666667 37.037037 31.25 0.2
5.5 44 42.3076923 37.9310345 32.3529412 0.18181818
6 44.4444444 42.8571429 38.7096774 33.3333333 0.16666667
6.5 44.8275862 43.3333333 39.3939394 34.2105263 0.15384615
7 45.1612903 43.75 40 35 0.14285714
7.5 45.4545455 44.1176471 40.5405405 35.7142857 0.13333333
8 45.7142857 44.4444444 41.025641 36.3636364 0.125
8.5 45.9459459 44.7368421 41.4634146 36.9565217 0.11764706
9 46.1538462 45 41.8604651 37.5 0.11111111
9.5 46.3414634 45.2380952 42.2222222 38 0.10526316
10 46.5116279 45.4545455 42.5531915 38.4615385 0.1
10.5 46.6666667 45.6521739 42.8571429 38.8888889
11 46.8085106 45.8333333 43.1372549 39.2857143
11.5 46.9387755 46 43.3962264 39.6551724
12 47.0588235 46.1538462 43.6363636 40
12.5 47.1698113 46.2962963 43.8596491 40.3225806
13 47.2727273 46.4285714 44.0677966 40.625
13.5 47.3684211 46.5517241 44.2622951 40.9090909
14 47.4576271 46.6666667 44.4444444 41.1764706
14.5 47.5409836 46.7741935 44.6153846 41.4285714
15 47.6190476 46.875 44.7761194 41.6666667
15.5 47.6923077 46.969697 44.9275362 41.8918919
16 47.761194 47.0588235 45.0704225 42.1052632
16.5 47.826087 47.1428571 45.2054795 42.3076923
17 47.8873239 47.2222222 45.3333333 42.5
17.5 47.9452055 47.2972973 45.4545455 42.6829268
18 48 47.3684211 45.5696203 42.8571429
18.5 48.0519481 47.4358974 45.6790123 43.0232558
19 48.1012658 47.5 45.7831325 43.1818182
19.5 48.1481481 47.5609756 45.8823529 43.3333333
20 48.6481481 48.0609756 46.3823529 43.8333333
20.5 49.1481481 48.5609756 46.8823529 44.3333333
21 49.6481481 49.0609756 47.3823529 44.8333333
21.5 50.1481481 49.5609756 47.8823529 45.3333333
22 50.6481481 50.0609756 48.3823529 45.8333333
22.5 51.1481481 50.5609756 48.8823529 46.3333333
23 51.6481481 51.0609756 49.3823529 46.8333333
23.5 52.1481481 51.5609756 49.8823529 47.3333333
24 52.6481481 52.0609756 50.3823529 47.8333333
24.5 53.1481481 52.5609756 50.8823529 48.3333333
25 53.6481481 53.0609756 51.3823529 48.8333333
25.5 54.1481481 53.5609756 51.8823529 49.3333333
26 54.6481481 54.0609756 52.3823529 49.8333333
26.5 55.1481481 54.5609756 52.8823529 50.3333333
27 55.6481481 55.0609756 53.3823529 50.8333333
27.5 56.1481481 55.5609756 53.8823529 51.3333333
28 56.6481481 56.0609756 54.3823529 51.8333333
28.5 57.1481481 56.5609756 54.8823529 52.3333333
29 57.6481481 57.0609756 55.3823529 52.8333333
29.5 58.1481481 57.5609756 55.8823529 53.3333333
30 58.6481481 58.0609756 56.3823529 53.8333333
30.5 59.1481481 58.5609756 56.8823529 54.3333333
31 59.6481481 59.0609756 57.3823529 54.8333333
31.5 60.1481481 59.5609756 57.8823529 55.3333333
32 60.6481481 60.0609756 58.3823529 55.8333333
32.5 61.1481481 60.5609756 58.8823529 56.3333333
33 61.6481481 61.0609756 59.3823529 56.8333333
33.5 62.1481481 61.5609756 59.8823529 57.3333333
34 62.6481481 62.0609756 60.3823529 57.8333333
34.5 63.1481481 62.5609756 60.8823529 58.3333333
35 63.6481481 63.0609756 61.3823529 58.8333333
35.5 64.1481481 63.5609756 61.8823529 59.3333333
36 64.6481481 64.0609756 62.3823529 59.8333333
36.5 65.1481481 64.5609756 62.8823529 60.3333333
37 65.6481481 65.0609756 63.3823529 60.8333333
37.5 66.1481481 65.5609756 63.8823529 61.3333333
38 66.6481481 66.0609756 64.3823529 61.8333333
38.5 67.1481481 66.5609756 64.8823529 62.3333333
39 67.6481481 67.0609756 65.3823529 62.8333333
39.5 68.1481481 67.5609756 65.8823529 63.3333333
40 68.6481481 68.0609756 66.3823529 63.8333333
40.5 69.1481481 68.5609756 66.8823529 64.3333333
41 69.6481481 69.0609756 67.3823529 64.8333333
41.5 70.1481481 69.5609756 67.8823529 65.3333333
42 70.6481481 70.0609756 68.3823529 65.8333333
42.5 71.1481481 70.5609756 68.8823529 66.3333333
43 71.6481481 71.0609756 69.3823529 66.8333333
43.5 72.1481481 71.5609756 69.8823529 67.3333333
44 72.6481481 72.0609756 70.3823529 67.8333333
44.5 73.1481481 72.5609756 70.8823529 68.3333333
45 73.6481481 73.0609756 71.3823529 68.8333333
45.5 74.1481481 73.5609756 71.8823529 69.3333333
46 74.6481481 74.0609756 72.3823529 69.8333333
46.5 75.1481481 74.5609756 72.8823529 70.3333333
47 75.6481481 75.0609756 73.3823529 70.8333333
47.5 76.1481481 75.5609756 73.8823529 71.3333333
48 76.6481481 76.0609756 74.3823529 71.8333333
48.5 77.1481481 76.5609756 74.8823529 72.3333333
49 77.6481481 77.0609756 75.3823529 72.8333333
49.5 78.1481481 77.5609756 75.8823529 73.3333333
50 78.6481481 78.0609756 76.3823529 73.8333333
1/Vo I 1/Vo II 1/Vo III 1/Vo IV [S]= 2 [S]= 50
0.05 0.06 0.09 0.14 0.72727273 1.57296296
0.035 0.04 0.055 0.08
0.03 0.03333333 0.04333333 0.06
0.0275 0.03 0.0375 0.05 Grafico de Mich
0.026 0.028 0.034 0.044
50
0.025 0.02666667 0.03166667 0.04
45
0.02428571 0.02571429 0.03 0.03714286
40
0.02375 0.025 0.02875 0.035
0.02333333 0.02444444 0.02777778 0.03333333 35

0.023 0.024 0.027 0.032 30


0.02272727 0.02363636 0.02636364 0.03090909 25

Vo
0.0225 0.02333333 0.02583333 0.03 20
0.02230769 0.02307692 0.02538462 0.02923077 15
0.02214286 0.02285714 0.025 0.02857143 10
0.022 0.02266667 0.02466667 0.028
5
0.021875 0.0225 0.024375 0.0275
0
0.02176471 0.02235294 0.02411765 0.02705882 0 2 4
0.02166667 0.02222222 0.02388889 0.02666667
0.02157895 0.02210526 0.02368421 0.02631579
0.0215 0.022 0.0235 0.026 Vo I Vo II

Grafico de Lin
0.16

0.14

0.12

0.1

0.08
1/Vo

0.06
f(x) = 0.02 x + 0.02
0.04

0.02

0
0 0.5 1
f(x) = 0.02 x + 0.02
0.04

0.02

0
0 0.5 1

1/Vo I 1
1/Vo III 1
Grafico de Michaelis menden

2 4 6 8 10 12

[s]

Vo I Vo II Vo III Vo IV

Grafico de Lineweaver-Burk

= 0.02 x + 0.02

0.5 1 1.5 2 2.5

1/S
= 0.02 x + 0.02

0.5 1 1.5 2 2.5

1/S
1/Vo I 1/Vo II Linear (1/Vo II)
1/Vo III 1/Vo IV

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