PRACTICAL Name:

25 Anaerobic respiration
You need:
■ test tubes ■ liquid paraffin ■ boiled peas in cold, boiled
■ thermometer ■ Thermos flasks water containing bactericide
yeast in cold, boiled water (eg. sodium chlorate(I) 10%)
■ balloons ■
■ measuring cylinder ■ glucose in cold, boiled water
■ limewater ■ fresh peas in cold, boiled water

Anaerobic respiration (fermentation) in yeast

Balloon
1 Label three test tubes A, B, and C.
Place 20 cm3 of yeast suspended in cold, boiled water into tube A.
(Boiling removes oxygen from water.) Add a few drops of liquid
paraffin (to cover the surface of the yeast suspension). Place 20 cm3
of glucose dissolved in cold, boiled water into tube B. Add liquid
paraffin. Place 10 cm3 of yeast suspended in cold, boiled water into
tube C, then add 10 cm3 of glucose dissolved in cold, boiled water.
Mix the two together. Add a few drops of liquid paraffin.
2 Place a balloon firmly over the neck of each tube (tie with cotton Liquid paraffin
if necessary). Make sure the balloon is deflated. Put the tubes in a Yeast/glucose
warm place for 24 hours. mixture

3 Record what happens to the balloons.

Explain your observations.
Why was cold, boiled water used in this experiment?
Why was liquid paraffin added to each tube?
What gas entered a balloon?

Anaerobic respiration in peas
1 Fit two Thermos flasks with bungs through
which a thermometer has been passed. Label
the flasks A and B.
2 Fill flask A with boiled peas in cold, boiled
water containing bactericide.
Fill flask B with fresh peas in cold, boiled water.
Note the temperature of each flask.
3 Place the bung in each flask, so that air cannot
enter and leave them for a week.
Note any temperature changes daily.
4 Explain any changes which occur in the
temperature, and in the peas.
Why was one set of peas boiled?
Why were both sets of peas in boiled, cold water.
Why was bactericide added to flask A?

Your teacher will be looking for: HAZARD WARNING

■ careful and skilful use of the apparatus given
Sodium chlorate(I) solution is
■ good observation and presentation of results corrosive. AVOID SKIN CONTACT.
■ sensible conclusion which match your results WEAR EYE PROTECTION.

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